| General information | |||
| TC010596 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 5221232..5251166 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010596 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04877 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC010596 RA
MMSSEEETE FGLYTDKLL KKTKRTRQR VDAGEPRNS YSSIPNFSS RPTFLGGGN NLYGAIFTQ QQQFGLFGP GFAPAKMLN ELLGRQKAE GGESGEEGK FDAVIRCDG SPPSGEQLA HHMLRDILQ GRKHELLAL EHDMRNGQD HIINNNNNE PKAEPERND VEAAAAMKE CLAEAGVGD RQQAELMEE ALNGMETES LPSPKVEPS SPAKPEDAK KARVENIVS GMRSSPAPP QVNGCKKRK LYQPQQHDS NAERYIDDE EDESAEPIR QKRVEKNLL KSQLRTMQE QLAEMQQKY MQLCTRVEQ GSEESQEIE EIPSDSENS KRSAPPSPI TTSNTPNQS TPSLAQQVS KLKISPPGP PFHNGMLHH DHQHMSSAA AMYLGVSHK FLMEQEARF AKEAAVAAM NSESSVQQS QHQSHHPSQ KPENIADRL HIMRNMTPT GTDLEGLAD ALKTEITSS LSNLIDSIL SRFVHQRRC KQTEAATAA EQLNKDLMM ASQLLDRKS PRTKVVERP QAPPPPPNS QMVNGTSGC PPMMSVHMN NMQKPNDLQ IRPPMFQPP KPPAISQAP LFGMSHPFC VSKQENPEQ NEALSLVVT PKKKRHKVT DTRITPRTV SRILGQDGI GMSPAAMEP NTCSSPRPP PYHQPPPML PVSLPTSVA IPNPGLHES QVFSPYSPF FHGPSHGHH MAMQASSSP PRMHKIERE SPPIHHPPT LLHPALLAA AQHGGSPDY GQLRAPSGG MNMDNGDRN SDCNSGDIT YDGMQPTIS FLCKDFSKN LSNHHTNIS LTPEHSSTL TPMHLRKAK LMFFWVRYP SSAVLKMYF PDIKFNKNN TAQLVKWFS NFREFYYIQ MEKYARQAV SEGVKNAED LHVGGDSEL YRVLNLHYN RNNHIEVPS NFRFVVEQT LREFFKAIQ EGRDAEQSW KKSIYKIIS RLDDPVPEY FKSPNFLEQ LE
- >TC010596 RA
atgatgtca tcggaggag gagactgaa ttcggtttg tacacagat aagctcctg aagaaaacc aaaagaact agacagcgt gtagacgcc ggtgagccg cgtaactcc tactcgtcg attccgaat ttcagttcg cgtccgact tttctcggt ggcggtaac aatctgtac ggcgcaatt ttcacccaa cagcagcag ttcggtctg ttcggaccc gggttcgcg cccgcaaag atgctcaac gagctgctg gggcggcag aaggccgaa ggcggcgaa agcggcgaa gagggcaag ttcgacgcg gtgatccgc tgcgacggc agcccgccc tcgggtgaa caactggcc catcacatg ctccgggac atcctgcag ggccgcaag cacgagctt ttggccttg gaacacgac atgcgcaac ggccaggac cacatcatc aacaacaac aacaacgaa cccaaggcg gaaccagaa cgcaacgac gtcgaagcc gccgccgcg atgaaggag tgcctcgct gaggccgga gtcggcgac cgccagcag gccgagctc atggaggag gcgctcaat gggatggag accgagtcg ctgccctcg ccaaaggtg gagccctcg agtccggcc aagccagag gatgcgaag aaagcgcgc gtcgaaaac attgtctca ggtatgcgg tcgagtccg gcgcctccg caagtcaac ggctgcaag aaacgaaag ctctaccaa ccccagcaa cacgacagt aacgctgaa cggtatatt gacgatgaa gaagatgaa agtgcggaa ccaatcaga caaaaacgc gtcgagaaa aatttactc aaatcgcag ctgagaacg atgcaagaa cagttggcg gaaatgcag cagaaatac atgcagttg tgcacgcgc gttgaacaa gggtctgaa gaaagtcaa gaaattgaa gaaattccc agtgatagt gaaaacagt aaacgatca gcgcctcct tcgcccata accaccagt aatacccct aatcaatcg acgccaagc ttagcacaa caggtgagc aaacttaaa ataagccct ccaggacct cctttccac aatggtatg ttacatcat gatcaccag cacatgagc agtgccgcc gccatgtac ttgggcgtg agccacaaa ttcctgatg gagcaggaa gcccgtttt gctaaagaa gcagcagtg gcagcaatg aacagcgaa tcatcagtc caacaatcc cagcatcag tctcatcat ccatcgcaa aaacccgaa aatatagcc gacagatta cacattatg agaaacatg acaccgacc ggtaccgac ttggaaggc ctcgccgac gctcttaaa accgaaatc acgtcgtct ctttcaaat ttgatcgac tcgatacta tcaaggttc gtccaccaa cggagatgc aaacagacg gaggcggcg acagccgcc gaacaactc aacaaagac ttgatgatg gcctcgcaa ttactcgac aggaaatcg ccgcggacc aaagtagta gaacggcca caagcgccg ccgccgccg cccaacagc cagatggtg aacggcacg agcggctgc cctccgatg atgtccgtt cacatgaac aacatgcaa aaacccaac gacctccaa ataaggcct ccgatgttc cagcccccg aaacccccg gcaatcagc caagcccct ctttttgga atgtcacac ccgttttgc gtgtccaag caagaaaac cccgagcag aacgaagcg ctcagcctc gtagtaact cctaagaaa aagcggcac aaggtgacg gacacccgg ataacgccc aggaccgtg agccgcatc ctgggccag gacggcatc ggcatgtcg cccgccgcc atggagccc aacacgtgc agttccccg cgcccgccc ccatatcac cagccgccg ccgatgttg cccgtctcc cttccgacc tcggtggcg atccccaac cccggactg cacgaatct caggtcttc tcgccctac agccccttc tttcatgga ccaagtcac ggacaccac atggcgatg caggcatcg tcatcacca cctcgcatg cacaaaatc gagcgagag tcgcctcca atccaccac cccccgacg cttctgcac cccgctctc ctggctgcg gcccaacat gggggctcc cccgactac ggacagcta agggcgccg tccgggggc atgaacatg gacaacggc gatagaaat tctgattgt aactcaggg gacatcacc tacgatgga atgcagcct actatatcc tttttatgc aaagatttt agtaaaaat cttagtaac caccataca aatatttcc ttaactcct gagcactcg tcaacactg acaccgatg catctcagg aaagcgaaa ttaatgttc ttctgggtt cggtatccg agctcagca gtactaaaa atgtatttc cccgatatc aagtttaac aagaacaac actgcccag ctcgtcaag tggttctcg aacttcagg gagttctat tacatccag atggagaag tacgccaga caggcagtt tccgaaggt gtgaagaac gctgaagat ctgcatgtc ggtggtgat tccgaatta taccgggtg ctcaatttg cactacaac agaaacaat cacatcgag gttccttca aacttccgg ttcgtcgtg gagcagacg ctccgggag tttttcaag gccatccag gagggccgg gatgccgag cagtcatgg aagaagtcc atctacaag atcatctcg cgactggac gacccggtc ccggagtac ttcaagtcc cccaatttc ctggagcaa ttggagtga
- >TC010596 RA
agtaaatgg aatcaaact tatcaatac caaccctcc ctttcaaat aaaaggtat tgacatggg cgactattc ttaaagtgg ggacgacta tctggaggg gggcacaga acgaccaat aacgctgga acttatgat gtcatcgga ggaggagac tgaattcgg tttgtacac agataagct cctgaagaa aaccaaaag aactagaca gcgtgtaga cgccggtga gccgcgtaa ctcctactc gtcgattcc gaatttcag ttcgcgtcc gacttttct cggtggcgg taacaatct gtacggcgc aattttcac ccaacagca gcagttcgg tctgttcgg acccgggtt cgcgcccgc aaagatgct caacgagct gctggggcg gcagaaggc cgaaggcgg cgaaagcgg cgaagaggg caagttcga cgcggtgat ccgctgcga cggcagccc gccctcggg tgaacaact ggcccatca catgctccg ggacatcct gcagggccg caagcacga gcttttggc cttggaaca cgacatgcg caacggcca ggaccacat catcaacaa caacaacaa cgaacccaa ggcggaacc agaacgcaa cgacgtcga agccgccgc cgcgatgaa ggagtgcct cgctgaggc cggagtcgg cgaccgcca gcaggccga gctcatgga ggaggcgct caatgggat ggagaccga gtcgctgcc ctcgccaaa ggtggagcc ctcgagtcc ggccaagcc agaggatgc gaagaaagc gcgcgtcga aaacattgt ctcaggtat gcggtcgag tccggcgcc tccgcaagt caacggctg caagaaacg aaagctcta ccaacccca gcaacacga cagtaacgc tgaacggta tattgacga tgaagaaga tgaaagtgc ggaaccaat cagacaaaa acgcgtcga gaaaaattt actcaaatc gcagctgag aacgatgca agaacagtt ggcggaaat gcagcagaa atacatgca gttgtgcac gcgcgttga acaagggtc tgaagaaag tcaagaaat tgaagaaat tcccagtga tagtgaaaa cagtaaacg atcagcgcc tccttcgcc cataaccac cagtaatac ccctaatca atcgacgcc aagcttagc acaacaggt gagcaaact taaaataag ccctccagg acctccttt ccacaatgg tatgttaca tcatgatca ccagcacat gagcagtgc cgccgccat gtacttggg cgtgagcca caaattcct gatggagca ggaagcccg ttttgctaa agaagcagc agtggcagc aatgaacag cgaatcatc agtccaaca atcccagca tcagtctca tcatccatc gcaaaaacc cgaaaatat agccgacag attacacat tatgagaaa catgacacc gaccggtac cgacttgga aggcctcgc cgacgctct taaaaccga aatcacgtc gtctctttc aaatttgat cgactcgat actatcaag gttcgtcca ccaacggag atgcaaaca gacggaggc ggcgacagc cgccgaaca actcaacaa agacttgat gatggcctc gcaattact cgacaggaa atcgccgcg gaccaaagt agtagaacg gccacaagc gccgccgcc gccgcccaa cagccagat ggtgaacgg cacgagcgg ctgccctcc gatgatgtc cgttcacat gaacaacat gcaaaaacc caacgacct ccaaataag gcctccgat gttccagcc cccgaaacc cccggcaat cagccaagc ccctctttt tggaatgtc acacccgtt ttgcgtgtc caagcaaga aaaccccga gcagaacga agcgctcag cctcgtagt aactcctaa gaaaaagcg gcacaaggt gacggacac ccggataac gcccaggac cgtgagccg catcctggg ccaggacgg catcggcat gtcgcccgc cgccatgga gcccaacac gtgcagttc cccgcgccc gcccccata tcaccagcc gccgccgat gttgcccgt ctcccttcc gacctcggt ggcgatccc caaccccgg actgcacga atctcaggt cttctcgcc ctacagccc cttctttca tggaccaag tcacggaca ccacatggc gatgcaggc atcgtcatc accacctcg catgcacaa aatcgagcg agagtcgcc tccaatcca ccacccccc gacgcttct gcaccccgc tctcctggc tgcggccca acatggggg ctcccccga ctacggaca gctaagggc gccgtccgg gggcatgaa catggacaa cggcgatag aaattctga ttgtaactc aggggacat cacctacga tggaatgca gcctactat atccttttt atgcaaaga ttttagtaa aaatcttag taaccacca tacaaatat ttccttaac tcctgagca ctcgtcaac actgacacc gatgcatct caggaaagc gaaattaat gttcttctg ggttcggta tccgagctc agcagtact aaaaatgta tttccccga tatcaagtt taacaagaa caacactgc ccagctcgt caagtggtt ctcgaactt cagggagtt ctattacat ccagatgga gaagtacgc cagacaggc agtttccga aggtgtgaa gaacgctga agatctgca tgtcggtgg tgattccga attataccg ggtgctcaa tttgcacta caacagaaa caatcacat cgaggttcc ttcaaactt ccggttcgt cgtggagca gacgctccg ggagttttt caaggccat ccaggaggg ccgggatgc cgagcagtc atggaagaa gtccatcta caagatcat ctcgcgact ggacgaccc ggtcccgga gtacttcaa gtcccccaa tttcctgga gcaattgga gtgaccggt tggccggac cgagaagcc ggtatccag cggtagcca gtggctacc gtgcggtac catccgtta gacacggca aaaacacca gagagaatc ggtgacacg tgacaaaac tgtcaacag tttatatgt tgttttgcc gggaatgag cttggagga cgccgcttt gacgccatc ttcgaaact tgttccgga cactcttgt taaatagtt gtgtttagt tgacagaaa gacaatttt atcgcctca ggctttatg ttcgttcaa acgaacagt gaacacaaa tcaatcaat tttccagtg attacctga atatttacc ggatagaat tttatgacg tcaagtctt tcttttctt ttcaaccag cagtcatca agttcaaaa tctgcactc tcattgcgt tacttctgg cattattat gttcggtgc cactgtaaa ctgcccctt ttacccctg tatataaag cgacgtgta catagtatt tatcactta attttattt atatcttgt attattatt atcatctca accaaagtt tttatttta ctagtccca cataagatt attcccttt tgaataatt ttacgtaaa ctatcagtg ccaaacttt gaacacaat acaacgctt caaagtttc gcactgaaa aaagattta ttgcagcaa ttattcttc cattttgta tcaaataca actcgaaag tctaattcc tgtgattta ttcggtatt aagtaggta tgccttgtt ttgtattta ttattttgt accccaaca ctcccccat tctgtttta atttattag ttgtagact agttgctat tattatttc tgttagcat acctaccta attacctgt tttatttta tattttagt taattttaa tcacttgca caatcaaat agtgtaggt tctacacga tggcaaata cagtgtgag gcaactagt cagtaaaaa taaaatgta ttatcttta tttggtttt attttggtt tgtttcttt ttggttcga agacagttt acagtgtga tgtttcgag tacaataat tttgagcca taaatcgta aattgtaca taaatttgg agctcatgg tttccttca catcccgca atagttgag gtcacatac acaatcgaa ggccccgag agcccttaa cttagcctt atggtgagg ctaggaggt cgtgatttc gctgttcct aaaatccta tattcgaga tactgccga aattgtaag actgccatt cacttaaga ttgatttag cggtttcgt tgtcgtttc gcgtttatt tgcgtttca aaattaatc gtttggatg tttgtgacg ttgcgttga tttttcggt ttgttctcg gggcctgca tcggtacat tacataaga aaaataaaa acaaaattt aaaaattag attaatcta aatagttat ttattaaga tttaaagta tataacgtt gtcgactgt aaagtaccc gtaatgctg atggttttc aaacaagca aacgctcga ctcttcaaa tccaaataa tggttatta ttaaacgat atattatac ttgacatgg ttgtaaata cgcttctct ccaattcaa tagatgttt gtctggtag ttgatgcac atattc
