Status
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Gene information
General information
TC010590 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5383965..5387712
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010590
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04875

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010590 RA
      MALVRSCFC CSLAIASGF FAVYLLIAY LIAFAFELL 
      WILESLVAL PAAAVLLCA GYFTMALFA ALLIHGLAT 
      KNTLCLIAW MFAVTILTF PEAGLVIYM SIQYWKIET 
      LYGVTELAS WLVRIVVNV IQLILIQSL YTTWKDEEI 
      VEKRLRDFT MAPIPIPRE SLASLNSQF YQNNAYDNS 
      EEMAMGLKR AASTPHIWS NAVPNNLAA VQFDGFQFC 
      TTQSEFNAS VFVPSYINS DSLSKKAQS LMDLRILED 
      GQDKVNFPS MVSKTITET SSAIIPNFE VSPPRMHKY 
      ASVDALNDF NKSSHVIKN RTGFYAQPI PNYGVPIYY 
      GPLDGPDFL IYKKQVDKL SSKNSLSNA SADDVQKYR 
      DVAL
    CDS sequence
    • >TC010590 RA
      atggctcta gtgcgatcg tgtttctgc tgcagctta 
      gcaatagcg tccggattt tttgcagtt tatcttctg 
      atcgcgtac ctgatagcc tttgctttt gaattgcta 
      tggattctg gagtccttg gtggccttg cctgccgca 
      gcagtcttg ttgtgtgcg ggatatttc acaatggcc 
      ctgtttgca gcattgcta atccacgga ctagcgacg 
      aaaaatact ctttgtctc atcgcctgg atgttcgct 
      gtgaccata ttaacgttc ccagaggcg ggtttagtc 
      atttacatg agcatccag tactggaag atcgaaact 
      ttatacgga gtgacagaa ctggcgagc tggttggtc 
      cgcattgta gtaaatgtg atacagtta attttaatc 
      cagtctctc tacacgaca tggaaagac gaagaaata 
      gtcgagaag cgactgagg gacttcacc atggccccc 
      attcccata ccgcgcgag tccctggcg tccctcaac 
      tcgcagttt taccaaaat aacgcctac gacaattcc 
      gaggagatg gcaatgggg ctcaaacgg gccgcctcc 
      acgccccac atctggtcg aacgcagtg cccaacaac 
      ctggcggcg gtccaattc gacgggttt caattttgc 
      acgacccag agcgagttt aatgcgagc gttttcgtg 
      ccaagttac atcaacagc gacagtttg agtaagaaa 
      gcccagtcc ttgatggat ttgaggatt ctcgaggat 
      ggccaggac aaagtgaac ttcccgtcg atggtgtcc 
      aagaccatc acggagaca agtagcgcc attatcccg 
      aatttcgaa gtgagcccc cccaggatg cacaagtac 
      gcctctgtg gacgccctc aacgacttc aacaagtcc 
      agtcatgtg atcaagaac cggaccggc ttttacgcg 
      cagcctatc cccaattac ggggttccc atttattac 
      ggccctctg gacgggcct gattttctc atttacaaa 
      aaacaagtc gataagttg agtagtaag aactcgttg 
      agtaacgcg tccgctgac gacgtgcag aagtacaga 
      gacgttgct ttgtaa
    mRNA sequence
    • >TC010590 RA
      aaccactcc agtctcgaa ggttaaacg tcttaatac 
      gaaagagaa acaacaatg gttttgaaa atcatttaa 
      acatacatt tatgtgtta cataaaaga aaattattt 
      tgaacgtga aagtgtaaa cttatcgga aaaaaatta 
      cgtgggatt accaaaaca gtgaaagtg cgaaaatag 
      tgcgatttc aaaaatgtg attccaatt gtgaaaatt 
      aaaatgtag tgcaaatta gtgaaaatg gctctagtg 
      cgatcgtgt ttctgctgc agcttagca atagcgtcc 
      ggatttttt gcagtttat cttctgatc gcgtacctg 
      atagccttt gcttttgaa ttgctatgg attctggag 
      tccttggtg gccttgcct gccgcagca gtcttgttg 
      tgtgcggga tatttcaca atggccctg tttgcagca 
      ttgctaatc cacggacta gcgacgaaa aatactctt 
      tgtctcatc gcctggatg ttcgctgtg accatatta 
      acgttccca gaggcgggt ttagtcatt tacatgagc 
      atccagtac tggaagatc gaaacttta tacggagtg 
      acagaactg gcgagctgg ttggtccgc attgtagta 
      aatgtgata cagttaatt ttaatccag tctctctac 
      acgacatgg aaagacgaa gaaatagtc gagaagcga 
      ctgagggac ttcaccatg gcccccatt cccataccg 
      cgcgagtcc ctggcgtcc ctcaactcg cagttttac 
      caaaataac gcctacgac aattccgag gagatggca 
      atggggctc aaacgggcc gcctccacg ccccacatc 
      tggtcgaac gcagtgccc aacaacctg gcggcggtc 
      caattcgac gggtttcaa ttttgcacg acccagagc 
      gagtttaat gcgagcgtt ttcgtgcca agttacatc 
      aacagcgac agtttgagt aagaaagcc cagtccttg 
      atggatttg aggattctc gaggatggc caggacaaa 
      gtgaacttc ccgtcgatg gtgtccaag accatcacg 
      gagacaagt agcgccatt atcccgaat ttcgaagtg 
      agccccccc aggatgcac aagtacgcc tctgtggac 
      gccctcaac gacttcaac aagtccagt catgtgatc 
      aagaaccgg accggcttt tacgcgcag cctatcccc 
      aattacggg gttcccatt tattacggc cctctggac 
      gggcctgat tttctcatt tacaaaaaa caagtcgat 
      aagttgagt agtaagaac tcgttgagt aacgcgtcc 
      gctgacgac gtgcagaag tacagagac gttgctttg 
      taaaaggtg ccatttaag tatttacgt aaacggttg 
      tgtattagg caata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY