| General information | |||
| TC010763 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 4868666..4928327 | |
| Sequence information | |||
| Transcripts / Proteins (5) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010763 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04905 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature pattern irregular -
Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs partially shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs partially shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC010763 RA
MGFSSLLVV LFLVAVDYG APQPARRVN VFALLSGTL RTYQRAGCD DEVVTLRCP PGTSISIEI AQYGKSAPS KSLCGPKAT QSSISKTSY NYNVSCLWP NSLQTKWEE VAQGTWGRY SLLQTVVEA CQKKRQCKF KTSPKTFGG DPCPGVRKY VEVAYKCRP YEFRSKVAC ENERIQLKC NPNSRVAVY SASYGRTEY ESIQCPQPQ GVREETCLV SYATETVMQ LCHGKRSCE LSADISTFG SPCRPESRM YLKVIYTCV PRKVLKEQY EGRLEPDEI ESDQEFATD EDDDFEPYD AGNNEYIRE SAASPPASN IDGVGKGDN LTKDSPNVN AKSPAHKSN GLDLASFQP YTTPRTNLF RPSRVPAVQ KATKDMQAD DFPDPNCTI TIYAEKTRV VGFISEWIN AYTFISQNQ ERFYLYLIV SIAAAILLC LTVLVGRLL VQRHRARRE AKFHATNVD HSLPNGFTD DISEVDADI DLTTPLPTV AIHSAVPVG SIAEVVRYP HVHSHTLRR PPQETDVPR SLSSGSNSH YYYG
- >TC010763 RB
MGFSSLLVV LFLVAVDYG APQPARRVN VFALLSGTL RTYQRAGCD DEVVTLRCP PGTSISIEI AQYGKSAPS KSLCGPKAT QSSISKTSY NYNVSCLWP NSLQYSLLQ TVVEACQKK RQCKFKTSP KTFGGDPCP GVRKYVEVA YKCRPYEFR SKVACENER IQLKCNPNS RVAVYSASY GRTEYESIQ CPQPQGVRE ETCLVSYAT ETVMQLCHG KRSCELSAD ISTFGSPCR PESRMYLKV IYTCVPRKV LKEQYEGRL EPDEIESDQ EFATDEDDD FEPYDAGNN EYIRESAAS PPASNIDGV GKGDNLTKD SPNVNAKSP AHKSNGLDL ASFQPYTTP RTNLFRPSR VPAVQKATK DMQADDFPD PNCTITIYA EKTRVVGFI SEWINAYTF ISQNQERFY LYLIVSIAA AILLCLTVL VGRLLVQRH RARREAKFH ATNVDHSLP NGFTDDISE VDADIDLTT PLPTVAIHS AVPVGSIAE VVRYPHVHS HTLRRPPQE TDVPRSLSS GSNSHYYYG
- >TC010763 RC
MGFSSLLVV LFLVAVDYG APQPARRVN VFALLSGTL RTYQRAGCD DEVVTLRCP PGTSISIEI AQYGKSAPS KSLCGPKAT QSSISKTSY NYNVSCLWP NSLQTVVEA CQKKRQCKF KTSPKTFGG DPCPGVRKY VEVAYKCRP YEFRSKVAC ENERIQLKC NPNSRVAVY SASYGRTEY ESIQCPQPQ GVREETCLV SYATETVMQ LCHGKRSCE LSADISTFG SPCRPESRM YLKVIYTCV PRKVLKEQY EGRLEPDEI ESDQEFATD EDDDFEPYD AGNNEYIRE SAASPPASN IDGVGKGDN LTKDSPNVN AKSPAHKSN GLDLASFQP YTTPRTNLF RPSRVPAVQ KATKDMQAD DFPDPNCTI TIYAEKTRV VGFISEWIN AYTFISQNQ ERFYLYLIV SIAAAILLC LTVLVGRLL VQRHRARRE AKFHATNVD HSLPNGFTD DISEVDADI DLTTPLPTV AIHSAVPVG SIAEVVRYP HVHSHTLRR PPQETDVPR SLSSGSNSH YYYG
- >TC010763 RD
MTSWIVLTV ILVGIVATN ADNLGTLRT YQRAGCDDE VVTLRCPPG TSISIEIAQ YGKSAPSKS LCGPKATQS SISKTSYNY NVSCLWPNS LQTKWEEVA QGTWGRYSL LQTVVEACQ KKRQCKFKT SPKTFGGDP CPGVRKYVE VAYKCRPYE FRSKVACEN ERIQLKCNP NSRVAVYSA SYGRTEYES IQCPQPQGV REETCLVSY ATETVMQLC HGKRSCELS ADISTFGSP CRPESRMYL KVIYTCVPR KVLKEQYEG RLEPDEIES DQEFATDED DDFEPYDAG NNEYIRESA ASPPASNID GVGKGDNLT KDSPNVNAK SPAHKSNGL DLASFQPYT TPRTNLFRP SRVPAVQKA TKDMQADDF PDPNCTITI YAEKTRVVG FISEWINAY TFISQNQER FYLYLIVSI AAAILLCLT VLVGRLLVQ RHRARREAK FHATNVDHS LPNGFTDDI SEVDADIDL TTPLPTVAI HSAVPVGSI AEVVRYPHV HSHTLRRPP QETDVPRSL SSGSNSHYY YG
- >TC010763 RE
MTSWIVLTV ILVGIVATN ADNLALLSG TLRTYQRAG CDDEVVTLR CPPGTSISI EIAQYGKSA PSKSLCGPK ATQSSISKT SYNYNVSCL WPNSLQTKW EEVAQGTWG RYSLLQTVV EACQKKRQC KFKTSPKTF GGDPCPGVR KYVEVAYKC RPYEFRSKV ACENERIQL KCNPNSRVA VYSASYGRT EYESIQCPQ PQGVREETC LVSYATETV MQLCHGKRS CELSADIST FGSPCRPES RMYLKVIYT CVPRKVLKE QYEGRLEPD EIESDQEFA TDEDDDFEP YDAGNNEYI RESAASPPA SNIDGVGKG DNLTKDSPN VNAKSPAHK SNGLDLASF QPYTTPRTN LFRPSRVPA VQKATKDMQ ADDFPDPNC TITIYAEKT RVVGFISEW INAYTFISQ NQERFYLYL IVSIAAAIL LCLTVLVGR LLVQRHRAR REAKFHATN VDHSLPNGF TDDISEVDA DIDLTTPLP TVAIHSAVP VGSIAEVVR YPHVHSHTL RRPPQETDV PRSLSSGSN SHYYYG
- >TC010763 RA
atgggattt tcatccctt ttggtggtc ctgttcctg gttgctgta gactacggg gcgccgcag cctgcacgt agagtaaac gtgttcgct ttgttatca ggaactctg aggacgtac cagcgagcc ggttgtgac gatgaagtt gtgactctc cgatgcccc cctggaacg agcatctcg atcgaaatc gcccagtat ggaaaatca gctccgtcc aaatcgttg tgcggccct aaagcaacc caatcttcg atttccaaa acatcctac aattacaac gtttcctgt ctctggccc aattcatta cagaccaag tgggaggaa gtagctcaa ggaacatgg gggagatac tcgttgttg cagactgtg gtggaggcg tgccagaag aagcgtcag tgcaagttt aaaacgtcc ccgaagaca ttcggcggg gatccctgt ccgggtgtt cgcaaatat gtcgaagtg gcttataaa tgtagaccg tatgagttt cgaagtaaa gtggcctgc gagaatgaa cgaattcaa ttgaagtgc aatcccaac tcaagggtt gccgtatac agcgccagc tacgggagg acggaatac gaaagcatt caatgtccc cagccccaa ggcgtgcgc gaggagacg tgcttggtg agttacgct accgagact gtgatgcag ctttgccac ggcaagcgg agttgtgaa ttatcagcc gacataagt acttttggc agcccctgt cggccggag tcccgcatg tatctaaaa gtgatctac acctgcgtt ccccggaaa gtactcaag gagcagtac gaggggcgc ctcgaacct gacgaaatt gaatccgac caagagttt gccaccgac gaggacgac gacttcgag ccgtacgat gccggcaat aacgagtat atcagagag tcggctgct tctccgccc gcctccaac atcgacggc gtcgggaaa ggggataat ctaaccaag gactcgccc aacgttaac gctaagagt cctgcacac aagtcgaac ggacttgat cttgcgtcc tttcagcct tacacaact cctcgtact aacctgttc cgcccatcc cgagttcct gccgtgcag aaagctact aaagacatg caagccgac gatttccca gatcccaac tgcacgatc acgatctat gcagagaaa actagagtc gtcggattc atttccgaa tggataaat gcgtacacg ttcatttca cagaaccaa gagcggttc tacctgtac ctgattgtg agcatcgcg gcggccatc ctcctgtgt ctgacagtg ctggtgggc cggctcctg gtgcagcgg caccgggct cggcgcgag gccaagttc cacgccacc aacgtcgac cattctctg cccaacggg ttcacggac gacatcagc gaggtggac gcggacata gacttgacg acgccgctg ccgacggtg gccatccac tcggccgtg cccgtgggc agcatcgcc gaggtggtg cggtatccc cacgtccac tcgcacacg ctgcggagg ccgccccag gagacggac gtcccccgg agtctcagc agcggctcg aacagtcac tactactac gggtga
- >TC010763 RB
atgggattt tcatccctt ttggtggtc ctgttcctg gttgctgta gactacggg gcgccgcag cctgcacgt agagtaaac gtgttcgct ttgttatca ggaactctg aggacgtac cagcgagcc ggttgtgac gatgaagtt gtgactctc cgatgcccc cctggaacg agcatctcg atcgaaatc gcccagtat ggaaaatca gctccgtcc aaatcgttg tgcggccct aaagcaacc caatcttcg atttccaaa acatcctac aattacaac gtttcctgt ctctggccc aattcatta cagtactcg ttgttgcag actgtggtg gaggcgtgc cagaagaag cgtcagtgc aagtttaaa acgtccccg aagacattc ggcggggat ccctgtccg ggtgttcgc aaatatgtc gaagtggct tataaatgt agaccgtat gagtttcga agtaaagtg gcctgcgag aatgaacga attcaattg aagtgcaat cccaactca agggttgcc gtatacagc gccagctac gggaggacg gaatacgaa agcattcaa tgtccccag ccccaaggc gtgcgcgag gagacgtgc ttggtgagt tacgctacc gagactgtg atgcagctt tgccacggc aagcggagt tgtgaatta tcagccgac ataagtact tttggcagc ccctgtcgg ccggagtcc cgcatgtat ctaaaagtg atctacacc