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Gene information
General information
TC010549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15407094..15422568
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010549
    iBeetle screen iB_04868

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010549 RA
      MCDTPFKTP LPYRTPRAD LNRDMFVEK GASDSDESV 
      ASCNSDNMG SGSGQSSNG DISLAAQFD DLMRSYRPF 
      RNNELETLP AFMEFVEEV KLLYVDYFQ ALDEINRLK 
      NVLDNKVVE CSDLENKLH TARKMIDKE KLMVRTARK 
      ERDELASQI NLVKDLLFK DKNTTIRFS DEMRQKFSF 
      LHNNNWQSE WDLTSANGN GHHPQLSAI REVNSTGSM 
      LSDFSYSQS EDDLDHSRL SGVAWKKHR PSTGASAEP 
      AIKKRRSSG NKVVEIGAA DTVRATTTL TLNKDGPIT 
      ATSIIESVP KTPGAPESA HTADSYPSA PPANLVFES 
      WARESPRKT HQKLAGKEH CFQQKTLVM PETCTSCDK 
      RIRFGRSAF KCKECKALC HLECKNNLP LPCIPVVHT 
      PNQRNGLGV IGDYTPTTP PMVPAILIH CINEIELRG 
      LSEVGLYRV PGSERDVKA LKERFLKGK GAPCLNQVD 
      IPVICGTVK DFLRGLHEP VITYALWKK FVQAVNAQD 
      PLDIQPALY QAISELPQP NRDTLAYMI LHLQKVAEA 
      KECKMNIGN LAKIFGPTL VGYSSDNPN PNNLLDEIV 
      QHNMVMEHL LNIPSDYWS GFINIEPTY QRGKLQQTP 
      STDSLLRPI APKGLFTPV SANRSCAKK KQKYFPSPK 
      NFR
    CDS sequence
    • >TC010549 RA
      atgtgtgac actcctttt aaaacaccc ctaccgtat 
      cgaaccccc cgagctgac ttgaaccga gacatgttt 
      gtggaaaaa ggagcctcc gacagtgat gaaagcgtt 
      gcctcgtgt aattctgat aatatgggt tctggatca 
      ggccagtct tcaaacgga gacatttcc ctggcggcg 
      caatttgat gatttgatg cggagttat cgccccttt 
      agaaacaat gagttagag actctccca gcgtttatg 
      gagtttgtt gaagaagtg aagttattg tatgttgat 
      tattttcaa gcactggat gaaattaac aggctgaaa 
      aatgttctc gataataaa gtagtggaa tgtagtgat 
      ttggagaat aagttacac acagctcgg aaaatgatt 
      gataaggaa aagctaatg gttaggaca gccaggaag 
      gagagggat gaattagct tcacagata aaccttgtc 
      aaagacctt ctcttcaag gataaaaat actactatc 
      aggttttcg gatgaaatg aggcaaaaa ttttcattt 
      ttgcataat aataactgg caaagcgag tgggatttg 
      accagtgca aatggaaat ggtcatcat ccccaactt 
      agcgctatc agagaagtt aattcaact ggatccatg 
      ttgtcagac tttagctat tcacagtca gaagatgat 
      ttagatcat tctcgtctc tctggagtt gcttggaaa 
      aaacacagg ccaagcaca ggtgcctct gccgaacct 
      gcaattaaa aaaagacgt tcttctgga aataaagtt 
      gttgagatt ggagctgct gacaccgtt agagcaacc 
      actactcta actcttaac aaagatggt cctattact 
      gctacgtct attatcgag tccgtccct aaaactccg 
      ggagcccca gaatcggcc catacggcc gactcatac 
      cccagtgcc cctccagca aaccttgtt tttgaatcc 
      tgggctcgt gaaagtccg cgcaaaact caccagaaa 
      ctagccggc aaagaacat tgttttcaa caaaaaact 
      cttgtaatg cctgaaact tgcacatct tgcgataaa 
      cgaatccgc tttggtagg agcgctttt aagtgcaaa 
      gaatgcaaa gctctgtgt catttggaa tgcaagaac 
      aatcttcct ttgccatgt attcctgtg gtgcataca 
      ccaaatcaa cgaaacgga ttgggggtt attggtgat 
      tatacccca acaactccc cccatggtt ccagctatt 
      cttatccac tgcataaac gaaattgaa ttaagaggg 
      ctctcagaa gttggctta taccgagtt ccaggctca 
      gaacgtgat gttaaggct ttgaaggaa cggtttctg 
      aaaggcaaa ggggcccca tgtcttaat caagttgac 
      attcctgtg atttgtgga actgtcaaa gactttctc 
      aggggttta catgaacca gtcataact tacgccttg 
      tggaaaaaa tttgtacaa gctgtgaat gctcaggac 
      ccacttgac atccaacca gctctgtat caagccata 
      tcagagctt ccacaacca aacagagac actctcgca 
      tatatgatt ctgcatctt cagaaggtg gcagaggcc 
      aaagaatgc aaaatgaat atcggtaac cttgccaaa 
      attttcgga ccgacttta gttggatac tcgtccgat 
      aatccaaac ccgaataat ttgctggat gaaatcgtt 
      caacataat atggtaatg gaacatttg cttaatatc 
      ccatctgat tactggtct ggttttatc aacatcgag 
      ccgacttat caacgtgga aaattgcag caaactcca 
      agcactgat tcattattg cgtcctata gctcctaaa 
      ggacttttc acaccagtg agcgctaac agaagttgt 
      gcgaaaaag aagcagaag tattttcct tcacccaag 
      aatttccga taa
    mRNA sequence
    • >TC010549 RA
      gtgttggaa aaactccgg tgcaactta cctcttcct 
      ggtggcaat atttaggtc agcattgct cctgcaggg 
      agacacacc ctcgtttat tgactatca acgattgca 
      aactaataa ctacaacgg caggactgt ttgcacact 
      gacaccata ttacaccag acatacgcc agcactcgt 
      gcgaatcgc gcgagcttt tcgaaaatt cgcgagtgc 
      tttagcgaa aaaaaatcg gaaaaacgg agcggtttc 
      actaacacg atttattcg ggtttcaat tcctacgtc 
      ataattgaa aacgacaat taaagctca catcggcca 
      gtttataat taaatttgg tggtcctgc gatttacgt 
      tgttagatt ccggggact cgacacgga ctaaattca 
      ttcattgac tacactttt gcgcaagcg ctctttcac 
      ttcgccgtg ccatcgccg caggtatgg atcattacg 
      ctgcggtcg gttaaagta tgacagctg atcggtgag 
      ctccgattg gtcattttc aaaattgca tttcttcaa 
      ctgtaactt caaagaaca cgaagcttt aactctcat 
      atttgttag aattttagt gagtttctt gaggaacat 
      cgagtgttt ttgtgcttt agacacaga ttagagagg 
      ttagttgat ctgtgcttt agatgtgta acaagttga 
      gtttgagtt aagtgtgca gtgcaacta agaaaaaat 
      gtgtgacac tccttttaa aacacccct accgtatcg 
      aaccccccg agctgactt gaaccgaga catgtttgt 
      ggaaaaagg agcctccga cagtgatga aagcgttgc 
      ctcgtgtaa ttctgataa tatgggttc tggatcagg 
      ccagtcttc aaacggaga catttccct ggcggcgca 
      atttgatga tttgatgcg gagttatcg cccctttag 
      aaacaatga gttagagac tctcccagc gtttatgga 
      gtttgttga agaagtgaa gttattgta tgttgatta 
      ttttcaagc actggatga aattaacag gctgaaaaa 
      tgttctcga taataaagt agtggaatg tagtgattt 
      ggagaataa gttacacac agctcggaa aatgattga 
      taaggaaaa gctaatggt taggacagc caggaagga 
      gagggatga attagcttc acagataaa ccttgtcaa 
      agaccttct cttcaagga taaaaatac tactatcag 
      gttttcgga tgaaatgag gcaaaaatt ttcattttt 
      gcataataa taactggca aagcgagtg ggatttgac 
      cagtgcaaa tggaaatgg tcatcatcc ccaacttag 
      cgctatcag agaagttaa ttcaactgg atccatgtt 
      gtcagactt tagctattc acagtcaga agatgattt 
      agatcattc tcgtctctc tggagttgc ttggaaaaa 
      acacaggcc aagcacagg tgcctctgc cgaacctgc 
      aattaaaaa aagacgttc ttctggaaa taaagttgt 
      tgagattgg agctgctga caccgttag agcaaccac 
      tactctaac tcttaacaa agatggtcc tattactgc 
      tacgtctat tatcgagtc cgtccctaa aactccggg 
      agccccaga atcggccca tacggccga ctcataccc 
      cagtgcccc tccagcaaa ccttgtttt tgaatcctg 
      ggctcgtga aagtccgcg caaaactca ccagaaact 
      agccggcaa agaacattg ttttcaaca aaaaactct 
      tgtaatgcc tgaaacttg cacatcttg cgataaacg 
      aatccgctt tggtaggag cgcttttaa gtgcaaaga 
      atgcaaagc tctgtgtca tttggaatg caagaacaa 
      tcttccttt gccatgtat tcctgtggt gcatacacc 
      aaatcaacg aaacggatt gggggttat tggtgatta 
      taccccaac aactccccc catggttcc agctattct 
      tatccactg cataaacga aattgaatt aagagggct 
      ctcagaagt tggcttata ccgagttcc aggctcaga 
      acgtgatgt taaggcttt gaaggaacg gtttctgaa 
      aggcaaagg ggccccatg tcttaatca agttgacat 
      tcctgtgat ttgtggaac tgtcaaaga ctttctcag 
      gggtttaca tgaaccagt cataactta cgccttgtg 
      gaaaaaatt tgtacaagc tgtgaatgc tcaggaccc 
      acttgacat ccaaccagc tctgtatca agccatatc 
      agagcttcc acaaccaaa cagagacac tctcgcata 
      tatgattct gcatcttca gaaggtggc agaggccaa 
      agaatgcaa aatgaatat cggtaacct tgccaaaat 
      tttcggacc gactttagt tggatactc gtccgataa 
      tccaaaccc gaataattt gctggatga aatcgttca 
      acataatat ggtaatgga acatttgct taatatccc 
      atctgatta ctggtctgg ttttatcaa catcgagcc 
      gacttatca acgtggaaa attgcagca aactccaag 
      cactgattc attattgcg tcctatagc tcctaaagg 
      acttttcac accagtgag cgctaacag aagttgtgc 
      gaaaaagaa gcagaagta ttttccttc acccaagaa 
      tttccgata aattattac ttattaata agttttttt 
      acattttat actgaagaa atattattt tctaaactt 
      tagcatctt ttatatgcc ttttaaata taccactgt 
      atattgt
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