Status
Please wait ...
Gene information
General information
TC031600 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450519.1: 42..2229
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031600
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04903

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031600 RA
        MSHRLAPPK ATSTKPKPT LKPPATKQK KSGESVADL 
        LKHQTPKPP PQTFATATT SSNMQNMQI LSRSRSPIN 
        KTLDKPVSP ISSRESSMS VSESEYKYT VRNLPQHIA 
        TQKSFYSFL ITTINLRSI LTLKVNFNR TALLITSAP 
        ITPAIINQF RTASGDANI TVDPINKKS LNTPFPKVP 
        TFSVVIRSV EHDITEADI MNQNPILKI NRLHRIISK 
        KTNRPTPLI RVISDDKPT IDHLLTQGI TIFSRVYDC 
        EVSHPPPPT PLQCSKCFQ LGHGPNDCP NKPICPKCP 
        ESHHPSKCT VITAKCPFC QGSHPAWSR QCPVIKETP 
        ITENTPFVP TKIIDPPSE FADRDIAED PDSPHSDPN 
        PTQTLLRQV IAFFAKILF DIFPLQKPK MQQIIEQAS 
        LHAFEIITK ISHSGHRIR FSFN
      CDS sequence
      • >TC031600 RA
        atgtctcat cgtctcgct ccccccaaa gcgacttcg 
        accaagccg aagcccacg ctgaagccc cctgcgacc 
        aagcagaaa aaatccggt gagtctgtt gccgattta 
        cttaaacat caaacacca aaacctcct ccccaaacc 
        ttcgctacc gccactacc tcctccaac atgcaaaat 
        atgcaaatc ctttcccgc tcccgctct cccatcaac 
        aaaaccctt gacaaaccc gtatcccca atctcctct 
        cgcgaatcc agcatgtct gtttccgag tcagaatac 
        aaatatacc gtccgaaac ctgccccaa cacattgct 
        acccaaaaa tcattctat tctttttta atcacaaca 
        attaacctc agatcaatc ttaaccctg aaagtcaat 
        tttaaccga accgcgctc ctaattact tctgccccc 
        attacccca gccatcatc aatcaattc cgcaccgca 
        tccggtgat gccaacata actgtcgac cctattaac 
        aaaaaatcc ctaaatacc ccattccca aaagtccct 
        acattttcc gttgttatc cgcagcgtc gaacacgac 
        attaccgaa gccgatatc atgaaccaa aacccaatc 
        ctcaaaatt aaccgcctc cacagaatt atctccaaa 
        aaaaccaat cgtcccacc ccattaatc cgcgtcatc 
        tccgacgat aaacccaca atagatcac cttttaacc 
        caaggcatc accatcttt tcccgcgtc tacgactgc 
        gaagtctcc catccacct cccccaact ccactgcaa 
        tgctccaaa tgcttccaa ctgggtcat ggccccaat 
        gattgcccc aacaaaccc atttgcccc aaatgcccc 
        gaatctcat cacccatcc aaatgcacc gtcattaca 
        gccaaatgc cccttctgc caaggctcc caccctgca 
        tggtcccgt caatgtcca gttatcaaa gaaaccccc 
        attaccgaa aatacccca ttcgtccca acaaaaatc 
        attgacccc ccttccgag tttgccgac cgagacata 
        gccgaagac cccgacagc ccccactct gaccccaac 
        ccaacccaa actctcctc cgacaagtc atcgctttc 
        tttgcaaaa attcttttc gacattttt ccccttcag 
        aaacccaaa atgcaacaa attattgaa caagcctcc 
        ttacatgcc tttgaaatt atcaccaaa atttcccac 
        tccggccac cgcatccgc ttctctttt aattaa
      mRNA sequence
      • >TC031600 RA
        taggtttat atttactac tatatacta cctatcaag 
        tatatatct atctgctgt cataataat aatacgaga 
        atacgagaa aaaaatggc agacgtaga aaatcaatc 
        cgcatcggc taatgctgc tgcttcgac gacttcacc 
        tcaagttca tcatcatgc taaaaagga gaagaaagc 
        aaaaaatcc tagcaattc tagtcgtga tctggcagc 
        gagaagatg tctcatcgt ctcgctccc cccaaagcg 
        acttcgacc aagccgaag cccacgctg aagccccct 
        gcgaccaag cagaaaaaa tccggtgag tctgttgcc 
        gatttactt aaacatcaa acaccaaaa cctcctccc 
        caaaccttc gctaccgcc actacctcc tccaacatg 
        caaaatatg caaatcctt tcccgctcc cgctctccc 
        atcaacaaa acccttgac aaacccgta tccccaatc 
        tcctctcgc gaatccagc atgtctgtt tccgagtca 
        gaatacaaa tataccgtc cgaaacctg ccccaacac 
        attgctacc caaaaatca ttctattct tttttaatc 
        acaacaatt aacctcaga tcaatctta accctgaaa 
        gtcaatttt aaccgaacc gcgctccta attacttct 
        gcccccatt accccagcc atcatcaat caattccgc 
        accgcatcc ggtgatgcc aacataact gtcgaccct 
        attaacaaa aaatcccta aatacccca ttcccaaaa 
        gtccctaca ttttccgtt gttatccgc agcgtcgaa 
        cacgacatt accgaagcc gatatcatg aaccaaaac 
        ccaatcctc aaaattaac cgcctccac agaattatc 
        tccaaaaaa accaatcgt cccacccca ttaatccgc 
        gtcatctcc gacgataaa cccacaata gatcacctt 
        ttaacccaa ggcatcacc atcttttcc cgcgtctac 
        gactgcgaa gtctcccat ccacctccc ccaactcca 
        ctgcaatgc tccaaatgc ttccaactg ggtcatggc 
        cccaatgat tgccccaac aaacccatt tgccccaaa 
        tgccccgaa tctcatcac ccatccaaa tgcaccgtc 
        attacagcc aaatgcccc ttctgccaa ggctcccac 
        cctgcatgg tcccgtcaa tgtccagtt atcaaagaa 
        acccccatt accgaaaat accccattc gtcccaaca 
        aaaatcatt gacccccct tccgagttt gccgaccga 
        gacatagcc gaagacccc gacagcccc cactctgac 
        cccaaccca acccaaact ctcctccga caagtcatc 
        gctttcttt gcaaaaatt cttttcgac atttttccc 
        cttcagaaa cccaaaatg caacaaatt attgaacaa 
        gcctcctta catgccttt gaaattatc accaaaatt 
        tcccactcc ggccaccgc atccgcttc tcttttaat 
        taaaaatgg ctactaact ttgcatgtg ttaacattt 
        gcggccttc gccgcaaat ttcccatac taaaacact 
        tcctcctca ccgacaaca tttctattc tagccctgt 
        cagaaacaa aattttctc atcaaattc acatccctc 
        actacacaa cttatcaaa acaactctc ctaaccccc 
        taacaaaag gcgtcgcgc ttttaatct ctaactctt 
        acgcttctt cactgcatg ctctccccg ctcaatttt 
        ctcaactag aagccgtcg ccgcaaatc tttttatta 
        ataatacta acatactaa taatttctt actacaatt 
        ctccaaacc agcctgttt ctctagacc tcttaacct 
        atgcatgcc aattcccta acgctctca ttcttggtg 
        attttaacg cccgacaca cagactttg gcgacacgc 
        acacaaacc ctaacggac gcatcctaa acaccgcac 
        ttcaaaacc tcccaattt gccgcctct ataacacaa 
        cccccacat ttattcatc aaggttgct ccatccccg 
        atcatattc ttgtttcag aacatctct acccccatg 
        tgaaccccc gagccagaa tcggaacaa ccgtgtcat 
        cagaccacc tgccgctca cgtcaaact tcacattca 
        acgtccctc agccccccg cacagccat tcaccatcc 
        acaagttta cccacgcag attgggaaa a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY