General information | |||
TC010543 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 15000420..15013674 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC010543 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04867 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC010543 RA
MKPITFENL RRLVGGGRK KREKESSFK RSDSFKRIS IRKSYLDRG KKKHISKLE VATQTVAEE DLLKLIETT KVDSCVQVD KSDLSGATY ETTKCTKSK KSDSKSGPG QSVIAYGQW LKGIRKDDS AVVSGIKGS ERTVIYVPA SDEVQPSSP IIQLSCSPV FRKKSPLSK RKSKQSSPT LQRKESNDS AVEMFPWGD SSEVKKSPL FRRKSRQSP APLMPDSGT EEMCSLSIS LGRIWMDAP QGMAPRSLE MPKTSGSSA PAHHSLDSA LKDLRDDPI VINRLQKRP TPPVARTLS STSNTTVST GLFSSKDSG FSFSTTAPK LSDFNSHVP VSTSRGFFR KKKPKPKLS VSRDGYFKR TSGALIETK RSSVRRRSS RKKKSKTKK NRNSTKTDL YQVVVSRPP RSLRSLKLD PMIFVPPEK RKTSVTRKQ SFKIGMREI RNFDPLDSA MYSRSVNES ADEGLYESL PGEYDDLSD EQPLSGRIS RISLREDDY DLPSNDFAP VSEESIASD EPNSSSSPY MVPGVSPVP RRRPVRRKK SGLTHKKSI TYVVKPNIK RVSSTLRRP AKKLGE
- >TC010543 RA
atgaaacca attacgttc gaaaatctg cgccgactg gtgggtgga ggtcggaaa aaacgcgaa aaagaatca tcttttaaa cgaagtgat tcatttaag cgaatatcc ataagaaag agttaccta gatagaggc aagaaaaaa cacatctcg aagttggaa gtggccacg caaacagtc gccgaagag gatctctta aagttaatc gaaacaacg aaagtcgat tcgtgcgta caagtggac aaatccgac ttgagtggc gccacttac gaaaccacc aagtgcacc aagtcgaag aaaagtgat tcgaaaagt ggaccggga caaagtgtt atcgcgtac ggtcagtgg ttgaaaggc atacgcaaa gacgactcg gcggtggtg tctggaatc aagggctct gaacgaact gttatttac gtcccagcc tctgacgag gtgcagcca agctcccct atcatccag ctgtcttgc tcaccagtt tttcggaaa aaatctccc ttgtcgaaa cgcaagagt aaacaaagc agtccgact ctgcagcgg aaggaaagc aatgactcc gccgtggag atgtttccg tggggagat tcctccgag gtgaaaaaa tcccccctg tttaggaga aaatcccga cagagtcct gcacctcta atgcccgat tccgggaca gaggagatg tgttccttg tcgataagt ttggggagg atctggatg gacgccccc caaggcatg gccccgaga agcctcgaa atgccgaaa acctccgga tcgtcagcg cctgcacac cactctctg gacagtgcc ttgaaggac ttacgggac gaccctatc gtgatcaac cgtctccaa aagagaccc actcctcct gttgctcgg acattatca tcaaccagc aacacgaca gtgtccacc ggtctcttc tcgagcaaa gattccggt ttttctttt tcaacgaca gctcctaaa ttaagtgat ttcaattcg cacgtgcca gtatctaca tcccgtggt tttttccgg aagaaaaaa cccaaaccg aagctctct gtcagcagg gacggctac ttcaagcgt acaagtggc gctctaata gaaactaag cggagttca gttcggcgg agaagcagc cgcaagaaa aagtcgaaa acgaagaaa aaccgaaac tccaccaag accgacctc tatcaagtg gtcgttagc cgtccgccc aggtcgtta cgttcctta aaactggac cccatgata ttcgttccg ccagaaaag cgaaaaact tccgtgacc cgaaagcag tcatttaag atcggaatg cgtgaaata aggaatttc gatcctctg gatagtgcg atgtattcg aggagtgtg aacgagagc gccgacgag gggctctac gagagcctc cccggggaa tacgacgac ctctccgac gagcagcct ctcagtgga cgcatatca cgcatcagc ctacgcgag gacgactac gacttgccc tctaatgac ttcgctccg gtgtcggag gaatcgatc gccagcgat gagccgaac tcttcttcg agcccttat atggtcccc ggagtgtcg cccgtgccg aggaggcgg ccggtgcgc cggaagaag tcaggtttg acgcataag aagagtatc acctacgtg gtcaagcct aatattaag agagtttcg tcgacgttg cgcagacct gccaagaaa ttaggtgag taa
