| General information | |||
| TC010578 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 5137198..5142735 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010578 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04874 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010578 RA
MLEARAMKS QIAEFFANQ TVFLTGGGG FLGKVLIEK LLRECSDVS KIYMLMRQK KGKTPQQRV SEVFDFPCF DYIKANNAA IIKKVHVIH GDCQKPDLG LDPEDVRVL KAETTCVIH AAASVRFDQ SLKEASYNV RATRDILEL AKQMANLKC FIFVSTAYS NPLNAHVKE DFYEPPIEA EKLLNVVNS LDDSVLQRV TPQLLGEWP NTYVYTKSI SESLIRSID TFPIAIIRP GIILSSAKE PMPGWIDNF YGPVGIATG AGVGVLKTF HAKRDAVAA MVPVDYVVN ALLSIMWRI GTEKTKTPT VYNFAGHKT NKITWGQFT DNLRELYWI AIPKCLFWY VSFVLRENQ ISYKLTTFF SHTLFGYII DFVLFCLGK RTLAVKGYA RLHKTLDLI AWFSTRTWD FDDNNILKL WSEMGEEDK KIFDFNMEK LNWDAYLRD SAFGMRYFL LKESLETVP EAKRKLRIM FVAHYAIII LFWFLMYKL MTFMYGLFV
- >TC010578 RA
atgctagaa gctagagcc atgaagagc caaattgct gaatttttc gcaaaccaa acggttttt ctaactggg ggaggaggc tttttgggt aaagtcttg atcgaaaaa ctcctacga gaatgttcc gatgtgtcg aaaatttat atgctgatg cggcaaaag aagggaaaa acaccccag cagcgtgtg agcgaagtg ttcgacttt ccttgtttt gattacatc aaagccaac aatgcggct atcattaag aaagtgcac gtcattcat ggtgactgt cagaaaccg gatttggga ttggaccct gaagatgtg agggtgtta aaagccgaa actacgtgc gttattcac gctgcagca agtgttagg tttgaccaa tcgctgaaa gaggctagt tacaatgtt cgtgccaca agagatatc ctggaattg gctaaacaa atggcaaat ttgaaatgt ttcattttc gtttccacg gcttattcc aaccctctt aatgctcac gtgaaagaa gatttttac gagccacca atcgaagcc gaaaagcta ctaaatgtt gtgaattcc ctcgacgat agtgtgctt caaagagtc acaccacaa cttttggga gaatggccc aatacttac gtttacacc aaatcaatt tccgaaagt ttgatacga tctattgat acttttccc attgccatc atacgtcca ggaattatt cttagttcg gctaaggaa ccaatgcca ggctggatt gataatttc tatggtcct gttggtata gccacgggt gcgggtgtg ggagttttg aaaactttc cacgcaaaa cgcgatgct gtggccgcc atggtacca gttgattat gtcgttaat gcccttttg tcaattatg tggagaata ggtaccgaa aaaacgaaa actccaaca gtgtacaat tttgctggg cacaaaacc aacaaaatc acttgggga cagtttacg gataattta agagagctt tattggata gctatccct aaatgtctt ttttggtac gtctcgttt gtactgaga gaaaaccaa atttcctac aaactgaca acatttttt tcgcatacc ctctttggt tacatcatt gattttgtc ctcttctgt ttagggaaa cgcactctg gcggttaaa gggtatgct cgactgcat aaaaccctt gatttaatc gcatggttt tcaacaagg acatgggac ttcgatgac aataatatc ttaaaacta tggagcgaa atgggggaa gaagataaa aaaatattt gatttcaat atggaaaaa ctaaactgg gacgcttac ctacgcgac tctgctttt gggatgaga tattttttg ctgaaggaa tcattagag acagttcct gaagccaaa agaaaactc agaattatg tttgttgcg cattacgcc attattata ttattttgg ttcttgatg tacaagctt atgacattt atgtatgga ttgtttgtc taa
- >TC010578 RA
gatacaccc agacccaag tagtaaatg cggacccgt cctagaaat gcattaacg attcgcgag cattagtct gcgagttct tcgcagttg cggcgaata tttcatcgg caaagattt aatactaga ttaatagca aatgcccct taaaaattc accagtcga cgccactga ataacattt gcaaattac cattcaatt ttgacaatg ctagaagct agagccatg aagagccaa attgctgaa tttttcgca aaccaaacg gtttttcta actggggga ggaggcttt ttgggtaaa gtcttgatc gaaaaactc ctacgagaa tgttccgat gtgtcgaaa atttatatg ctgatgcgg caaaagaag ggaaaaaca ccccagcag cgtgtgagc gaagtgttc gactttcct tgttttgat tacatcaaa gccaacaat gcggctatc attaagaaa gtgcacgtc attcatggt gactgtcag aaaccggat ttgggattg gaccctgaa gatgtgagg gtgttaaaa gccgaaact acgtgcgtt attcacgct gcagcaagt gttaggttt gaccaatcg ctgaaagag gctagttac aatgttcgt gccacaaga gatatcctg gaattggct aaacaaatg gcaaatttg aaatgtttc attttcgtt tccacggct tattccaac cctcttaat gctcacgtg aaagaagat ttttacgag ccaccaatc gaagccgaa aagctacta aatgttgtg aattccctc gacgatagt gtgcttcaa agagtcaca ccacaactt ttgggagaa tggcccaat acttacgtt tacaccaaa tcaatttcc gaaagtttg atacgatct attgatact tttcccatt gccatcata cgtccagga attattctt agttcggct aaggaacca atgccaggc tggattgat aatttctat ggtcctgtt ggtatagcc acgggtgcg ggtgtggga gttttgaaa actttccac gcaaaacgc gatgctgtg gccgccatg gtaccagtt gattatgtc gttaatgcc cttttgtca attatgtgg agaataggt accgaaaaa acgaaaact ccaacagtg tacaatttt gctgggcac aaaaccaac aaaatcact tggggacag tttacggat aatttaaga gagctttat tggatagct atccctaaa tgtcttttt tggtacgtc tcgtttgta ctgagagaa aaccaaatt tcctacaaa ctgacaaca tttttttcg cataccctc tttggttac atcattgat tttgtcctc ttctgttta gggaaacgc actctggcg gttaaaggg tatgctcga ctgcataaa acccttgat ttaatcgca tggttttca acaaggaca tgggacttc gatgacaat aatatctta aaactatgg agcgaaatg ggggaagaa gataaaaaa atatttgat ttcaatatg gaaaaacta aactgggac gcttaccta cgcgactct gcttttggg atgagatat tttttgctg aaggaatca ttagagaca gttcctgaa gccaaaaga aaactcaga attatgttt gttgcgcat tacgccatt attatatta ttttggttc ttgatgtac aagcttatg acatttatg tatggattg tttgtctaa ctaaaaata taaaaaaaa ctgtacc
