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Gene information
General information
TC010549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15407094..15422568
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010549
    iBeetle screen iB_04868

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010549 RA
      MCDTPFKTP LPYRTPRAD LNRDMFVEK GASDSDESV 
ASCNSDNMG SGSGQSSNG DISLAAQFD DLMRSYRPF 
RNNELETLP AFMEFVEEV KLLYVDYFQ ALDEINRLK 
NVLDNKVVE CSDLENKLH TARKMIDKE KLMVRTARK 
ERDELASQI NLVKDLLFK DKNTTIRFS DEMRQKFSF 
LHNNNWQSE WDLTSANGN GHHPQLSAI REVNSTGSM 
LSDFSYSQS EDDLDHSRL SGVAWKKHR PSTGASAEP 
AIKKRRSSG NKVVEIGAA DTVRATTTL TLNKDGPIT 
ATSIIESVP KTPGAPESA HTADSYPSA PPANLVFES 
WARESPRKT HQKLAGKEH CFQQKTLVM PETCTSCDK 
RIRFGRSAF KCKECKALC HLECKNNLP LPCIPVVHT 
PNQRNGLGV IGDYTPTTP PMVPAILIH CINEIELRG 
LSEVGLYRV PGSERDVKA LKERFLKGK GAPCLNQVD 
IPVICGTVK DFLRGLHEP VITYALWKK FVQAVNAQD 
PLDIQPALY QAISELPQP NRDTLAYMI LHLQKVAEA 
KECKMNIGN LAKIFGPTL VGYSSDNPN PNNLLDEIV 
QHNMVMEHL LNIPSDYWS GFINIEPTY QRGKLQQTP 
STDSLLRPI APKGLFTPV SANRSCAKK KQKYFPSPK 
NFR
    CDS sequence
    • >TC010549 RA
      atgtgtgac actcctttt aaaacaccc ctaccgtat 
cgaaccccc cgagctgac ttgaaccga gacatgttt 
gtggaaaaa ggagcctcc gacagtgat gaaagcgtt 
gcctcgtgt aattctgat aatatgggt tctggatca 
ggccagtct tcaaacgga gacatttcc ctggcggcg 
caatttgat gatttgatg cggagttat cgccccttt 
agaaacaat gagttagag actctccca gcgtttatg 
gagtttgtt gaagaagtg aagttattg tatgttgat 
tattttcaa gcactggat gaaattaac aggctgaaa 
aatgttctc gataataaa gtagtggaa tgtagtgat 
ttggagaat aagttacac acagctcgg aaaatgatt 
gataaggaa aagctaatg gttaggaca gccaggaag 
gagagggat gaattagct tcacagata aaccttgtc 
aaagacctt ctcttcaag gataaaaat actactatc 
aggttttcg gatgaaatg aggcaaaaa ttttcattt 
ttgcataat aataactgg caaagcgag tgggatttg 
accagtgca aatggaaat ggtcatcat ccccaactt 
agcgctatc agagaagtt aattcaact ggatccatg 
ttgtcagac tttagctat tcacagtca gaagatgat 
ttagatcat tctcgtctc tctggagtt gcttggaaa 
aaacacagg ccaagcaca ggtgcctct gccgaacct 
gcaattaaa aaaagacgt tcttctgga aataaagtt 
gttgagatt ggagctgct gacaccgtt agagcaacc 
actactcta actcttaac aaagatggt cctattact 
gctacgtct attatcgag tccgtccct aaaactccg 
ggagcccca gaatcggcc catacggcc gactcatac 
cccagtgcc cctccagca aaccttgtt tttgaatcc 
tgggctcgt gaaagtccg cgcaaaact caccagaaa 
ctagccggc aaagaacat tgttttcaa caaaaaact 
cttgtaatg cctgaaact tgcacatct tgcgataaa 
cgaatccgc tttggtagg agcgctttt aagtgcaaa 
gaatgcaaa gctctgtgt catttggaa tgcaagaac 
aatcttcct ttgccatgt attcctgtg gtgcataca 
ccaaatcaa cgaaacgga ttgggggtt attggtgat 
tatacccca acaactccc cccatggtt ccagctatt 
cttatccac tgcataaac gaaattgaa ttaagaggg 
ctctcagaa gttggctta taccgagtt ccaggctca 
gaacgtgat gttaaggct ttgaaggaa cggtttctg 
aaaggcaaa ggggcccca tgtcttaat caagttgac 
attcctgtg atttgtgga actgtcaaa gactttctc 
aggggttta catgaacca gtcataact tacgccttg 
tggaaaaaa tttgtacaa gctgtgaat gctcaggac 
ccacttgac atccaacca gctctgtat caagccata 
tcagagctt ccacaacca aacagagac actctcgca 
tatatgatt ctgcatctt cagaaggtg gcagaggcc 
aaagaatgc aaaatgaat atcggtaac cttgccaaa 
attttcgga ccgacttta gttggatac tcgtccgat 
aatccaaac ccgaataat ttgctggat gaaatcgtt 
caacataat atggtaatg gaacatttg cttaatatc 
ccatctgat tactggtct ggttttatc aacatcgag 
ccgacttat caacgtgga aaattgcag caaactcca 
agcactgat tcattattg cgtcctata gctcctaaa 
ggacttttc acaccagtg agcgctaac agaagttgt 
gcgaaaaag aagcagaag tattttcct tcacccaag 
aatttccga taa
    mRNA sequence
    • >TC010549 RA
      gtgttggaa aaactccgg tgcaactta cctcttcct 
ggtggcaat atttaggtc agcattgct cctgcaggg 
agacacacc ctcgtttat tgactatca acgattgca 
aactaataa ctacaacgg caggactgt ttgcacact 
gacaccata ttacaccag acatacgcc agcactcgt 
gcgaatcgc gcgagcttt tcgaaaatt cgcgagtgc 
tttagcgaa aaaaaatcg gaaaaacgg agcggtttc 
actaacacg atttattcg ggtttcaat tcctacgtc 
ataattgaa aacgacaat taaagctca catcggcca 
gtttataat taaatttgg tggtcctgc gatttacgt 
tgttagatt ccggggact cgacacgga ctaaattca 
ttcattgac tacactttt gcgcaagcg ctctttcac 
ttcgccgtg ccatcgccg caggtatgg atcattacg 
ctgcggtcg gttaaagta tgacagctg atcggtgag 
ctccgattg gtcattttc aaaattgca tttcttcaa 
ctgtaactt caaagaaca cgaagcttt aactctcat 
atttgttag aattttagt gagtttctt gaggaacat 
cgagtgttt ttgtgcttt agacacaga ttagagagg 
ttagttgat ctgtgcttt agatgtgta acaagttga 
gtttgagtt aagtgtgca gtgcaacta agaaaaaat 
gtgtgacac tccttttaa aacacccct accgtatcg 
aaccccccg agctgactt gaaccgaga catgtttgt 
ggaaaaagg agcctccga cagtgatga aagcgttgc 
ctcgtgtaa ttctgataa tatgggttc tggatcagg 
ccagtcttc aaacggaga catttccct ggcggcgca 
atttgatga tttgatgcg gagttatcg cccctttag 
aaacaatga gttagagac tctcccagc gtttatgga 
gtttgttga agaagtgaa gttattgta tgttgatta 
ttttcaagc actggatga aattaacag gctgaaaaa 
tgttctcga taataaagt agtggaatg tagtgattt 
ggagaataa gttacacac agctcggaa aatgattga 
taaggaaaa gctaatggt taggacagc caggaagga 
gagggatga attagcttc acagataaa ccttgtcaa 
agaccttct cttcaagga taaaaatac tactatcag 
gttttcgga tgaaatgag gcaaaaatt ttcattttt 
gcataataa taactggca aagcgagtg ggatttgac 
cagtgcaaa tggaaatgg tcatcatcc ccaacttag 
cgctatcag agaagttaa ttcaactgg atccatgtt 
gtcagactt tagctattc acagtcaga agatgattt 
agatcattc tcgtctctc tggagttgc ttggaaaaa 
acacaggcc aagcacagg tgcctctgc cgaacctgc 
aattaaaaa aagacgttc ttctggaaa taaagttgt 
tgagattgg agctgctga caccgttag agcaaccac 
tactctaac tcttaacaa agatggtcc tattactgc 
tacgtctat tatcgagtc cgtccctaa aactccggg 
agccccaga atcggccca tacggccga ctcataccc 
cagtgcccc tccagcaaa ccttgtttt tgaatcctg 
ggctcgtga aagtccgcg caaaactca ccagaaact 
agccggcaa agaacattg ttttcaaca aaaaactct 
tgtaatgcc tgaaacttg cacatcttg cgataaacg 
aatccgctt tggtaggag cgcttttaa gtgcaaaga 
atgcaaagc tctgtgtca tttggaatg caagaacaa 
tcttccttt gccatgtat tcctgtggt gcatacacc 
aaatcaacg aaacggatt gggggttat tggtgatta 
taccccaac aactccccc catggttcc agctattct 
tatccactg cataaacga aattgaatt aagagggct 
ctcagaagt tggcttata ccgagttcc aggctcaga 
acgtgatgt taaggcttt gaaggaacg gtttctgaa 
aggcaaagg ggccccatg tcttaatca agttgacat 
tcctgtgat ttgtggaac tgtcaaaga ctttctcag 
gggtttaca tgaaccagt cataactta cgccttgtg 
gaaaaaatt tgtacaagc tgtgaatgc tcaggaccc 
acttgacat ccaaccagc tctgtatca agccatatc 
agagcttcc acaaccaaa cagagacac tctcgcata 
tatgattct gcatcttca gaaggtggc agaggccaa 
agaatgcaa aatgaatat cggtaacct tgccaaaat 
tttcggacc gactttagt tggatactc gtccgataa 
tccaaaccc gaataattt gctggatga aatcgttca 
acataatat ggtaatgga acatttgct taatatccc 
atctgatta ctggtctgg ttttatcaa catcgagcc 
gacttatca acgtggaaa attgcagca aactccaag 
cactgattc attattgcg tcctatagc tcctaaagg 
acttttcac accagtgag cgctaacag aagttgtgc 
gaaaaagaa gcagaagta ttttccttc acccaagaa 
tttccgata aattattac ttattaata agttttttt 
acattttat actgaagaa atattattt tctaaactt 
tagcatctt ttatatgcc ttttaaata taccactgt 
atattgt
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