- >TC010543 RA
gcgttcttc tatcaacat cgaagagtt tgttgtgcg ttagttgtg tgtttagtg atgtttttc acattttgg acggtgttt gtgacggaa attcttctt tgtgcggtg gaatgtgac cgaggattg tgcggtgtg tcttcaaga ccctagatt atggttgag aacgactgt gcgttggac tctccagtc gcaatgaaa ttggaggaa tggctgaac gttgtgcat tcaaatgca aacccaccg aacgtgtgg tcaagtaga agaattcca gtgttactc gagacctag agcggaaag aagcaaaca tgaaaccaa ttacgttcg aaaatctgc gccgactgg tgggtggag gtcggaaaa aacgcgaaa aagaatcat cttttaaac gaagtgatt catttaagc gaatatcca taagaaaga gttacctag atagaggca agaaaaaac acatctcga agttggaag tggccacgc aaacagtcg ccgaagagg atctcttaa agttaatcg aaacaacga aagtcgatt cgtgcgtac aagtggaca aatccgact tgagtggcg ccacttacg aaaccacca agtgcacca agtcgaaga aaagtgatt cgaaaagtg gaccgggac aaagtgtta tcgcgtacg gtcagtggt tgaaaggca tacgcaaag acgactcgg cggtggtgt ctggaatca agggctctg aacgaactg ttatttacg tcccagcct ctgacgagg tgcagccaa gctccccta tcatccagc tgtcttgct caccagttt ttcggaaaa aatctccct tgtcgaaac gcaagagta aacaaagca gtccgactc tgcagcgga aggaaagca atgactccg ccgtggaga tgtttccgt ggggagatt cctccgagg tgaaaaaat cccccctgt ttaggagaa aatcccgac agagtcctg cacctctaa tgcccgatt ccgggacag aggagatgt gttccttgt cgataagtt tggggagga tctggatgg acgcccccc aaggcatgg ccccgagaa gcctcgaaa tgccgaaaa cctccggat cgtcagcgc ctgcacacc actctctgg acagtgcct tgaaggact tacgggacg accctatcg tgatcaacc gtctccaaa agagaccca ctcctcctg ttgctcgga cattatcat caaccagca acacgacag tgtccaccg gtctcttct cgagcaaag attccggtt tttcttttt caacgacag ctcctaaat taagtgatt tcaattcgc acgtgccag tatctacat cccgtggtt ttttccgga agaaaaaac ccaaaccga agctctctg tcagcaggg acggctact tcaagcgta caagtggcg ctctaatag aaactaagc ggagttcag ttcggcgga gaagcagcc gcaagaaaa agtcgaaaa cgaagaaaa accgaaact ccaccaaga ccgacctct atcaagtgg tcgttagcc gtccgccca ggtcgttac gttccttaa aactggacc ccatgatat tcgttccgc cagaaaagc gaaaaactt ccgtgaccc gaaagcagt catttaaga tcggaatgc gtgaaataa ggaatttcg atcctctgg atagtgcga tgtattcga ggagtgtga acgagagcg ccgacgagg ggctctacg agagcctcc ccggggaat acgacgacc tctccgacg agcagcctc tcagtggac gcatatcac gcatcagcc tacgcgagg acgactacg acttgccct ctaatgact tcgctccgg tgtcggagg aatcgatcg ccagcgatg agccgaact cttcttcga gcccttata tggtccccg gagtgtcgc ccgtgccga ggaggcggc cggtgcgcc ggaagaagt caggtttga cgcataaga agagtatca cctacgtgg tcaagccta atattaaga gagtttcgt cgacgttgc gcagacctg ccaagaaat taggtgagt aatattgga gaatttccc gagcgatct tttatttct gacacggat tagttcgat tttaattaa aaaagaatt aatctccaa caatcttcc attcgagtt agtcgagtg ggatgtgca aggttgaag aaatgtatg ttggaatg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
