Status
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Gene information
General information
TC031767
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010618,TC010617
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452036.1: 9271..23263
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031767
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04881

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031767 RB
        MNIFVGTPL VQEEFEKLL LAVGGDWEE LFKLGEEDS 
        HPKIVIHSD KLIKPVAAT GRKNLSLLR IQEEENFSD 
        MEEEAGLVA QPVEPEKID EIRRQFEEA AGGMRQEIS 
        SLKVKIKEK LMLLKQNRA KGEKLIRSL QAKIEQIKE 
        PQEGGLITI NLLSPVYPQ VTPANQPEF EEFFNSGCW 
        SDWKSQLEL NQVFLVSED MEQNSERGT EQNTKEQMH 
        KNMGINIIS NKLIRPIGR SRGVKDLKL LREPFIEDV 
        YEEEATQPE MRLQQMVEE VATSFNGER IEEEIRVLK 
        KKITDKAEA LKRNRSFGE DLLRNLDEE EAEIRSNME 
        QKILNEMCK QASLHRHRK RQIIELVTP AYVGTPAGQ 
        EEFEELLLA VGGDWEELF NLGAENDQQ EEDNHPKIV 
        IHSDKVIKP IAATGRKNL SLLRIQEEE NFSDMKEEE 
        EAEAVVQPA EPEKIEELR NQFEEAAGG MREEISALK 
        QDVIECSQL SVKEKQLAN HAVANIVQI VDEDDDSMV 
        TPSSQPDSE LSFSLPFTQ YLAQHGIEN DYSDDISLT 
        LIDQFINED EAASNPVDI IDALSEPWV EVENVINGN 
        VEHPPQPNS PIIEENDVL PERLVVVEA ENNIIRSPS 
        PPPSPMILS QGVNDIQQP SGSEVDVEL NIITPPLPH 
        PPATFDPMS IITQNVIDA LADPWVEER RQGEEMDVE 
        NDIIQPSQL SPILGQIKN RVENRVEGG DVDVENEDN 
        EDDDNIIPP TPPHSRSRR SRRPRKP
      CDS sequence
      • >TC031767 RB
        atgaatatt ttcgtaggc acaccttta gtgcaggaa 
        gaattcgaa aagctcctc ctagctgta gggggagac 
        tgggaagag ctcttcaaa cttggcgaa gaggacagt 
        caccccaaa attgtgatc cactccgac aaattgatt 
        aaaccggtt gcggccacg ggtcgcaaa aaccttagc 
        ctcctaagg attcaagag gaagaaaac ttctccgac 
        atggaggag gaggctggg ctcgtggcg caaccggtg 
        gaaccagaa aaaattgat gaaataagg agacaattt 
        gaagaggcg gctgggggg atgcgacaa gaaatcagt 
        tctctgaaa gttaaaata aaggaaaag ctaatgctt 
        ttaaagcaa aacagagca aagggcgaa aagctcatt 
        agatctctt caagcaaaa attgagcaa ataaaagaa 
        ccccaggag gggggttta attaccatc aacttgctg 
        tcgccagtt tatcctcag gtcactcca gcaaatcag 
        ccagaattt gaagaattt tttaattct ggatgctgg 
        agtgactgg aaaagtcag ctggagttg aatcaagtg 
        ttccttgtg agcgaggac atggaacaa aactctgag 
        cgtggcacg gaacagaac acgaaagag cagatgcat 
        aagaacatg ggaattaac ataatttct aataaactc 
        atcagaccg ataggcagg tcaagagga gtgaaggac 
        ctcaaactc ctgagggaa cccttcatt gaggacgtt 
        tatgaggaa gaagccacc caacctgaa atgaggttg 
        caacaaatg gtggaagaa gttgcaacc tcatttaac 
        ggagaacgt atagaagaa gaaattcgg gtccttaag 
        aaaaaaatt actgataaa gcggaagcg ctcaaacgg 
        aaccgaagc ttcggggag gaccttttg aggaacctc 
        gatgaagaa gaggcagaa attcggtct aatatggag 
        caaaaaata ttaaacgaa atgtgcaag caagccagc 
        ctccaccgc catcgcaaa agacaaatt attgagctc 
        gtaactcct gcctacgta ggcacacct gcagggcag 
        gaagaattc gaagagctc ctcctagct gtagggggt 
        gactgggag gagctcttc aaccttgga gcagaaaac 
        gatcagcaa gaggaggac aatcacccc aaaattgta 
        atacactct gataaagtg attaaaccg attgcggcc 
        acgggtcgc aaaaacctt agcctccta aggattcaa 
        gaggaagaa aacttctcc gatatgaag gaggaggag 
        gaggctgaa gccgtggtg caaccggcg gaaccggaa 
        aaaattgag gaattgagg aaccagttt gaagaggcg 
        gctgggggg atgcgagaa gaaattagt gctctaaaa 
        caggatgtg attgaatgt agtcagtta agtgtgaaa 
        gagaagcaa ttagctaat cacgctgtt gctaacatt 
        gttcaaatc gttgatgag gatgatgat tctatggtt 
        actccttca tcacaacct gatagtgaa ttatcgttt 
        tctttacca tttacgcaa tacctggct caacatggt 
        attgaaaat gattattcg gacgacatc tcgttgacc 
        ttaatagac cagtttatt aacgaagac gaggcggcg 
        tccaatccg gttgacatc attgacgct ttatcggag 
        ccttgggtt gaggtggaa aacgttatc aatggtaac 
        gttgaacat ccccctcaa cccaattca ccgattatt 
        gaggaaaat gatgttctg ccagagaga ttggtggtg 
        gtagaggcc gaaaataac atcattcgg tcaccatca 
        ccaccaccg tcacccatg atattatca cagggcgtc 
        aatgacata caacaacca tctggatcc gaggttgat 
        gtggaactt aacattatc acaccacca cttcctcat 
        cctcctgca acgttcgat ccgatgagt attattact 
        caaaatgtc attgacgca ctggcagac ccgtgggtt 
        gaagagagg aggcagggt gaagagatg gatgttgaa 
        aatgacatc attcaaccc tctcaattg tcacccatt 
        ctagggcaa ataaaaaat agagttgag aatagggtg 
        gagggtgga gacgttgat gtcgaaaac gaagacaat 
        gaggacgac gacaacatc attccaccc actccccct 
        cactcacga tcaaggagg agtcggcgc cctagaaag 
        cctc
      mRNA sequence
      • >TC031767 RB
        aatttttta aatcaaatt tactttaac aaaatattt 
        atcattttt tgcttgatt ctaatattt ttctatttt 
        ttacagcta aaaaggaaa aatttactt tccttaagt 
        aaaactcac ctccaccac cactaagaa aaaatatta 
        aacgaaatg tgcaagcaa gccagcctc taccgccat 
        cgctaaaga gtaagtttt tttaaatta tctataatt 
        aatcttgaa agatacaat tatcgagaa ctatttttg 
        tttattatt atttttctt ttaacttta atgaatatt 
        ttcgtaggc acaccttta gtgcaggaa gaattcgaa 
        aagctcctc ctagctgta gggggagac tgggaagag 
        ctcttcaaa cttggcgaa gaggacagt caccccaaa 
        attgtgatc cactccgac aaattgatt aaaccggtt 
        gcggccacg ggtcgcaaa aaccttagc ctcctaagg 
        attcaagag gaagaaaac ttctccgac atggaggag 
        gaggctggg ctcgtggcg caaccggtg gaaccagaa 
        aaaattgat gaaataagg agacaattt gaagaggcg 
        gctgggggg atgcgacaa gaaatcagt tctctgaaa 
        gttaaaata aaggaaaag ctaatgctt ttaaagcaa 
        aacagagca aagggcgaa aagctcatt agatctctt 
        caagcaaaa attgagcaa ataaaagaa ccccaggag 
        gggggttta attaccatc aacttgctg tcgccagtt 
        tatcctcag gtcactcca gcaaatcag ccagaattt 
        gaagaattt tttaattct ggatgctgg agtgactgg 
        aaaagtcag ctggagttg aatcaagtg ttccttgtg 
        agcgaggac atggaacaa aactctgag cgtggcacg 
        gaacagaac acgaaagag cagatgcat aagaacatg 
        ggaattaac ataatttct aataaactc atcagaccg 
        ataggcagg tcaagagga gtgaaggac ctcaaactc 
        ctgagggaa cccttcatt gaggacgtt tatgaggaa 
        gaagccacc caacctgaa atgaggttg caacaaatg 
        gtggaagaa gttgcaacc tcatttaac ggagaacgt 
        atagaagaa gaaattcgg gtccttaag aaaaaaatt 
        actgataaa gcggaagcg ctcaaacgg aaccgaagc 
        ttcggggag gaccttttg aggaacctc gatgaagaa 
        gaggcagaa attcggtct aatatggag caaaaaata 
        ttaaacgaa atgtgcaag caagccagc ctccaccgc 
        catcgcaaa agacaaatt attgagctc gtaactcct 
        gcctacgta ggcacacct gcagggcag gaagaattc 
        gaagagctc ctcctagct gtagggggt gactgggag 
        gagctcttc aaccttgga gcagaaaac gatcagcaa 
        gaggaggac aatcacccc aaaattgta atacactct 
        gataaagtg attaaaccg attgcggcc acgggtcgc 
        aaaaacctt agcctccta aggattcaa gaggaagaa 
        aacttctcc gatatgaag gaggaggag gaggctgaa 
        gccgtggtg caaccggcg gaaccggaa aaaattgag 
        gaattgagg aaccagttt gaagaggcg gctgggggg 
        atgcgagaa gaaattagt gctctaaaa caggatgtg 
        attgaatgt agtcagtta agtgtgaaa gagaagcaa 
        ttagctaat cacgctgtt gctaacatt gttcaaatc 
        gttgatgag gatgatgat tctatggtt actccttca 
        tcacaacct gatagtgaa ttatcgttt tctttacca 
        tttacgcaa tacctggct caacatggt attgaaaat 
        gattattcg gacgacatc tcgttgacc ttaatagac 
        cagtttatt aacgaagac gaggcggcg tccaatccg 
        gttgacatc attgacgct ttatcggag ccttgggtt 
        gaggtggaa aacgttatc aatggtaac gttgaacat 
        ccccctcaa cccaattca ccgattatt gaggaaaat 
        gatgttctg ccagagaga ttggtggtg gtagaggcc 
        gaaaataac atcattcgg tcaccatca ccaccaccg 
        tcacccatg atattatca cagggcgtc aatgacata 
        caacaacca tctggatcc gaggttgat gtggaactt 
        aacattatc acaccacca cttcctcat cctcctgca 
        acgttcgat ccgatgagt attattact caaaatgtc 
        attgacgca ctggcagac ccgtgggtt gaagagagg 
        aggcagggt gaagagatg gatgttgaa aatgacatc 
        attcaaccc tctcaattg tcacccatt ctagggcaa 
        ataaaaaat agagttgag aatagggtg gagggtgga 
        gacgttgat gtcgaaaac gaagacaat gaggacgac 
        gacaacatc attccaccc actccccct cactcacga 
        tcaaggagg agtcggcgc cctagaaag cctc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY