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Gene information
General information
TC010543 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15000420..15013674
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010543
      iBeetle screen iB_04867

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010543 RA
        MKPITFENL RRLVGGGRK KREKESSFK RSDSFKRIS 
        IRKSYLDRG KKKHISKLE VATQTVAEE DLLKLIETT 
        KVDSCVQVD KSDLSGATY ETTKCTKSK KSDSKSGPG 
        QSVIAYGQW LKGIRKDDS AVVSGIKGS ERTVIYVPA 
        SDEVQPSSP IIQLSCSPV FRKKSPLSK RKSKQSSPT 
        LQRKESNDS AVEMFPWGD SSEVKKSPL FRRKSRQSP 
        APLMPDSGT EEMCSLSIS LGRIWMDAP QGMAPRSLE 
        MPKTSGSSA PAHHSLDSA LKDLRDDPI VINRLQKRP 
        TPPVARTLS STSNTTVST GLFSSKDSG FSFSTTAPK 
        LSDFNSHVP VSTSRGFFR KKKPKPKLS VSRDGYFKR 
        TSGALIETK RSSVRRRSS RKKKSKTKK NRNSTKTDL 
        YQVVVSRPP RSLRSLKLD PMIFVPPEK RKTSVTRKQ 
        SFKIGMREI RNFDPLDSA MYSRSVNES ADEGLYESL 
        PGEYDDLSD EQPLSGRIS RISLREDDY DLPSNDFAP 
        VSEESIASD EPNSSSSPY MVPGVSPVP RRRPVRRKK 
        SGLTHKKSI TYVVKPNIK RVSSTLRRP AKKLGE
      CDS sequence
      • >TC010543 RA
        atgaaacca attacgttc gaaaatctg cgccgactg 
        gtgggtgga ggtcggaaa aaacgcgaa aaagaatca 
        tcttttaaa cgaagtgat tcatttaag cgaatatcc 
        ataagaaag agttaccta gatagaggc aagaaaaaa 
        cacatctcg aagttggaa gtggccacg caaacagtc 
        gccgaagag gatctctta aagttaatc gaaacaacg 
        aaagtcgat tcgtgcgta caagtggac aaatccgac 
        ttgagtggc gccacttac gaaaccacc aagtgcacc 
        aagtcgaag aaaagtgat tcgaaaagt ggaccggga 
        caaagtgtt atcgcgtac ggtcagtgg ttgaaaggc 
        atacgcaaa gacgactcg gcggtggtg tctggaatc 
        aagggctct gaacgaact gttatttac gtcccagcc 
        tctgacgag gtgcagcca agctcccct atcatccag 
        ctgtcttgc tcaccagtt tttcggaaa aaatctccc 
        ttgtcgaaa cgcaagagt aaacaaagc agtccgact 
        ctgcagcgg aaggaaagc aatgactcc gccgtggag 
        atgtttccg tggggagat tcctccgag gtgaaaaaa 
        tcccccctg tttaggaga aaatcccga cagagtcct 
        gcacctcta atgcccgat tccgggaca gaggagatg 
        tgttccttg tcgataagt ttggggagg atctggatg 
        gacgccccc caaggcatg gccccgaga agcctcgaa 
        atgccgaaa acctccgga tcgtcagcg cctgcacac 
        cactctctg gacagtgcc ttgaaggac ttacgggac 
        gaccctatc gtgatcaac cgtctccaa aagagaccc 
        actcctcct gttgctcgg acattatca tcaaccagc 
        aacacgaca gtgtccacc ggtctcttc tcgagcaaa 
        gattccggt ttttctttt tcaacgaca gctcctaaa 
        ttaagtgat ttcaattcg cacgtgcca gtatctaca 
        tcccgtggt tttttccgg aagaaaaaa cccaaaccg 
        aagctctct gtcagcagg gacggctac ttcaagcgt 
        acaagtggc gctctaata gaaactaag cggagttca 
        gttcggcgg agaagcagc cgcaagaaa aagtcgaaa 
        acgaagaaa aaccgaaac tccaccaag accgacctc 
        tatcaagtg gtcgttagc cgtccgccc aggtcgtta 
        cgttcctta aaactggac cccatgata ttcgttccg 
        ccagaaaag cgaaaaact tccgtgacc cgaaagcag 
        tcatttaag atcggaatg cgtgaaata aggaatttc 
        gatcctctg gatagtgcg atgtattcg aggagtgtg 
        aacgagagc gccgacgag gggctctac gagagcctc 
        cccggggaa tacgacgac ctctccgac gagcagcct 
        ctcagtgga cgcatatca cgcatcagc ctacgcgag 
        gacgactac gacttgccc tctaatgac ttcgctccg 
        gtgtcggag gaatcgatc gccagcgat gagccgaac 
        tcttcttcg agcccttat atggtcccc ggagtgtcg 
        cccgtgccg aggaggcgg ccggtgcgc cggaagaag 
        tcaggtttg acgcataag aagagtatc acctacgtg 
        gtcaagcct aatattaag agagtttcg tcgacgttg 
        cgcagacct gccaagaaa ttaggtgag taa
      mRNA sequence
      • >TC010543 RA
        gcgttcttc tatcaacat cgaagagtt tgttgtgcg 
        ttagttgtg tgtttagtg atgtttttc acattttgg 
        acggtgttt gtgacggaa attcttctt tgtgcggtg 
        gaatgtgac cgaggattg tgcggtgtg tcttcaaga 
        ccctagatt atggttgag aacgactgt gcgttggac 
        tctccagtc gcaatgaaa ttggaggaa tggctgaac 
        gttgtgcat tcaaatgca aacccaccg aacgtgtgg 
        tcaagtaga agaattcca gtgttactc gagacctag 
        agcggaaag aagcaaaca tgaaaccaa ttacgttcg 
        aaaatctgc gccgactgg tgggtggag gtcggaaaa 
        aacgcgaaa aagaatcat cttttaaac gaagtgatt 
        catttaagc gaatatcca taagaaaga gttacctag 
        atagaggca agaaaaaac acatctcga agttggaag 
        tggccacgc aaacagtcg ccgaagagg atctcttaa 
        agttaatcg aaacaacga aagtcgatt cgtgcgtac 
        aagtggaca aatccgact tgagtggcg ccacttacg 
        aaaccacca agtgcacca agtcgaaga aaagtgatt 
        cgaaaagtg gaccgggac aaagtgtta tcgcgtacg 
        gtcagtggt tgaaaggca tacgcaaag acgactcgg 
        cggtggtgt ctggaatca agggctctg aacgaactg 
        ttatttacg tcccagcct ctgacgagg tgcagccaa 
        gctccccta tcatccagc tgtcttgct caccagttt 
        ttcggaaaa aatctccct tgtcgaaac gcaagagta 
        aacaaagca gtccgactc tgcagcgga aggaaagca 
        atgactccg ccgtggaga tgtttccgt ggggagatt 
        cctccgagg tgaaaaaat cccccctgt ttaggagaa 
        aatcccgac agagtcctg cacctctaa tgcccgatt 
        ccgggacag aggagatgt gttccttgt cgataagtt 
        tggggagga tctggatgg acgcccccc aaggcatgg 
        ccccgagaa gcctcgaaa tgccgaaaa cctccggat 
        cgtcagcgc ctgcacacc actctctgg acagtgcct 
        tgaaggact tacgggacg accctatcg tgatcaacc 
        gtctccaaa agagaccca ctcctcctg ttgctcgga 
        cattatcat caaccagca acacgacag tgtccaccg 
        gtctcttct cgagcaaag attccggtt tttcttttt 
        caacgacag ctcctaaat taagtgatt tcaattcgc 
        acgtgccag tatctacat cccgtggtt ttttccgga 
        agaaaaaac ccaaaccga agctctctg tcagcaggg 
        acggctact tcaagcgta caagtggcg ctctaatag 
        aaactaagc ggagttcag ttcggcgga gaagcagcc 
        gcaagaaaa agtcgaaaa cgaagaaaa accgaaact 
        ccaccaaga ccgacctct atcaagtgg tcgttagcc 
        gtccgccca ggtcgttac gttccttaa aactggacc 
        ccatgatat tcgttccgc cagaaaagc gaaaaactt 
        ccgtgaccc gaaagcagt catttaaga tcggaatgc 
        gtgaaataa ggaatttcg atcctctgg atagtgcga 
        tgtattcga ggagtgtga acgagagcg ccgacgagg 
        ggctctacg agagcctcc ccggggaat acgacgacc 
        tctccgacg agcagcctc tcagtggac gcatatcac 
        gcatcagcc tacgcgagg acgactacg acttgccct 
        ctaatgact tcgctccgg tgtcggagg aatcgatcg 
        ccagcgatg agccgaact cttcttcga gcccttata 
        tggtccccg gagtgtcgc ccgtgccga ggaggcggc 
        cggtgcgcc ggaagaagt caggtttga cgcataaga 
        agagtatca cctacgtgg tcaagccta atattaaga 
        gagtttcgt cgacgttgc gcagacctg ccaagaaat 
        taggtgagt aatattgga gaatttccc gagcgatct 
        tttatttct gacacggat tagttcgat tttaattaa 
        aaaagaatt aatctccaa caatcttcc attcgagtt 
        agtcgagtg ggatgtgca aggttgaag aaatgtatg 
        ttggaatg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY