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Gene information
General information
TC010783 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5196602..5208015
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010783
    iBeetle screen iB_04908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010783 RA
      MEAPTKFLQ DEKENFVTV VSVGTDSTR TTAKCDPFS 
      PIDTKTSGY VTVLTIGDN DEECVTKDI IEEVLVYRL 
      PGERLGFGL KFEGGTKAN EFVKRLFIQ SCAPDSPAS 
      RVQSSWGKL TEGDEVLEI DCMPVNTMT RIDCVRCLK 
      DSNVVIKLL VRHFSADCK QSNDFKTRS VEDLPQVIS 
      AEKKRTPPP PPPVPPRKI PKKFYKNSQ NSQENPVPA 
      ARQINSEAA NKKKFQSPR SSGRTSHSP DIPRRDRRF 
      SDGSLGPPD AEVYVDLFS QESTQSLSE SDDTGSSIS 
      TVVDRFGSF PTTTTSSLA GSLPSTPTS VQKQIDFTN 
      LFNSYEEED FIAPLNKSI KSVASKTPE LIEKEKAEE 
      KSGNINEDG LPLQPPSCF QDAPLSYGN EDMRIIETK 
      SNEINVCES AEKKSMNSK HVENNYLNG EKTSTKNSA 
      IEDKVFKKP PVPPRSRDL INANKTNGC KDVQNIHDE 
      NMNISNLPR LVDFVPKST TRDHFENPV EIINIFLEN 
      ERRDTDYLK NEFYGDDEI DGNFDGYNN EIDVYSSKW 
      SLSSQLATI GEVEEENSQ DFGTNRSPL NSTPIVIVE 
      NADNDTIEE INTSEVKKS EGINGKMET LPSDSRQPP 
      DGHEFPDYV EVTSEQTDD KFVRENYKD LYSQELSEK 
      SVTCKSTES VNWSQNRSS FNDFSNVPI QNKSSLSKI 
      NSVSNSDLS SFKSNSFDS GDEVSLRKS SLEPTLHTR 
      SQSLIDMSS ISKQKNDRW SLLAEQRRK GYSKLKGLV 
      IPEHVSENE PTPAVNIPE IISHTTPTF VLDTKLQTS 
      ETTQGHANE IEPIALPLT SPPWTSNTS TFPKYSPAF 
      KRKSLQVYS QTKTQKPEE TETAIKSPA KTVPDCKSA 
      TLKHKSDEL RLDNLSDSP KSLESITSP TRSDCSFDY 
      VTNSLNKVK CSKQEPNRT YKETFNYVT KLHKDIGKS 
      EDESDNDSA VSSSQSSYI SRCSPPPSP TRSCEMLDY 
      ESPTRTKPE TIQDVDKYN NLQCRLLKA ASVEAINRK 
      NILASAKCR SGRDLKVGS PVIQRKYED EDSSANAIT 
      VEPETPVNP VNQLAICKQ DQSAETRNM IKEKRSISR 
      EESAPISKP LLNGDSGIK SEIAPSPNR VPEKNNLIT 
      LKPVAGSLK NFTTQLRET KIPPKSITK PSSWLKNNN 
      KSMSVTDLR KSFEKFGNA PPPLPQNIP MALSKEPKS 
      RPAKSEAKL KPETLLEKK PRSNGEVAK LETPKPATK 
      PLVKDAAEQ KQKDTPVGG NRRKVVLCP EFVGGSIGI 
      TLAGGTDYE TKEITVHKI RYGSVAYND GQLKKGDKI 
      VSINGKETT NLTHAESVE LLKEPVSKF ILIVEDGKE 
      ETLPSPQRK SFIYEPSEP KSPGPASPA KNVTHVIKF 
      TKNGAGLGF SIEGGKDSP MGDLPLRVK KIFQGGLAE 
      KNGELCVGD ELLAINDIS LTNLSRIET WSLMKKLPD 
      GNVSIHIHR
    CDS sequence
    • >TC010783 RA
      atggaagca ccgacaaaa ttcctccaa gatgaaaaa 
      gaaaatttc gtcactgta gtctcagta ggaactgat 
      tcgacccgc actacggca aaatgtgac cctttttcg 
      ccaatcgac accaaaacc tcaggatac gtcactgtc 
      ttaaccatc ggcgacaat gacgaagaa tgtgttact 
      aaagacata atagaagaa gtcctagtc tacagactt 
      cccggcgaa cgcctaggt ttcgggcta aagttcgaa 
      ggcggaact aaggcaaac gagttcgtt aaacgcctg 
      ttcatccag tcatgtgcc cccgacagt ccagcctcc 
      cgggtccag agctcctgg ggcaaacta accgaaggt 
      gacgaagtc ctagaaatc gactgcatg ccagtcaac 
      accatgaca agaatcgac tgtgtgcgg tgtctaaaa 
      gactcaaac gttgtaatt aaacttcta gtgagacat 
      tttagtgcg gactgtaaa caatcaaat gacttcaaa 
      actaggtca gtggaagat ttgccgcaa gtcataagt 
      gccgaaaaa aagagaaca ccgccacca cctccccca 
      gtacctccg agaaaaatc ccgaaaaag ttctacaag 
      aatagtcaa aacagccaa gaaaatccg gttccggca 
      gcgcgtcaa atcaattcg gaagccgca aataagaaa 
      aaattccag tcgccacgg agcagcggg cggacttca 
      cattccccc gacatcccc agacgggac aggagattt 
      tcggacggt agtctcgga ccacccgat gccgaggtt 
      tacgtcgac ttgttttcg caagaatcg acgcaaagc 
      ctcagcgaa tccgatgac acaggaagt tcgatatct 
      accgttgta gatcgattt gggtcattc ccgacaaca 
      acaacatca agtttagca ggaagtcta ccatcaaca 
      cccacgtca gttcaaaaa caaatcgat tttacgaac 
      ctcttcaac tcgtacgag gaggaggac ttcatagca 
      ccgttaaac aagagcatt aaaagtgtg gcaagcaaa 
      actcccgag ctgattgaa aaagaaaaa gccgaggaa 
      aaatcagga aacatcaac gaagacgga ctgccgctc 
      cagcctccg tcatgcttt caagatgcc cccctgagt 
      tacggaaac gaggacatg agaatcatc gagacgaaa 
      tcgaatgaa attaatgtg tgtgagtcg gcagaaaaa 
      aaatctatg aatagtaaa cacgtggaa aataattat 
      ttaaatggt gagaaaact tcgacaaaa aattctgcg 
      attgaggac aaagtgttt aaaaagcca cctgtacct 
      ccgcgatcg cgggactta atcaatgct aataaaacg 
      aatggatgc aaagatgtg caaaatatt catgacgag 
      aacatgaac atatcgaac ttgccgcgg ttagttgac 
      ttcgtccct aaatcgaca acgcgcgac cattttgaa 
      aacccggtc gagattatt aacattttt ttggaaaac 
      gaaaggaga gacactgat tatttgaaa aacgagttc 
      tatggggac gacgaaatt gacggcaat tttgacggc 
      tataacaac gagattgat gtttatagc tcaaagtgg 
      agcttatcg tcgcaattg gcgacgatc ggggaagtc 
      gaagaggaa aattcgcag gattttggc accaacaga 
      tcgccgctt aactcaacg cccatcgtg atagttgaa 
      aacgccgat aacgacacg atcgaggaa ataaacaca 
      tcagaagtg aaaaaaagc gagggtatc aatggcaaa 
      atggaaact cttccaagt gattcacgg cagccacct 
      gatggacat gagtttccc gactacgtc gaagtcact 
      agtgaacaa accgatgat aagttcgtt cgcgaaaat 
      tacaaagat ctatacagc caagagcta agcgaaaaa 
      agtgtgact tgcaagtca acagaaagt gtaaattgg 
      agtcaaaac agaagttcc tttaatgat ttttccaac 
      gtgcccata cagaataag tcttctctt tcaaagata 
      aacagtgtc tcgaacagt gatcttagt tcttttaag 
      tcgaattcg ttcgacagc ggtgatgaa gtaagttta 
      cgaaagtcc agtttagag ccaactctc catacgaga 
      tcgcaaagc ttaattgat atgtcgagc atatcaaaa 
      caaaagaac gatcgctgg agtctcctc gccgaacaa 
      agaagaaaa ggttattct aaactgaaa ggacttgtg 
      atacctgaa cacgtatct gaaaatgaa ccaactcct 
      gcggttaac atcccggaa ataataagt cacactact 
      ccaacgttc gtcctagac acaaagctt caaaccagt 
      gaaacaacc caaggccat gcgaacgaa atcgaaccg 
      attgcatta ccactaacc tcgccccct tggacttcc 
      aacacttcc accttcccc aagtattcg cccgctttc 
      aagaggaaa agtctccaa gtctattca caaacaaaa 
      actcagaag cccgaagaa acagaaact gccataaaa 
      tcgccagcc aaaactgtg cctgattgt aaaagtgcc 
      acattaaag cataagagt gacgaactt cgacttgac 
      aacctgtcg gattcccct aaatcgtta gaaagcatt 
      acctcgccg acaagatcg gactgcagc ttcgattac 
      gtcacgaat tcgttaaac aaagtcaaa tgcagcaaa 
      caagaaccc aacagaacg tacaaagaa actttcaac 
      tatgtcact aaattacat aaggatatt ggaaaatcc 
      gaggatgaa agtgacaat gattcagca gtgagttcg 
      agtcagtcc agttacatt tcgaggtgc tcgccacca 
      ccctcaccc acacgatcc tgcgaaatg ttagactac 
      gaatcaccg actcggaca aaacccgaa accattcag 
      gacgtcgac aaatacaac aatctgcag tgccgtctc 
      ttaaaagcc gctagtgtg gaagccatc aatcgcaaa 
      aacatcctg gcatcggcg aaatgccgc agtggcaga 
      gacctcaaa gttggctca ccagtgatt cagagaaaa 
      tacgaggat gaggattct tccgcaaac gctattaca 
      gttgaaccc gagactccc gtaaatcca gtaaatcaa 
      ctagcgatc tgtaaacaa gaccaatcg gcggagact 
      cgtaacatg ataaaggaa aaacgaagc ataagtcgc 
      gaggaatct gctcccatt tccaagcct ctactgaac 
      ggagatagc ggaataaaa agtgaaata gctccatcg 
      cccaataga gtccccgaa aaaaataat ttaatcacg 
      ctaaaaccg gtagccggc tctttgaaa aactttacg 
      acgcagtta cgcgaaacc aaaatccct ccaaaaagc 
      atcacaaag ccgagcagt tggctgaaa aacaacaac 
      aagtcgatg agtgtcacc gacttgcgg aaaagtttc 
      gaaaaattc ggcaacgca cccccgcct cttccccaa 
      aacatcccc atggcgctc agcaaggag ccgaaatcc 
      cgacctgct aagtcggaa gctaaactg aaacctgaa 
      actctcctt gagaaaaaa cctagaagt aatggcgaa 
      gtcgctaag ttggaaaca ccaaagcct gcaacaaaa 
      ccgctcgtg aaagatgca gccgagcaa aagcaaaaa 
      gacactcct gttggcggt aataggaga aaggtggtt 
      ctgtgcccg gaattcgtc ggggggagc attgggata 
      actctggcc ggagggacg gattacgag acaaaagaa 
      ataaccgtg cacaaaatc cgttacggt agtgtagcg 
      tataacgac ggccagttg aaaaaaggc gataaaatc 
      gtatccatt aacggaaaa gaaacgacc aatctcaca 
      cacgcggaa tctgttgaa ctgttaaaa gaaccggta 
      tccaaattt attctaatt gtggaagat ggtaaagaa 
      gagacgttg ccttcaccg cagcgaaaa tccttcatt 
      tatgaacca tccgaaccg aaatctcct gggcccgct 
      tccccggcg aaaaatgta acgcatgtg attaagttt 
      acgaaaaat ggggctggg ttagggttc agtatagaa 
      gggggcaag gattcgcca atgggagac ttgcccttg 
      cgcgtcaaa aaaatattc caaggtgga cttgccgag 
      aaaaacgga gaattgtgc gttggcgac gaactgttg 
      gctattaat gatattagc ttaacaaat ttgtcaagg 
      attgaaaca tggtcccta atgaagaag ctacctgat 
      gggaatgtc agtattcac attcatcga taa
    mRNA sequence
    • >TC010783 RA
      cccacacga ggtcaggtg tttgaagtg ggactctgt 
      gagaggtga aaacgtgaa aacagttgt ttacaatct 
      ccgcgcgag tgtcccgta tcacaatca taaacaagt 
      ccagggaaa tgtgctcga gagtctcta ggatgtttg 
      caagttgaa gaaaatgga agcaccgac aaaattcct 
      ccaagatga aaaagaaaa tttcgtcac tgtagtctc 
      agtaggaac tgattcgac ccgcactac ggcaaaatg 
      tgacccttt ttcgccaat cgacaccaa aacctcagg 
      atacgtcac tgtcttaac catcggcga caatgacga 
      agaatgtgt tactaaaga cataataga agaagtcct 
      agtctacag acttcccgg cgaacgcct aggtttcgg 
      gctaaagtt cgaaggcgg aactaaggc aaacgagtt 
      cgttaaacg cctgttcat ccagtcatg tgcccccga 
      cagtccagc ctcccgggt ccagagctc ctggggcaa 
      actaaccga aggtgacga agtcctaga aatcgactg 
      catgccagt caacaccat gacaagaat cgactgtgt 
      gcggtgtct aaaagactc aaacgttgt aattaaact 
      tctagtgag acattttag tgcggactg taaacaatc 
      aaatgactt caaaactag gtcagtgga agatttgcc 
      gcaagtcat aagtgccga aaaaaagag aacaccgcc 
      accacctcc cccagtacc tccgagaaa aatcccgaa 
      aaagttcta caagaatag tcaaaacag ccaagaaaa 
      tccggttcc ggcagcgcg tcaaatcaa ttcggaagc 
      cgcaaataa gaaaaaatt ccagtcgcc acggagcag 
      cgggcggac ttcacattc ccccgacat ccccagacg 
      ggacaggag attttcgga cggtagtct cggaccacc 
      cgatgccga ggtttacgt cgacttgtt ttcgcaaga 
      atcgacgca aagcctcag cgaatccga tgacacagg 
      aagttcgat atctaccgt tgtagatcg atttgggtc 
      attcccgac aacaacaac atcaagttt agcaggaag 
      tctaccatc aacacccac gtcagttca aaaacaaat 
      cgattttac gaacctctt caactcgta cgaggagga 
      ggacttcat agcaccgtt aaacaagag cattaaaag 
      tgtggcaag caaaactcc cgagctgat tgaaaaaga 
      aaaagccga ggaaaaatc aggaaacat caacgaaga 
      cggactgcc gctccagcc tccgtcatg ctttcaaga 
      tgcccccct gagttacgg aaacgagga catgagaat 
      catcgagac gaaatcgaa tgaaattaa tgtgtgtga 
      gtcggcaga aaaaaaatc tatgaatag taaacacgt 
      ggaaaataa ttatttaaa tggtgagaa aacttcgac 
      aaaaaattc tgcgattga ggacaaagt gtttaaaaa 
      gccacctgt acctccgcg atcgcggga cttaatcaa 
      tgctaataa aacgaatgg atgcaaaga tgtgcaaaa 
      tattcatga cgagaacat gaacatatc gaacttgcc 
      gcggttagt tgacttcgt ccctaaatc gacaacgcg 
      cgaccattt tgaaaaccc ggtcgagat tattaacat 
      ttttttgga aaacgaaag gagagacac tgattattt 
      gaaaaacga gttctatgg ggacgacga aattgacgg 
      caattttga cggctataa caacgagat tgatgttta 
      tagctcaaa gtggagctt atcgtcgca attggcgac 
      gatcgggga agtcgaaga ggaaaattc gcaggattt 
      tggcaccaa cagatcgcc gcttaactc aacgcccat 
      cgtgatagt tgaaaacgc cgataacga cacgatcga 
      ggaaataaa cacatcaga agtgaaaaa aagcgaggg 
      tatcaatgg caaaatgga aactcttcc aagtgattc 
      acggcagcc acctgatgg acatgagtt tcccgacta 
      cgtcgaagt cactagtga acaaaccga tgataagtt 
      cgttcgcga aaattacaa agatctata cagccaaga 
      gctaagcga aaaaagtgt gacttgcaa gtcaacaga 
      aagtgtaaa ttggagtca aaacagaag ttcctttaa 
      tgatttttc caacgtgcc catacagaa taagtcttc 
      tctttcaaa gataaacag tgtctcgaa cagtgatct 
      tagttcttt taagtcgaa ttcgttcga cagcggtga 
      tgaagtaag tttacgaaa gtccagttt agagccaac 
      tctccatac gagatcgca aagcttaat tgatatgtc 
      gagcatatc aaaacaaaa gaacgatcg ctggagtct 
      cctcgccga acaaagaag aaaaggtta ttctaaact 
      gaaaggact tgtgatacc tgaacacgt atctgaaaa 
      tgaaccaac tcctgcggt taacatccc ggaaataat 
      aagtcacac tactccaac gttcgtcct agacacaaa 
      gcttcaaac cagtgaaac aacccaagg ccatgcgaa 
      cgaaatcga accgattgc attaccact aacctcgcc 
      cccttggac ttccaacac ttccacctt ccccaagta 
      ttcgcccgc tttcaagag gaaaagtct ccaagtcta 
      ttcacaaac aaaaactca gaagcccga agaaacaga 
      aactgccat aaaatcgcc agccaaaac tgtgcctga 
      ttgtaaaag tgccacatt aaagcataa gagtgacga 
      acttcgact tgacaacct gtcggattc ccctaaatc 
      gttagaaag cattacctc gccgacaag atcggactg 
      cagcttcga ttacgtcac gaattcgtt aaacaaagt 
      caaatgcag caaacaaga acccaacag aacgtacaa 
      agaaacttt caactatgt cactaaatt acataagga 
      tattggaaa atccgagga tgaaagtga caatgattc 
      agcagtgag ttcgagtca gtccagtta catttcgag 
      gtgctcgcc accaccctc acccacacg atcctgcga 
      aatgttaga ctacgaatc accgactcg gacaaaacc 
      cgaaaccat tcaggacgt cgacaaata caacaatct 
      gcagtgccg tctcttaaa agccgctag tgtggaagc 
      catcaatcg caaaaacat cctggcatc ggcgaaatg 
      ccgcagtgg cagagacct caaagttgg ctcaccagt 
      gattcagag aaaatacga ggatgagga ttcttccgc 
      aaacgctat tacagttga acccgagac tcccgtaaa 
      tccagtaaa tcaactagc gatctgtaa acaagacca 
      atcggcgga gactcgtaa catgataaa ggaaaaacg 
      aagcataag tcgcgagga atctgctcc catttccaa 
      gcctctact gaacggaga tagcggaat aaaaagtga 
      aatagctcc atcgcccaa tagagtccc cgaaaaaaa 
      taatttaat cacgctaaa accggtagc cggctcttt 
      gaaaaactt tacgacgca gttacgcga aaccaaaat 
      ccctccaaa aagcatcac aaagccgag cagttggct 
      gaaaaacaa caacaagtc gatgagtgt caccgactt 
      gcggaaaag tttcgaaaa attcggcaa cgcaccccc 
      gcctcttcc ccaaaacat ccccatggc gctcagcaa 
      ggagccgaa atcccgacc tgctaagtc ggaagctaa 
      actgaaacc tgaaactct ccttgagaa aaaacctag 
      aagtaatgg cgaagtcgc taagttgga aacaccaaa 
      gcctgcaac aaaaccgct cgtgaaaga tgcagccga 
      gcaaaagca aaaagacac tcctgttgg cggtaatag 
      gagaaaggt ggttctgtg cccggaatt cgtcggggg 
      gagcattgg gataactct ggccggagg gacggatta 
      cgagacaaa agaaataac cgtgcacaa aatccgtta 
      cggtagtgt agcgtataa cgacggcca gttgaaaaa 
      aggcgataa aatcgtatc cattaacgg aaaagaaac 
      gaccaatct cacacacgc ggaatctgt tgaactgtt 
      aaaagaacc ggtatccaa atttattct aattgtgga 
      agatggtaa agaagagac gttgccttc accgcagcg 
      aaaatcctt catttatga accatccga accgaaatc 
      tcctgggcc cgcttcccc ggcgaaaaa tgtaacgca 
      tgtgattaa gtttacgaa aaatggggc tgggttagg 
      gttcagtat agaaggggg caaggattc gccaatggg 
      agacttgcc cttgcgcgt caaaaaaat attccaagg 
      tggacttgc cgagaaaaa cggagaatt gtgcgttgg 
      cgacgaact gttggctat taatgatat tagcttaac 
      aaatttgtc aaggattga aacatggtc cctaatgaa 
      gaagctacc tgatgggaa tgtcagtat tcacattca 
      tcgataatt ttttgacta tttttattt atttgtact 
      tttttaagt attgtcttg ccagactgt ttgtaatgt 
      tcttgtacg tacttattc gttgttaaa ttatttcct 
      tgtgtcagt catggttat gtgcccaca gttaaatca 
      tttatatat tttattaca tagcttatt tgtatgata 
      acattacct aaaatatct agtacagtg tatagattg 
      ttcattaat atattttta tatgaataa attattgat 
      atatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY