Status
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Gene information
General information
TC010764 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4933356..4936296
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010764
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04906

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010764 RA
      MDVTRNVIH RKQRLVDGT IVMNPRYQE PWDEEGASD 
      TCTLLEKNN FIQPDVVTL QKESLWATA IQMFIPFLL 
      AGFGMVAAS LLLDKVQHW PVYQEVSEV YILVPALLG 
      LKGNLEMTL ASRLSTHAN LGHLDTRQQ MLSLIVGNL 
      ALILCQAIV VGFLASLAA VVFGWVPSG VINVNHALL 
      LCASSIVTA FGASFILGF VMVGVILFS RYFNINPDN 
      VATPIAASL GDLTTLACL SWLASIFYH TMGSHPWLT 
      SVIIVLGLI LVPVWAWIA SRNEYTCTV LFSGWSPVI 
      AAMIISSVG GLILDFTVS QYKGVAVFQ LVINGVGGN 
      LVAVQASRI STELHSRAK PGQLPSHMQ VCISPCSAF 
      CDSRETTHA GTARMLMLM VIPGHLIFS YTISYLQAG 
      HTSFTLIFM VVYLIAALL QVLLLLYIA QVMTLSMWK 
      HNIDPDNSA IPYLTALGD LLGTLLLGV AFQFLFLIG 
      DRDSDVGD
    CDS sequence
    • >TC010764 RA
      atggacgta acacgtaat gtaattcat cgtaaacaa 
      agactggtt gatgggacg atcgtcatg aatccgcga 
      tatcaggaa ccttgggat gaggaaggg gcttcggac 
      acttgtact ttactggag aagaataat ttcattcaa 
      ccggacgtc gtgacgcta caaaaagag tcattatgg 
      gccactgcc atccaaatg ttcatcccg ttcctcctt 
      gctgggttt ggaatggtc gccgcgagc cttctcctt 
      gataaagtc cagcactgg ccagtgtac caggaagtc 
      tcggaagtg tacatcctc gtccccgcc ttactaggc 
      cttaagggc aacttagag atgacccta gcgtcccga 
      ctgtcaacc cacgcgaac ttgggtcac ctagacacc 
      cgtcaacaa atgttaagt ctgatagtt ggtaacttg 
      gccctgatt ttgtgtcag gccatcgtt gtggggttc 
      ctagcatca ctggctgcc gttgtcttc ggctgggtg 
      ccatctggt gtgataaac gtgaaccac gcacttctc 
      ctatgtgcc agtagtatt gttacggct tttggtgcg 
      agttttatc ctggggttt gtaatggtc ggggtgata 
      ctcttttcg cggtatttc aacatcaat cctgataat 
      gtggcgacg cctatcgcc gcgagtctg ggtgatctc 
      accaccttg gcatgtctt tcgtggctt gcctcgatt 
      ttttatcac accatggga agccaccca tggctgacg 
      tctgttatt attgtttta ggactgatt ttagtgcca 
      gtttgggct tggatcgcg agccgaaac gagtacacg 
      tgtactgtg ttgtttagt ggctggtcg cccgtcatc 
      gctgcgatg atcataagc agcgtcggg ggcttgatt 
      ttagatttt actgtctcg cagtataag ggtgtagct 
      gtttttcaa ctagtcatt aatggagtt ggtggaaac 
      ttagtcgct gtacaagcc agtcgcatt tcgactgaa 
      cttcatagt agggcgaag cctggccaa ttaccaagt 
      catatgcaa gtctgtatt agcccttgc tcagcattc 
      tgtgatagt cgtgagaca actcatgcc gggactgcg 
      cgcatgctc atgcttatg gtcattccg ggacatctt 
      attttttcg tacactatt agttatctc caggccggt 
      catacttct ttcactttg atcttcatg gttgtttat 
      ctgatagca gcccttctg caagtcctt ttgttgctc 
      tacatcgca caagtgatg actttgagt atgtggaag 
      cataatatc gaccccgac aattcggcc attccttac 
      ctgaccgca ctgggagac ctcctcggg actctacta 
      cttggcgta gctttccaa tttttgttc ctaatcggt 
      gaccgcgat agcgacgtt ggtgattaa
    mRNA sequence
    • >TC010764 RA
      cggccaaat caaatatcc cgctaggtg tcagttttg 
      acgttttgt gttaaaatg ttttggatt acttacatt 
      ttaatttgt gtacgtgtt aatctgatt aatcgttct 
      cttattgtt ttgcgtaac tcgcctttt ttattgtga 
      ttgggtatc aatagttgc aatgatgat tttaagggg 
      cggtgatcg tttttacgt aaaatggac gtaacacgt 
      aatgtaatt catcgtaaa caaagactg gttgatggg 
      acgatcgtc atgaatccg cgatatcag gaaccttgg 
      gatgaggaa ggggcttcg gacacttgt actttactg 
      gagaagaat aatttcatt caaccggac gtcgtgacg 
      ctacaaaaa gagtcatta tgggccact gccatccaa 
      atgttcatc ccgttcctc cttgctggg tttggaatg 
      gtcgccgcg agccttctc cttgataaa gtccagcac 
      tggccagtg taccaggaa gtctcggaa gtgtacatc 
      ctcgtcccc gccttacta ggccttaag ggcaactta 
      gagatgacc ctagcgtcc cgactgtca acccacgcg 
      aacttgggt cacctagac acccgtcaa caaatgtta 
      agtctgata gttggtaac ttggccctg attttgtgt 
      caggccatc gttgtgggg ttcctagca tcactggct 
      gccgttgtc ttcggctgg gtgccatct ggtgtgata 
      aacgtgaac cacgcactt ctcctatgt gccagtagt 
      attgttacg gcttttggt gcgagtttt atcctgggg 
      tttgtaatg gtcggggtg atactcttt tcgcggtat 
      ttcaacatc aatcctgat aatgtggcg acgcctatc 
      gccgcgagt ctgggtgat ctcaccacc ttggcatgt 
      ctttcgtgg cttgcctcg attttttat cacaccatg 
      ggaagccac ccatggctg acgtctgtt attattgtt 
      ttaggactg attttagtg ccagtttgg gcttggatc 
      gcgagccga aacgagtac acgtgtact gtgttgttt 
      agtggctgg tcgcccgtc atcgctgcg atgatcata 
      agcagcgtc gggggcttg attttagat tttactgtc 
      tcgcagtat aagggtgta gctgttttt caactagtc 
      attaatgga gttggtgga aacttagtc gctgtacaa 
      gccagtcgc atttcgact gaacttcat agtagggcg 
      aagcctggc caattacca agtcatatg caagtctgt 
      attagccct tgctcagca ttctgtgat agtcgtgag 
      acaactcat gccgggact gcgcgcatg ctcatgctt 
      atggtcatt ccgggacat cttattttt tcgtacact 
      attagttat ctccaggcc ggtcatact tctttcact 
      ttgatcttc atggttgtt tatctgata gcagccctt 
      ctgcaagtc cttttgttg ctctacatc gcacaagtg 
      atgactttg agtatgtgg aagcataat atcgacccc 
      gacaattcg gccattcct tacctgacc gcactggga 
      gacctcctc gggactcta ctacttggc gtagctttc 
      caatttttg ttcctaatc ggtgaccgc gatagcgac 
      gttggtgat taatttaga aaatatgaa ttctaaaag 
      gccacttgt gatgattaa ataactacc tttaatgta 
      cgtttgtaa taaatttcc acttgcttc attggtgct 
      attcttatg tacatacga tatatatca acattatta 
      tatacagta tcgagagaa ataaatttt ctatttcat 
      tattgtaac gcttggttt ttttggggg tttcatgta 
      tggggtgtt cgctaaaaa tgtgctaat cgcggaagc 
      accaccaac tgcaaatag taactcaag gctcttgct 
      catagcaaa ttcatgggc cattttcac gcatttaaa 
      cgacgctat gagggcgta atagcataa tgaccataa 
      taacttgtg gccattgct ggacaattg cgcagaaag 
      cagtttgga gtaagactc acaattgta aggtgcaat 
      aaaattcaa aacatagca aagtttgaa agaaaaaac 
      cacgtctta ttccaactc acaacaaac aaacttaat 
      gatcaactg acaaaaaat ctaccgtca ttaaattta 
      agtgtattg tgattataa atgtattcc cacgagaag 
      tatggattt tgagcaaaa taacaaact aatttgcac 
      tagtagaaa caaaaagag ttgcagatg accacgggg 
      tgagtagtt ttgggttta gtgagcggc tttacttaa 
      atttctcgt gctttaagt gccacattg ctttcaagt 
      tagatttag tttcaggtc tgtgtcaga agttgactt 
      actttttac tagagcaca ggaaatttg ctattatat 
      tcaataaga ttaacctag gcaataggc gctttcaaa 
      ttatgtaag tcatagcac cgtttcccg ccaatctat 
      tctcgcttg aagcatttc cctaatcag agcattttt 
      cccttcaat taaaataac tgtgtcatc ctctattac 
      aaaaacttt gaacgtctc cgaaatttc ggcttgttt 
      gatgagcag gtaattatt tcataaaaa caggcaaat 
      ttaatgtac cacgtaaaa ttgtaatgt caaggcagt 
      tttaatgaa attgtcgct cggaaaatt attttatca 
      ttttagtca aagtcgctt tgactacaa attgcttcc 
      gcttccgaa aaagatgta attgttacc tacgtaaca 
      ttgcatgtg cgccaaaac aacccacgc gccgcaaag 
      ggcaccagg tcaatgtaa ataaaaact cattaaaaa 
      a
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