Status
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Gene information
General information
TC010653 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8245847..8253623
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010653
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04889

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    2 empty eggs

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010653 RA
      MLLLFVLLL ASSGSCQDS EGPYRGKHI GKLNSYHHE 
      VGGDVYAVD DYTLLLTSF SYDGNGADT FFWAGASNR 
      PGPQGFIVP DEYGKTNVL ARYLQKDFT LTLPDNKKI 
      TDIKWFAIY DLLSQNTFG DIYIPEEFE PPTVQKIPQ 
      LASKSHSVS SGAVEIIDA KRIRLNDFR YNGQAKRAH 
      FWVGVGAQP VSKGHKVPD ENGYLDPLR AYKGETITL 
      ELPGDLTIF NIAWFSVFD LETNENLGS IVIPDGLNV 
      PPSLVKVIP HEHHLPNCL QLHKDFQVS WDIFGPQIT 
      IQMAGQVDE NSYMAFGMS GSKEQSQML GSDVTVAYL 
      DGYRGYATD YNITALTPC VKVLGQYKG VCKDELVGG 
      QDSNQMHTA TRENGINII TFRRALISP DPGDQEYPT 
      EGSIYIIWA MGRLDHNKE PTFHDIYPK TNISIELQP 
      KEPSSNCFS FTRSDTQLR EPWVKGQIY DKTIRTFKA 
      YLGPSGGRR GYQGTTGQT STTLAWYVN GLLAPELWL 
      RRGLTYAFR VYGGNNPHS AEFYHPLII TDEPHGGFD 
      RLSDEAQAK IRVLAGVEY SRRGRPRPT AAGPLCLAK 
      HQDSNDRRL DGDFPTFKK FNRSLVYSC EEGEPGVLE 
      FTPNSSWPD IVYYNSFTQ SNMGWKIHI IDSFSSRTN 
      GISKMCGNI FVLVSILFV KQFV
    CDS sequence
    • >TC010653 RA
      atgttacta ctcttcgtt ttgctcttg gcaagttca 
      ggttcatgt caagacagt gaaggcccc taccgtggt 
      aaacacatc gggaagcta aactcgtac caccatgag 
      gtcgggggc gacgtgtac gcggtggac gactacacc 
      ctgctgcta acatcgttt agttatgac gggaacggg 
      gctgacacc ttcttctgg gcgggggcg tccaacagg 
      ccggggccc caggggttc atcgtgccg gacgaatac 
      ggaaagact aatgttttg gcgcgttat ttgcaaaaa 
      gacttcacg ctgacgctc cccgataat aaaaaaatc 
      accgatatt aagtggttt gccatttat gatcttttg 
      agtcaaaac actttcggg gacatttat attccggaa 
      gagttcgaa cctccaact gtacaaaaa ataccacag 
      ttggcctcc aaatcgcat tccgtgtct tcaggcgct 
      gttgaaatc attgacgct aagagaatc cgactgaac 
      gatttccgg tacaatgga caagccaaa agggcgcat 
      ttctgggtc ggagttggc gcccaacct gtgtccaaa 
      gggcacaaa gttcccgac gaaaacgga tatttggac 
      cctctgcgg gcctacaag ggcgagacc atcactctt 
      gagcttccc ggggacttg acaatattc aacatcgcg 
      tggtttagt gttttcgat cttgaaacc aacgaaaat 
      ttgggttct attgttata ccggacgga cttaacgtc 
      cctccgtct ctagtcaaa gttattccg cacgaacac 
      cacctacct aactgtttg caactccat aaagatttc 
      caagtgtcg tgggacatt tttgggcca caaattacc 
      atccaaatg gccggtcaa gtcgatgaa aattcctac 
      atggccttt ggtatgtct gggtcaaag gaacagagt 
      caaatgttg ggttctgat gtcactgtg gcctatttg 
      gacgggtat agaggctac gctaccgac tataacatt 
      acagctttg actccttgt gtcaaagtt ttgggacaa 
      tataaaggt gtgtgcaaa gacgagctt gtagggggg 
      caggacagc aatcaaatg cacacagct acgcgggaa 
      aacggcatc aatattatc acctttcgg cgcgctctt 
      atctcaccc gaccctggc gaccaggaa taccccacc 
      gagggctca atttacata atctgggca atgggccgc 
      ctcgaccac aacaaggag cccactttc catgacatc 
      taccccaag accaacatc agcatcgag ctgcaaccc 
      aaggaacct tcttctaac tgcttctcc ttcacacgt 
      tcagacacc cagcttcgg gaaccttgg gtcaaaggc 
      cagatctac gataaaaca atccggact tttaaagcc 
      tatttgggg ccttcgggt ggccgaagg ggctaccaa 
      gggaccacc ggccaaaca tccacgaca ctggcatgg 
      tacgtcaac gggctgtta gcccccgaa ttgtggctg 
      aggcggggc ttgacctac gccttccgc gtctatggc 
      ggaaacaac ccccacagt gccgagttt tatcacccc 
      ttgattatc accgatgag ccccacgga gggtttgat 
      cggctgtct gacgaagct caggccaaa attagggtt 
      ttggcgggg gtggagtat tccaggagg gggcggcca 
      cgacccact gcagcgggt ccgttgtgt ttagccaaa 
      caccaagac tcgaacgac cgccgtctc gatggtgac 
      tttccaact ttcaagaaa ttcaaccgc tctttggtg 
      tactcctgt gaggaaggc gagccggga gttttggag 
      ttcacgccc aactcgtct tggcccgat attgtttat 
      tacaattcg tttacgcag tcaaacatg ggctggaaa 
      attcacata attgacagc ttttcatcg cgaacgaac 
      ggaatttct aaaatgtgt ggaaacatt ttcgtgtta 
      gtatcgatt ttgtttgtt aagcaattc gtgtaa
    mRNA sequence
    • >TC010653 RA
      tggccgagg tcagcagga gattgtaga aagttagca 
      gcggttcgt gtaactatg tggctccgt tagttgcaa 
      gaaattcga caaaatgaa acggtgctc gtgctgaag 
      atgttacta ctcttcgtt ttgctcttg gcaagttca 
      ggttcatgt caagacagt gaaggcccc taccgtggt 
      aaacacatc gggaagcta aactcgtac caccatgag 
      gtcgggggc gacgtgtac gcggtggac gactacacc 
      ctgctgcta acatcgttt agttatgac gggaacggg 
      gctgacacc ttcttctgg gcgggggcg tccaacagg 
      ccggggccc caggggttc atcgtgccg gacgaatac 
      ggaaagact aatgttttg gcgcgttat ttgcaaaaa 
      gacttcacg ctgacgctc cccgataat aaaaaaatc 
      accgatatt aagtggttt gccatttat gatcttttg 
      agtcaaaac actttcggg gacatttat attccggaa 
      gagttcgaa cctccaact gtacaaaaa ataccacag 
      ttggcctcc aaatcgcat tccgtgtct tcaggcgct 
      gttgaaatc attgacgct aagagaatc cgactgaac 
      gatttccgg tacaatgga caagccaaa agggcgcat 
      ttctgggtc ggagttggc gcccaacct gtgtccaaa 
      gggcacaaa gttcccgac gaaaacgga tatttggac 
      cctctgcgg gcctacaag ggcgagacc atcactctt 
      gagcttccc ggggacttg acaatattc aacatcgcg 
      tggtttagt gttttcgat cttgaaacc aacgaaaat 
      ttgggttct attgttata ccggacgga cttaacgtc 
      cctccgtct ctagtcaaa gttattccg cacgaacac 
      cacctacct aactgtttg caactccat aaagatttc 
      caagtgtcg tgggacatt tttgggcca caaattacc 
      atccaaatg gccggtcaa gtcgatgaa aattcctac 
      atggccttt ggtatgtct gggtcaaag gaacagagt 
      caaatgttg ggttctgat gtcactgtg gcctatttg 
      gacgggtat agaggctac gctaccgac tataacatt 
      acagctttg actccttgt gtcaaagtt ttgggacaa 
      tataaaggt gtgtgcaaa gacgagctt gtagggggg 
      caggacagc aatcaaatg cacacagct acgcgggaa 
      aacggcatc aatattatc acctttcgg cgcgctctt 
      atctcaccc gaccctggc gaccaggaa taccccacc 
      gagggctca atttacata atctgggca atgggccgc 
      ctcgaccac aacaaggag cccactttc catgacatc 
      taccccaag accaacatc agcatcgag ctgcaaccc 
      aaggaacct tcttctaac tgcttctcc ttcacacgt 
      tcagacacc cagcttcgg gaaccttgg gtcaaaggc 
      cagatctac gataaaaca atccggact tttaaagcc 
      tatttgggg ccttcgggt ggccgaagg ggctaccaa 
      gggaccacc ggccaaaca tccacgaca ctggcatgg 
      tacgtcaac gggctgtta gcccccgaa ttgtggctg 
      aggcggggc ttgacctac gccttccgc gtctatggc 
      ggaaacaac ccccacagt gccgagttt tatcacccc 
      ttgattatc accgatgag ccccacgga gggtttgat 
      cggctgtct gacgaagct caggccaaa attagggtt 
      ttggcgggg gtggagtat tccaggagg gggcggcca 
      cgacccact gcagcgggt ccgttgtgt ttagccaaa 
      caccaagac tcgaacgac cgccgtctc gatggtgac 
      tttccaact ttcaagaaa ttcaaccgc tctttggtg 
      tactcctgt gaggaaggc gagccggga gttttggag 
      ttcacgccc aactcgtct tggcccgat attgtttat 
      tacaattcg tttacgcag tcaaacatg ggctggaaa 
      attcacata attgacagc ttttcatcg cgaacgaac 
      ggaatttct aaaatgtgt ggaaacatt ttcgtgtta 
      gtatcgatt ttgtttgtt aagcaattc gtgtaattg 
      tttatttaa acagaggcc ataaacatc agatctatc 
      ttaattttt tggtgattg tgtaaatga ccaggactt 
      aattattta ttgtaatta acacgcctg aactgtaat 
      ttatttata aataaagtg atataatgt ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY