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Gene information
General information
TC010635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7810627..7815198
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010635
    iBeetle screen iB_04883

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    1 larva

    head mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs segment fused - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs pretarsus not fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 4

    wings mostlydecreased - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles orientation irregular - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 4
    oblique dorsal thoracic muscles lateral decreased - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010635 RA
      MVVRQYNQE LEYLEKTSP YCWRIKKGF QPNMNVEGV 
      FYVNQTLEK LMLEELRNA CRPGMAGGF LPGVKQIAN 
      VAALPGIVG KSVGLPDVH SGYGFAIGN MAAFDVDNP 
      ESIVSPGGV GFDINCGVR LLRTNLFEK DVLPVKEQL 
      AQSLFDHIP VGVGSKGII PMNAKDLEE ALEMGMDWS 
      LREGYIWAE DKEHCEEYG RMLNADPSK VSMRAKKRG 
      LPQLGTLGA GNHYAEIQV VDEIYDKWA ACKMGIEEK 
      GQVCVMIHS GSRGFGHQV ATDALVQME KAMKRDKIE 
      TNDRQLACA RISSQEGQD YLKSMAAAA NFAWVNRSS 
      MTFLSRQAF AKQFNMAPD DLDMHVIYD VSHNIAKIE 
      EHIVDGKQK TLLVHRKGS TRAFPPHHP LIPVDYQLT 
      GQPVLIGGT MGTCSYVLT GTQQGMLET FGSTCHGAG 
      RALSRAKSR RNLDYTDVL NKLEEMGIS IRVASPKLV 
      MEEAPESYK NVTDVVNTC HAAGISKKC IKLRPIAVI 
      KG
    CDS sequence
    • >TC010635 RA
      atggtggtg cgacagtac aaccaagag ttggaatac 
      ctggaaaag acttccccg tactgctgg cggatcaag 
      aagggtttc cagcccaac atgaacgtg gagggtgtc 
      ttttacgtc aaccaaacc ctggaaaag ctcatgttg 
      gaggagctg cgtaatgcg tgtcgcccc ggaatggct 
      gggggcttc ctgccgggg gtcaagcaa atcgcgaat 
      gttgccgcc ctgcctggc atcgtcggg aagtcggtg 
      gggctccca gatgtccac tctggatac ggctttgct 
      atagggaat atggcagct tttgatgtc gacaacccc 
      gagtccatc gtgtctcca gggggcgtc ggtttcgac 
      attaattgt ggtgtgcga ttgctgcgc acgaatctc 
      tttgaaaag gacgttttg cccgtgaag gaacagttg 
      gcccagagt ttgttcgat catattccc gtgggggtg 
      ggttcgaag gggattatt ccgatgaat gccaaggat 
      ttggaggag gctttggag atggggatg gactggtcg 
      ttgcgtgag gggtacatc tgggctgag gataaggag 
      cattgcgag gagtatgga cggatgctg aacgcggat 
      ccgtccaaa gttagcatg agggccaag aaacggggg 
      cttccgcag cttggtact cttggggcc ggcaaccac 
      tacgccgag atccaggtc gtggacgag atttacgac 
      aaatgggcg gcttgcaag atgggcatc gaggaaaag 
      ggccaagtt tgcgtcatg atccactcc ggtagccgc 
      ggcttcggc caccaagtc gccaccgac gccctcgtc 
      cagatggaa aaagccatg aaacgcgac aaaatcgaa 
      acaaacgac cgccaactg gcctgcgcc cgcatcagc 
      tcacaagaa ggccaagac tacttgaaa tccatggcc 
      gccgcagca aattttgca tgggtcaac cgcagctct 
      atgactttc ttaagtcga caagcgttc gccaaacag 
      ttcaacatg gcccctgat gatttagac atgcacgtg 
      atctacgac gtgtcccac aacattgcc aaaatcgag 
      gaacatatc gttgacggg aagcagaag acgcttttg 
      gtgcacaga aagggctcc acaagggct ttcccgccc 
      caccatccg ctgattccg gttgattat cagttgacg 
      ggccagccg gtcctgatt gggggcact atggggacg 
      tgtagttat gtcttgacg gggacgcag caagggatg 
      ttggagacg tttggctcg acgtgtcat ggagcgggt 
      cgggcgttg tcaagggcc aagtcgagg aggaatttg 
      gattatacc gatgttttg aacaagttg gaggaaatg 
      gggatctcg attagggtt gcgtcaccg aaactcgtg 
      atggaggag gcgccagag tcgtataag aatgtaacg 
      gatgttgtg aatacgtgt cacgcggcc gggattagt 
      aagaagtgt attaagttg aggccgatt gctgttatt 
      aaaggatga
    mRNA sequence
    • >TC010635 RA
      taacctcaa aaagccggt gttttgaca atccttaat 
      ttcttaaaa aaaagtgga aaaatggtg gtgcgacag 
      tacaaccaa gagttggaa tacctggaa aagacttcc 
      ccgtactgc tggcggatc aagaagggt ttccagccc 
      aacatgaac gtggagggt gtcttttac gtcaaccaa 
      accctggaa aagctcatg ttggaggag ctgcgtaat 
      gcgtgtcgc cccggaatg gctgggggc ttcctgccg 
      ggggtcaag caaatcgcg aatgttgcc gccctgcct 
      ggcatcgtc gggaagtcg gtggggctc ccagatgtc 
      cactctgga tacggcttt gctataggg aatatggca 
      gcttttgat gtcgacaac cccgagtcc atcgtgtct 
      ccagggggc gtcggtttc gacattaat tgtggtgtg 
      cgattgctg cgcacgaat ctctttgaa aaggacgtt 
      ttgcccgtg aaggaacag ttggcccag agtttgttc 
      gatcatatt cccgtgggg gtgggttcg aaggggatt 
      attccgatg aatgccaag gatttggag gaggctttg 
      gagatgggg atggactgg tcgttgcgt gaggggtac 
      atctgggct gaggataag gagcattgc gaggagtat 
      ggacggatg ctgaacgcg gatccgtcc aaagttagc 
      atgagggcc aagaaacgg gggcttccg cagcttggt 
      actcttggg gccggcaac cactacgcc gagatccag 
      gtcgtggac gagatttac gacaaatgg gcggcttgc 
      aagatgggc atcgaggaa aagggccaa gtttgcgtc 
      atgatccac tccggtagc cgcggcttc ggccaccaa 
      gtcgccacc gacgccctc gtccagatg gaaaaagcc 
      atgaaacgc gacaaaatc gaaacaaac gaccgccaa 
      ctggcctgc gcccgcatc agctcacaa gaaggccaa 
      gactacttg aaatccatg gccgccgca gcaaatttt 
      gcatgggtc aaccgcagc tctatgact ttcttaagt 
      cgacaagcg ttcgccaaa cagttcaac atggcccct 
      gatgattta gacatgcac gtgatctac gacgtgtcc 
      cacaacatt gccaaaatc gaggaacat atcgttgac 
      gggaagcag aagacgctt ttggtgcac agaaagggc 
      tccacaagg gctttcccg ccccaccat ccgctgatt 
      ccggttgat tatcagttg acgggccag ccggtcctg 
      attgggggc actatgggg acgtgtagt tatgtcttg 
      acggggacg cagcaaggg atgttggag acgtttggc 
      tcgacgtgt catggagcg ggtcgggcg ttgtcaagg 
      gccaagtcg aggaggaat ttggattat accgatgtt 
      ttgaacaag ttggaggaa atggggatc tcgattagg 
      gttgcgtca ccgaaactc gtgatggag gaggcgcca 
      gagtcgtat aagaatgta acggatgtt gtgaatacg 
      tgtcacgcg gccgggatt agtaagaag tgtattaag 
      ttgaggccg attgctgtt attaaagga tgaagtatt 
      tattaaggt atttatttt aacgtgtaa ataaagatg 
      atggcgttt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY