| General information | |||
| TC010549 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 15407094..15422568 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010549 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04868 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
- >TC010549 RA
MCDTPFKTP LPYRTPRAD LNRDMFVEK GASDSDESV ASCNSDNMG SGSGQSSNG DISLAAQFD DLMRSYRPF RNNELETLP AFMEFVEEV KLLYVDYFQ ALDEINRLK NVLDNKVVE CSDLENKLH TARKMIDKE KLMVRTARK ERDELASQI NLVKDLLFK DKNTTIRFS DEMRQKFSF LHNNNWQSE WDLTSANGN GHHPQLSAI REVNSTGSM LSDFSYSQS EDDLDHSRL SGVAWKKHR PSTGASAEP AIKKRRSSG NKVVEIGAA DTVRATTTL TLNKDGPIT ATSIIESVP KTPGAPESA HTADSYPSA PPANLVFES WARESPRKT HQKLAGKEH CFQQKTLVM PETCTSCDK RIRFGRSAF KCKECKALC HLECKNNLP LPCIPVVHT PNQRNGLGV IGDYTPTTP PMVPAILIH CINEIELRG LSEVGLYRV PGSERDVKA LKERFLKGK GAPCLNQVD IPVICGTVK DFLRGLHEP VITYALWKK FVQAVNAQD PLDIQPALY QAISELPQP NRDTLAYMI LHLQKVAEA KECKMNIGN LAKIFGPTL VGYSSDNPN PNNLLDEIV QHNMVMEHL LNIPSDYWS GFINIEPTY QRGKLQQTP STDSLLRPI APKGLFTPV SANRSCAKK KQKYFPSPK NFR
- >TC010549 RA
atgtgtgac actcctttt aaaacaccc ctaccgtat cgaaccccc cgagctgac ttgaaccga gacatgttt gtggaaaaa ggagcctcc gacagtgat gaaagcgtt gcctcgtgt aattctgat aatatgggt tctggatca ggccagtct tcaaacgga gacatttcc ctggcggcg caatttgat gatttgatg cggagttat cgccccttt agaaacaat gagttagag actctccca gcgtttatg gagtttgtt gaagaagtg aagttattg tatgttgat tattttcaa gcactggat gaaattaac aggctgaaa aatgttctc gataataaa gtagtggaa tgtagtgat ttggagaat aagttacac acagctcgg aaaatgatt gataaggaa aagctaatg gttaggaca gccaggaag gagagggat gaattagct tcacagata aaccttgtc aaagacctt ctcttcaag gataaaaat actactatc aggttttcg gatgaaatg aggcaaaaa ttttcattt ttgcataat aataactgg caaagcgag tgggatttg accagtgca aatggaaat ggtcatcat ccccaactt agcgctatc agagaagtt aattcaact ggatccatg ttgtcagac tttagctat tcacagtca gaagatgat ttagatcat tctcgtctc tctggagtt gcttggaaa aaacacagg ccaagcaca ggtgcctct gccgaacct gcaattaaa aaaagacgt tcttctgga aataaagtt gttgagatt ggagctgct gacaccgtt agagcaacc actactcta actcttaac aaagatggt cctattact gctacgtct attatcgag tccgtccct aaaactccg ggagcccca gaatcggcc catacggcc gactcatac cccagtgcc cctccagca aaccttgtt tttgaatcc tgggctcgt gaaagtccg cgcaaaact caccagaaa ctagccggc aaagaacat tgttttcaa caaaaaact cttgtaatg cctgaaact tgcacatct tgcgataaa cgaatccgc tttggtagg agcgctttt aagtgcaaa gaatgcaaa gctctgtgt catttggaa tgcaagaac aatcttcct ttgccatgt attcctgtg gtgcataca ccaaatcaa cgaaacgga ttgggggtt attggtgat tatacccca acaactccc cccatggtt ccagctatt cttatccac tgcataaac gaaattgaa ttaagaggg ctctcagaa gttggctta taccgagtt ccaggctca gaacgtgat gttaaggct ttgaaggaa cggtttctg aaaggcaaa ggggcccca tgtcttaat caagttgac attcctgtg atttgtgga actgtcaaa gactttctc aggggttta catgaacca gtcataact tacgccttg tggaaaaaa tttgtacaa gctgtgaat gctcaggac ccacttgac atccaacca gctctgtat caagccata tcagagctt ccacaacca aacagagac actctcgca tatatgatt ctgcatctt cagaaggtg gcagaggcc aaagaatgc aaaatgaat atcggtaac cttgccaaa attttcgga ccgacttta gttggatac tcgtccgat aatccaaac ccgaataat ttgctggat gaaatcgtt caacataat atggtaatg gaacatttg cttaatatc ccatctgat tactggtct ggttttatc aacatcgag ccgacttat caacgtgga aaattgcag caaactcca agcactgat tcattattg cgtcctata gctcctaaa ggacttttc acaccagtg agcgctaac agaagttgt gcgaaaaag aagcagaag tattttcct tcacccaag aatttccga taa
- >TC010549 RA
gtgttggaa aaactccgg tgcaactta cctcttcct ggtggcaat atttaggtc agcattgct cctgcaggg agacacacc ctcgtttat tgactatca acgattgca aactaataa ctacaacgg caggactgt ttgcacact gacaccata ttacaccag acatacgcc agcactcgt gcgaatcgc gcgagcttt tcgaaaatt cgcgagtgc tttagcgaa aaaaaatcg gaaaaacgg agcggtttc actaacacg atttattcg ggtttcaat tcctacgtc ataattgaa aacgacaat taaagctca catcggcca gtttataat taaatttgg tggtcctgc gatttacgt tgttagatt ccggggact cgacacgga ctaaattca ttcattgac tacactttt gcgcaagcg ctctttcac ttcgccgtg ccatcgccg caggtatgg atcattacg ctgcggtcg gttaaagta tgacagctg atcggtgag ctccgattg gtcattttc aaaattgca tttcttcaa ctgtaactt caaagaaca cgaagcttt aactctcat atttgttag aattttagt gagtttctt gaggaacat cgagtgttt ttgtgcttt agacacaga ttagagagg ttagttgat ctgtgcttt agatgtgta acaagttga gtttgagtt aagtgtgca gtgcaacta agaaaaaat gtgtgacac tccttttaa aacacccct accgtatcg aaccccccg agctgactt gaaccgaga catgtttgt ggaaaaagg agcctccga cagtgatga aagcgttgc ctcgtgtaa ttctgataa tatgggttc tggatcagg ccagtcttc aaacggaga catttccct ggcggcgca atttgatga tttgatgcg gagttatcg cccctttag aaacaatga gttagagac tctcccagc gtttatgga gtttgttga agaagtgaa gttattgta tgttgatta ttttcaagc actggatga aattaacag gctgaaaaa tgttctcga taataaagt agtggaatg tagtgattt ggagaataa gttacacac agctcggaa aatgattga taaggaaaa gctaatggt taggacagc caggaagga gagggatga attagcttc acagataaa ccttgtcaa agaccttct cttcaagga taaaaatac tactatcag gttttcgga tgaaatgag gcaaaaatt ttcattttt gcataataa taactggca aagcgagtg ggatttgac cagtgcaaa tggaaatgg tcatcatcc ccaacttag cgctatcag agaagttaa ttcaactgg atccatgtt gtcagactt tagctattc acagtcaga agatgattt agatcattc tcgtctctc tggagttgc ttggaaaaa acacaggcc aagcacagg tgcctctgc cgaacctgc aattaaaaa aagacgttc ttctggaaa taaagttgt tgagattgg agctgctga caccgttag agcaaccac tactctaac tcttaacaa agatggtcc tattactgc tacgtctat tatcgagtc cgtccctaa aactccggg agccccaga atcggccca tacggccga ctcataccc cagtgcccc tccagcaaa ccttgtttt tgaatcctg ggctcgtga aagtccgcg caaaactca ccagaaact agccggcaa agaacattg ttttcaaca aaaaactct tgtaatgcc tgaaacttg cacatcttg cgataaacg aatccgctt tggtaggag cgcttttaa gtgcaaaga atgcaaagc tctgtgtca tttggaatg caagaacaa tcttccttt gccatgtat tcctgtggt gcatacacc aaatcaacg aaacggatt gggggttat tggtgatta taccccaac aactccccc catggttcc agctattct tatccactg cataaacga aattgaatt aagagggct ctcagaagt tggcttata ccgagttcc aggctcaga acgtgatgt taaggcttt gaaggaacg gtttctgaa aggcaaagg ggccccatg tcttaatca agttgacat tcctgtgat ttgtggaac tgtcaaaga ctttctcag gggtttaca tgaaccagt cataactta cgccttgtg gaaaaaatt tgtacaagc tgtgaatgc tcaggaccc acttgacat ccaaccagc tctgtatca agccatatc agagcttcc acaaccaaa cagagacac tctcgcata tatgattct gcatcttca gaaggtggc agaggccaa agaatgcaa aatgaatat cggtaacct tgccaaaat tttcggacc gactttagt tggatactc gtccgataa tccaaaccc gaataattt gctggatga aatcgttca acataatat ggtaatgga acatttgct taatatccc atctgatta ctggtctgg ttttatcaa catcgagcc gacttatca acgtggaaa attgcagca aactccaag cactgattc attattgcg tcctatagc tcctaaagg acttttcac accagtgag cgctaacag aagttgtgc gaaaaagaa gcagaagta ttttccttc acccaagaa tttccgata aattattac ttattaata agttttttt acattttat actgaagaa atattattt tctaaactt tagcatctt ttatatgcc ttttaaata taccactgt atattgt
