General information | |||
TC010668 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 8181863..8183147 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC010668 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04892 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
- >TC010668 RA
MAEQVFDDW RELSQDFKK LEAVNKTYQ EKLDELNKY QKECQKQIQ HQKYRIGII NNTIKRSKH DRYEELKEE IFKREAQLQ DIQQTLPKE NGRYLKVIL GNVNVSILN KEDKLKYKS EYEKFKLIV SALAFVFSV VNLLFISRR LERLYLFIL VWYYSTLTI RESILRVNG SKIKGWWMV HHYISAITA AILLIWPES DTWNQFRTQ FMFFNAYIS VVQYLQFRY QQGVLYRLK ALGVRDNMD ITIEGFHHW MWRGLSFLF PFLFVVYFY QLYLAYSLY NLSYVSGAT WHVSALSKL FLTLSVGNI TTMMMIIPD KFKNNVRLK YRVLSQKLF SDSESKNKK NKKQ
- >TC010668 RA
atggccgag caagtgttt gacgattgg cgcgaatta agtcaagac tttaaaaaa ctcgaagcc gttaataaa acatatcaa gagaaactc gacgagctc aacaagtac caaaaagag tgtcagaaa cagatccag catcaaaaa taccgcatc ggtatcatc aacaacacc atcaaacgc tccaaacac gaccgatac gaagaactc aaggaggag atttttaaa cgtgaggcg caacttcaa gacatccag caaacactc ccgaaggaa aatggaagg tacttgaaa gtgattttg ggcaacgtc aatgtctcg attctgaat aaagaggac aagctcaag tacaagagc gagtatgag aagttcaag ctgattgtg agtgcgttg gcgttcgtg ttttcggtc gtcaatttg ctttttata agccggaga ttggagcgt ctgtatttg tttatactg gtgtggtac tattccact ctcactatc agagagagt attctgagg gtcaacggt tccaaaatc aagggctgg tggatggtg catcactac atctcggcc ataacggcg gcaattctc ctaatttgg cccgaaagc gacacttgg aaccagttc aggacgcag tttatgttt ttcaatgcg tacatcagt gtggtgcag tacttgcag tttcggtac caacaggga gtgctctac cgacttaaa gccctgggc gtgagggac aatatggac attacgatt gaagggttc caccactgg atgtggcgg ggacttagc ttcctgttc ccgtttttg ttcgttgtg tacttctac caactctat ttagcttac tcgctgtat aatttaagt tacgtaagc ggtgccacc tggcacgtt agtgcctta tcaaaattg tttttaact ttatcagtt ggaaacatc acgaccatg atgatgatt atccccgat aagtttaag aataatgtg aggctaaag tacagggta cttagtcag aaattgttc agcgatagc gaaagcaaa aacaagaaa aataaaaaa caataa
- >TC010668 RA
gttttttta ataaaccga tcataaatc caatgtgag tgctccaag tgtgacgat ggccgagca agtgtttga cgattggcg cgaattaag tcaagactt taaaaaact cgaagccgt taataaaac atatcaaga gaaactcga cgagctcaa caagtacca aaaagagtg tcagaaaca gatccagca tcaaaaata ccgcatcgg tatcatcaa caacaccat caaacgctc caaacacga ccgatacga agaactcaa ggaggagat ttttaaacg tgaggcgca acttcaaga catccagca aacactccc gaaggaaaa tggaaggta cttgaaagt gattttggg caacgtcaa tgtctcgat tctgaataa agaggacaa gctcaagta caagagcga gtatgagaa gttcaagct gattgtgag tgcgttggc gttcgtgtt ttcggtcgt caatttgct ttttataag ccggagatt ggagcgtct gtatttgtt tatactggt gtggtacta ttccactct cactatcag agagagtat tctgagggt caacggttc caaaatcaa gggctggtg gatggtgca tcactacat ctcggccat aacggcggc aattctcct aatttggcc cgaaagcga cacttggaa ccagttcag gacgcagtt tatgttttt caatgcgta catcagtgt ggtgcagta cttgcagtt tcggtacca acagggagt gctctaccg acttaaagc cctgggcgt gagggacaa tatggacat tacgattga agggttcca ccactggat gtggcgggg acttagctt cctgttccc gtttttgtt cgttgtgta cttctacca actctattt agcttactc gctgtataa tttaagtta cgtaagcgg tgccacctg gcacgttag tgccttatc aaaattgtt tttaacttt atcagttgg aaacatcac gaccatgat gatgattat ccccgataa gtttaagaa taatgtgag gctaaagta cagggtact tagtcagaa attgttcag cgatagcga aagcaaaaa caagaaaaa taaaaaaca ataaagata ttatttata gcgcacttg tttatttct tatcttcct caggtacat catcacatt catcgctta cactattat tattttgac cgtcgtttt cattttgtt catcactta ttgcttcca ccatttcac aatcgatcg tttcgcatt ccgagacgt tttcttcac ttggctccg catttactt cttcttc
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
