Status
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Gene information
General information
TC031089
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010705,TC010704
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451687.1: 49114..118165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031089
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04898

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_04899

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031089 RA
      MNDNFISEL ESAAAIIMA PPNLVSNEQ RHNAEAIFL 
      NFRKCKTPF VMCREILDK CQVHYVLFE AAETIKSAL 
      IREWSFLSD SDKYSLRQY LMHYISTKQ VPSFVRDRI 
      IQVIAIMVK RASVDDGGR ERGTILQEV ESIILNAEP 
      EKKILGFNI IANLMQEYA STVKSTDVG LPWEVHFKA 
      KKQFESTDL KRIFQFCVQ LLSEVVKND PPYPDNVLE 
      LTRHILKVT ESVLTWGYM SPLFPKRLI GIYESVYEA 
      DHAPSLKLS DNWSEIILS PQLLPLMFQ IFWKVREYD 
      GLSHHALTC LVQLASLNG GVLSSDAVR LEYLKSYMV 
      NFTNLVSSV TIKNKESLG ISNIVKKLI LFFIGDIQK 
      LTSQLQDSY LDELTRLTC SFCKGAALE DMSSDEEKY 
      YNDSFDNMM EAWTNILQE YGVNSNGSI QECAVQIFN 
      TYIQYHLGP PDGARQNHD VHEIEDNED IDRISFKDQ 
      LQTIGMFGR IVPGHALPI IYKLLEVNT EKLKISLQL 
      MESRAMNMN ESSNLDNLF EDIHWVILI AGHILCMDS 
      DGETPMIPS EMMQYSIEQ LRQNNSTLE SSLTVLASA 
      HQITNVPTD VDRCDHIIR IVSDVLKLC VVENSAAEA 
      KLGHFMSPE VSSTIMWFL KRWCLSYLL PVENYYQEI 
      SPVLIGAVG KDTEGAIFV VNFILDKIY SNICHFNSE 
      PILLRDTVD LLTALVCIK QKQSLCIVK SKSLWNLIT 
      LQEKLTPGR LPSTIHREL FKSFILAGM ALRDMQELN 
      GYFDQILRP LHTRFDRFI NQEDNFRAN YHKEEVQKE 
      IIDILESFI GIAKGSHMS TVQILFQFL APRLAELPK 
      ILTFYNNYQ VIVQLILEL FGQCAKNML CYLCQLDSK 
      KLYESTLAT VQAYAKCNG NKFSGETLA EENSFQDLA 
      LILDLLTFI LSKDCIDLC PNDEEVVTV TASDVSLFG 
      LNFIMPLMT LDLLKYPSL CSQYYRLLV LINDIYPEK 
      ICNLPPTLF QQLLSSIEL GLTQFSSDI AQACLDFIQ 
      GMTWYFFRN SLQQNPFCQ AMKPFLKML LDLTLSHQI 
      NSDLMSSAS TCIYALICC YEEEYTILV DGLIKSQAD 
      PLIADRLAA AFHNLTLNV AMIGGRQPK LKFRDNFDK 
      FIANVQGFL LVK
    CDS sequence
    • >TC031089 RA
      atgaatgat aatttcatc tctgagctg gaatcagcc 
      gccgctatc atcatggct cccccgaac ctcgtgagc 
      aacgaacag cgacacaac gccgaagca atttttcta 
      aattttcgc aaatgtaaa actcctttt gtgatgtgt 
      cgtgaaatt ttggacaag tgccaagtt cattatgtg 
      ctgtttgaa gcagctgaa accatcaaa agtgccctt 
      attagagag tggtcgttc ttgtccgat tcggataaa 
      tactcgttg aggcagtat ttgatgcat tatatttcg 
      acgaagcaa gtgccctct ttcgtgcga gacaggatc 
      atacaagtt attgcaatt atggtcaaa agggcgagt 
      gtagacgat ggggggcgt gaaaggggt accattttg 
      caagaagtt gaaagtatc atactcaac gcggagccg 
      gaaaaaaaa attttgggt ttcaacata atcgcaaat 
      ttgatgcaa gaatatgcg agcacggtg aaatcaact 
      gatgtaggg ctgccctgg gaggtgcat ttcaaggcc 
      aaaaaacaa tttgaatca actgacttg aaacgaatc 
      tttcaattt tgtgtccaa ttgttatct gaagttgtg 
      aaaaacgac cccccgtac cctgataat gtgctggaa 
      ttgacgagg catatctta aaagttacg gaaagtgtg 
      cttacatgg gggtatatg tcaccactc tttcctaaa 
      cgattaatt ggcatctac gagtcggtt tatgaggct 
      gatcatgcc ccatcgttg aaattgtcg gacaattgg 
      agcgaaatt attttgtcg cctcaattg ttacctctc 
      atgtttcaa attttttgg aaagtgaga gaatacgac 
      ggattgtct catcatgcg ttgacgtgt cttgtgcag 
      ttggcttcg ttgaatggt ggagttttg tcgtcagat 
      gctgtcaga ttggagtat ttgaagtct tacatggtt 
      aattttaca aatcttgtc tctagtgtc acaataaaa 
      aacaaagaa tcgctaggc atatccaac atcgtcaag 
      aaactaata ctttttttc attggcgac attcaaaaa 
      ttaacatca caacttcaa gattcgtat ttagatgaa 
      cttacacga ttgacgtgt tcattttgc aaaggtgca 
      gctttagaa gatatgtct tccgacgaa gaaaaatat 
      tacaacgat tcgttcgac aatatgatg gaagcatgg 
      acaaacatt ttgcaagag tatggtgtg aactctaac 
      ggaagtatt caagaatgt gcagtacaa atttttaat 
      acttatatt caatatcat ttgggacca ccagatggc 
      gcacgacaa aatcatgat gtccacgaa attgaagac 
      aacgaagat attgatagg atctcgttc aaggaccag 
      ttacagacg attgggatg tttgggagg attgtacct 
      ggacatgcg ttgcccatt atatacaaa ctattggaa 
      gtcaacacc gaaaaacta aaaatcagt ctacaactg 
      atggagtct cgtgcaatg aacatgaac gaatcgtcc 
      aacttggac aacctcttc gaggacatc cattgggtg 
      attctcatt gccggccac atcctgtgt atggacagc 
      gacggtgaa accccaatg attccctcc gaaatgatg 
      caatactcc atcgaacaa ctccgtcaa aataacagc 
      acactcgaa tcaagtctt accgttttg gcttcagcc 
      catcaaata accaatgtt ccgacagac gttgatcgc 
      tgtgatcac atcatacgt atcgtttct gatgttttg 
      aaactatgt gttgtggaa aattcggcg gctgaagcc 
      aaattggga cattttatg agtcccgaa gtgagttcg 
      acgattatg tggtttctg aaacgatgg tgtttgtcg 
      tatctttta ccggtggag aattattat caagagatc 
      agtcctgtt ttgatcggt gctgtcggg aaggataca 
      gaaggggcg atttttgtg gtgaatttt attctggac 
      aagatttat tcgaatatt tgtcatttc aattcggag 
      cctatttta ttgagggat acagtggat cttctgacg 
      gcgcttgtt tgcatcaaa caaaaacag tccttatgc 
      atcgtaaag agcaaaagc ctctggaac ctcataacc 
      ctccaagag aagctcact ccaggtcgc ctcccctcc 
      accatccat cgcgaacta tttaaaagt ttcatccta 
      gctggcatg gctctacgc gacatgcaa gaactcaac 
      ggctacttt gaccaaatc ctgcgcccg cttcacact 
      cgattcgac cgcttcatc aaccaagaa gacaacttc 
      cgcgccaac taccacaaa gaggaagtc caaaaggaa 
      atcatcgat attttggaa agcttcatc ggaattgcc 
      aaaggctcg cacatgtcc accgtccaa atcctcttc 
      caatttctg gctccgcgg ttggcagaa ctgccgaaa 
      atcctaacg ttttacaac aactatcaa gtgattgtg 
      caactgatt ttggagttg tttggacag tgtgctaag 
      aacatgttg tgttatttg tgtcaactt gatagtaag 
      aagttgtac gagagtacg ttggcaacg gtgcaagcg 
      tatgcgaaa tgtaacggg aataagttt agtggggag 
      actttagct gaagaaaat agtttccaa gatttggca 
      ttgattttg gacttgttg acatttatt ttatcgaag 
      gattgcatt gatttgtgt ccaaatgac gaggaagtg 
      gttacagtg acggcgtct gatgtttct ttgttcggt 
      ttgaatttt attatgcct ttgatgact ttagatttg 
      ctcaaatat ccaagtctt tgctcgcaa tattacaga 
      ttgttggtt ttgatcaat gatatttat ccggagaag 
      atttgtaat ctcccgccg actttgttt caacaactg 
      ttgagctcg atcgagttg ggtttgaca cagtttagc 
      tcggatata gcccaagca tgccttgac ttcattcaa 
      ggaatgact tggtacttt ttcaggaat tcgcttcag 
      cagaatccg ttttgtcaa gcaatgaaa ccgttcctc 
      aaaatgttg ctggatctc actttgtcg catcaaata 
      aattcggat ttgatgagt tcggccagt acttgtatc 
      tacgctctg atttgttgc tacgaggag gagtatacg 
      attttagtc gatggttta attaaatcg caagcggat 
      ccgttgatt gccgatagg ttggcagca gcttttcat 
      aatttgacg ttgaatgtt gccatgatt ggcggaaga 
      caacctaag ttgaagttt cgagataat ttcgataag 
      ttcatcgca aatgttcag ggattcttg ttggtgaaa 
      tag
    mRNA sequence
    • >TC031089 RA
      ctatattca ttcaaaatg aatgataat ttcatctct 
      gagctggaa tcagccgcc gctatcatc atggctccc 
      ccgaacctc gtgagcaac gaacagcga cacaacgcc 
      gaagcaatt tttctaaat tttcgcaaa tgtaaaact 
      ccttttgtg atgtgtcgt gaaattttg gacaagtgc 
      caagttcat tatgtgctg tttgaagca gctgaaacc 
      atcaaaagt gcccttatt agagagtgg tcgttcttg 
      tccgattcg gataaatac tcgttgagg cagtatttg 
      atgcattat atttcgacg aagcaagtg ccctctttc 
      gtgcgagac aggatcata caagttatt gcaattatg 
      gtcaaaagg gcgagtgta gacgatggg gggcgtgaa 
      aggggtacc attttgcaa gaagttgaa agtatcata 
      ctcaacgcg gagccggaa aaaaaaatt ttgggtttc 
      aacataatc gcaaatttg atgcaagaa tatgcgagc 
      acggtgaaa tcaactgat gtagggctg ccctgggag 
      gtgcatttc aaggccaaa aaacaattt gaatcaact 
      gacttgaaa cgaatcttt caattttgt gtccaattg 
      ttatctgaa gttgtgaaa aacgacccc ccgtaccct 
      gataatgtg ctggaattg acgaggcat atcttaaaa 
      gttacggaa agtgtgctt acatggggg tatatgtca 
      ccactcttt cctaaacga ttaattggc atctacgag 
      tcggtttat gaggctgat catgcccca tcgttgaaa 
      ttgtcggac aattggagc gaaattatt ttgtcgcct 
      caattgtta cctctcatg tttcaaatt ttttggaaa 
      gtgagagaa tacgacgga ttgtctcat catgcgttg 
      acgtgtctt gtgcagttg gcttcgttg aatggtgga 
      gttttgtcg tcagatgct gtcagattg gagtatttg 
      aagtcttac atggttaat tttacaaat cttgtctct 
      agtgtcaca ataaaaaac aaagaatcg ctaggcata 
      tccaacatc gtcaagaaa ctaatactt tttttcatt 
      ggcgacatt caaaaatta acatcacaa cttcaagat 
      tcgtattta gatgaactt acacgattg acgtgttca 
      ttttgcaaa ggtgcagct ttagaagat atgtcttcc 
      gacgaagaa aaatattac aacgattcg ttcgacaat 
      atgatggaa gcatggaca aacattttg caagagtat 
      ggtgtgaac tctaacgga agtattcaa gaatgtgca 
      gtacaaatt tttaatact tatattcaa tatcatttg 
      ggaccacca gatggcgca cgacaaaat catgatgtc 
      cacgaaatt gaagacaac gaagatatt gataggatc 
      tcgttcaag gaccagtta cagacgatt gggatgttt 
      gggaggatt gtacctgga catgcgttg cccattata 
      tacaaacta ttggaagtc aacaccgaa aaactaaaa 
      atcagtcta caactgatg gagtctcgt gcaatgaac 
      atgaacgaa tcgtccaac ttggacaac ctcttcgag 
      gacatccat tgggtgatt ctcattgcc ggccacatc 
      ctgtgtatg gacagcgac ggtgaaacc ccaatgatt 
      ccctccgaa atgatgcaa tactccatc gaacaactc 
      cgtcaaaat aacagcaca ctcgaatca agtcttacc 
      gttttggct tcagcccat caaataacc aatgttccg 
      acagacgtt gatcgctgt gatcacatc atacgtatc 
      gtttctgat gttttgaaa ctatgtgtt gtggaaaat 
      tcggcggct gaagccaaa ttgggacat tttatgagt 
      cccgaagtg agttcgacg attatgtgg tttctgaaa 
      cgatggtgt ttgtcgtat cttttaccg gtggagaat 
      tattatcaa gagatcagt cctgttttg atcggtgct 
      gtcgggaag gatacagaa ggggcgatt tttgtggtg 
      aattttatt ctggacaag atttattcg aatatttgt 
      catttcaat tcggagcct attttattg agggataca 
      gtggatctt ctgacggcg cttgtttgc atcaaacaa 
      aaacagtcc ttatgcatc gtaaagagc aaaagcctc 
      tggaacctc ataaccctc caagagaag ctcactcca 
      ggtcgcctc ccctccacc atccatcgc gaactattt 
      aaaagtttc atcctagct ggcatggct ctacgcgac 
      atgcaagaa ctcaacggc tactttgac caaatcctg 
      cgcccgctt cacactcga ttcgaccgc ttcatcaac 
      caagaagac aacttccgc gccaactac cacaaagag 
      gaagtccaa aaggaaatc atcgatatt ttggaaagc 
      ttcatcgga attgccaaa ggctcgcac atgtccacc 
      gtccaaatc ctcttccaa tttctggct ccgcggttg 
      gcagaactg ccgaaaatc ctaacgttt tacaacaac 
      tatcaagtg attgtgcaa ctgattttg gagttgttt 
      ggacagtgt gctaagaac atgttgtgt tatttgtgt 
      caacttgat agtaagaag ttgtacgag agtacgttg 
      gcaacggtg caagcgtat gcgaaatgt aacgggaat 
      aagtttagt ggggagact ttagctgaa gaaaatagt 
      ttccaagat ttggcattg attttggac ttgttgaca 
      tttatttta tcgaaggat tgcattgat ttgtgtcca 
      aatgacgag gaagtggtt acagtgacg gcgtctgat 
      gtttctttg ttcggtttg aattttatt atgcctttg 
      atgacttta gatttgctc aaatatcca agtctttgc 
      tcgcaatat tacagattg ttggttttg atcaatgat 
      atttatccg gagaagatt tgtaatctc ccgccgact 
      ttgtttcaa caactgttg agctcgatc gagttgggt 
      ttgacacag tttagctcg gatatagcc caagcatgc 
      cttgacttc attcaagga atgacttgg tactttttc 
      aggaattcg cttcagcag aatccgttt tgtcaagca 
      atgaaaccg ttcctcaaa atgttgctg gatctcact 
      ttgtcgcat caaataaat tcggatttg atgagttcg 
      gccagtact tgtatctac gctctgatt tgttgctac 
      gaggaggag tatacgatt ttagtcgat ggtttaatt 
      aaatcgcaa gcggatccg ttgattgcc gataggttg 
      gcagcagct tttcataat ttgacgttg aatgttgcc 
      atgattggc ggaagacaa cctaagttg aagtttcga 
      gataatttc gataagttc atcgcaaat gttcaggga 
      ttcttgttg gtgaaatag ctgtgagtt gaattggct 
      tcgggtcaa aagatcgac gatgatttt ggttttaga 
      aaaattttt tatttgcga tgattcatc catcttgta 
      aagaatgtt cgccgtttt ttgacgtct gattaactt 
      acaaaaact tgtaactat atttttata caatgtaac 
      catgtctgt tatgcactt tataaatgc aataataaa 
      ttacatgag ttttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY