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Gene information
General information
TC010570 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 137178..138574
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010570
    iBeetle screen iB_04872

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010570 RA
      MNDIITIPV VLSSEMLFG IPVTYSSDE DSDVTDEEN 
IVTMMLIKS RKIKKPQRI ENYIEATIS GYTKQEFQQ 
HFRVTIEAY EQLLQTVGP YLMRQAATG RSTVQVEKQ 
LLSVIWILV TPDSYRSVG ERFGLAKSS LSQCFKRVV 
KILNAVAPT IIKWPEGMQ LQNVERRFH AFARMPHCI 
GAIDGTYVE IKAPKEDPQ SYITRKCNY AFTLQAIAV 
PSLKFTDAF IGYPGSVSD SRIFKNSDF YNAVNANMG 
HYFAEEHYL IGDKAYPNL PWCIAPYIN RGNLNAAQV 
YFNTVHAQT RQVIERSFA LLFGRFRRL KFLDMNDTK 
LIPATVIAA CVLHNVCLD FHDLHIDDY INNGIDYVV 
NNNDGDDGI GENVAIEGR RRRDALCRQ IFLNRH
    CDS sequence
    • >TC010570 RA
      atgaatgac ataattact atccctgtt gtattatca 
tcggaaatg ttatttggc attccggtt acatattct 
tctgatgag gacagcgat gtaactgat gaagaaaat 
attgttacg atgatgctt ataaaaagt aggaaaatt 
aagaaacct caacgtatt gagaattat attgaagca 
acaatttct ggttatacc aagcaagaa tttcagcaa 
cattttcga gtaaccatt gaggcttat gaacaactc 
ttacaaaca gttggcccc tatttaatg cgacaagct 
gctactgga cgaagtact gttcaagtc gaaaaacaa 
ttgttgtca gttatatgg atactcgta acacccgat 
tcatacaga tcagttgga gaaagattt ggactagca 
aagtcatct ctttcacag tgtttcaaa cgagtagta 
aaaattcta aatgccgta gcaccaact attataaaa 
tggccagaa gggatgcaa ttacaaaat gttgagaga 
agatttcat gcatttgcc agaatgcca cactgcatt 
ggggccatt gatggtaca tacgtggaa atcaaagct 
cctaaagag gatcctcaa tcctatatt acacgaaaa 
tgcaactat gcttttacg ctgcaagct atcgcagta 
ccttcattg aaatttaca gatgcattc attggttat 
ccaggatct gtaagtgat agcagaata tttaaaaac 
agtgatttt tataatgct gtcaatgcc aacatgggt 
cattacttt gccgaagaa cattattta attggagat 
aaagcatac cctaattta ccatggtgt attgcacca 
tacataaat aggggtaac ctgaatgca gcacaggta 
tacttcaac actgtacat gcacagacg cgccaagta 
atcgaacga tcgtttgct cttctattt ggaagattt 
aggcgactc aagttttta gacatgaat gatactaaa 
ttaattcct gcaactgtg atagctgca tgcgtatta 
cacaatgtc tgtctagac tttcatgat ctccatatt 
gatgactat attaacaat ggaattgat tatgtagta 
aataataat gatggtgac gatggcatt ggtgaaaac 
gtggcaata gaaggacgg agacgacgt gatgcttta 
tgtcgtcaa atatttctt aatcgacat taa
    mRNA sequence
    • >TC010570 RA
      ccgtaacaa tgaatgaca taattacta tccctgttg 
tattatcat cggaaatgt tatttggca ttccggtta 
catattctt ctgatgagg acagcgatg taactgatg 
aagaaaata ttgttacga tgatgctta taaaaagta 
ggaaaatta agaaacctc aacgtattg agaattata 
ttgaagcaa caatttctg gttatacca agcaagaat 
ttcagcaac attttcgag taaccattg aggcttatg 
aacaactct tacaaacag ttggcccct atttaatgc 
gacaagctg ctactggac gaagtactg ttcaagtcg 
aaaaacaat tgttgtcag ttatatgga tactcgtaa 
cacccgatt catacagat cagttggag aaagatttg 
gactagcaa agtcatctc tttcacagt gtttcaaac 
gagtagtaa aaattctaa atgccgtag caccaacta 
ttataaaat ggccagaag ggatgcaat tacaaaatg 
ttgagagaa gatttcatg catttgcca gaatgccac 
actgcattg gggccattg atggtacat acgtggaaa 
tcaaagctc ctaaagagg atcctcaat cctatatta 
cacgaaaat gcaactatg cttttacgc tgcaagcta 
tcgcagtac cttcattga aatttacag atgcattca 
ttggttatc caggatctg taagtgata gcagaatat 
ttaaaaaca gtgattttt ataatgctg tcaatgcca 
acatgggtc attactttg ccgaagaac attatttaa 
ttggagata aagcatacc ctaatttac catggtgta 
ttgcaccat acataaata ggggtaacc tgaatgcag 
cacaggtat acttcaaca ctgtacatg cacagacgc 
gccaagtaa tcgaacgat cgtttgctc ttctatttg 
gaagattta ggcgactca agtttttag acatgaatg 
atactaaat taattcctg caactgtga tagctgcat 
gcgtattac acaatgtct gtctagact ttcatgatc 
tccatattg atgactata ttaacaatg gaattgatt 
atgtagtaa ataataatg atggtgacg atggcattg 
gtgaaaacg tggcaatag aaggacgga gacgacgtg 
atgctttat gtcgtcaaa tatttctta atcgacatt 
aattgtacg cttttcttg taagttttt gttaattgg 
tatatttaa gttacttat agggttata cctacctct 
attgtaaag ttatcgtaa acttcatct ttaacgtgt 
tccaaattg ctggtattt ttttgtact tcttaaaaa 
tatatgtta tttaagaaa
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