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Gene information
General information
TC010596 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5221232..5251166
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010596
    iBeetle screen iB_04877

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature mostly thinner - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    longitudinal dorsal thoracic muscles posterior position irregular - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010596 RA
      MMSSEEETE FGLYTDKLL KKTKRTRQR VDAGEPRNS 
      YSSIPNFSS RPTFLGGGN NLYGAIFTQ QQQFGLFGP 
      GFAPAKMLN ELLGRQKAE GGESGEEGK FDAVIRCDG 
      SPPSGEQLA HHMLRDILQ GRKHELLAL EHDMRNGQD 
      HIINNNNNE PKAEPERND VEAAAAMKE CLAEAGVGD 
      RQQAELMEE ALNGMETES LPSPKVEPS SPAKPEDAK 
      KARVENIVS GMRSSPAPP QVNGCKKRK LYQPQQHDS 
      NAERYIDDE EDESAEPIR QKRVEKNLL KSQLRTMQE 
      QLAEMQQKY MQLCTRVEQ GSEESQEIE EIPSDSENS 
      KRSAPPSPI TTSNTPNQS TPSLAQQVS KLKISPPGP 
      PFHNGMLHH DHQHMSSAA AMYLGVSHK FLMEQEARF 
      AKEAAVAAM NSESSVQQS QHQSHHPSQ KPENIADRL 
      HIMRNMTPT GTDLEGLAD ALKTEITSS LSNLIDSIL 
      SRFVHQRRC KQTEAATAA EQLNKDLMM ASQLLDRKS 
      PRTKVVERP QAPPPPPNS QMVNGTSGC PPMMSVHMN 
      NMQKPNDLQ IRPPMFQPP KPPAISQAP LFGMSHPFC 
      VSKQENPEQ NEALSLVVT PKKKRHKVT DTRITPRTV 
      SRILGQDGI GMSPAAMEP NTCSSPRPP PYHQPPPML 
      PVSLPTSVA IPNPGLHES QVFSPYSPF FHGPSHGHH 
      MAMQASSSP PRMHKIERE SPPIHHPPT LLHPALLAA 
      AQHGGSPDY GQLRAPSGG MNMDNGDRN SDCNSGDIT 
      YDGMQPTIS FLCKDFSKN LSNHHTNIS LTPEHSSTL 
      TPMHLRKAK LMFFWVRYP SSAVLKMYF PDIKFNKNN 
      TAQLVKWFS NFREFYYIQ MEKYARQAV SEGVKNAED 
      LHVGGDSEL YRVLNLHYN RNNHIEVPS NFRFVVEQT 
      LREFFKAIQ EGRDAEQSW KKSIYKIIS RLDDPVPEY 
      FKSPNFLEQ LE
    CDS sequence
    • >TC010596 RA
      atgatgtca tcggaggag gagactgaa ttcggtttg 
      tacacagat aagctcctg aagaaaacc aaaagaact 
      agacagcgt gtagacgcc ggtgagccg cgtaactcc 
      tactcgtcg attccgaat ttcagttcg cgtccgact 
      tttctcggt ggcggtaac aatctgtac ggcgcaatt 
      ttcacccaa cagcagcag ttcggtctg ttcggaccc 
      gggttcgcg cccgcaaag atgctcaac gagctgctg 
      gggcggcag aaggccgaa ggcggcgaa agcggcgaa 
      gagggcaag ttcgacgcg gtgatccgc tgcgacggc 
      agcccgccc tcgggtgaa caactggcc catcacatg 
      ctccgggac atcctgcag ggccgcaag cacgagctt 
      ttggccttg gaacacgac atgcgcaac ggccaggac 
      cacatcatc aacaacaac aacaacgaa cccaaggcg 
      gaaccagaa cgcaacgac gtcgaagcc gccgccgcg 
      atgaaggag tgcctcgct gaggccgga gtcggcgac 
      cgccagcag gccgagctc atggaggag gcgctcaat 
      gggatggag accgagtcg ctgccctcg ccaaaggtg 
      gagccctcg agtccggcc aagccagag gatgcgaag 
      aaagcgcgc gtcgaaaac attgtctca ggtatgcgg 
      tcgagtccg gcgcctccg caagtcaac ggctgcaag 
      aaacgaaag ctctaccaa ccccagcaa cacgacagt 
      aacgctgaa cggtatatt gacgatgaa gaagatgaa 
      agtgcggaa ccaatcaga caaaaacgc gtcgagaaa 
      aatttactc aaatcgcag ctgagaacg atgcaagaa 
      cagttggcg gaaatgcag cagaaatac atgcagttg 
      tgcacgcgc gttgaacaa gggtctgaa gaaagtcaa 
      gaaattgaa gaaattccc agtgatagt gaaaacagt 
      aaacgatca gcgcctcct tcgcccata accaccagt 
      aatacccct aatcaatcg acgccaagc ttagcacaa 
      caggtgagc aaacttaaa ataagccct ccaggacct 
      cctttccac aatggtatg ttacatcat gatcaccag 
      cacatgagc agtgccgcc gccatgtac ttgggcgtg 
      agccacaaa ttcctgatg gagcaggaa gcccgtttt 
      gctaaagaa gcagcagtg gcagcaatg aacagcgaa 
      tcatcagtc caacaatcc cagcatcag tctcatcat 
      ccatcgcaa aaacccgaa aatatagcc gacagatta 
      cacattatg agaaacatg acaccgacc ggtaccgac 
      ttggaaggc ctcgccgac gctcttaaa accgaaatc 
      acgtcgtct ctttcaaat ttgatcgac tcgatacta 
      tcaaggttc gtccaccaa cggagatgc aaacagacg 
      gaggcggcg acagccgcc gaacaactc aacaaagac 
      ttgatgatg gcctcgcaa ttactcgac aggaaatcg 
      ccgcggacc aaagtagta gaacggcca caagcgccg 
      ccgccgccg cccaacagc cagatggtg aacggcacg 
      agcggctgc cctccgatg atgtccgtt cacatgaac 
      aacatgcaa aaacccaac gacctccaa ataaggcct 
      ccgatgttc cagcccccg aaacccccg gcaatcagc 
      caagcccct ctttttgga atgtcacac ccgttttgc 
      gtgtccaag caagaaaac cccgagcag aacgaagcg 
      ctcagcctc gtagtaact cctaagaaa aagcggcac 
      aaggtgacg gacacccgg ataacgccc aggaccgtg 
      agccgcatc ctgggccag gacggcatc ggcatgtcg 
      cccgccgcc atggagccc aacacgtgc agttccccg 
      cgcccgccc ccatatcac cagccgccg ccgatgttg 
      cccgtctcc cttccgacc tcggtggcg atccccaac 
      cccggactg cacgaatct caggtcttc tcgccctac 
      agccccttc tttcatgga ccaagtcac ggacaccac 
      atggcgatg caggcatcg tcatcacca cctcgcatg 
      cacaaaatc gagcgagag tcgcctcca atccaccac 
      cccccgacg cttctgcac cccgctctc ctggctgcg 
      gcccaacat gggggctcc cccgactac ggacagcta 
      agggcgccg tccgggggc atgaacatg gacaacggc 
      gatagaaat tctgattgt aactcaggg gacatcacc 
      tacgatgga atgcagcct actatatcc tttttatgc 
      aaagatttt agtaaaaat cttagtaac caccataca 
      aatatttcc ttaactcct gagcactcg tcaacactg 
      acaccgatg catctcagg aaagcgaaa ttaatgttc 
      ttctgggtt cggtatccg agctcagca gtactaaaa 
      atgtatttc cccgatatc aagtttaac aagaacaac 
      actgcccag ctcgtcaag tggttctcg aacttcagg 
      gagttctat tacatccag atggagaag tacgccaga 
      caggcagtt tccgaaggt gtgaagaac gctgaagat 
      ctgcatgtc ggtggtgat tccgaatta taccgggtg 
      ctcaatttg cactacaac agaaacaat cacatcgag 
      gttccttca aacttccgg ttcgtcgtg gagcagacg 
      ctccgggag tttttcaag gccatccag gagggccgg 
      gatgccgag cagtcatgg aagaagtcc atctacaag 
      atcatctcg cgactggac gacccggtc ccggagtac 
      ttcaagtcc cccaatttc ctggagcaa ttggagtga
    mRNA sequence
    • >TC010596 RA
      agtaaatgg aatcaaact tatcaatac caaccctcc 
      ctttcaaat aaaaggtat tgacatggg cgactattc 
      ttaaagtgg ggacgacta tctggaggg gggcacaga 
      acgaccaat aacgctgga acttatgat gtcatcgga 
      ggaggagac tgaattcgg tttgtacac agataagct 
      cctgaagaa aaccaaaag aactagaca gcgtgtaga 
      cgccggtga gccgcgtaa ctcctactc gtcgattcc 
      gaatttcag ttcgcgtcc gacttttct cggtggcgg 
      taacaatct gtacggcgc aattttcac ccaacagca 
      gcagttcgg tctgttcgg acccgggtt cgcgcccgc 
      aaagatgct caacgagct gctggggcg gcagaaggc 
      cgaaggcgg cgaaagcgg cgaagaggg caagttcga 
      cgcggtgat ccgctgcga cggcagccc gccctcggg 
      tgaacaact ggcccatca catgctccg ggacatcct 
      gcagggccg caagcacga gcttttggc cttggaaca 
      cgacatgcg caacggcca ggaccacat catcaacaa 
      caacaacaa cgaacccaa ggcggaacc agaacgcaa 
      cgacgtcga agccgccgc cgcgatgaa ggagtgcct 
      cgctgaggc cggagtcgg cgaccgcca gcaggccga 
      gctcatgga ggaggcgct caatgggat ggagaccga 
      gtcgctgcc ctcgccaaa ggtggagcc ctcgagtcc 
      ggccaagcc agaggatgc gaagaaagc gcgcgtcga 
      aaacattgt ctcaggtat gcggtcgag tccggcgcc 
      tccgcaagt caacggctg caagaaacg aaagctcta 
      ccaacccca gcaacacga cagtaacgc tgaacggta 
      tattgacga tgaagaaga tgaaagtgc ggaaccaat 
      cagacaaaa acgcgtcga gaaaaattt actcaaatc 
      gcagctgag aacgatgca agaacagtt ggcggaaat 
      gcagcagaa atacatgca gttgtgcac gcgcgttga 
      acaagggtc tgaagaaag tcaagaaat tgaagaaat 
      tcccagtga tagtgaaaa cagtaaacg atcagcgcc 
      tccttcgcc cataaccac cagtaatac ccctaatca 
      atcgacgcc aagcttagc acaacaggt gagcaaact 
      taaaataag ccctccagg acctccttt ccacaatgg 
      tatgttaca tcatgatca ccagcacat gagcagtgc 
      cgccgccat gtacttggg cgtgagcca caaattcct 
      gatggagca ggaagcccg ttttgctaa agaagcagc 
      agtggcagc aatgaacag cgaatcatc agtccaaca 
      atcccagca tcagtctca tcatccatc gcaaaaacc 
      cgaaaatat agccgacag attacacat tatgagaaa 
      catgacacc gaccggtac cgacttgga aggcctcgc 
      cgacgctct taaaaccga aatcacgtc gtctctttc 
      aaatttgat cgactcgat actatcaag gttcgtcca 
      ccaacggag atgcaaaca gacggaggc ggcgacagc 
      cgccgaaca actcaacaa agacttgat gatggcctc 
      gcaattact cgacaggaa atcgccgcg gaccaaagt 
      agtagaacg gccacaagc gccgccgcc gccgcccaa 
      cagccagat ggtgaacgg cacgagcgg ctgccctcc 
      gatgatgtc cgttcacat gaacaacat gcaaaaacc 
      caacgacct ccaaataag gcctccgat gttccagcc 
      cccgaaacc cccggcaat cagccaagc ccctctttt 
      tggaatgtc acacccgtt ttgcgtgtc caagcaaga 
      aaaccccga gcagaacga agcgctcag cctcgtagt 
      aactcctaa gaaaaagcg gcacaaggt gacggacac 
      ccggataac gcccaggac cgtgagccg catcctggg 
      ccaggacgg catcggcat gtcgcccgc cgccatgga 
      gcccaacac gtgcagttc cccgcgccc gcccccata 
      tcaccagcc gccgccgat gttgcccgt ctcccttcc 
      gacctcggt ggcgatccc caaccccgg actgcacga 
      atctcaggt cttctcgcc ctacagccc cttctttca 
      tggaccaag tcacggaca ccacatggc gatgcaggc 
      atcgtcatc accacctcg catgcacaa aatcgagcg 
      agagtcgcc tccaatcca ccacccccc gacgcttct 
      gcaccccgc tctcctggc tgcggccca acatggggg 
      ctcccccga ctacggaca gctaagggc gccgtccgg 
      gggcatgaa catggacaa cggcgatag aaattctga 
      ttgtaactc aggggacat cacctacga tggaatgca 
      gcctactat atccttttt atgcaaaga ttttagtaa 
      aaatcttag taaccacca tacaaatat ttccttaac 
      tcctgagca ctcgtcaac actgacacc gatgcatct 
      caggaaagc gaaattaat gttcttctg ggttcggta 
      tccgagctc agcagtact aaaaatgta tttccccga 
      tatcaagtt taacaagaa caacactgc ccagctcgt 
      caagtggtt ctcgaactt cagggagtt ctattacat 
      ccagatgga gaagtacgc cagacaggc agtttccga 
      aggtgtgaa gaacgctga agatctgca tgtcggtgg 
      tgattccga attataccg ggtgctcaa tttgcacta 
      caacagaaa caatcacat cgaggttcc ttcaaactt 
      ccggttcgt cgtggagca gacgctccg ggagttttt 
      caaggccat ccaggaggg ccgggatgc cgagcagtc 
      atggaagaa gtccatcta caagatcat ctcgcgact 
      ggacgaccc ggtcccgga gtacttcaa gtcccccaa 
      tttcctgga gcaattgga gtgaccggt tggccggac 
      cgagaagcc ggtatccag cggtagcca gtggctacc 
      gtgcggtac catccgtta gacacggca aaaacacca 
      gagagaatc ggtgacacg tgacaaaac tgtcaacag 
      tttatatgt tgttttgcc gggaatgag cttggagga 
      cgccgcttt gacgccatc ttcgaaact tgttccgga 
      cactcttgt taaatagtt gtgtttagt tgacagaaa 
      gacaatttt atcgcctca ggctttatg ttcgttcaa 
      acgaacagt gaacacaaa tcaatcaat tttccagtg 
      attacctga atatttacc ggatagaat tttatgacg 
      tcaagtctt tcttttctt ttcaaccag cagtcatca 
      agttcaaaa tctgcactc tcattgcgt tacttctgg 
      cattattat gttcggtgc cactgtaaa ctgcccctt 
      ttacccctg tatataaag cgacgtgta catagtatt 
      tatcactta attttattt atatcttgt attattatt 
      atcatctca accaaagtt tttatttta ctagtccca 
      cataagatt attcccttt tgaataatt ttacgtaaa 
      ctatcagtg ccaaacttt gaacacaat acaacgctt 
      caaagtttc gcactgaaa aaagattta ttgcagcaa 
      ttattcttc cattttgta tcaaataca actcgaaag 
      tctaattcc tgtgattta ttcggtatt aagtaggta 
      tgccttgtt ttgtattta ttattttgt accccaaca 
      ctcccccat tctgtttta atttattag ttgtagact 
      agttgctat tattatttc tgttagcat acctaccta 
      attacctgt tttatttta tattttagt taattttaa 
      tcacttgca caatcaaat agtgtaggt tctacacga 
      tggcaaata cagtgtgag gcaactagt cagtaaaaa 
      taaaatgta ttatcttta tttggtttt attttggtt 
      tgtttcttt ttggttcga agacagttt acagtgtga 
      tgtttcgag tacaataat tttgagcca taaatcgta 
      aattgtaca taaatttgg agctcatgg tttccttca 
      catcccgca atagttgag gtcacatac acaatcgaa 
      ggccccgag agcccttaa cttagcctt atggtgagg 
      ctaggaggt cgtgatttc gctgttcct aaaatccta 
      tattcgaga tactgccga aattgtaag actgccatt 
      cacttaaga ttgatttag cggtttcgt tgtcgtttc 
      gcgtttatt tgcgtttca aaattaatc gtttggatg 
      tttgtgacg ttgcgttga tttttcggt ttgttctcg 
      gggcctgca tcggtacat tacataaga aaaataaaa 
      acaaaattt aaaaattag attaatcta aatagttat 
      ttattaaga tttaaagta tataacgtt gtcgactgt 
      aaagtaccc gtaatgctg atggttttc aaacaagca 
      aacgctcga ctcttcaaa tccaaataa tggttatta 
      ttaaacgat atattatac ttgacatgg ttgtaaata 
      cgcttctct ccaattcaa tagatgttt gtctggtag 
      ttgatgcac atattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY