Status
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Gene information
General information
TC010569 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451448.1: 128488..133707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010569
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04871

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    3 larva

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010569 RA
      MSEFEADTP VREKKKKKS KGDKDLGNL QKGSEFAVE 
      PSSVIAKLD SSKWPLLLK YFDRLNVRT NHYVPLPFG 
      SSPLRREIS DYVKSGYIN LDKPSNPSS HEVVAWIKR 
      ILKVEKTGH SGTLDPKVT GCLIVCIER ATRLVKSQQ 
      AAGKEYVAV FKLHSTVQG GTPKVLQGL EKLRGALFQ 
      RPPLISAVK RQLRVRTVY DSKLFQYDE SRNMGVFWV 
      SCEAGSYIR TMCVHLGLV LGVGGQMLE LRRVRSGIQ 
      SEQDQMATM HDVLDAQWL YENHKDENY LRRVIRPLE 
      GLLVKHKRI IMKDSSVNA VCYGAKILL PGILRYEDN 
      IELNEEIVI VTTKGEAIA LGIALMTTA TIASCDHGV 
      VAKIKRVIM ERDTYPRKW GLGPRTSQK KILIAKGQL 
      DKYGKPNEN TPKEWLNSY VDYSVKKED TKDDEDDKK 
      RKLADTSMQ SDTASPKEK KKKRKKVEG EESTVEEAT 
      AVSEETAED NGEEKKKKK KKKKDKSKD REEEEAE
    CDS sequence
    • >TC010569 RA
      atgtctgag tttgaggcg gatacgccc gttagggag 
      aagaaaaag aagaaaagc aagggcgac aaagacttg 
      gggaacctt caaaaaggc tccgaattt gcagtggag 
      ccctcctca gtgattgca aagttggac agttccaag 
      tggcccttg ttgttaaag tattttgac cgtttgaat 
      gtgagaacg aaccattac gtacctttg ccgtttggt 
      tcttcccct ttacgaagg gaaatttcc gattatgtt 
      aagtcaggt tacattaac ctggacaag cccagtaat 
      ccaagcagt cacgaagtt gtagcttgg attaaaagg 
      attttgaaa gtggaaaaa acaggtcat tcgggtact 
      ttagatccg aaagttaca ggttgttta attgtgtgc 
      attgaacgc gctaccaga ctggttaaa tcccagcaa 
      gctgcaggg aaagagtac gtggctgtg tttaaattg 
      cattcaact gtacaggga gggacccca aaagtgctc 
      caagggttg gagaagctc cgaggggcc ttgtttcaa 
      agaccccca ttaatttcg gccgttaag agacagttg 
      agggttcgc acggtctat gatagcaaa ttattccaa 
      tatgatgaa agccgaaat atgggcgtg ttttgggtt 
      agttgcgag gctggttcc tacattcgt acaatgtgt 
      gttcatttg ggtttggtt ttgggcgtt ggaggccaa 
      atgttggaa cttcgaaga gttcgttcg ggaattcag 
      agcgaacaa gatcaaatg gccacaatg catgatgtt 
      ttagacgct caatggctt tacgagaat cacaaagat 
      gagaattac ctgcgacga gtgattcgg cctttggaa 
      ggccttttg gtcaaacac aaaaggata attatgaaa 
      gacagctcg gtaaacgcg gtttgctat ggagctaaa 
      attttgcta ccgggtatt ttacgctat gaagacaat 
      attgaactt aacgaagaa attgttatt gtgaccact 
      aaaggggaa gccatagca ctagggatt gcactgatg 
      accactgca acaatagcc agttgtgac cacggagtt 
      gtcgcgaaa atcaaacgt gtgataatg gaacgagac 
      acttatcca agaaaatgg ggcttggga ccacgaaca 
      agccagaaa aaaattctt attgcaaaa ggacagctc 
      gacaaatac ggaaaaccg aacgaaaat acgcccaaa 
      gaatggcta aatagttac gttgattat tctgttaag 
      aaagaggac accaaggac gatgaagat gataaaaaa 
      cgtaaatta gcggacacg tcaatgcag tccgataca 
      gcgagtccg aaagagaag aagaagaaa cggaagaag 
      gtcgaaggt gaggaaagc actgttgaa gaagcgaca 
      gcagtgtca gaggaaact gctgaagat aatggggag 
      gaaaaaaag aaaaagaag aaaaagaaa aaggataag 
      agtaaagat agggaggaa gaggaagcg gaatga
    mRNA sequence
    • >TC010569 RA
      tctgtcaaa attgttgtt agttgtcaa aaaactaac 
      cctactttc tgtgtcgga tgcacgtgt tggttattt 
      tctaagcga tgtctgagt ttgaggcgg atacgcccg 
      ttagggaga agaaaaaga agaaaagca agggcgaca 
      aagacttgg ggaaccttc aaaaaggct ccgaatttg 
      cagtggagc cctcctcag tgattgcaa agttggaca 
      gttccaagt ggcccttgt tgttaaagt attttgacc 
      gtttgaatg tgagaacga accattacg tacctttgc 
      cgtttggtt cttcccctt tacgaaggg aaatttccg 
      attatgtta agtcaggtt acattaacc tggacaagc 
      ccagtaatc caagcagtc acgaagttg tagcttgga 
      ttaaaagga ttttgaaag tggaaaaaa caggtcatt 
      cgggtactt tagatccga aagttacag gttgtttaa 
      ttgtgtgca ttgaacgcg ctaccagac tggttaaat 
      cccagcaag ctgcaggga aagagtacg tggctgtgt 
      ttaaattgc attcaactg tacagggag ggaccccaa 
      aagtgctcc aagggttgg agaagctcc gaggggcct 
      tgtttcaaa gacccccat taatttcgg ccgttaaga 
      gacagttga gggttcgca cggtctatg atagcaaat 
      tattccaat atgatgaaa gccgaaata tgggcgtgt 
      tttgggtta gttgcgagg ctggttcct acattcgta 
      caatgtgtg ttcatttgg gtttggttt tgggcgttg 
      gaggccaaa tgttggaac ttcgaagag ttcgttcgg 
      gaattcaga gcgaacaag atcaaatgg ccacaatgc 
      atgatgttt tagacgctc aatggcttt acgagaatc 
      acaaagatg agaattacc tgcgacgag tgattcggc 
      ctttggaag gccttttgg tcaaacaca aaaggataa 
      ttatgaaag acagctcgg taaacgcgg tttgctatg 
      gagctaaaa ttttgctac cgggtattt tacgctatg 
      aagacaata ttgaactta acgaagaaa ttgttattg 
      tgaccacta aaggggaag ccatagcac tagggattg 
      cactgatga ccactgcaa caatagcca gttgtgacc 
      acggagttg tcgcgaaaa tcaaacgtg tgataatgg 
      aacgagaca cttatccaa gaaaatggg gcttgggac 
      cacgaacaa gccagaaaa aaattctta ttgcaaaag 
      gacagctcg acaaatacg gaaaaccga acgaaaata 
      cgcccaaag aatggctaa atagttacg ttgattatt 
      ctgttaaga aagaggaca ccaaggacg atgaagatg 
      ataaaaaac gtaaattag cggacacgt caatgcagt 
      ccgatacag cgagtccga aagagaaga agaagaaac 
      ggaagaagg tcgaaggtg aggaaagca ctgttgaag 
      aagcgacag cagtgtcag aggaaactg ctgaagata 
      atggggagg aaaaaaaga aaaagaaga aaaagaaaa 
      aggataaga gtaaagata gggaggaag aggaagcgg 
      aatgaacta ttttgtttt gcattttta tattgtagc 
      tctacaata ctacattaa tagaattaa gtttaaatt 
      ctttctaag ataaacaca gtcgtgtta agcgaaaat 
      aaatgcaat tttatcgta caggctttg atttttcca 
      gtttgatgt agtggtaca agtgcaaga aatttgtag 
      gtataccgg gtgtccagt ttggtaatt ctatatttt 
      ttttgaaat ggtacattt ttagtaata tttatgaca 
      ctggtattt gagtaatgg ttcattttt tattttttt 
      cgataatac tccatcttt caagtaagt ataaaacaa 
      tgaaatcaa aaaaatatt gataatggt tttactttt 
      ctgttttat atatttttt tcatagtct ggaggacta 
      ggttttttg atataaaac atgtactaa aaattgtta 
      aaaaattgt cacatttaa agaaattaa taaaagtag 
      ttgcctagt
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