tgcgttccc cggaaagta ctcaaggag cagtacgag gggcgcctc gaacctgac gaaattgaa tccgaccaa gagtttgcc accgacgag gacgacgac ttcgagccg tacgatgcc ggcaataac gagtatatc agagagtcg gctgcttct ccgcccgcc tccaacatc gacggcgtc gggaaaggg gataatcta accaaggac tcgcccaac gttaacgct aagagtcct gcacacaag tcgaacgga cttgatctt gcgtccttt cagccttac acaactcct cgtactaac ctgttccgc ccatcccga gttcctgcc gtgcagaaa gctactaaa gacatgcaa gccgacgat ttcccagat cccaactgc acgatcacg atctatgca gagaaaact agagtcgtc ggattcatt tccgaatgg ataaatgcg tacacgttc atttcacag aaccaagag cggttctac ctgtacctg attgtgagc atcgcggcg gccatcctc ctgtgtctg acagtgctg gtgggccgg ctcctggtg cagcggcac cgggctcgg cgcgaggcc aagttccac gccaccaac gtcgaccat tctctgccc aacgggttc acggacgac atcagcgag gtggacgcg gacatagac ttgacgacg ccgctgccg acggtggcc atccactcg gccgtgccc gtgggcagc atcgccgag gtggtgcgg tatccccac gtccactcg cacacgctg cggaggccg ccccaggag acggacgtc ccccggagt ctcagcagc ggctcgaac agtcactac tactacggg tga
- >TC010763 RC
atgggattt tcatccctt ttggtggtc ctgttcctg gttgctgta gactacggg gcgccgcag cctgcacgt agagtaaac gtgttcgct ttgttatca ggaactctg aggacgtac cagcgagcc ggttgtgac gatgaagtt gtgactctc cgatgcccc cctggaacg agcatctcg atcgaaatc gcccagtat ggaaaatca gctccgtcc aaatcgttg tgcggccct aaagcaacc caatcttcg atttccaaa acatcctac aattacaac gtttcctgt ctctggccc aattcatta cagactgtg gtggaggcg tgccagaag aagcgtcag tgcaagttt aaaacgtcc ccgaagaca ttcggcggg gatccctgt ccgggtgtt cgcaaatat gtcgaagtg gcttataaa tgtagaccg tatgagttt cgaagtaaa gtggcctgc gagaatgaa cgaattcaa ttgaagtgc aatcccaac tcaagggtt gccgtatac agcgccagc tacgggagg acggaatac gaaagcatt caatgtccc cagccccaa ggcgtgcgc gaggagacg tgcttggtg agttacgct accgagact gtgatgcag ctttgccac ggcaagcgg agttgtgaa ttatcagcc gacataagt acttttggc agcccctgt cggccggag tcccgcatg tatctaaaa gtgatctac acctgcgtt ccccggaaa gtactcaag gagcagtac gaggggcgc ctcgaacct gacgaaatt gaatccgac caagagttt gccaccgac gaggacgac gacttcgag ccgtacgat gccggcaat aacgagtat atcagagag tcggctgct tctccgccc gcctccaac atcgacggc gtcgggaaa ggggataat ctaaccaag gactcgccc aacgttaac gctaagagt cctgcacac aagtcgaac ggacttgat cttgcgtcc tttcagcct tacacaact cctcgtact aacctgttc cgcccatcc cgagttcct gccgtgcag aaagctact aaagacatg caagccgac gatttccca gatcccaac tgcacgatc acgatctat gcagagaaa actagagtc gtcggattc atttccgaa tggataaat gcgtacacg ttcatttca cagaaccaa gagcggttc tacctgtac ctgattgtg agcatcgcg gcggccatc ctcctgtgt ctgacagtg ctggtgggc cggctcctg gtgcagcgg caccgggct cggcgcgag gccaagttc cacgccacc aacgtcgac cattctctg cccaacggg ttcacggac gacatcagc gaggtggac gcggacata gacttgacg acgccgctg ccgacggtg gccatccac tcggccgtg cccgtgggc agcatcgcc gaggtggtg cggtatccc cacgtccac tcgcacacg ctgcggagg ccgccccag gagacggac gtcccccgg agtctcagc agcggctcg aacagtcac tactactac gggtga
- >TC010763 RD
atgacttcc tggatcgtc cttacggtt atcctagtc ggaatcgtc gccactaat gcggataat ttaggaact ctgaggacg taccagcga gccggttgt gacgatgaa gttgtgact ctccgatgc ccccctgga acgagcatc tcgatcgaa atcgcccag tatggaaaa tcagctccg tccaaatcg ttgtgcggc cctaaagca acccaatct tcgatttcc aaaacatcc tacaattac aacgtttcc tgtctctgg cccaattca ttacagacc aagtgggag gaagtagct caaggaaca tgggggaga tactcgttg ttgcagact gtggtggag gcgtgccag aagaagcgt cagtgcaag tttaaaacg tccccgaag acattcggc ggggatccc tgtccgggt gttcgcaaa tatgtcgaa gtggcttat aaatgtaga ccgtatgag tttcgaagt aaagtggcc tgcgagaat gaacgaatt caattgaag tgcaatccc aactcaagg gttgccgta tacagcgcc agctacggg aggacggaa tacgaaagc attcaatgt ccccagccc caaggcgtg cgcgaggag acgtgcttg gtgagttac gctaccgag actgtgatg cagctttgc cacggcaag cggagttgt gaattatca gccgacata agtactttt ggcagcccc tgtcggccg gagtcccgc atgtatcta aaagtgatc tacacctgc gttccccgg aaagtactc aaggagcag tacgagggg cgcctcgaa cctgacgaa attgaatcc gaccaagag tttgccacc gacgaggac gacgacttc gagccgtac gatgccggc aataacgag tatatcaga gagtcggct gcttctccg cccgcctcc aacatcgac ggcgtcggg aaaggggat aatctaacc aaggactcg cccaacgtt aacgctaag agtcctgca cacaagtcg aacggactt gatcttgcg tcctttcag ccttacaca actcctcgt actaacctg ttccgccca tcccgagtt cctgccgtg cagaaagct actaaagac atgcaagcc gacgatttc ccagatccc aactgcacg atcacgatc tatgcagag aaaactaga gtcgtcgga ttcatttcc gaatggata aatgcgtac acgttcatt tcacagaac caagagcgg ttctacctg tacctgatt gtgagcatc gcggcggcc atcctcctg tgtctgaca gtgctggtg ggccggctc ctggtgcag cggcaccgg gctcggcgc gaggccaag ttccacgcc accaacgtc gaccattct ctgcccaac gggttcacg gacgacatc agcgaggtg gacgcggac atagacttg acgacgccg ctgccgacg gtggccatc cactcggcc gtgcccgtg ggcagcatc gccgaggtg gtgcggtat ccccacgtc cactcgcac acgctgcgg aggccgccc caggagacg gacgtcccc cggagtctc agcagcggc tcgaacagt cactactac tacgggtga
- >TC010763 RE
atgacttcc tggatcgtc cttacggtt atcctagtc ggaatcgtc gccactaat gcggataat ttagctttg ttatcagga actctgagg acgtaccag cgagccggt tgtgacgat gaagttgtg actctccga tgcccccct ggaacgagc atctcgatc gaaatcgcc cagtatgga aaatcagct ccgtccaaa tcgttgtgc ggccctaaa gcaacccaa tcttcgatt tccaaaaca tcctacaat tacaacgtt tcctgtctc tggcccaat tcattacag accaagtgg gaggaagta gctcaagga acatggggg agatactcg ttgttgcag actgtggtg gaggcgtgc cagaagaag cgtcagtgc aagtttaaa acgtccccg aagacattc ggcggggat ccctgtccg ggtgttcgc aaatatgtc gaagtggct tataaatgt agaccgtat gagtttcga agtaaagtg gcctgcgag aatgaacga attcaattg aagtgcaat cccaactca agggttgcc gtatacagc gccagctac gggaggacg gaatacgaa agcattcaa tgtccccag ccccaaggc gtgcgcgag gagacgtgc ttggtgagt tacgctacc gagactgtg atgcagctt tgccacggc aagcggagt tgtgaatta tcagccgac ataagtact tttggcagc ccctgtcgg ccggagtcc cgcatgtat ctaaaagtg atctacacc tgcgttccc cggaaagta ctcaaggag cagtacgag gggcgcctc gaacctgac gaaattgaa tccgaccaa gagtttgcc accgacgag gacgacgac ttcgagccg tacgatgcc ggcaataac gagtatatc agagagtcg gctgcttct ccgcccgcc tccaacatc gacggcgtc gggaaaggg gataatcta accaaggac tcgcccaac gttaacgct aagagtcct gcacacaag tcgaacgga cttgatctt gcgtccttt cagccttac acaactcct cgtactaac ctgttccgc ccatcccga gttcctgcc gtgcagaaa gctactaaa gacatgcaa gccgacgat ttcccagat cccaactgc acgatcacg atctatgca gagaaaact agagtcgtc ggattcatt tccgaatgg ataaatgcg tacacgttc atttcacag aaccaagag cggttctac ctgtacctg attgtgagc atcgcggcg gccatcctc ctgtgtctg acagtgctg gtgggccgg ctcctggtg cagcggcac cgggctcgg cgcgaggcc aagttccac gccaccaac gtcgaccat tctctgccc aacgggttc acggacgac atcagcgag gtggacgcg gacatagac ttgacgacg ccgctgccg acggtggcc atccactcg gccgtgccc gtgggcagc atcgccgag gtggtgcgg tatccccac gtccactcg cacacgctg cggaggccg ccccaggag acggacgtc ccccggagt ctcagcagc ggctcgaac agtcactac tactacggg tga
- >TC010763 RA
ccagtccgt gcgcgatgc gctcctaac tactagtgc tagtgctag tcatgtgtc agcatggga ttttcatcc cttttggtg gtcctgttc ctggttgct gtagactac ggggcgccg cagcctgca cgtagagta aacgtgttc gctttgtta tcaggaact ctgaggacg taccagcga gccggttgt gacgatgaa gttgtgact ctccgatgc ccccctgga acgagcatc tcgatcgaa atcgcccag tatggaaaa tcagctccg tccaaatcg ttgtgcggc cctaaagca acccaatct tcgatttcc aaaacatcc tacaattac aacgtttcc tgtctctgg cccaattca ttacagacc aagtgggag gaagtagct caaggaaca tgggggaga tactcgttg ttgcagact gtggtggag gcgtgccag aagaagcgt cagtgcaag tttaaaacg tccccgaag acattcggc ggggatccc tgtccgggt gttcgcaaa tatgtcgaa gtggcttat aaatgtaga ccgtatgag tttcgaagt aaagtggcc tgcgagaat gaacgaatt caattgaag tgcaatccc aactcaagg gttgccgta tacagcgcc agctacggg aggacggaa tacgaaagc attcaatgt ccccagccc caaggcgtg cgcgaggag acgtgcttg gtgagttac gctaccgag actgtgatg cagctttgc cacggcaag cggagttgt gaattatca gccgacata agtactttt ggcagcccc tgtcggccg gagtcccgc atgtatcta aaagtgatc tacacctgc gttccccgg aaagtactc aaggagcag tacgagggg cgcctcgaa cctgacgaa attgaatcc gaccaagag tttgccacc gacgaggac gacgacttc gagccgtac gatgccggc aataacgag tatatcaga gagtcggct gcttctccg cccgcctcc aacatcgac ggcgtcggg aaaggggat aatctaacc aaggactcg cccaacgtt aacgctaag agtcctgca cacaagtcg aacggactt gatcttgcg tcctttcag ccttacaca actcctcgt actaacctg ttccgccca tcccgagtt cctgccgtg cagaaagct actaaagac atgcaagcc gacgatttc ccagatccc aactgcacg atcacgatc tatgcagag aaaactaga gtcgtcgga ttcatttcc gaatggata aatgcgtac acgttcatt tcacagaac caagagcgg ttctacctg tacctgatt gtgagcatc gcggcggcc atcctcctg tgtctgaca gtgctggtg ggccggctc ctggtgcag cggcaccgg gctcggcgc gaggccaag ttccacgcc accaacgtc gaccattct ctgcccaac gggttcacg gacgacatc agcgaggtg gacgcggac atagacttg acgacgccg ctgccgacg gtggccatc cactcggcc gtgcccgtg ggcagcatc gccgaggtg gtgcggtat ccccacgtc cactcgcac acgctgcgg aggccgccc caggagacg gacgtcccc cggagtctc agcagcggc tcgaacagt cactactac tacgggtga ccagaatgc ccggggcgc ggatcggcg gtgccgacc tcccccacg tcctctgcg tgcccgggc caacgtacc tattgcttc tagtgacgc tcagtgccg cgaccagtg acccagacc aaagaaact cttctcctc aagactttg tgaattatt tcgttatac cgctcggaa gaattgtaa ttacaagcg ccatttctt cgatctcgc ggtgccagg cactctaga caggaaact gcctatgcc gtgattccc gcgattaaa taaatgctc ctcctgttg aaccttatc cccagtaga tatgttgtt cttaattgt tattgtata ctgttcgaa ggtgggttg gaaaaaaac aaggcttgt aattataat ttaaaacac tttgttaat atcttaaat gactaatta atatcaaaa attgtttat atactaaac tggatcttt atataaaaa agaaaacag agtacgtat a
- >TC010763 RB
ccagtccgt gcgcgatgc gctcctaac tactagtgc tagtgctag tcatgtgtc agcatggga ttttcatcc cttttggtg gtcctgttc ctggttgct gtagactac ggggcgccg cagcctgca cgtagagta aacgtgttc gctttgtta tcaggaact ctgaggacg taccagcga gccggttgt gacgatgaa gttgtgact ctccgatgc ccccctgga acgagcatc tcgatcgaa atcgcccag tatggaaaa tcagctccg tccaaatcg ttgtgcggc cctaaagca acccaatct tcgatttcc aaaacatcc tacaattac aacgtttcc tgtctctgg cccaattca ttacagtac tcgttgttg cagactgtg gtggaggcg tgccagaag aagcgtcag tgcaagttt aaaacgtcc ccgaagaca ttcggcggg gatccctgt ccgggtgtt cgcaaatat gtcgaagtg gcttataaa tgtagaccg tatgagttt cgaagtaaa gtggcctgc gagaatgaa cgaattcaa ttgaagtgc aatcccaac tcaagggtt gccgtatac agcgccagc tacgggagg acggaatac gaaagcatt caatgtccc cagccccaa ggcgtgcgc gaggagacg tgcttggtg agttacgct accgagact gtgatgcag ctttgccac ggcaagcgg agttgtgaa ttatcagcc gacataagt acttttggc agcccctgt cggccggag tcccgcatg tatctaaaa gtgatctac acctgcgtt ccccggaaa gtactcaag gagcagtac gaggggcgc ctcgaacct gacgaaatt gaatccgac caagagttt gccaccgac gaggacgac gacttcgag ccgtacgat gccggcaat aacgagtat atcagagag tcggctgct tctccgccc gcctccaac atcgacggc gtcgggaaa ggggataat ctaaccaag gactcgccc aacgttaac gctaagagt cctgcacac aagtcgaac ggacttgat cttgcgtcc tttcagcct tacacaact cctcgtact aacctgttc cgcccatcc cgagttcct gccgtgcag aaagctact aaagacatg caagccgac gatttccca gatcccaac tgcacgatc acgatctat gcagagaaa actagagtc gtcggattc atttccgaa tggataaat gcgtacacg ttcatttca cagaaccaa gagcggttc tacctgtac ctgattgtg agcatcgcg gcggccatc ctcctgtgt ctgacagtg ctggtgggc cggctcctg gtgcagcgg caccgggct cggcgcgag gccaagttc cacgccacc aacgtcgac cattctctg cccaacggg ttcacggac gacatcagc gaggtggac gcggacata gacttgacg acgccgctg ccgacggtg gccatccac tcggccgtg cccgtgggc agcatcgcc gaggtggtg cggtatccc cacgtccac tcgcacacg ctgcggagg ccgccccag gagacggac gtcccccgg agtctcagc agcggctcg aacagtcac tactactac gggtgacca gaatgcccg gggcgcgga tcggcggtg ccgacctcc cccacgtcc tctgcgtgc ccgggccaa cgtacctat tgcttctag tgacgctca gtgccgcga ccagtgacc cagaccaaa gaaactctt ctcctcaag actttgtga attatttcg ttataccgc tcggaagaa ttgtaatta caagcgcca tttcttcga tctcgcggt gccaggcac tctagacag gaaactgcc tatgccgtg attcccgcg attaaataa atgctcctc ctgttgaac cttatcccc agtagatat gttgttctt aattgttat tgtatactg ttcgaaggt gggttggaa aaaaacaag gcttgtaat tataattta aaacacttt gttaatatc ttaaatgac taattaata tcaaaaatt gtttatata ctaaactgg atctttata taaaaaaga aaacagagt acgtata
- >TC010763 RC
ccagtccgt gcgcgatgc gctcctaac tactagtgc tagtgctag tcatgtgtc agcatggga ttttcatcc cttttggtg gtcctgttc ctggttgct gtagactac ggggcgccg cagcctgca cgtagagta aacgtgttc gctttgtta tcaggaact ctgaggacg taccagcga gccggttgt gacgatgaa gttgtgact ctccgatgc ccccctgga acgagcatc tcgatcgaa atcgcccag tatggaaaa tcagctccg tccaaatcg ttgtgcggc cctaaagca acccaatct tcgatttcc aaaacatcc tacaattac aacgtttcc tgtctctgg cccaattca ttacagact gtggtggag gcgtgccag aagaagcgt cagtgcaag tttaaaacg tccccgaag acattcggc ggggatccc tgtccgggt gttcgcaaa tatgtcgaa gtggcttat aaatgtaga ccgtatgag tttcgaagt aaagtggcc tgcgagaat gaacgaatt caattgaag tgcaatccc aactcaagg gttgccgta tacagcgcc agctacggg aggacggaa tacgaaagc attcaatgt ccccagccc caaggcgtg cgcgaggag acgtgcttg gtgagttac gctaccgag actgtgatg cagctttgc cacggcaag cggagttgt gaattatca gccgacata agtactttt ggcagcccc tgtcggccg gagtcccgc atgtatcta aaagtgatc tacacctgc gttccccgg aaagtactc aaggagcag tacgagggg cgcctcgaa cctgacgaa attgaatcc gaccaagag tttgccacc gacgaggac gacgacttc gagccgtac gatgccggc aataacgag tatatcaga gagtcggct gcttctccg cccgcctcc aacatcgac ggcgtcggg aaaggggat aatctaacc aaggactcg cccaacgtt aacgctaag agtcctgca cacaagtcg aacggactt gatcttgcg tcctttcag ccttacaca actcctcgt actaacctg ttccgccca tcccgagtt cctgccgtg cagaaagct actaaagac atgcaagcc gacgatttc ccagatccc aactgcacg atcacgatc tatgcagag aaaactaga gtcgtcgga ttcatttcc gaatggata aatgcgtac acgttcatt tcacagaac caagagcgg ttctacctg tacctgatt gtgagcatc gcggcggcc atcctcctg tgtctgaca gtgctggtg ggccggctc ctggtgcag cggcaccgg gctcggcgc gaggccaag ttccacgcc accaacgtc gaccattct ctgcccaac gggttcacg gacgacatc agcgaggtg gacgcggac atagacttg acgacgccg ctgccgacg gtggccatc cactcggcc gtgcccgtg ggcagcatc gccgaggtg gtgcggtat ccccacgtc cactcgcac acgctgcgg aggccgccc caggagacg gacgtcccc cggagtctc agcagcggc tcgaacagt cactactac tacgggtga ccagaatgc ccggggcgc ggatcggcg gtgccgacc tcccccacg tcctctgcg tgcccgggc caacgtacc tattgcttc tagtgacgc tcagtgccg cgaccagtg acccagacc aaagaaact cttctcctc aagactttg tgaattatt tcgttatac cgctcggaa gaattgtaa ttacaagcg ccatttctt cgatctcgc ggtgccagg cactctaga caggaaact gcctatgcc gtgattccc gcgattaaa taaatgctc ctcctgttg aaccttatc cccagtaga tatgttgtt cttaattgt tattgtata ctgttcgaa ggtgggttg gaaaaaaac aaggcttgt aattataat ttaaaacac tttgttaat atcttaaat gactaatta atatcaaaa attgtttat atactaaac tggatcttt atataaaaa agaaaacag agtacgtat a
- >TC010763 RD
tgggggagt aaaccgtat tcgtccgtt tccgtgcgt tgtcgtcgt ttggttggt ccattcgta acatggact tggttttac agaatcagt ttagtactg atacaaccg ccgttagag ggagttcgt gaacttgac actggagga gccaagaaa cacgctcgc ggtatatct ttctcccat ttgaaaatg tgaaaaatt tgcgcggaa cggaaattc tcgatttaa attcacctt agcggttat taaaatggt attgttagt gtttcgtga tgcctaagt gatttaaaa tgacttcct ggatcgtcc ttacggtta tcctagtcg gaatcgtcg ccactaatg cggataatt taggaactc tgaggacgt accagcgag ccggttgtg acgatgaag ttgtgactc tccgatgcc cccctggaa cgagcatct cgatcgaaa tcgcccagt atggaaaat cagctccgt ccaaatcgt tgtgcggcc ctaaagcaa cccaatctt cgatttcca aaacatcct acaattaca acgtttcct gtctctggc ccaattcat tacagacca agtgggagg aagtagctc aaggaacat gggggagat actcgttgt tgcagactg tggtggagg cgtgccaga agaagcgtc agtgcaagt ttaaaacgt ccccgaaga cattcggcg gggatccct gtccgggtg ttcgcaaat atgtcgaag tggcttata aatgtagac cgtatgagt ttcgaagta aagtggcct gcgagaatg aacgaattc aattgaagt gcaatccca actcaaggg ttgccgtat acagcgcca gctacggga ggacggaat acgaaagca ttcaatgtc cccagcccc aaggcgtgc gcgaggaga cgtgcttgg tgagttacg ctaccgaga ctgtgatgc agctttgcc acggcaagc ggagttgtg aattatcag ccgacataa gtacttttg gcagcccct gtcggccgg agtcccgca tgtatctaa aagtgatct acacctgcg ttccccgga aagtactca aggagcagt acgaggggc gcctcgaac ctgacgaaa ttgaatccg accaagagt ttgccaccg acgaggacg acgacttcg agccgtacg atgccggca ataacgagt atatcagag agtcggctg cttctccgc ccgcctcca acatcgacg gcgtcggga aaggggata atctaacca aggactcgc ccaacgtta acgctaaga gtcctgcac acaagtcga acggacttg atcttgcgt cctttcagc cttacacaa ctcctcgta ctaacctgt tccgcccat cccgagttc ctgccgtgc agaaagcta ctaaagaca tgcaagccg acgatttcc cagatccca actgcacga tcacgatct atgcagaga aaactagag tcgtcggat tcatttccg aatggataa atgcgtaca cgttcattt cacagaacc aagagcggt tctacctgt acctgattg tgagcatcg cggcggcca tcctcctgt gtctgacag tgctggtgg gccggctcc tggtgcagc ggcaccggg ctcggcgcg aggccaagt tccacgcca ccaacgtcg accattctc tgcccaacg ggttcacgg acgacatca gcgaggtgg acgcggaca tagacttga cgacgccgc tgccgacgg tggccatcc actcggccg tgcccgtgg gcagcatcg ccgaggtgg tgcggtatc cccacgtcc actcgcaca cgctgcgga ggccgcccc aggagacgg acgtccccc ggagtctca gcagcggct cgaacagtc actactact acgggtgac cagaatgcc cggggcgcg gatcggcgg tgccgacct cccccacgt cctctgcgt gcccgggcc aacgtacct attgcttct agtgacgct cagtgccgc gaccagtga cccagacca aagaaactc ttctcctca agactttgt gaattattt cgttatacc gctcggaag aattgtaat tacaagcgc catttcttc gatctcgcg gtgccaggc actctagac aggaaactg cctatgccg tgattcccg cgattaaat aaatgctcc tcctgttga accttatcc ccagtagat atgttgttc ttaattgtt attgtatac tgttcgaag gtgggttgg aaaaaaaca aggcttgta attataatt taaaacact ttgttaata tcttaaatg actaattaa tatcaaaaa ttgtttata tactaaact ggatcttta tataaaaaa gaaaacaga gtacgtata
- >TC010763 RE
tgggggagt aaaccgtat tcgtccgtt tccgtgcgt tgtcgtcgt ttggttggt ccattcgta acatggact tggttttac agaatcagt ttagtactg atacaaccg ccgttagag ggagttcgt gaacttgac actggagga gccaagaaa cacgctcgc ggtatatct ttctcccat ttgaaaatg tgaaaaatt tgcgcggaa cggaaattc tcgatttaa attcacctt agcggttat taaaatggt attgttagt gtttcgtga tgcctaagt gatttaaaa tgacttcct ggatcgtcc ttacggtta tcctagtcg gaatcgtcg ccactaatg cggataatt tagctttgt tatcaggaa ctctgagga cgtaccagc gagccggtt gtgacgatg aagttgtga ctctccgat gcccccctg gaacgagca tctcgatcg aaatcgccc agtatggaa aatcagctc cgtccaaat cgttgtgcg gccctaaag caacccaat cttcgattt ccaaaacat cctacaatt acaacgttt cctgtctct ggcccaatt cattacaga ccaagtggg aggaagtag ctcaaggaa catggggga gatactcgt tgttgcaga ctgtggtgg aggcgtgcc agaagaagc gtcagtgca agtttaaaa cgtccccga agacattcg gcggggatc cctgtccgg gtgttcgca aatatgtcg aagtggctt ataaatgta gaccgtatg agtttcgaa gtaaagtgg cctgcgaga atgaacgaa ttcaattga agtgcaatc ccaactcaa gggttgccg tatacagcg ccagctacg ggaggacgg aatacgaaa gcattcaat gtccccagc cccaaggcg tgcgcgagg agacgtgct tggtgagtt acgctaccg agactgtga tgcagcttt gccacggca agcggagtt gtgaattat cagccgaca taagtactt ttggcagcc cctgtcggc cggagtccc gcatgtatc taaaagtga tctacacct gcgttcccc ggaaagtac tcaaggagc agtacgagg ggcgcctcg aacctgacg aaattgaat ccgaccaag agtttgcca ccgacgagg acgacgact tcgagccgt acgatgccg gcaataacg agtatatca gagagtcgg ctgcttctc cgcccgcct ccaacatcg acggcgtcg ggaaagggg ataatctaa ccaaggact cgcccaacg ttaacgcta agagtcctg cacacaagt cgaacggac ttgatcttg cgtcctttc agccttaca caactcctc gtactaacc tgttccgcc catcccgag ttcctgccg tgcagaaag ctactaaag acatgcaag ccgacgatt tcccagatc ccaactgca cgatcacga tctatgcag agaaaacta gagtcgtcg gattcattt ccgaatgga taaatgcgt acacgttca tttcacaga accaagagc ggttctacc tgtacctga ttgtgagca tcgcggcgg ccatcctcc tgtgtctga cagtgctgg tgggccggc tcctggtgc agcggcacc gggctcggc gcgaggcca agttccacg ccaccaacg tcgaccatt ctctgccca acgggttca cggacgaca tcagcgagg tggacgcgg acatagact tgacgacgc cgctgccga cggtggcca tccactcgg ccgtgcccg tgggcagca tcgccgagg tggtgcggt atccccacg tccactcgc acacgctgc ggaggccgc cccaggaga cggacgtcc cccggagtc tcagcagcg gctcgaaca gtcactact actacgggt gaccagaat gcccggggc gcggatcgg cggtgccga cctccccca cgtcctctg cgtgcccgg gccaacgta cctattgct tctagtgac gctcagtgc cgcgaccag tgacccaga ccaaagaaa ctcttctcc tcaagactt tgtgaatta tttcgttat accgctcgg aagaattgt aattacaag cgccatttc ttcgatctc gcggtgcca ggcactcta gacaggaaa ctgcctatg ccgtgattc ccgcgatta aataaatgc tcctcctgt tgaacctta tccccagta gatatgttg ttcttaatt gttattgta tactgttcg aaggtgggt tggaaaaaa acaaggctt gtaattata atttaaaac actttgtta atatcttaa atgactaat taatatcaa aaattgttt atatactaa actggatct ttatataaa aaagaaaac agagtacgt ata
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