Status
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Gene information
General information
TC010672 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8044929..8062199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010672
    iBeetle screen iB_04894

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi) ovary without detectable defects - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010672 RA
      MFRLIGITL LLGRLGLAQ HLLDESVFT VNGKKALHT 
      VSDKFLSLS IDPAVLLTG LNLSDTTLQ MAKRLSPAY 
      IRIAGPSTQ YVKYQEESS NEAENGNVA VTPTMWFGI 
      NEWLASANL TPVFGINDR ETTRDGWNP QDAMPLLDI 
      SDKFNVSCY WQLGYDCAN KSEAEYESD LKRLQHVLD 
      AFPDKKRWK IVGSDLRRC VTDTRRILD NLDNVLAAA 
      ILEPTTKSD TIYVQQLNE WHILPKVPL WTVSPKSTH 
      SVSFDSAIS WAKQLGEAA KIGYHVILR QPRLHEIYS 
      ETPPFWVSA IHKNLMGRT VLDVKPTIG HAGLSVYAH 
      CTRHQNDFI RSGAMTVLV VNNCTTNHT LLLKFGTVL 
      LKNTEVQSY ILTGEDATN VLLNGEKLS LDKYTGDKL 
      NVLPKLRRA RIMNYLSLF VPPKSVAFF VLPGAQIPV 
      CASEENDLK QLIEEISED QNIPFSAEV SVDVTPRLA 
      ERKTPTLHE LQEVMMKEL ESDERFYKQ IKTKKSGHE 
      SDARKFFIE QTKHRHFDD YKRPKHKDE ETKKIFELE 
      LTSAEIRKV LADRAKAKA AKKHIIFTS DELEEIMDK 
      ATEKILNSK NAAIRIRKK SKREAPRDK KDINMRLLK 
      LKTLGDSRR KLLRETLNK RKAKAEQNR EKRDINMDL 
      LKLKTEMLH PNPNKPRKL KKKVFENKE EKQATIREP 
      GQTSDEFVQ FEEEKEESP LPDGDVFAE LADPDEAFF 
      TTEKPPKFG LKTIFNKPK PKIDVQPIK FEPSDLVAE 
      DDFEVNSDF DDNNEYYDC IHEFFGKSV PKDKQAKNS 
      SELWEVGLI EEKTDNHLP TLHNRGFAV DEAEVEKHE 
      EFVENDYED LDSDESSPL RKKRSIDEL SADTKLFYI 
      LGQGYDPAK VKIDDNLKE QQFKFLKDH LANHKKLEL 
      VHQLNVRSL ENKNFLADR YKSLAEGLK VWKPADILT 
      LKSPNAKRS RRSLDHDAD SVENEIGSF MNEIIKNTE 
      MIQPKLDRE NGDYQLVFP NKDKIEVVE KNGDAELKL 
      VLHGGEPTC PTTEKVENT RTNIQKITK KVPANVWDK 
      LFRKLSKFF QGLSSKLRR LSSRCANFT KYIARDRAR 
      VVQQAQETK IAIEPVDRT WSFSLVKKF VFLLRSERQ 
      RSRRTREHQ RRNIRNLDD QSRCDVLED EKSEEYHRK 
      NVAERRHRR RNRREVLDR WWLRSKREL RSLLSDQSF 
      GFGGVNSVC NFSKAQTHR QDLQWKLVT CRNNRRRRG 
      ALFIRGNVA LIEFWSEEL LSRCRFPSK GVNLTPLGS 
      RYKDAANTV KGTRKMLGK DLLRIKFSS KRSARPYLY 
      RQWFRAKDV QRRPSYGFI LPVVNGRLK TPTTIRPAY 
      TGEIRATRI NCRRDNTIP GNLNKITMG EIANQAFVM 
      NAIHTPA
    CDS sequence
    • >TC010672 RA
      atgttccgc ttgatcgga atcacactc cttttgggg 
      cgcctcggc ctcgcccag cacctgctg gacgagtcc 
      gtcttcacc gtgaacggc aagaaggcc ctacacacc 
      gtcagcgat aaatttctg agcctctcg atcgatcct 
      gccgtcctc ctgaccggt ctgaacttg agcgatacg 
      accctccaa atggccaag cgcctcagc ccggcctac 
      atcaggatc gccgggccc tccacacag tacgtaaaa 
      taccaggag gagagctcg aacgaggcc gaaaacggc 
      aatgtggct gtcacgccg acgatgtgg ttcgggata 
      aatgagtgg ctggcgtcg gccaatctc accccagtt 
      ttcggaatt aatgatcgg gagaccacg agggatgga 
      tggaatccg caggatgcg atgccgttg ttggatatt 
      tcggataag tttaatgtg agctgctat tggcagctg 
      gggtatgac tgcgcgaat aaaagcgag gcggagtat 
      gaaagcgac ctgaagcgg ctgcagcat gtcctggac 
      gcttttccc gacaagaag cgctggaag atcgtcggg 
      agcgatttg aggcgatgc gtcactgac accagacga 
      attttggac aatttggac aatgtttta gctgcggcg 
      atcctcgaa ccaacgacc aaaagtgac acaatctac 
      gtgcaacaa ttgaacgag tggcatatt ttacccaaa 
      gtgccgctt tggacggtc tcccccaag tccactcat 
      tcggtttcc ttcgactcg gccatttca tgggccaaa 
      caactggga gaggcggct aaaatcggc taccacgtt 
      attctgcga cagccacgt ctccacgaa atttattcg 
      gaaacgccc ccgttttgg gtctccgca attcataaa 
      aatctgatg gggaggacc gttttggac gtgaaaccc 
      accattggg cacgcgggg ttgtcggtt tacgcccat 
      tgtaccaga caccaaaac gatttcatc cgcagtggc 
      gctatgacc gttcttgtt gtgaacaac tgtaccaca 
      aatcacaca ctcctccta aaatttggc acagtttta 
      ctgaaaaac accgaggtc cagtcttat atcctgacg 
      ggcgaggat gcgacaaac gttttacta aatggcgaa 
      aaattaagt ctggacaaa tacacaggc gataaactt 
      aacgtttta ccaaaattg aggcgtgcc aggatcatg 
      aactactta tccttgttt gtgccgcca aaatcagta 
      gcgttcttt gttttaccg ggtgcccaa attccggtg 
      tgtgcgagt gaagaaaac gatttgaaa caactgatt 
      gaggagata agtgaggac cagaatata ccgttctcg 
      gcggaagtt tcagttgat gtaactcca agacttgcc 
      gaacgtaaa acaccaact ttgcacgaa ttgcaggaa 
      gttatgatg aaagaactg gagtcggac gaacgcttc 
      tacaagcaa attaaaacg aagaagagt ggacacgaa 
      agtgatgct aggaaattt tttatcgaa caaacgaaa 
      cacagacat ttcgacgac tacaaaagg cccaaacat 
      aaggacgag gaaacgaag aaaattttt gaactggag 
      ttgacaagc gctgaaatt cgcaaagtt ctagccgac 
      cgtgccaaa gctaaagcc gcaaagaag cacatcatt 
      ttcacgagt gacgagttg gaggagatt atggacaaa 
      gcgaccgag aaaattttg aatagtaaa aatgccgcg 
      attcggatt cggaaaaaa tcgaagaga gaggcgcca 
      cgtgataaa aaagacatt aacatgaga ttgttgaaa 
      ttgaagact ttgggtgat agtagaagg aaattgctg 
      cgcgaaacg ctcaacaaa cgaaaggct aaagcggag 
      caaaaccgt gagaaacgg gacattaac atggatttg 
      ctcaagctg aagaccgaa atgctgcac ccgaacccg 
      aacaagccc aggaaattg aagaaaaag gtgtttgag 
      aacaaggaa gagaaacag gcgacgatt cgcgagccg 
      ggacagacc tcggacgag ttcgtccag tttgaggag 
      gaaaaggag gagagtccg ttgcccgac ggtgacgtt 
      ttcgccgaa ctagcggac cccgatgag gcttttttt 
      accacagag aaaccaccg aaattcgga ctgaaaacc 
      atctttaat aaacccaag ccgaagatt gacgtgcag 
      ccgattaag ttcgaaccg tctgatttg gtcgctgag 
      gacgacttt gaggtgaat tcggacttc gatgacaac 
      aacgaatat tacgactgc atccacgag tttttcggg 
      aaaagcgtt ccgaaggat aaacaagcg aaaaactcg 
      tccgaattg tgggaggtc ggcttgatt gaagagaaa 
      acggacaac cacttgccc actttgcat aatcgcggg 
      tttgctgtt gatgaagcc gaagtcgaa aaacacgag 
      gaatttgtc gaaaatgat tacgaggac ctggacagt 
      gacgaaagt agtccgttg cgcaaaaaa cgaagcatt 
      gatgaatta agtgctgac acgaagctt ttttacatt 
      ttaggtcag ggttacgat cctgcgaag gtgaagatt 
      gacgataat ttgaaggag caacagttt aagtttttg 
      aaggaccat ttggctaat cacaagaag cttgaactg 
      gtgcatcag ctcaatgtt cgatcgctt gagaacaag 
      aatttccta gccgatcgg tacaagtcc ttggctgag 
      ggcttgaaa gtgtggaaa cctgcggat attttgacg 
      ctgaaaagc ccgaatgcg aagaggtcg aggcggagt 
      cttgatcac gatgcggat tcggtcgag aacgaaatt 
      gggagtttc atgaacgag attattaag aatacggag 
      atgattcag ccgaaactc gatcgggag aacggcgac 
      tatcagttg gtttttcca aacaaggat aagatcgaa 
      gtcgttgag aaaaacggc gatgcggaa ttgaaattg 
      gtgcttcat ggaggcgag ccgacttgt ccaacgacc 
      gagaaagtg gagaacact cgtacaaac attcagaag 
      atcacgaaa aaagtgccg gccaacgtg tgggacaaa 
      cttttccga aaattgtcg aaatttttc cagggtttg 
      agctcgaaa ttgcggaga ttgtcgtct agatgtgct 
      aatttcaca aaatatatc gcaagagac agagcccga 
      gtagtacag caagcgcaa gaaacaaag atcgcaatc 
      gagcccgtc gacaggacc tggagcttt tctttggta 
      aaaaagttt gtttttctc ctgcgctcg gagcgccag 
      cgctctcga cggactcgg gaacatcaa aggcgaaat 
      ataaggaat ttggatgac caatcacgg tgtgatgtg 
      ctggaggac gagaagagt gaagagtat catcggaaa 
      aacgtcgct gagaggcgc catcggcgg aggaaccgg 
      cgcgaggtc ctggaccgc tggtggtta cgctcaaaa 
      agagaatta cgttcgtta ctctcggat caaagtttc 
      ggtttcgga ggcgtaaac tcggtctgc aacttttcg 
      aaggctcag acacaccgc caagatttg caatggaaa 
      ttggtcacg tgtcgaaac aatcgtcgg cgccgaggc 
      gcgcttttt attcgagga aacgtcgcg ttaatcgag 
      ttttggagc gaagagctg ctctccagg tgtagattt 
      ccctcaaag ggagtcaac ctgactccg ttagggagt 
      cgctataaa gatgctgca aatacggtt aaaggtaca 
      agaaaaatg ttaggaaaa gatttgttg agaattaaa 
      ttttcttca aaaaggtca gcgagacca tatctctat 
      cgccaatgg tttagggct aaggacgtt caaagacgc 
      ccaagctac ggattcatt ttaccggtg gtcaacggc 
      cgtttaaaa acgccgacg accatccgg ccggcgtac 
      accggagaa atccgggcc acacgcatt aattgccgt 
      cgagataat acgattccg ggcaatttg aataaaatt 
      accatggga gaaattgca aatcaagcc ttcgtgatg 
      aacgcaatc cacacgcct gcataa
    mRNA sequence
    • >TC010672 RA
      atgttccgc ttgatcgga atcacactc cttttgggg 
      cgcctcggc ctcgcccag cacctgctg gacgagtcc 
      gtcttcacc gtgaacggc aagaaggcc ctacacacc 
      gtcagcgat aaatttctg agcctctcg atcgatcct 
      gccgtcctc ctgaccggt ctgaacttg agcgatacg 
      accctccaa atggccaag cgcctcagc ccggcctac 
      atcaggatc gccgggccc tccacacag tacgtaaaa 
      taccaggag gagagctcg aacgaggcc gaaaacggc 
      aatgtggct gtcacgccg acgatgtgg ttcgggata 
      aatgagtgg ctggcgtcg gccaatctc accccagtt 
      ttcggaatt aatgatcgg gagaccacg agggatgga 
      tggaatccg caggatgcg atgccgttg ttggatatt 
      tcggataag tttaatgtg agctgctat tggcagctg 
      gggtatgac tgcgcgaat aaaagcgag gcggagtat 
      gaaagcgac ctgaagcgg ctgcagcat gtcctggac 
      gcttttccc gacaagaag cgctggaag atcgtcggg 
      agcgatttg aggcgatgc gtcactgac accagacga 
      attttggac aatttggac aatgtttta gctgcggcg 
      atcctcgaa ccaacgacc aaaagtgac acaatctac 
      gtgcaacaa ttgaacgag tggcatatt ttacccaaa 
      gtgccgctt tggacggtc tcccccaag tccactcat 
      tcggtttcc ttcgactcg gccatttca tgggccaaa 
      caactggga gaggcggct aaaatcggc taccacgtt 
      attctgcga cagccacgt ctccacgaa atttattcg 
      gaaacgccc ccgttttgg gtctccgca attcataaa 
      aatctgatg gggaggacc gttttggac gtgaaaccc 
      accattggg cacgcgggg ttgtcggtt tacgcccat 
      tgtaccaga caccaaaac gatttcatc cgcagtggc 
      gctatgacc gttcttgtt gtgaacaac tgtaccaca 
      aatcacaca ctcctccta aaatttggc acagtttta 
      ctgaaaaac accgaggtc cagtcttat atcctgacg 
      ggcgaggat gcgacaaac gttttacta aatggcgaa 
      aaattaagt ctggacaaa tacacaggc gataaactt 
      aacgtttta ccaaaattg aggcgtgcc aggatcatg 
      aactactta tccttgttt gtgccgcca aaatcagta 
      gcgttcttt gttttaccg ggtgcccaa attccggtg 
      tgtgcgagt gaagaaaac gatttgaaa caactgatt 
      gaggagata agtgaggac cagaatata ccgttctcg 
      gcggaagtt tcagttgat gtaactcca agacttgcc 
      gaacgtaaa acaccaact ttgcacgaa ttgcaggaa 
      gttatgatg aaagaactg gagtcggac gaacgcttc 
      tacaagcaa attaaaacg aagaagagt ggacacgaa 
      agtgatgct aggaaattt tttatcgaa caaacgaaa 
      cacagacat ttcgacgac tacaaaagg cccaaacat 
      aaggacgag gaaacgaag aaaattttt gaactggag 
      ttgacaagc gctgaaatt cgcaaagtt ctagccgac 
      cgtgccaaa gctaaagcc gcaaagaag cacatcatt 
      ttcacgagt gacgagttg gaggagatt atggacaaa 
      gcgaccgag aaaattttg aatagtaaa aatgccgcg 
      attcggatt cggaaaaaa tcgaagaga gaggcgcca 
      cgtgataaa aaagacatt aacatgaga ttgttgaaa 
      ttgaagact ttgggtgat agtagaagg aaattgctg 
      cgcgaaacg ctcaacaaa cgaaaggct aaagcggag 
      caaaaccgt gagaaacgg gacattaac atggatttg 
      ctcaagctg aagaccgaa atgctgcac ccgaacccg 
      aacaagccc aggaaattg aagaaaaag gtgtttgag 
      aacaaggaa gagaaacag gcgacgatt cgcgagccg 
      ggacagacc tcggacgag ttcgtccag tttgaggag 
      gaaaaggag gagagtccg ttgcccgac ggtgacgtt 
      ttcgccgaa ctagcggac cccgatgag gcttttttt 
      accacagag aaaccaccg aaattcgga ctgaaaacc 
      atctttaat aaacccaag ccgaagatt gacgtgcag 
      ccgattaag ttcgaaccg tctgatttg gtcgctgag 
      gacgacttt gaggtgaat tcggacttc gatgacaac 
      aacgaatat tacgactgc atccacgag tttttcggg 
      aaaagcgtt ccgaaggat aaacaagcg aaaaactcg 
      tccgaattg tgggaggtc ggcttgatt gaagagaaa 
      acggacaac cacttgccc actttgcat aatcgcggg 
      tttgctgtt gatgaagcc gaagtcgaa aaacacgag 
      gaatttgtc gaaaatgat tacgaggac ctggacagt 
      gacgaaagt agtccgttg cgcaaaaaa cgaagcatt 
      gatgaatta agtgctgac acgaagctt ttttacatt 
      ttaggtcag ggttacgat cctgcgaag gtgaagatt 
      gacgataat ttgaaggag caacagttt aagtttttg 
      aaggaccat ttggctaat cacaagaag cttgaactg 
      gtgcatcag ctcaatgtt cgatcgctt gagaacaag 
      aatttccta gccgatcgg tacaagtcc ttggctgag 
      ggcttgaaa gtgtggaaa cctgcggat attttgacg 
      ctgaaaagc ccgaatgcg aagaggtcg aggcggagt 
      cttgatcac gatgcggat tcggtcgag aacgaaatt 
      gggagtttc atgaacgag attattaag aatacggag 
      atgattcag ccgaaactc gatcgggag aacggcgac 
      tatcagttg gtttttcca aacaaggat aagatcgaa 
      gtcgttgag aaaaacggc gatgcggaa ttgaaattg 
      gtgcttcat ggaggcgag ccgacttgt ccaacgacc 
      gagaaagtg gagaacact cgtacaaac attcagaag 
      atcacgaaa aaagtgccg gccaacgtg tgggacaaa 
      cttttccga aaattgtcg aaatttttc cagggtttg 
      agctcgaaa ttgcggaga ttgtcgtct agatgtgct 
      aatttcaca aaatatatc gcaagagac agagcccga 
      gtagtacag caagcgcaa gaaacaaag atcgcaatc 
      gagcccgtc gacaggacc tggagcttt tctttggta 
      aaaaagttt gtttttctc ctgcgctcg gagcgccag 
      cgctctcga cggactcgg gaacatcaa aggcgaaat 
      ataaggaat ttggatgac caatcacgg tgtgatgtg 
      ctggaggac gagaagagt gaagagtat catcggaaa 
      aacgtcgct gagaggcgc catcggcgg aggaaccgg 
      cgcgaggtc ctggaccgc tggtggtta cgctcaaaa 
      agagaatta cgttcgtta ctctcggat caaagtttc 
      ggtttcgga ggcgtaaac tcggtctgc aacttttcg 
      aaggctcag acacaccgc caagatttg caatggaaa 
      ttggtcacg tgtcgaaac aatcgtcgg cgccgaggc 
      gcgcttttt attcgagga aacgtcgcg ttaatcgag 
      ttttggagc gaagagctg ctctccagg tgtagattt 
      ccctcaaag ggagtcaac ctgactccg ttagggagt 
      cgctataaa gatgctgca aatacggtt aaaggtaca 
      agaaaaatg ttaggaaaa gatttgttg agaattaaa 
      ttttcttca aaaaggtca gcgagacca tatctctat 
      cgccaatgg tttagggct aaggacgtt caaagacgc 
      ccaagctac ggattcatt ttaccggtg gtcaacggc 
      cgtttaaaa acgccgacg accatccgg ccggcgtac 
      accggagaa atccgggcc acacgcatt aattgccgt 
      cgagataat acgattccg ggcaatttg aataaaatt 
      accatggga gaaattgca aatcaagcc ttcgtgatg 
      aacgcaatc cacacgcct gcataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY