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Gene information
General information
TC010578 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5137198..5142735
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010578
    iBeetle screen iB_04874

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010578 RA
      MLEARAMKS QIAEFFANQ TVFLTGGGG FLGKVLIEK 
      LLRECSDVS KIYMLMRQK KGKTPQQRV SEVFDFPCF 
      DYIKANNAA IIKKVHVIH GDCQKPDLG LDPEDVRVL 
      KAETTCVIH AAASVRFDQ SLKEASYNV RATRDILEL 
      AKQMANLKC FIFVSTAYS NPLNAHVKE DFYEPPIEA 
      EKLLNVVNS LDDSVLQRV TPQLLGEWP NTYVYTKSI 
      SESLIRSID TFPIAIIRP GIILSSAKE PMPGWIDNF 
      YGPVGIATG AGVGVLKTF HAKRDAVAA MVPVDYVVN 
      ALLSIMWRI GTEKTKTPT VYNFAGHKT NKITWGQFT 
      DNLRELYWI AIPKCLFWY VSFVLRENQ ISYKLTTFF 
      SHTLFGYII DFVLFCLGK RTLAVKGYA RLHKTLDLI 
      AWFSTRTWD FDDNNILKL WSEMGEEDK KIFDFNMEK 
      LNWDAYLRD SAFGMRYFL LKESLETVP EAKRKLRIM 
      FVAHYAIII LFWFLMYKL MTFMYGLFV
    CDS sequence
    • >TC010578 RA
      atgctagaa gctagagcc atgaagagc caaattgct 
      gaatttttc gcaaaccaa acggttttt ctaactggg 
      ggaggaggc tttttgggt aaagtcttg atcgaaaaa 
      ctcctacga gaatgttcc gatgtgtcg aaaatttat 
      atgctgatg cggcaaaag aagggaaaa acaccccag 
      cagcgtgtg agcgaagtg ttcgacttt ccttgtttt 
      gattacatc aaagccaac aatgcggct atcattaag 
      aaagtgcac gtcattcat ggtgactgt cagaaaccg 
      gatttggga ttggaccct gaagatgtg agggtgtta 
      aaagccgaa actacgtgc gttattcac gctgcagca 
      agtgttagg tttgaccaa tcgctgaaa gaggctagt 
      tacaatgtt cgtgccaca agagatatc ctggaattg 
      gctaaacaa atggcaaat ttgaaatgt ttcattttc 
      gtttccacg gcttattcc aaccctctt aatgctcac 
      gtgaaagaa gatttttac gagccacca atcgaagcc 
      gaaaagcta ctaaatgtt gtgaattcc ctcgacgat 
      agtgtgctt caaagagtc acaccacaa cttttggga 
      gaatggccc aatacttac gtttacacc aaatcaatt 
      tccgaaagt ttgatacga tctattgat acttttccc 
      attgccatc atacgtcca ggaattatt cttagttcg 
      gctaaggaa ccaatgcca ggctggatt gataatttc 
      tatggtcct gttggtata gccacgggt gcgggtgtg 
      ggagttttg aaaactttc cacgcaaaa cgcgatgct 
      gtggccgcc atggtacca gttgattat gtcgttaat 
      gcccttttg tcaattatg tggagaata ggtaccgaa 
      aaaacgaaa actccaaca gtgtacaat tttgctggg 
      cacaaaacc aacaaaatc acttgggga cagtttacg 
      gataattta agagagctt tattggata gctatccct 
      aaatgtctt ttttggtac gtctcgttt gtactgaga 
      gaaaaccaa atttcctac aaactgaca acatttttt 
      tcgcatacc ctctttggt tacatcatt gattttgtc 
      ctcttctgt ttagggaaa cgcactctg gcggttaaa 
      gggtatgct cgactgcat aaaaccctt gatttaatc 
      gcatggttt tcaacaagg acatgggac ttcgatgac 
      aataatatc ttaaaacta tggagcgaa atgggggaa 
      gaagataaa aaaatattt gatttcaat atggaaaaa 
      ctaaactgg gacgcttac ctacgcgac tctgctttt 
      gggatgaga tattttttg ctgaaggaa tcattagag 
      acagttcct gaagccaaa agaaaactc agaattatg 
      tttgttgcg cattacgcc attattata ttattttgg 
      ttcttgatg tacaagctt atgacattt atgtatgga 
      ttgtttgtc taa
    mRNA sequence
    • >TC010578 RA
      gatacaccc agacccaag tagtaaatg cggacccgt 
      cctagaaat gcattaacg attcgcgag cattagtct 
      gcgagttct tcgcagttg cggcgaata tttcatcgg 
      caaagattt aatactaga ttaatagca aatgcccct 
      taaaaattc accagtcga cgccactga ataacattt 
      gcaaattac cattcaatt ttgacaatg ctagaagct 
      agagccatg aagagccaa attgctgaa tttttcgca 
      aaccaaacg gtttttcta actggggga ggaggcttt 
      ttgggtaaa gtcttgatc gaaaaactc ctacgagaa 
      tgttccgat gtgtcgaaa atttatatg ctgatgcgg 
      caaaagaag ggaaaaaca ccccagcag cgtgtgagc 
      gaagtgttc gactttcct tgttttgat tacatcaaa 
      gccaacaat gcggctatc attaagaaa gtgcacgtc 
      attcatggt gactgtcag aaaccggat ttgggattg 
      gaccctgaa gatgtgagg gtgttaaaa gccgaaact 
      acgtgcgtt attcacgct gcagcaagt gttaggttt 
      gaccaatcg ctgaaagag gctagttac aatgttcgt 
      gccacaaga gatatcctg gaattggct aaacaaatg 
      gcaaatttg aaatgtttc attttcgtt tccacggct 
      tattccaac cctcttaat gctcacgtg aaagaagat 
      ttttacgag ccaccaatc gaagccgaa aagctacta 
      aatgttgtg aattccctc gacgatagt gtgcttcaa 
      agagtcaca ccacaactt ttgggagaa tggcccaat 
      acttacgtt tacaccaaa tcaatttcc gaaagtttg 
      atacgatct attgatact tttcccatt gccatcata 
      cgtccagga attattctt agttcggct aaggaacca 
      atgccaggc tggattgat aatttctat ggtcctgtt 
      ggtatagcc acgggtgcg ggtgtggga gttttgaaa 
      actttccac gcaaaacgc gatgctgtg gccgccatg 
      gtaccagtt gattatgtc gttaatgcc cttttgtca 
      attatgtgg agaataggt accgaaaaa acgaaaact 
      ccaacagtg tacaatttt gctgggcac aaaaccaac 
      aaaatcact tggggacag tttacggat aatttaaga 
      gagctttat tggatagct atccctaaa tgtcttttt 
      tggtacgtc tcgtttgta ctgagagaa aaccaaatt 
      tcctacaaa ctgacaaca tttttttcg cataccctc 
      tttggttac atcattgat tttgtcctc ttctgttta 
      gggaaacgc actctggcg gttaaaggg tatgctcga 
      ctgcataaa acccttgat ttaatcgca tggttttca 
      acaaggaca tgggacttc gatgacaat aatatctta 
      aaactatgg agcgaaatg ggggaagaa gataaaaaa 
      atatttgat ttcaatatg gaaaaacta aactgggac 
      gcttaccta cgcgactct gcttttggg atgagatat 
      tttttgctg aaggaatca ttagagaca gttcctgaa 
      gccaaaaga aaactcaga attatgttt gttgcgcat 
      tacgccatt attatatta ttttggttc ttgatgtac 
      aagcttatg acatttatg tatggattg tttgtctaa 
      ctaaaaata taaaaaaaa ctgtacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY