Status
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Gene information
General information
TC010570 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 137178..138574
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010570
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04872

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010570 RA
      MNDIITIPV VLSSEMLFG IPVTYSSDE DSDVTDEEN 
      IVTMMLIKS RKIKKPQRI ENYIEATIS GYTKQEFQQ 
      HFRVTIEAY EQLLQTVGP YLMRQAATG RSTVQVEKQ 
      LLSVIWILV TPDSYRSVG ERFGLAKSS LSQCFKRVV 
      KILNAVAPT IIKWPEGMQ LQNVERRFH AFARMPHCI 
      GAIDGTYVE IKAPKEDPQ SYITRKCNY AFTLQAIAV 
      PSLKFTDAF IGYPGSVSD SRIFKNSDF YNAVNANMG 
      HYFAEEHYL IGDKAYPNL PWCIAPYIN RGNLNAAQV 
      YFNTVHAQT RQVIERSFA LLFGRFRRL KFLDMNDTK 
      LIPATVIAA CVLHNVCLD FHDLHIDDY INNGIDYVV 
      NNNDGDDGI GENVAIEGR RRRDALCRQ IFLNRH
    CDS sequence
    • >TC010570 RA
      atgaatgac ataattact atccctgtt gtattatca 
      tcggaaatg ttatttggc attccggtt acatattct 
      tctgatgag gacagcgat gtaactgat gaagaaaat 
      attgttacg atgatgctt ataaaaagt aggaaaatt 
      aagaaacct caacgtatt gagaattat attgaagca 
      acaatttct ggttatacc aagcaagaa tttcagcaa 
      cattttcga gtaaccatt gaggcttat gaacaactc 
      ttacaaaca gttggcccc tatttaatg cgacaagct 
      gctactgga cgaagtact gttcaagtc gaaaaacaa 
      ttgttgtca gttatatgg atactcgta acacccgat 
      tcatacaga tcagttgga gaaagattt ggactagca 
      aagtcatct ctttcacag tgtttcaaa cgagtagta 
      aaaattcta aatgccgta gcaccaact attataaaa 
      tggccagaa gggatgcaa ttacaaaat gttgagaga 
      agatttcat gcatttgcc agaatgcca cactgcatt 
      ggggccatt gatggtaca tacgtggaa atcaaagct 
      cctaaagag gatcctcaa tcctatatt acacgaaaa 
      tgcaactat gcttttacg ctgcaagct atcgcagta 
      ccttcattg aaatttaca gatgcattc attggttat 
      ccaggatct gtaagtgat agcagaata tttaaaaac 
      agtgatttt tataatgct gtcaatgcc aacatgggt 
      cattacttt gccgaagaa cattattta attggagat 
      aaagcatac cctaattta ccatggtgt attgcacca 
      tacataaat aggggtaac ctgaatgca gcacaggta 
      tacttcaac actgtacat gcacagacg cgccaagta 
      atcgaacga tcgtttgct cttctattt ggaagattt 
      aggcgactc aagttttta gacatgaat gatactaaa 
      ttaattcct gcaactgtg atagctgca tgcgtatta 
      cacaatgtc tgtctagac tttcatgat ctccatatt 
      gatgactat attaacaat ggaattgat tatgtagta 
      aataataat gatggtgac gatggcatt ggtgaaaac 
      gtggcaata gaaggacgg agacgacgt gatgcttta 
      tgtcgtcaa atatttctt aatcgacat taa
    mRNA sequence
    • >TC010570 RA
      ccgtaacaa tgaatgaca taattacta tccctgttg 
      tattatcat cggaaatgt tatttggca ttccggtta 
      catattctt ctgatgagg acagcgatg taactgatg 
      aagaaaata ttgttacga tgatgctta taaaaagta 
      ggaaaatta agaaacctc aacgtattg agaattata 
      ttgaagcaa caatttctg gttatacca agcaagaat 
      ttcagcaac attttcgag taaccattg aggcttatg 
      aacaactct tacaaacag ttggcccct atttaatgc 
      gacaagctg ctactggac gaagtactg ttcaagtcg 
      aaaaacaat tgttgtcag ttatatgga tactcgtaa 
      cacccgatt catacagat cagttggag aaagatttg 
      gactagcaa agtcatctc tttcacagt gtttcaaac 
      gagtagtaa aaattctaa atgccgtag caccaacta 
      ttataaaat ggccagaag ggatgcaat tacaaaatg 
      ttgagagaa gatttcatg catttgcca gaatgccac 
      actgcattg gggccattg atggtacat acgtggaaa 
      tcaaagctc ctaaagagg atcctcaat cctatatta 
      cacgaaaat gcaactatg cttttacgc tgcaagcta 
      tcgcagtac cttcattga aatttacag atgcattca 
      ttggttatc caggatctg taagtgata gcagaatat 
      ttaaaaaca gtgattttt ataatgctg tcaatgcca 
      acatgggtc attactttg ccgaagaac attatttaa 
      ttggagata aagcatacc ctaatttac catggtgta 
      ttgcaccat acataaata ggggtaacc tgaatgcag 
      cacaggtat acttcaaca ctgtacatg cacagacgc 
      gccaagtaa tcgaacgat cgtttgctc ttctatttg 
      gaagattta ggcgactca agtttttag acatgaatg 
      atactaaat taattcctg caactgtga tagctgcat 
      gcgtattac acaatgtct gtctagact ttcatgatc 
      tccatattg atgactata ttaacaatg gaattgatt 
      atgtagtaa ataataatg atggtgacg atggcattg 
      gtgaaaacg tggcaatag aaggacgga gacgacgtg 
      atgctttat gtcgtcaaa tatttctta atcgacatt 
      aattgtacg cttttcttg taagttttt gttaattgg 
      tatatttaa gttacttat agggttata cctacctct 
      attgtaaag ttatcgtaa acttcatct ttaacgtgt 
      tccaaattg ctggtattt ttttgtact tcttaaaaa 
      tatatgtta tttaagaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY