General information | |||
TC010635 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 7810627..7815198 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC010635 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04883 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
1 larva |
head mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%
legs segment fused - Penetrance (percentage of animals/eggs on slide): more than 80%
legs pretarsus not fused - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
wings mostlydecreased - Number of animals showing the phenotype: 4
oblique dorsal thoracic muscles orientation irregular - Number of animals showing the phenotype: 4
oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 4
oblique dorsal thoracic muscles lateral decreased - Number of animals showing the phenotype: 4
- >TC010635 RA
MVVRQYNQE LEYLEKTSP YCWRIKKGF QPNMNVEGV FYVNQTLEK LMLEELRNA CRPGMAGGF LPGVKQIAN VAALPGIVG KSVGLPDVH SGYGFAIGN MAAFDVDNP ESIVSPGGV GFDINCGVR LLRTNLFEK DVLPVKEQL AQSLFDHIP VGVGSKGII PMNAKDLEE ALEMGMDWS LREGYIWAE DKEHCEEYG RMLNADPSK VSMRAKKRG LPQLGTLGA GNHYAEIQV VDEIYDKWA ACKMGIEEK GQVCVMIHS GSRGFGHQV ATDALVQME KAMKRDKIE TNDRQLACA RISSQEGQD YLKSMAAAA NFAWVNRSS MTFLSRQAF AKQFNMAPD DLDMHVIYD VSHNIAKIE EHIVDGKQK TLLVHRKGS TRAFPPHHP LIPVDYQLT GQPVLIGGT MGTCSYVLT GTQQGMLET FGSTCHGAG RALSRAKSR RNLDYTDVL NKLEEMGIS IRVASPKLV MEEAPESYK NVTDVVNTC HAAGISKKC IKLRPIAVI KG
- >TC010635 RA
atggtggtg cgacagtac aaccaagag ttggaatac ctggaaaag acttccccg tactgctgg cggatcaag aagggtttc cagcccaac atgaacgtg gagggtgtc ttttacgtc aaccaaacc ctggaaaag ctcatgttg gaggagctg cgtaatgcg tgtcgcccc ggaatggct gggggcttc ctgccgggg gtcaagcaa atcgcgaat gttgccgcc ctgcctggc atcgtcggg aagtcggtg gggctccca gatgtccac tctggatac ggctttgct atagggaat atggcagct tttgatgtc gacaacccc gagtccatc gtgtctcca gggggcgtc ggtttcgac attaattgt ggtgtgcga ttgctgcgc acgaatctc tttgaaaag gacgttttg cccgtgaag gaacagttg gcccagagt ttgttcgat catattccc gtgggggtg ggttcgaag gggattatt ccgatgaat gccaaggat ttggaggag gctttggag atggggatg gactggtcg ttgcgtgag gggtacatc tgggctgag gataaggag cattgcgag gagtatgga cggatgctg aacgcggat ccgtccaaa gttagcatg agggccaag aaacggggg cttccgcag cttggtact cttggggcc ggcaaccac tacgccgag atccaggtc gtggacgag atttacgac aaatgggcg gcttgcaag atgggcatc gaggaaaag ggccaagtt tgcgtcatg atccactcc ggtagccgc ggcttcggc caccaagtc gccaccgac gccctcgtc cagatggaa aaagccatg aaacgcgac aaaatcgaa acaaacgac cgccaactg gcctgcgcc cgcatcagc tcacaagaa ggccaagac tacttgaaa tccatggcc gccgcagca aattttgca tgggtcaac cgcagctct atgactttc ttaagtcga caagcgttc gccaaacag ttcaacatg gcccctgat gatttagac atgcacgtg atctacgac gtgtcccac aacattgcc aaaatcgag gaacatatc gttgacggg aagcagaag acgcttttg gtgcacaga aagggctcc acaagggct ttcccgccc caccatccg ctgattccg gttgattat cagttgacg ggccagccg gtcctgatt gggggcact atggggacg tgtagttat gtcttgacg gggacgcag caagggatg ttggagacg tttggctcg acgtgtcat ggagcgggt cgggcgttg tcaagggcc aagtcgagg aggaatttg gattatacc gatgttttg aacaagttg gaggaaatg gggatctcg attagggtt gcgtcaccg aaactcgtg atggaggag gcgccagag tcgtataag aatgtaacg gatgttgtg aatacgtgt cacgcggcc gggattagt aagaagtgt attaagttg aggccgatt gctgttatt aaaggatga
- >TC010635 RA
taacctcaa aaagccggt gttttgaca atccttaat ttcttaaaa aaaagtgga aaaatggtg gtgcgacag tacaaccaa gagttggaa tacctggaa aagacttcc ccgtactgc tggcggatc aagaagggt ttccagccc aacatgaac gtggagggt gtcttttac gtcaaccaa accctggaa aagctcatg ttggaggag ctgcgtaat gcgtgtcgc cccggaatg gctgggggc ttcctgccg ggggtcaag caaatcgcg aatgttgcc gccctgcct ggcatcgtc gggaagtcg gtggggctc ccagatgtc cactctgga tacggcttt gctataggg aatatggca gcttttgat gtcgacaac cccgagtcc atcgtgtct ccagggggc gtcggtttc gacattaat tgtggtgtg cgattgctg cgcacgaat ctctttgaa aaggacgtt ttgcccgtg aaggaacag ttggcccag agtttgttc gatcatatt cccgtgggg gtgggttcg aaggggatt attccgatg aatgccaag gatttggag gaggctttg gagatgggg atggactgg tcgttgcgt gaggggtac atctgggct gaggataag gagcattgc gaggagtat ggacggatg ctgaacgcg gatccgtcc aaagttagc atgagggcc aagaaacgg gggcttccg cagcttggt actcttggg gccggcaac cactacgcc gagatccag gtcgtggac gagatttac gacaaatgg gcggcttgc aagatgggc atcgaggaa aagggccaa gtttgcgtc atgatccac tccggtagc cgcggcttc ggccaccaa gtcgccacc gacgccctc gtccagatg gaaaaagcc atgaaacgc gacaaaatc gaaacaaac gaccgccaa ctggcctgc gcccgcatc agctcacaa gaaggccaa gactacttg aaatccatg gccgccgca gcaaatttt gcatgggtc aaccgcagc tctatgact ttcttaagt cgacaagcg ttcgccaaa cagttcaac atggcccct gatgattta gacatgcac gtgatctac gacgtgtcc cacaacatt gccaaaatc gaggaacat atcgttgac gggaagcag aagacgctt ttggtgcac agaaagggc tccacaagg gctttcccg ccccaccat ccgctgatt ccggttgat tatcagttg acgggccag ccggtcctg attgggggc actatgggg acgtgtagt tatgtcttg acggggacg cagcaaggg atgttggag acgtttggc tcgacgtgt catggagcg ggtcgggcg ttgtcaagg gccaagtcg aggaggaat ttggattat accgatgtt ttgaacaag ttggaggaa atggggatc tcgattagg gttgcgtca ccgaaactc gtgatggag gaggcgcca gagtcgtat aagaatgta acggatgtt gtgaatacg tgtcacgcg gccgggatt agtaagaag tgtattaag ttgaggccg attgctgtt attaaagga tgaagtatt tattaaggt atttatttt aacgtgtaa ataaagatg atggcgttt g
