Status
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Gene information
General information
TC010635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7810627..7815198
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010635
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04883

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    1 larva
    head mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs segment fused - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs pretarsus not fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 4

    wings mostlydecreased - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles orientation irregular - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 4
    oblique dorsal thoracic muscles lateral decreased - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010635 RA
      MVVRQYNQE LEYLEKTSP YCWRIKKGF QPNMNVEGV 
FYVNQTLEK LMLEELRNA CRPGMAGGF LPGVKQIAN 
VAALPGIVG KSVGLPDVH SGYGFAIGN MAAFDVDNP 
ESIVSPGGV GFDINCGVR LLRTNLFEK DVLPVKEQL 
AQSLFDHIP VGVGSKGII PMNAKDLEE ALEMGMDWS 
LREGYIWAE DKEHCEEYG RMLNADPSK VSMRAKKRG 
LPQLGTLGA GNHYAEIQV VDEIYDKWA ACKMGIEEK 
GQVCVMIHS GSRGFGHQV ATDALVQME KAMKRDKIE 
TNDRQLACA RISSQEGQD YLKSMAAAA NFAWVNRSS 
MTFLSRQAF AKQFNMAPD DLDMHVIYD VSHNIAKIE 
EHIVDGKQK TLLVHRKGS TRAFPPHHP LIPVDYQLT 
GQPVLIGGT MGTCSYVLT GTQQGMLET FGSTCHGAG 
RALSRAKSR RNLDYTDVL NKLEEMGIS IRVASPKLV 
MEEAPESYK NVTDVVNTC HAAGISKKC IKLRPIAVI 
KG
    CDS sequence
    • >TC010635 RA
      atggtggtg cgacagtac aaccaagag ttggaatac 
ctggaaaag acttccccg tactgctgg cggatcaag 
aagggtttc cagcccaac atgaacgtg gagggtgtc 
ttttacgtc aaccaaacc ctggaaaag ctcatgttg 
gaggagctg cgtaatgcg tgtcgcccc ggaatggct 
gggggcttc ctgccgggg gtcaagcaa atcgcgaat 
gttgccgcc ctgcctggc atcgtcggg aagtcggtg 
gggctccca gatgtccac tctggatac ggctttgct 
atagggaat atggcagct tttgatgtc gacaacccc 
gagtccatc gtgtctcca gggggcgtc ggtttcgac 
attaattgt ggtgtgcga ttgctgcgc acgaatctc 
tttgaaaag gacgttttg cccgtgaag gaacagttg 
gcccagagt ttgttcgat catattccc gtgggggtg 
ggttcgaag gggattatt ccgatgaat gccaaggat 
ttggaggag gctttggag atggggatg gactggtcg 
ttgcgtgag gggtacatc tgggctgag gataaggag 
cattgcgag gagtatgga cggatgctg aacgcggat 
ccgtccaaa gttagcatg agggccaag aaacggggg 
cttccgcag cttggtact cttggggcc ggcaaccac 
tacgccgag atccaggtc gtggacgag atttacgac 
aaatgggcg gcttgcaag atgggcatc gaggaaaag 
ggccaagtt tgcgtcatg atccactcc ggtagccgc 
ggcttcggc caccaagtc gccaccgac gccctcgtc 
cagatggaa aaagccatg aaacgcgac aaaatcgaa 
acaaacgac cgccaactg gcctgcgcc cgcatcagc 
tcacaagaa ggccaagac tacttgaaa tccatggcc 
gccgcagca aattttgca tgggtcaac cgcagctct 
atgactttc ttaagtcga caagcgttc gccaaacag 
ttcaacatg gcccctgat gatttagac atgcacgtg 
atctacgac gtgtcccac aacattgcc aaaatcgag 
gaacatatc gttgacggg aagcagaag acgcttttg 
gtgcacaga aagggctcc acaagggct ttcccgccc 
caccatccg ctgattccg gttgattat cagttgacg 
ggccagccg gtcctgatt gggggcact atggggacg 
tgtagttat gtcttgacg gggacgcag caagggatg 
ttggagacg tttggctcg acgtgtcat ggagcgggt 
cgggcgttg tcaagggcc aagtcgagg aggaatttg 
gattatacc gatgttttg aacaagttg gaggaaatg 
gggatctcg attagggtt gcgtcaccg aaactcgtg 
atggaggag gcgccagag tcgtataag aatgtaacg 
gatgttgtg aatacgtgt cacgcggcc gggattagt 
aagaagtgt attaagttg aggccgatt gctgttatt 
aaaggatga
    mRNA sequence
    • >TC010635 RA
      taacctcaa aaagccggt gttttgaca atccttaat 
ttcttaaaa aaaagtgga aaaatggtg gtgcgacag 
tacaaccaa gagttggaa tacctggaa aagacttcc 
ccgtactgc tggcggatc aagaagggt ttccagccc 
aacatgaac gtggagggt gtcttttac gtcaaccaa 
accctggaa aagctcatg ttggaggag ctgcgtaat 
gcgtgtcgc cccggaatg gctgggggc ttcctgccg 
ggggtcaag caaatcgcg aatgttgcc gccctgcct 
ggcatcgtc gggaagtcg gtggggctc ccagatgtc 
cactctgga tacggcttt gctataggg aatatggca 
gcttttgat gtcgacaac cccgagtcc atcgtgtct 
ccagggggc gtcggtttc gacattaat tgtggtgtg 
cgattgctg cgcacgaat ctctttgaa aaggacgtt 
ttgcccgtg aaggaacag ttggcccag agtttgttc 
gatcatatt cccgtgggg gtgggttcg aaggggatt 
attccgatg aatgccaag gatttggag gaggctttg 
gagatgggg atggactgg tcgttgcgt gaggggtac 
atctgggct gaggataag gagcattgc gaggagtat 
ggacggatg ctgaacgcg gatccgtcc aaagttagc 
atgagggcc aagaaacgg gggcttccg cagcttggt 
actcttggg gccggcaac cactacgcc gagatccag 
gtcgtggac gagatttac gacaaatgg gcggcttgc 
aagatgggc atcgaggaa aagggccaa gtttgcgtc 
atgatccac tccggtagc cgcggcttc ggccaccaa 
gtcgccacc gacgccctc gtccagatg gaaaaagcc 
atgaaacgc gacaaaatc gaaacaaac gaccgccaa 
ctggcctgc gcccgcatc agctcacaa gaaggccaa 
gactacttg aaatccatg gccgccgca gcaaatttt 
gcatgggtc aaccgcagc tctatgact ttcttaagt 
cgacaagcg ttcgccaaa cagttcaac atggcccct 
gatgattta gacatgcac gtgatctac gacgtgtcc 
cacaacatt gccaaaatc gaggaacat atcgttgac 
gggaagcag aagacgctt ttggtgcac agaaagggc 
tccacaagg gctttcccg ccccaccat ccgctgatt 
ccggttgat tatcagttg acgggccag ccggtcctg 
attgggggc actatgggg acgtgtagt tatgtcttg 
acggggacg cagcaaggg atgttggag acgtttggc 
tcgacgtgt catggagcg ggtcgggcg ttgtcaagg 
gccaagtcg aggaggaat ttggattat accgatgtt 
ttgaacaag ttggaggaa atggggatc tcgattagg 
gttgcgtca ccgaaactc gtgatggag gaggcgcca 
gagtcgtat aagaatgta acggatgtt gtgaatacg 
tgtcacgcg gccgggatt agtaagaag tgtattaag 
ttgaggccg attgctgtt attaaagga tgaagtatt 
tattaaggt atttatttt aacgtgtaa ataaagatg 
atggcgttt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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