Status
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Gene information
General information
TC010769 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4980186..4983642
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010769
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04907

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010769 RA
        MASYSDSED VDDQDWDEF EDAVNDIDF PAPQGDMTL 
        ERAVDESQK AINFFFNNQ FLEAKSLME PYAATSIYH 
        SLGHSVFLF LEAILTFEH KSIVNASTA LKHAIQVCN 
        KQRKKNTFG ESLGKMVKR NNFDQYSEE EAHAELCHA 
        ECLLLKSML TFMEDETLS SFIKAGIKI RSCYNSYKD 
        CQQILLKSQ WDKSPSKVH FESGVKMGI GTFNLMISL 
        LPSRVIKLL EFIGFSGNK QLGLNDLVE GYKTDGLRQ 
        ILCLMTLLG YNLIVLYVL SHQEGDLKL SQEILEKQL 
        KKHPQGVWF LFFKGRLEF MKGDLDSSI DWYKKSWKS 
        QNLWPQFHH VCFWELLWV YCLKGNWKE ALSYCTSLL 
        EGSRWSRTI YSYQKAALM CMMREELSS TDIVVIDNL 
        MKDVPKYKQ RIAGKSLPL EKFAVKKSE RYFEQGHNL 
        ILPVIELMY VWNLFKAMK NFNVANNIF KIIDKALKD 
        LNSKTQASK YDTDNRALI LLLQGACLR QMKSPLQAL 
        NCLETVISL QKDIVEDHY LVPYAIVEL ALIEWDGGN 
        KEKAILALE DAKKNYSGY SLESRLHFR IHTALTEYK 
        AEMKNSKH
      CDS sequence
      • >TC010769 RA
        atggcttct tactccgac agtgaagat gtggacgac 
        caagactgg gatgagttc gaggatgcg gtcaacgac 
        attgacttt cctgcaccc cagggtgac atgacttta 
        gaacgtgcc gttgatgaa agtcagaag gcgataaac 
        ttttttttt aacaaccaa ttcctggag gctaaaagc 
        ttgatggag ccttatgct gctaccagc atttatcac 
        tcgctcgga cattcggta tttcttttt cttgaggct 
        attctgacg ttcgagcac aagtcgata gttaatgct 
        tcgacggct ttgaaacac gcaattcag gtgtgcaac 
        aagcaacgg aagaagaac acgtttggg gagtccttg 
        gggaagatg gtgaagcga aacaatttt gaccaatac 
        tcggaggag gaagcgcat gccgagctc tgccacgct 
        gagtgtctt ctgctcaag tcgatgctg acttttatg 
        gaagacgaa acgttgagc agttttatt aaagccggg 
        attaaaatc cggtcatgc tataattcg tacaaggac 
        tgccaacag attttactg aagagccag tgggacaaa 
        agcccgtct aaagtgcat ttcgaaagt ggggtcaaa 
        atgggtatt ggcactttt aatcttatg atatccctc 
        ttgccatcg cgagtgatc aaactatta gaatttatt 
        ggattttcc gggaacaaa caattaggt ctgaacgac 
        cttgtcgaa ggctacaaa accgacggt cttcgccag 
        attttgtgc ctgatgaca ctccttggg tacaattta 
        atcgtcctc tatgtctta agtcaccaa gaaggtgat 
        cttaagtta agccaggaa atactcgaa aagcaattg 
        aagaagcac ccgcaaggc gtttggttt ttgtttttt 
        aaaggccga ctagaattt atgaaaggt gatcttgac 
        tccagtata gactggtat aagaaatcc tggaaatcg 
        cagaatttg tggccacag ttccatcat gtttgtttc 
        tgggaattg ttgtgggtt tattgcttg aaaggaaac 
        tggaaagaa gctctctct tactgcacc tctctgctc 
        gaaggcagc cgctggtct aggaccatt tacagctat 
        cagaaagcc gcacttatg tgcatgatg agggaggag 
        ctttcgtca acagacatt gttgtgata gacaattta 
        atgaaggat gttcctaaa tataagcaa agaattgcc 
        gggaaatct ttgccgtta gaaaaattt gccgtaaaa 
        aagtcggaa cgatacttt gaacagggt cacaatcta 
        atcttgcca gtaatagaa ttaatgtac gtttggaac 
        ctgttcaaa gcgatgaag aacttcaac gttgcgaac 
        aacatattc aagataatc gacaaagcc cttaaagat 
        ttgaattca aagacacag gcttcgaaa tacgatacg 
        gacaatagg gcgcttatt ttgctgctc caaggggcg 
        tgcttgcgc caaatgaag agccctttg caggctttg 
        aactgtctg gaaactgtc atttcgttg cagaaggac 
        attgtggaa gatcattat ttggtcccg tatgccata 
        gtcgaactg gctcttatt gaatgggac gggggcaac 
        aaagagaag gcgatcctg gccctcgag gatgcaaag 
        aaaaactac tcgggctat tcattggag tcgcggctg 
        cacttccgg atccacacc gctctaact gaatacaag 
        gcggaaatg aaaaattcg aaacactaa
      mRNA sequence
      • >TC010769 RA
        acgttgttg aatgttact gttctaaaa tttatcaac 
        atgtgagta cgttttgtt aattttgta gtagaaaaa 
        gtggctttt aatacggta tgcattatt aattaattc 
        ccacgtgca ttactgcac tgcttttaa ttaacttga 
        cttaatcag tgaattaga tgaagagta atcgaatta 
        ggatacgtc ctgcctaca tattttctc aggataaat 
        tctcaaccg gcatttaca taatgtatt gccttttta 
        tcagttgcg ataaagaca tattgactg atgcaccta 
        ctcgagtga atttatcct tttgtgttt aggcgaata 
        ggagagagt gtttattat atcgaggtt agtccctac 
        tttcttcaa cttttacca aaatcttcg aagctttag 
        cagtgtata aacgatcgc gtaactaat aggattagc 
        gtaggcaat ctaactttt aacatgatt agttaagtt 
        caaacaatg actctttgt ttaccaaca gtcatggct 
        tcttactcc gacagtgaa gatgtggac gaccaagac 
        tgggatgag ttcgaggat gcggtcaac gacattgac 
        tttcctgca ccccagggt gacatgact ttagaacgt 
        gccgttgat gaaagtcag aaggcgata aactttttt 
        tttaacaac caattcctg gaggctaaa agcttgatg 
        gagccttat gctgctacc agcatttat cactcgctc 
        ggacattcg gtatttctt tttcttgag gctattctg 
        acgttcgag cacaagtcg atagttaat gcttcgacg 
        gctttgaaa cacgcaatt caggtgtgc aacaagcaa 
        cggaagaag aacacgttt ggggagtcc ttggggaag 
        atggtgaag cgaaacaat tttgaccaa tactcggag 
        gaggaagcg catgccgag ctctgccac gctgagtgt 
        cttctgctc aagtcgatg ctgactttt atggaagac 
        gaaacgttg agcagtttt attaaagcc gggattaaa 
        atccggtca tgctataat tcgtacaag gactgccaa 
        cagatttta ctgaagagc cagtgggac aaaagcccg 
        tctaaagtg catttcgaa agtggggtc aaaatgggt 
        attggcact tttaatctt atgatatcc ctcttgcca 
        tcgcgagtg atcaaacta ttagaattt attggattt 
        tccgggaac aaacaatta ggtctgaac gaccttgtc 
        gaaggctac aaaaccgac ggtcttcgc cagattttg 
        tgcctgatg acactcctt gggtacaat ttaatcgtc 
        ctctatgtc ttaagtcac caagaaggt gatcttaag 
        ttaagccag gaaatactc gaaaagcaa ttgaagaag 
        cacccgcaa ggcgtttgg tttttgttt tttaaaggc 
        cgactagaa tttatgaaa ggtgatctt gactccagt 
        atagactgg tataagaaa tcctggaaa tcgcagaat 
        ttgtggcca cagttccat catgtttgt ttctgggaa 
        ttgttgtgg gtttattgc ttgaaagga aactggaaa 
        gaagctctc tcttactgc acctctctg ctcgaaggc 
        agccgctgg tctaggacc atttacagc tatcagaaa 
        gccgcactt atgtgcatg atgagggag gagctttcg 
        tcaacagac attgttgtg atagacaat ttaatgaag 
        gatgttcct aaatataag caaagaatt gccgggaaa 
        tctttgccg ttagaaaaa tttgccgta aaaaagtcg 
        gaacgatac tttgaacag ggtcacaat ctaatcttg 
        ccagtaata gaattaatg tacgtttgg aacctgttc 
        aaagcgatg aagaacttc aacgttgcg aacaacata 
        ttcaagata atcgacaaa gcccttaaa gatttgaat 
        tcaaagaca caggcttcg aaatacgat acggacaat 
        agggcgctt attttgctg ctccaaggg gcgtgcttg 
        cgccaaatg aagagccct ttgcaggct ttgaactgt 
        ctggaaact gtcatttcg ttgcagaag gacattgtg 
        gaagatcat tatttggtc ccgtatgcc atagtcgaa 
        ctggctctt attgaatgg gacgggggc aacaaagag 
        aaggcgatc ctggccctc gaggatgca aagaaaaac 
        tactcgggc tattcattg gagtcgcgg ctgcacttc 
        cggatccac accgctcta actgaatac aaggcggaa 
        atgaaaaat tcgaaacac taaatttta acccaataa 
        cctttgaat gacagttgt gttcctgag ttagcaccc 
        ataacaaca taccatttt gtatagtcc tagcactgt 
        gtataccat atttataaa aaaggctgt gttgttata 
        ctactgtgt tgtttattt ctaaataaa tttgcattt 
        aattt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY