Status
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Gene information
General information
TC010614 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451776.1: 35555..49429
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010614
    iBeetle screen iB_04880

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010614 RA
      MSNDDYLFA QMSSDTADA KVECTEQPG ENKELTGIR 
      KWVAENLLL LLTLLGVAV GAVVGFGLR PLPPSKSTI 
      ILLSYPGEL FMRILKLII LPLIMSSLI AGSASLNAR 
      LNGRIALRT FLYFLATSV INAVLGVIL AVLVHPGDP 
      GLKADKIES EVQKRSASV LDSLLDLGR NVVADNIFQ 
      ATFQQAHTD YVNVTERVF NETSGVFYT IDVTMAVIK 
      YRSGTNTIG IVVFCLAFG TILGTLGQK AKIVIEFFS 
      VIFDVVMKM VTGVIWLTP IGVSSVIAG KILSVNNIA 
      LVMAQLGWF IATVIIGVF IYQLVIMQA IYCLFLRKN 
      PFKFYLGLV QGTLTAFAT ASTAAALPV TFTLMEDKL 
      KIDSRITRF VLPIGCNIN MDGTALFVA VASVFFAQT 
      NSIYLGVGE LITVCFAST AASFSSASV PSAALVLIL 
      MVLTAIDIP DQDVFLLFA VDWLVDRFR TTNNMLGDC 
      YAAAVVEKL SKKELMACD AILFQESSS FDNNLQKDF 
      EPSDIVIDR GLNNGATK
    • >TC010614 RB
      MSSDTADAK VECTEQPGE NKELTGIRK WVAENLLLL 
      LTLLGVAVG AVVGFGLRP LPPSKSTII LLSYPGELF 
      MRILKLIIL PLIMSSLIA GSASLNARL NGRIALRTF 
      LYFLATSVI NAVLGVILA VLVHPGDPG LKADKIESE 
      VQKRSASVL DSLLDLGRN VVADNIFQA TFQQAHTDY 
      VNVTERVFN ETSGVFYTI DVTMAVIKY RSGTNTIGI 
      VVFCLAFGT ILGTLGQKA KIVIEFFSV IFDVVMKMV 
      TGVIWLTPI GVSSVIAGK ILSVNNIAL VMAQLGWFI 
      ATVIIGVFI YQLVIMQAI YCLFLRKNP FKFYLGLVQ 
      GTLTAFATA STAAALPVT FTLMEDKLK IDSRITRFV 
      LPIGCNINM DGTALFVAV ASVFFAQTN SIYLGVGEL 
      ITVCFASTA ASFSSASVP SAALVLILM VLTAIDIPD 
      QDVFLLFAV DWLVDRFRT TNNMLGDCY AAAVVEKLS 
      KKELMACDA ILFQESSSF DNNLQKDFE PSDIVIDRG 
      LNNGATK
    CDS sequence
    • >TC010614 RA
      atgagcaac gatgactat ttatttgcc caaatgtca 
      tccgacact gccgatgca aaagtggaa tgtacagaa 
      cagccaggt gaaaacaaa gagttgacc gggatcagg 
      aaatgggtc gcggaaaac ttgctcctt ctactgact 
      ctcctagga gttgccgtt ggcgctgtt gtcggcttc 
      ggcctccgt ccgctgcct ccatcaaag agcacaata 
      atcctgctg tcgtatccc ggggagctg ttcatgcgg 
      atcctcaag ctgatcatt ctgccgctg atcatgtcc 
      agcctgatc gcggggagc gcgagcctg aacgcgcgc 
      ctcaacggg cgcatcgcc ctcaggacc ttcctgtac 
      ttcctggcc acgtcggtg attaacgcg gtgctgggc 
      gtgatcctg gccgtgctc gtccatcca ggtgatccc 
      ggactcaaa gccgacaaa atcgagtcg gaagtgcag 
      aaacggagc gcttccgtc ttagatagc ttattggac 
      ttgggcaga aacgtcgtc gccgataat atttttcaa 
      gccactttt cagcaagcc cacactgat tacgtcaat 
      gtgaccgaa agagtgttt aacgaaaca agcggcgta 
      ttttatacc attgacgtc acaatggct gtaatcaaa 
      tacagatca ggaaccaac acgataggc atcgtcgtt 
      ttctgcctg gccttcggc acaatcctt ggcactctg 
      ggccagaag gccaagatc gtcatcgag tttttctcc 
      gtgattttc gacgtggtg atgaagatg gtcaccggc 
      gtcatctgg ctgactccc atcggggtc agcagcgtc 
      atcgcgggc aagatcctc tccgtcaac aacatcgcg 
      ctggtgatg gcccagctg ggctggttc atcgccact 
      gtcatcatc ggcgtcttc atctatcag ctcgtgatc 
      atgcaggcg atttactgc ttattcttg cgcaaaaac 
      ccgttcaag ttctacttg ggcctggtc caagggacc 
      ctaacggcc tttgctaca gcttccaca gctgccgcg 
      cttcctgtc accttcact ctcatggaa gataagctc 
      aaaatcgat tccagaatc acgaggttt gttttgccg 
      attggctgc aatattaac atggacggg acggcgctc 
      ttcgtcgcc gtggcctcg gtgtttttc gcccaaacc 
      aatagtatt tatctagga gttggggaa ctcatcact 
      gtttgtttt gcatcaacg gcggcgtcg ttttcctcc 
      gctagtgtc cctagtgcc gccctggtg ctcattctg 
      atggttctc acagctatt gacattcct gatcaagat 
      gtattccta ctgtttgcc gttgattgg cttgtagat 
      cgattccgt accaccaat aatatgtta ggtgattgt 
      tatgcggcc gctgttgtt gaaaaactg tcgaaaaaa 
      gaattgatg gcttgcgac gcaattctt ttccaggaa 
      tcgagcagt ttcgataat aacctacaa aaagacttc 
      gaacctagt gatattgtt atagatcgt ggactaaat 
      aatggtgct acaaagtaa
    • >TC010614 RB
      atgtcatcc gacactgcc gatgcaaaa gtggaatgt 
      acagaacag ccaggtgaa aacaaagag ttgaccggg 
      atcaggaaa tgggtcgcg gaaaacttg ctccttcta 
      ctgactctc ctaggagtt gccgttggc gctgttgtc 
      ggcttcggc ctccgtccg ctgcctcca tcaaagagc 
      acaataatc ctgctgtcg tatcccggg gagctgttc 
      atgcggatc ctcaagctg atcattctg ccgctgatc 
      atgtccagc ctgatcgcg gggagcgcg agcctgaac 
      gcgcgcctc aacgggcgc atcgccctc aggaccttc 
      ctgtacttc ctggccacg tcggtgatt aacgcggtg 
      ctgggcgtg atcctggcc gtgctcgtc catccaggt 
      gatcccgga ctcaaagcc gacaaaatc gagtcggaa 
      gtgcagaaa cggagcgct tccgtctta gatagctta 
      ttggacttg ggcagaaac gtcgtcgcc gataatatt 
      tttcaagcc acttttcag caagcccac actgattac 
      gtcaatgtg accgaaaga gtgtttaac gaaacaagc 
      ggcgtattt tataccatt gacgtcaca atggctgta 
      atcaaatac agatcagga accaacacg ataggcatc 
      gtcgttttc tgcctggcc ttcggcaca atccttggc 
      actctgggc cagaaggcc aagatcgtc atcgagttt 
      ttctccgtg attttcgac gtggtgatg aagatggtc 
      accggcgtc atctggctg actcccatc ggggtcagc 
      agcgtcatc gcgggcaag atcctctcc gtcaacaac 
      atcgcgctg gtgatggcc cagctgggc tggttcatc 
      gccactgtc atcatcggc gtcttcatc tatcagctc 
      gtgatcatg caggcgatt tactgctta ttcttgcgc 
      aaaaacccg ttcaagttc tacttgggc ctggtccaa 
      gggacccta acggccttt gctacagct tccacagct 
      gccgcgctt cctgtcacc ttcactctc atggaagat 
      aagctcaaa atcgattcc agaatcacg aggtttgtt 
      ttgccgatt ggctgcaat attaacatg gacgggacg 
      gcgctcttc gtcgccgtg gcctcggtg tttttcgcc 
      caaaccaat agtatttat ctaggagtt ggggaactc 
      atcactgtt tgttttgca tcaacggcg gcgtcgttt 
      tcctccgct agtgtccct agtgccgcc ctggtgctc 
      attctgatg gttctcaca gctattgac attcctgat 
      caagatgta ttcctactg tttgccgtt gattggctt 
      gtagatcga ttccgtacc accaataat atgttaggt 
      gattgttat gcggccgct gttgttgaa aaactgtcg 
      aaaaaagaa ttgatggct tgcgacgca attcttttc 
      caggaatcg agcagtttc gataataac ctacaaaaa 
      gacttcgaa cctagtgat attgttata gatcgtgga 
      ctaaataat ggtgctaca aagtaa
    mRNA sequence
    • >TC010614 RA
      cagttgtgc agttacgtt ttgaatcct ttgcagata 
      ggtccagtc gctttctag cttaaattt tcctttgct 
      ttcaccact aaagtgtcg ctgataaaa aggagtttc 
      aaagattaa caaaaacgg ctaacagac aagtttttc 
      gtttactgg ccactccac acgtggaat ggaagaaaa 
      gtcttcgag attttcggg taacgattt gtttctctt 
      tcatcaatt tgtaatgtt aattgatga ccggctcaa 
      tcaaacgtt attagcgag gcgcgactc gctttattg 
      ctataaaag cgatttatt agtcgtgtc aacagttgt 
      ggctgtgca acatgagca acgatgact atttatttg 
      cccaaatgt catccgaca ctgccgatg caaaagtgg 
      aatgtacag aacagccag gtgaaaaca aagagttga 
      ccgggatca ggaaatggg tcgcggaaa acttgctcc 
      ttctactga ctctcctag gagttgccg ttggcgctg 
      ttgtcggct tcggcctcc gtccgctgc ctccatcaa 
      agagcacaa taatcctgc tgtcgtatc ccggggagc 
      tgttcatgc ggatcctca agctgatca ttctgccgc 
      tgatcatgt ccagcctga tcgcgggga gcgcgagcc 
      tgaacgcgc gcctcaacg ggcgcatcg ccctcagga 
      ccttcctgt acttcctgg ccacgtcgg tgattaacg 
      cggtgctgg gcgtgatcc tggccgtgc tcgtccatc 
      caggtgatc ccggactca aagccgaca aaatcgagt 
      cggaagtgc agaaacgga gcgcttccg tcttagata 
      gcttattgg acttgggca gaaacgtcg tcgccgata 
      atatttttc aagccactt ttcagcaag cccacactg 
      attacgtca atgtgaccg aaagagtgt ttaacgaaa 
      caagcggcg tattttata ccattgacg tcacaatgg 
      ctgtaatca aatacagat caggaacca acacgatag 
      gcatcgtcg ttttctgcc tggccttcg gcacaatcc 
      ttggcactc tgggccaga aggccaaga tcgtcatcg 
      agtttttct ccgtgattt tcgacgtgg tgatgaaga 
      tggtcaccg gcgtcatct ggctgactc ccatcgggg 
      tcagcagcg tcatcgcgg gcaagatcc tctccgtca 
      acaacatcg cgctggtga tggcccagc tgggctggt 
      tcatcgcca ctgtcatca tcggcgtct tcatctatc 
      agctcgtga tcatgcagg cgatttact gcttattct 
      tgcgcaaaa acccgttca agttctact tgggcctgg 
      tccaaggga ccctaacgg cctttgcta cagcttcca 
      cagctgccg cgcttcctg tcaccttca ctctcatgg 
      aagataagc tcaaaatcg attccagaa tcacgaggt 
      ttgttttgc cgattggct gcaatatta acatggacg 
      ggacggcgc tcttcgtcg ccgtggcct cggtgtttt 
      tcgcccaaa ccaatagta tttatctag gagttgggg 
      aactcatca ctgtttgtt ttgcatcaa cggcggcgt 
      cgttttcct ccgctagtg tccctagtg ccgccctgg 
      tgctcattc tgatggttc tcacagcta ttgacattc 
      ctgatcaag atgtattcc tactgtttg ccgttgatt 
      ggcttgtag atcgattcc gtaccacca ataatatgt 
      taggtgatt gttatgcgg ccgctgttg ttgaaaaac 
      tgtcgaaaa aagaattga tggcttgcg acgcaattc 
      ttttccagg aatcgagca gtttcgata ataacctac 
      aaaaagact tcgaaccta gtgatattg ttatagatc 
      gtggactaa ataatggtg ctacaaagt aatgcaata 
      tttcaacaa ccctaaatt aatttgtga tgctagttt 
      gtacacacc atagtttag gatttagct caatgaata 
      aacatattt atgtata
    • >TC010614 RB
      aacaatttg tgaaagtca cgttggatt attgaattg 
      aaaaacaca accggtttg ctcagtgtg gttttagca 
      aattgccga ttaggaatt attggttac atgacacga 
      ctatttatt tgcccaaat gtcatccga cactgccga 
      tgcaaaagt ggaatgtac agaacagcc aggtgaaaa 
      caaagagtt gaccgggat caggaaatg ggtcgcgga 
      aaacttgct ccttctact gactctcct aggagttgc 
      cgttggcgc tgttgtcgg cttcggcct ccgtccgct 
      gcctccatc aaagagcac aataatcct gctgtcgta 
      tcccgggga gctgttcat gcggatcct caagctgat 
      cattctgcc gctgatcat gtccagcct gatcgcggg 
      gagcgcgag cctgaacgc gcgcctcaa cgggcgcat 
      cgccctcag gaccttcct gtacttcct ggccacgtc 
      ggtgattaa cgcggtgct gggcgtgat cctggccgt 
      gctcgtcca tccaggtga tcccggact caaagccga 
      caaaatcga gtcggaagt gcagaaacg gagcgcttc 
      cgtcttaga tagcttatt ggacttggg cagaaacgt 
      cgtcgccga taatatttt tcaagccac ttttcagca 
      agcccacac tgattacgt caatgtgac cgaaagagt 
      gtttaacga aacaagcgg cgtatttta taccattga 
      cgtcacaat ggctgtaat caaatacag atcaggaac 
      caacacgat aggcatcgt cgttttctg cctggcctt 
      cggcacaat ccttggcac tctgggcca gaaggccaa 
      gatcgtcat cgagttttt ctccgtgat tttcgacgt 
      ggtgatgaa gatggtcac cggcgtcat ctggctgac 
      tcccatcgg ggtcagcag cgtcatcgc gggcaagat 
      cctctccgt caacaacat cgcgctggt gatggccca 
      gctgggctg gttcatcgc cactgtcat catcggcgt 
      cttcatcta tcagctcgt gatcatgca ggcgattta 
      ctgcttatt cttgcgcaa aaacccgtt caagttcta 
      cttgggcct ggtccaagg gaccctaac ggcctttgc 
      tacagcttc cacagctgc cgcgcttcc tgtcacctt 
      cactctcat ggaagataa gctcaaaat cgattccag 
      aatcacgag gtttgtttt gccgattgg ctgcaatat 
      taacatgga cgggacggc gctcttcgt cgccgtggc 
      ctcggtgtt tttcgccca aaccaatag tatttatct 
      aggagttgg ggaactcat cactgtttg ttttgcatc 
      aacggcggc gtcgttttc ctccgctag tgtccctag 
      tgccgccct ggtgctcat tctgatggt tctcacagc 
      tattgacat tcctgatca agatgtatt cctactgtt 
      tgccgttga ttggcttgt agatcgatt ccgtaccac 
      caataatat gttaggtga ttgttatgc ggccgctgt 
      tgttgaaaa actgtcgaa aaaagaatt gatggcttg 
      cgacgcaat tcttttcca ggaatcgag cagtttcga 
      taataacct acaaaaaga cttcgaacc tagtgatat 
      tgttataga tcgtggact aaataatgg tgctacaaa 
      gtaatgcaa tatttcaac aaccctaaa ttaatttgt 
      gatgctagt ttgtacaca ccatagttt aggatttag 
      ctcaatgaa taaacatat ttatgtata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY