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Gene information
General information
TC010652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8226037..8230035
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010652
    iBeetle screen iB_04888

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010652 RA
      MSRFSGVLQ LTDLDDFIT PSQECIKPV QIEKSKSST 
      GAKITIQDD GSYLQVETD GTTQKLQKV EISLSDCLA 
      CSGCITSAE SVLVTQQSQ EEILRVFEE NTALKNAGK 
      SQEAKLIVV SLSIQPILS IASRYSLPV NDCAAKLVT 
      YFKKLGADM VIDMTVADD FALLESQRE FVRRFRATE 
      SDGVKNVMP MLASSCPGW VCYAEKTHG SYILPYIST 
      TKSPQQIMG SLIKHWVGH NLGGRSIYH VTLMPCYDK 
      KLEAAREDF FDKETQSRD VDCVITAIE LQQMLEKDG 
      CVLENLEKS QFSQPWLLK QDTDVCPNL TRHLGSGSG 
      GYADHIFKY AAKELFGIE VENLEYHNL RNPDFREVV 
      LEKEGKVLL RFAIANGFR NIQNLVQKL KRGKSQYHY 
      VEIMACPSG CLNGGAQIR PKDGTALKE LTTELENLY 
      SSLPVIKPE ENKTVKELY EGWLGGMES DKCTAILHT 
      QYHAVEKTT NALNIKW
    CDS sequence
    • >TC010652 RA
      atgtcgcgg ttcagtgga gtgctccag ttaaccgac 
      ttggacgac tttataaca ccgtcacag gagtgtatc 
      aaacctgtc cagattgag aaaagtaaa agcagtact 
      ggggctaaa atcacaata caagatgat gggtcctat 
      ctacaagtc gaaaccgat gggaccaca caaaagttg 
      caaaaagtt gaaatttcg ttgtctgat tgtttggct 
      tgttccggt tgtataaca tcggctgag agcgtgttg 
      gttacacaa cagagtcaa gaagaaata ttgcgcgtg 
      tttgaggaa aacactgct ttaaaaaat gcggggaaa 
      tcacaggag gccaaatta atcgtagtt tccctctcc 
      attcagcca attctatca atagcaagt cgctacagc 
      ctccctgtt aatgactgt gccgctaaa cttgtaaca 
      tatttcaaa aagttgggc gctgatatg gtcattgat 
      atgaccgta gcggacgat tttgcgcta cttgagagt 
      caaagggag tttgttcgt cgatttcgg gctacggaa 
      agtgacggt gttaaaaac gttatgcct atgttggct 
      tccagttgt ccagggtgg gtctgttac gcggaaaaa 
      acccacggc agttacatt ctcccctac atttctacc 
      accaaatcg ccacaacaa atcatgggg tcattgatc 
      aaacactgg gttggtcat aacttaggg ggccgttct 
      atataccac gtgacactt atgccatgc tacgataaa 
      aaattagaa gccgcacgt gaagatttt tttgataaa 
      gaaacgcaa agccgggac gttgattgc gtcatcact 
      gcaattgaa ctgcaacaa atgctagag aaggatggg 
      tgtgttttg gaaaatttg gaaaagtca caattttca 
      caaccctgg ttattgaaa caagataca gacgtttgt 
      cctaattta actcggcac ttggggtcg ggttcaggg 
      ggttacgct gatcatatt tttaagtac gcagccaag 
      gaattattt gggatcgaa gttgagaat ttggagtat 
      cacaattta aggaatcca gattttcgt gaagtcgtt 
      ttggaaaaa gaaggaaag gttttgttg cggtttgcg 
      attgcgaat ggttttaga aacatacaa aatttggtt 
      cagaaatta aaaaggggc aaatcgcaa tatcactac 
      gttgaaatt atggcttgt ccttcaggg tgtctcaac 
      ggaggggcc caaattcgg ccaaaggac ggcactgca 
      ttaaaagag ttgactacg gaactggaa aacttatac 
      tcgtcactc cctgtcata aaacccgaa gaaaataaa 
      accgtaaaa gagttgtat gagggatgg ttgggaggt 
      atggaaagt gataaatgt acggcgatt ttgcacacg 
      cagtatcac gccgtggaa aaaacgaca aacgctctt 
      aatatcaaa tggtaa
    mRNA sequence
    • >TC010652 RA
      tgttaatta ataaataac aataaaaag gcatttgga 
      cgaattaac gcacgcaaa aaatgtcgc ggttcagtg 
      gagtgctcc agttaaccg acttggacg actttataa 
      caccgtcac aggagtgta tcaaacctg tccagattg 
      agaaaagta aaagcagta ctggggcta aaatcacaa 
      tacaagatg atgggtcct atctacaag tcgaaaccg 
      atgggacca cacaaaagt tgcaaaaag ttgaaattt 
      cgttgtctg attgtttgg cttgttccg gttgtataa 
      catcggctg agagcgtgt tggttacac aacagagtc 
      aagaagaaa tattgcgcg tgtttgagg aaaacactg 
      ctttaaaaa atgcgggga aatcacagg aggccaaat 
      taatcgtag tttccctct ccattcagc caattctat 
      caatagcaa gtcgctaca gcctccctg ttaatgact 
      gtgccgcta aacttgtaa catatttca aaaagttgg 
      gcgctgata tggtcattg atatgaccg tagcggacg 
      attttgcgc tacttgaga gtcaaaggg agtttgttc 
      gtcgatttc gggctacgg aaagtgacg gtgttaaaa 
      acgttatgc ctatgttgg cttccagtt gtccagggt 
      gggtctgtt acgcggaaa aaacccacg gcagttaca 
      ttctcccct acatttcta ccaccaaat cgccacaac 
      aaatcatgg ggtcattga tcaaacact gggttggtc 
      ataacttag ggggccgtt ctatatacc acgtgacac 
      ttatgccat gctacgata aaaaattag aagccgcac 
      gtgaagatt tttttgata aagaaacgc aaagccggg 
      acgttgatt gcgtcatca ctgcaattg aactgcaac 
      aaatgctag agaaggatg ggtgtgttt tggaaaatt 
      tggaaaagt cacaatttt cacaaccct ggttattga 
      aacaagata cagacgttt gtcctaatt taactcggc 
      acttggggt cgggttcag ggggttacg ctgatcata 
      tttttaagt acgcagcca aggaattat ttgggatcg 
      aagttgaga atttggagt atcacaatt taaggaatc 
      cagattttc gtgaagtcg ttttggaaa aagaaggaa 
      aggttttgt tgcggtttg cgattgcga atggtttta 
      gaaacatac aaaatttgg ttcagaaat taaaaaggg 
      gcaaatcgc aatatcact acgttgaaa ttatggctt 
      gtccttcag ggtgtctca acggagggg cccaaattc 
      ggccaaagg acggcactg cattaaaag agttgacta 
      cggaactgg aaaacttat actcgtcac tccctgtca 
      taaaacccg aagaaaata aaaccgtaa aagagttgt 
      atgagggat ggttgggag gtatggaaa gtgataaat 
      gtacggcga ttttgcaca cgcagtatc acgccgtgg 
      aaaaaacga caaacgctc ttaatatca aatggtaat 
      acggtgtaa taattttca ataaactcg ttaattaca 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY