Status
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Gene information
General information
TC010654 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8260917..8293482
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010654
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04890

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    head segmentation/division not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax dorsoventral-axis thinner - Penetrance (percentage of animals/eggs on slide): more than 80%

    hindgut everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen dorsoventral-axis thinner - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010654 RA
      MPRRVLLLL LGAFSIVHS ARNVTISKC CPFTATLNN 
      ENKCIPVLT PKWTLKVFS PRNRSYLPD NQVPPNWHL 
      KPASKPPCA MPALLTPNL GNYIPFQNG SLYVLEYDE 
      VVHPDNFCI DYAAVLVCL KAQQPESMT AVRVKKCCG 
      ENAIFSETN KTCIHFKDA GYKIDVGSD KKLTAGFPT 
      CDHQEVVIT EMLREAEMH KNGSLWLPK MKVLLPAAN 
      YCLENILED AGHSPKIMI CQEHVTVEK AESHDIRFI 
      IYPISLALS AVFLAATLA AGAILPASH HVLHWRCQT 
      NHVACLLVG NVLLCITQL SGRMDATLC FSIAVSMHF 
      LFLAAFFWL NTMCFNIWW TFRDLRPQS VEKSQEWYR 
      LRLYELYAW GVPVVIAGT AAILDQVAD SSNFLRPKF 
      GDNTCWFHG NLEKLTFFY GPIGTLLLI NLALFALTA 
      RELTCGLWK RELVKSTSE RAALGRVCI KLVVVMGVS 
      WIAEIISWM VGGPQELWY LTDLINCFQ GVFIFIVVG 
      CQPQVLSAV KRLWCLRKH RANGTAGTT NHHSSSSQG 
      MPSMGDTVT NHSVTNNTT KSVPLETSC
    CDS sequence
    • >TC010654 RA
      atgccgcgc cgcgtcctc ctgctcctc ctcggcgcc 
      ttctccata gtgcactcc gcgcggaac gtcaccatc 
      tccaagtgc tgccccttc accgccacc ctcaacaac 
      gagaacaag tgcatcccc gtgctgacc ccgaagtgg 
      accctcaaa gtgttcagc ccgcgcaac cgcagctac 
      ctccccgac aaccaggtg ccccccaac tggcacctg 
      aagccggcg tccaagccc ccgtgcgcc atgccggcg 
      ctgctcacc cccaacctg gggaactac atccccttc 
      cagaacggc tcgctctac gtgctggag tacgacgag 
      gtggtgcac ccggacaac ttctgcata gactatgcc 
      gcggttctg gtgtgtctt aaggcccag cagccggag 
      agtatgacg gctgttagg gtgaagaag tgctgcggg 
      gaaaatgcc atcttctcc gagaccaac aaaacgtgc 
      attcatttc aaagacgcg ggatacaag atcgatgtc 
      gggtccgat aagaaactg accgcgggg ttccccacg 
      tgcgaccac caggaggtc gtcatcacg gagatgcta 
      agggaagcg gaaatgcac aaaaatggg agcttgtgg 
      ctgccgaaa atgaaggtg ctcctacct gccgccaat 
      tactgtctg gagaacatc ctcgaggat gccggtcat 
      tccccgaag ataatgatt tgccaggag cacgtgacg 
      gtggagaag gccgagagc cacgacatc cgcttcatc 
      atctacccc ataagtctg gcccttagt gcggttttt 
      ttggcggcg acgctggcg gcgggggcc atcctcccg 
      gccagccac cacgtgctg cactggcgg tgccagacc 
      aaccacgtg gcctgcctc ctcgtcggc aacgttctc 
      ctctgcata acgcagctc tcgggccgg atggacgcg 
      actctctgc ttctcaatc gctgtgtcg atgcatttc 
      ctgtttttg gcggccttc ttctggctc aacacaatg 
      tgtttcaac atctggtgg acttttcgc gatctgcgg 
      ccgcaaagc gtcgaaaag agccaagag tggtaccgt 
      ttacgtctt tacgaactc tacgcgtgg ggagtcccc 
      gtggtcatc gccggaacg gccgccatc ttggaccaa 
      gtggccgac agctccaac tttctccgg cccaagttc 
      ggggacaac acttgctgg ttccacggc aacttggag 
      aagctgacg ttcttctac ggccccatc ggcactctc 
      ctcctcatc aacctggcc ctgttcgcc ctcacggcc 
      cgcgagctg acttgcggg ttgtggaag agagagctg 
      gtcaagagc acgtccgag agggccgcg ctgggccgc 
      gtctgcatc aagctggtg gtggtgatg ggcgtcagc 
      tggatagcg gagatcatc tcctggatg gtgggtggc 
      ccacaagag ctgtggtac ttgaccgac ctgatcaac 
      tgcttccaa ggggtcttt atcttcatt gtggtggga 
      tgccagcct caggttttg tcggccgtg aagcggctg 
      tggtgcttg aggaagcac agggcgaat ggtaccgcg 
      ggtaccacc aaccatcac tcgtccagc tcgcaaggg 
      atgccgtcg atgggtgat actgtgacg aatcacagt 
      gttaccaac aatacgacc aagtcggtg ccgctggag 
      acgagttgc tga
    mRNA sequence
    • >TC010654 RA
      acacgcctt gatcgcact ggtgctcac acaaaggaa 
      aataaaatc agtcgacga aagcgcgtc ttcgtgagt 
      acactccca tgtgttaaa atgccgcgc cgcgtcctc 
      ctgctcctc ctcggcgcc ttctccata gtgcactcc 
      gcgcggaac gtcaccatc tccaagtgc tgccccttc 
      accgccacc ctcaacaac gagaacaag tgcatcccc 
      gtgctgacc ccgaagtgg accctcaaa gtgttcagc 
      ccgcgcaac cgcagctac ctccccgac aaccaggtg 
      ccccccaac tggcacctg aagccggcg tccaagccc 
      ccgtgcgcc atgccggcg ctgctcacc cccaacctg 
      gggaactac atccccttc cagaacggc tcgctctac 
      gtgctggag tacgacgag gtggtgcac ccggacaac 
      ttctgcata gactatgcc gcggttctg gtgtgtctt 
      aaggcccag cagccggag agtatgacg gctgttagg 
      gtgaagaag tgctgcggg gaaaatgcc atcttctcc 
      gagaccaac aaaacgtgc attcatttc aaagacgcg 
      ggatacaag atcgatgtc gggtccgat aagaaactg 
      accgcgggg ttccccacg tgcgaccac caggaggtc 
      gtcatcacg gagatgcta agggaagcg gaaatgcac 
      aaaaatggg agcttgtgg ctgccgaaa atgaaggtg 
      ctcctacct gccgccaat tactgtctg gagaacatc 
      ctcgaggat gccggtcat tccccgaag ataatgatt 
      tgccaggag cacgtgacg gtggagaag gccgagagc 
      cacgacatc cgcttcatc atctacccc ataagtctg 
      gcccttagt gcggttttt ttggcggcg acgctggcg 
      gcgggggcc atcctcccg gccagccac cacgtgctg 
      cactggcgg tgccagacc aaccacgtg gcctgcctc 
      ctcgtcggc aacgttctc ctctgcata acgcagctc 
      tcgggccgg atggacgcg actctctgc ttctcaatc 
      gctgtgtcg atgcatttc ctgtttttg gcggccttc 
      ttctggctc aacacaatg tgtttcaac atctggtgg 
      acttttcgc gatctgcgg ccgcaaagc gtcgaaaag 
      agccaagag tggtaccgt ttacgtctt tacgaactc 
      tacgcgtgg ggagtcccc gtggtcatc gccggaacg 
      gccgccatc ttggaccaa gtggccgac agctccaac 
      tttctccgg cccaagttc ggggacaac acttgctgg 
      ttccacggc aacttggag aagctgacg ttcttctac 
      ggccccatc ggcactctc ctcctcatc aacctggcc 
      ctgttcgcc ctcacggcc cgcgagctg acttgcggg 
      ttgtggaag agagagctg gtcaagagc acgtccgag 
      agggccgcg ctgggccgc gtctgcatc aagctggtg 
      gtggtgatg ggcgtcagc tggatagcg gagatcatc 
      tcctggatg gtgggtggc ccacaagag ctgtggtac 
      ttgaccgac ctgatcaac tgcttccaa ggggtcttt 
      atcttcatt gtggtggga tgccagcct caggttttg 
      tcggccgtg aagcggctg tggtgcttg aggaagcac 
      agggcgaat ggtaccgcg ggtaccacc aaccatcac 
      tcgtccagc tcgcaaggg atgccgtcg atgggtgat 
      actgtgacg aatcacagt gttaccaac aatacgacc 
      aagtcggtg ccgctggag acgagttgc tgacgcgtg 
      atacgagca tttttggac tatatagat gcgtttatt 
      tttttactc taacaaatt agactagtc agctattga 
      caatttgga cgtatgtgt gcagcattc cgttgtgtt 
      ctttttttt ttgactgat tattgtgta tttaggttt 
      agttccttt atttatgtt gtttgttac ctatgttaa 
      gtcgaagta catagtttt cttatttat cttttatga 
      tcttgtgtt ataaacgac gcccagtat tatatcact 
      tttggtagt tttgtttta gcctgtttt tgtgtatgt 
      tgtatgttt ttgtaaata aagagtaca aagtaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY