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Gene information
General information
TC010636 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7841947..7852879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010636
    iBeetle screen iB_04884

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010636 RA
      MSHLLKNFP PGPLDLYRR RASFDWKKL KVFLETEDI 
      VKYQNELFE VLRTHPEYQ HDLLSHSFE EQRRIAVRR 
      ALAYADIDL LSVPKIVEN VKTGAVMTR TMMQLSPSS 
      TVKYSVGNG LFTNVIRSQ GTERHYDIL DLADRGEIH 
      GCFCLTEIG HGTNTKGMR TSATYDKNT QQFVLHTPD 
      FEAAKCWAG SLGQMASYA VVYAQLYVE GVHHGLHTF 
      VVPIRDPKT LLPYPGIVV GDMGEKIGL NGIDNGFLI 
      FDNYRIPRE NLLSKNADV TPEGKYVTR FKNPKDRHG 
      AALGALSIG RVSITGICE SYATKALTI AIRYAAVRK 
      QFGPEGGEE VPILEYQSH QHRLLPYLA AIYVLRVFS 
      TFISETNYS FTIETVLNQ NKESLADLG MEIHVISSA 
      CKPVASWLA RDAIQECRE ACGGHGYLK VAGIGDIRN 
      DNDANCTYE GENHVLIQQ TSNWLIKLC SEIERGAKI 
      DTPLQSANF LSNASQILK HKFTANSLD EVYRPEAII 
      DAYQWLVCY LLKQTKDKL DHLTKKEGE FWAKNNTQV 
      YYAKSLAIV FIQHFFLQR MLVVINQAT DADNKRVLS 
      KLFSLYGLW TLEKYLATL YEGGYTQGP KATHLIHEG 
      ILTLCHDIK DDAVSLIDA IAPPDFILN SVLGASDGR 
      VYQRLQSAI FRSPHAMSR PKWWRELVH WNADLKNKL
    CDS sequence
    • >TC010636 RA
      atgtcacat ctattgaaa aattttcct ccaggtccg 
      ttggacctg tataggaga agggcctcg tttgattgg 
      aaaaaattg aaagtgttt ctggaaact gaggatatt 
      gtcaagtac caaaatgag ctgttcgag gtgctgcga 
      acccaccca gagtaccaa cacgaccta ctctcccat 
      tcgttcgag gaacagagg cgtattgca gtgcgtcgg 
      gcccttgcc tacgccgac attgacctc ctatcagtc 
      cccaaaatc gtggaaaat gttaaaact ggggccgtc 
      atgacccgg acaatgatg cagttaagc ccatcctcc 
      accgttaaa tactccgtg ggcaacggg ttgttcacc 
      aacgttatc aggagccaa gggacggag cgccactac 
      gatatctta gacctcgca gaccggggc gagatccat 
      gggtgtttc tgcctgacc gagatcggc catggtacc 
      aacaccaag ggcatgcgg acgagcgcc acttacgac 
      aaaaacacc caacaattc gtcctccac acccctgac 
      ttcgaagcg gccaagtgc tgggccggt tcgctgggt 
      caaatggcc tcctatgcc gtggtctac gcccagctg 
      tacgtcgaa ggggtgcac catgggctc cacacgttc 
      gtggtgccc atcagggac cccaagact ctgctaccg 
      tatcccggg atcgtggtg ggggacatg ggcgagaag 
      atcggcctc aacgggatc gacaatggt tttctgatt 
      tttgataat taccgcatt ccgcgtgag aacctcctg 
      agtaaaaac gccgacgtg acccctgag ggcaagtac 
      gtgacaagg tttaagaac ccgaaggac cgtcacggg 
      gctgcgtta ggggccctg tccataggc cgtgttagc 
      ataactgga atttgcgaa agctatgcc actaaggca 
      ctcacgatt gcgataaga tatgcagct gtgagaaaa 
      cagtttggg ccagagggt ggggaggaa gttccaatt 
      ttggagtat cagtcacat caacaccga cttttgcca 
      tacttggct gcgatttac gtcttgagg gtgtttagc 
      acatttatt tccgaaacg aattatagt ttcaccatt 
      gagacagtt ttgaatcaa aataaggaa tctctggcc 
      gacttgggc atggaaatc cacgtgatt tcctcggcc 
      tgtaagccg gtggcgagt tggttggca cgcgatgcc 
      atccaggag tgcagggag gcttgtggt ggccatgga 
      tacttaaaa gtggccgga attggggat attaggaac 
      gacaacgat gctaactgc acgtacgaa ggcgaaaat 
      cacgtcttg attcaacaa accagcaac tggttgatc 
      aaattatgc tccgagatt gaacgaggg gccaaaatc 
      gacactccg ctacaatcg gccaatttc ctgagcaat 
      gcttcgcaa atactcaaa cacaaattt actgccaat 
      agcctagac gaggtttat cggcctgaa gctatcata 
      gatgcctat cagtggctg gtttgttat cttttgaaa 
      caaacaaag gacaagttg gaccatctg acgaagaaa 
      gaaggcgag ttttgggcc aaaaataac actcaggtg 
      tactacgct aaaagcctg gccattgtc ttcattcag 
      cacttcttc ttgcaacga atgttggtc gttatcaac 
      caagcaacg gacgctgac aacaaaaga gttctctcg 
      aaattattc tccttgtac gggctgtgg accctggag 
      aaatatttg gcaactttg tacgaaggg ggctacacc 
      cagggcccc aaagccact catttgatc cacgaaggc 
      atcttaaca ctctgccac gacattaaa gacgacgca 
      gtgtctcta attgacgct atcgcgccc cccgatttc 
      atcctgaat tcggtgttg ggggcctcg gacggccgc 
      gtttaccag aggctacaa tcggcgatc ttccgctcg 
      ccccacgcc atgagcagg ccgaagtgg tggcgggag 
      ctcgtccat tggaacgcc gatttaaag aataaattg 
      tga
    mRNA sequence
    • >TC010636 RA
      ttacacaat tccagttgt tgatttccg atgtcgact 
      tgaaggtgc ttcacttcg ccacacatt acttacata 
      atcacctcc acttgcttt ctcccaatt agcgaaatt 
      agaaagttt tctacagtt ttctgtaat aattcgata 
      agcatcaag gatcaaggt tatgttagc gtgttttgt 
      aaaaaaaaa ctattaaaa cctgcaagt gtaaagtcc 
      gctacttgt cctcaagtc agtgaacta atagactac 
      atgacagta caaaatttt taatgttat cgcaactgc 
      gagatatat aaattcggt ataaatatt tgtgtttat 
      ctcaaacag tgcgccata ccatcgctg tgaaaatgt 
      cacatctat tgaaaaatt ttcctccag gtccgttgg 
      acctgtata ggagaaggg cctcgtttg attggaaaa 
      aattgaaag tgtttctgg aaactgagg atattgtca 
      agtaccaaa atgagctgt tcgaggtgc tgcgaaccc 
      acccagagt accaacacg acctactct cccattcgt 
      tcgaggaac agaggcgta ttgcagtgc gtcgggccc 
      ttgcctacg ccgacattg acctcctat cagtcccca 
      aaatcgtgg aaaatgtta aaactgggg ccgtcatga 
      cccggacaa tgatgcagt taagcccat cctccaccg 
      ttaaatact ccgtgggca acgggttgt tcaccaacg 
      ttatcagga gccaaggga cggagcgcc actacgata 
      tcttagacc tcgcagacc ggggcgaga tccatgggt 
      gtttctgcc tgaccgaga tcggccatg gtaccaaca 
      ccaagggca tgcggacga gcgccactt acgacaaaa 
      acacccaac aattcgtcc tccacaccc ctgacttcg 
      aagcggcca agtgctggg ccggttcgc tgggtcaaa 
      tggcctcct atgccgtgg tctacgccc agctgtacg 
      tcgaagggg tgcaccatg ggctccaca cgttcgtgg 
      tgcccatca gggacccca agactctgc taccgtatc 
      ccgggatcg tggtggggg acatgggcg agaagatcg 
      gcctcaacg ggatcgaca atggttttc tgatttttg 
      ataattacc gcattccgc gtgagaacc tcctgagta 
      aaaacgccg acgtgaccc ctgagggca agtacgtga 
      caaggttta agaacccga aggaccgtc acggggctg 
      cgttagggg ccctgtcca taggccgtg ttagcataa 
      ctggaattt gcgaaagct atgccacta aggcactca 
      cgattgcga taagatatg cagctgtga gaaaacagt 
      ttgggccag agggtgggg aggaagttc caattttgg 
      agtatcagt cacatcaac accgacttt tgccatact 
      tggctgcga tttacgtct tgagggtgt ttagcacat 
      ttatttccg aaacgaatt atagtttca ccattgaga 
      cagttttga atcaaaata aggaatctc tggccgact 
      tgggcatgg aaatccacg tgatttcct cggcctgta 
      agccggtgg cgagttggt tggcacgcg atgccatcc 
      aggagtgca gggaggctt gtggtggcc atggatact 
      taaaagtgg ccggaattg gggatatta ggaacgaca 
      acgatgcta actgcacgt acgaaggcg aaaatcacg 
      tcttgattc aacaaacca gcaactggt tgatcaaat 
      tatgctccg agattgaac gaggggcca aaatcgaca 
      ctccgctac aatcggcca atttcctga gcaatgctt 
      cgcaaatac tcaaacaca aatttactg ccaatagcc 
      tagacgagg tttatcggc ctgaagcta tcatagatg 
      cctatcagt ggctggttt gttatcttt tgaaacaaa 
      caaaggaca agttggacc atctgacga agaaagaag 
      gcgagtttt gggccaaaa ataacactc aggtgtact 
      acgctaaaa gcctggcca ttgtcttca ttcagcact 
      tcttcttgc aacgaatgt tggtcgtta tcaaccaag 
      caacggacg ctgacaaca aaagagttc tctcgaaat 
      tattctcct tgtacgggc tgtggaccc tggagaaat 
      atttggcaa ctttgtacg aagggggct acacccagg 
      gccccaaag ccactcatt tgatccacg aaggcatct 
      taacactct gccacgaca ttaaagacg acgcagtgt 
      ctctaattg acgctatcg cgccccccg atttcatcc 
      tgaattcgg tgttggggg cctcggacg gccgcgttt 
      accagaggc tacaatcgg cgatcttcc gctcgcccc 
      acgccatga gcaggccga agtggtggc gggagctcg 
      tccattgga acgccgatt taaagaata aattgtgat 
      aagttgggt tgttggttt ttatgttgg ttggaactt 
      tatatattt tatttttgt aacaatata tgaaagaat 
      aaata
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