Status
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Gene information
General information
TC032516
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010536,TC010537
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14800469..14836626
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032516
    iBeetle screen iB_04866

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032516 RA
      MSSRSCNLI CCQNVELEC CEMDVNIKE TEKIKRETP 
      TGILRTESG KSRRASVEF KFLKQGEPT SPQPSVDRF 
      SVTFAEDSV KSDSSKCNY YSPRVGRMD KVHFPQGTD 
      IDDSDEEDY SASVNAIIQ RRASTRKSR RRSSRRASS 
      PFSPDILLS NEGGRRRSS VFTTSSGDT AITMDEGME 
      AVSQEEIFE NIKLHKEVL SNVKMQPWN MRKKLKLVV 
      QAKAYIKQH EGALQERLA QSRSTRDML ARWNIYLIK 
      KWQHYRREL ANLSNWLVP WERKIKEIE SHFGSVVAS 
      YFLFLRWLF WVNVVLGIV LISFVAVPE YVAADRLTA 
      FPCEMWFKI SLLVCCTGR ISGVLYPIR SNFREAVSY 
      SPPEDEVTF HLYHRSNFS KDDQLFIGD IGSILNSRF 
      NPDLSTKII IHGWTHGRD VPWVVEMRE AMAELDLWN 
      IIVVDWAPL SHAIYTEAR IHTGVVSRQ LTSFCIFMA 
      QSTGIRMSS VHMIGHSMG AQIAASAGY KLQLVLNQT 
      LARISGLDP AAPLYEWPH VESLDEILD PGDASFVDV 
      IHTNGRHLG TIFPSGHVD YYPNGGQHQ PGCGFWVCS 
      HMRSCEFWT ASVKKPDLF KAYSFQSWD EYLAGNTDG 
      LIAVPMGIA ANPSIPYGS YFLETTDEE RAYLNTTTT 
      ILDSFRKRK KRQRR
    • >TC032516 RB
      MSSRSCNLI CCQNVELEC CEMDVNIKE TEKIKRETP 
      TGILRTESG KSRRASVEF KFLKQGEPT SPQPSVDRF 
      SVTFAEDSV KSDSSKCNY YSPRVGRMD KVHFPQGTV 
      DIDDSDEED YSASVNAII QRRASTRKS RRRSSRRAS 
      SPFSPDILL SNEGGRRRS SVFTTSSGD TAITMDEGM 
      EAVSQEEIF ENIKLHKEV LSNVKMQPW NMRKKLKLV 
      VQAKAYIKQ HEGALQERL AQSRSTRDM LARWNIYLI 
      KKWQHYRRE LANLSNWLV PWERKIKEI ESHFGSVVA 
      SYFLFLRWL FWVNVVLGI VLISFVAVP EYVAADRLT 
      AFPCEMWFK ISLLVCCTG RISGVLYPI RSNFREAVS 
      YSPPEDEVT FHLYHRSNF SKDDQLFIG DIGSILNSR 
      FNPDLSTKI IIHGWTHGR DVPWVVEMR EAMAELDLW 
      NIIVVDWAP LSHAIYTEA RIHTGVVSR QLTSFCIFM 
      AQSTGIRMS SVHMIGHSM GAQIAASAG YKLQLVLNQ 
      TLARISGLD PAAPLYEWP HVESLDEIL DPGDASFVD 
      VIHTNGRHL GTIFPSGHV DYYPNGGQH QPGCGFWVC 
      SHMRSCEFW TASVKKPDL FKAYSFQSW DEYLAGNTD 
      GLIAVPMGI AANPSIPYG SYFLETTDE ERAYLNTTT 
      TILDSFRKR KKRQRR
    CDS sequence
    • >TC032516 RA
      atgtccagt cgcagctgc aacttaatt tgttgtcaa 
      aacgtggag ctcgagtgc tgcgagatg gacgtcaac 
      atcaaagag acggaaaag atcaagagg gaaacgccg 
      acggggatt ctccggact gagtcgggg aagtccagg 
      cgcgcctcg gtcgaattt aaatttctc aaacagggc 
      gaaccaacg tcgcctcaa ccttccgtc gatcggttt 
      tcggttacg tttgctgag gattcggta aaaagtgat 
      agctccaag tgtaactac tacagtccc agagttggc 
      agaatggat aaggttcat tttccacaa ggcacagac 
      atagacgat tccgacgaa gaagactac tcagcaagc 
      gtcaacgcc ataatccaa agacgtgcg tccacaaga 
      aaatctcgt cgccgcagc agccgtcgc gctagctca 
      cctttcagc cccgacatt ttactaagc aatgagggc 
      ggccgcaga cgctcttct gttttcacc actagttcc 
      ggagacacc gccataaca atggacgag ggaatggag 
      gccgtcagt caggaggaa atatttgag aatatcaaa 
      ctgcataag gaggtctta agcaacgta aaaatgcaa 
      ccgtggaac atgcgaaag aaactgaaa cttgtcgtc 
      caagcaaaa gcctacatt aagcaacac gaaggcgcc 
      ttgcaggaa agactggcg caaagtcga tcgacgagg 
      gatatgttg gcgaggtgg aacatttat cttatcaaa 
      aagtggcaa cattaccgc cgcgagtta gccaatttg 
      tccaattgg ctggtgcct tgggaaagg aagataaaa 
      gagattgag tcgcatttt ggatcagtc gtggcgtct 
      tattttctc tttctgaga tggttgttt tgggttaac 
      gtcgtattg gggattgta ttaatttcc ttcgtcgct 
      gtgccagag tatgttgcc gcagatcgg cttacagca 
      tttccttgc gaaatgtgg tttaaaatc tctttgcta 
      gtgtgctgt actggcaga attagtgga gtgttgtac 
      cccattagg agcaatttc agggaagct gttagctac 
      tctccgccg gaggacgaa gtgactttt cacttgtat 
      caccgaagc aacttttca aaagatgac cagctcttc 
      atcggcgat attggatcg atcttgaat tctcgattt 
      aatccggat ttatctacc aagattatc attcacgga 
      tggactcac gggagagat gtgccatgg gtggtggag 
      atgagggaa gctatggcc gaactggac ttatggaac 
      attattgtg gtggattgg gcccccttg tcccacgcc 
      atttacacc gaagctcgc atccacacc ggtgtcgtc 
      agtcgtcag ttaacctcc ttttgcatc ttcatggcg 
      caatcaacc ggcatcagg atgtcatca gttcacatg 
      ataggccac agtatggga gcccaaatc gctgcatct 
      gcaggctac aaactccaa ctcgtccta aatcaaact 
      ctggccaga atttcagga ctagaccct gcggcccct 
      ttgtacgaa tggcctcat gtcgagtcc cttgatgaa 
      attctggac ccgggggac gcttccttc gtggacgtc 
      atccacacg aacgggaga cacctcggg accattttt 
      ccgtcgggg cacgtcgat tattaccca aacggcggc 
      caacaccag cccggatgc ggcttttgg gtctgcagc 
      cacatgcgg tcatgcgaa ttctggacg gcttccgta 
      aagaaacct gatttattc aaggcatat tcgttccag 
      tcctgggat gaatattta gcaggaaac acagatgga 
      ctgatcgca gtcccgatg ggaattgca gccaatccg 
      agtatacct tacggttca tattttctc gaaaccact 
      gacgaggag agggcttac ctcaacacc accactaca 
      attcttgac agcttcaga aagaggaag aaaagacag 
      aggagataa
    • >TC032516 RB
      atgtccagt cgcagctgc aacttaatt tgttgtcaa 
      aacgtggag ctcgagtgc tgcgagatg gacgtcaac 
      atcaaagag acggaaaag atcaagagg gaaacgccg 
      acggggatt ctccggact gagtcgggg aagtccagg 
      cgcgcctcg gtcgaattt aaatttctc aaacagggc 
      gaaccaacg tcgcctcaa ccttccgtc gatcggttt 
      tcggttacg tttgctgag gattcggta aaaagtgat 
      agctccaag tgtaactac tacagtccc agagttggc 
      agaatggat aaggttcat tttccacaa ggcacagta 
      gacatagac gattccgac gaagaagac tactcagca 
      agcgtcaac gccataatc caaagacgt gcgtccaca 
      agaaaatct cgtcgccgc agcagccgt cgcgctagc 
      tcacctttc agccccgac attttacta agcaatgag 
      ggcggccgc agacgctct tctgttttc accactagt 
      tccggagac accgccata acaatggac gagggaatg 
      gaggccgtc agtcaggag gaaatattt gagaatatc 
      aaactgcat aaggaggtc ttaagcaac gtaaaaatg 
      caaccgtgg aacatgcga aagaaactg aaacttgtc 
      gtccaagca aaagcctac attaagcaa cacgaaggc 
      gccttgcag gaaagactg gcgcaaagt cgatcgacg 
      agggatatg ttggcgagg tggaacatt tatcttatc 
      aaaaagtgg caacattac cgccgcgag ttagccaat 
      ttgtccaat tggctggtg ccttgggaa aggaagata 
      aaagagatt gagtcgcat tttggatca gtcgtggcg 
      tcttatttt ctctttctg agatggttg ttttgggtt 
      aacgtcgta ttggggatt gtattaatt tccttcgtc 
      gctgtgcca gagtatgtt gccgcagat cggcttaca 
      gcatttcct tgcgaaatg tggtttaaa atctctttg 
      ctagtgtgc tgtactggc agaattagt ggagtgttg 
      taccccatt aggagcaat ttcagggaa gctgttagc 
      tactctccg ccggaggac gaagtgact tttcacttg 
      tatcaccga agcaacttt tcaaaagat gaccagctc 
      ttcatcggc gatattgga tcgatcttg aattctcga 
      tttaatccg gatttatct accaagatt atcattcac 
      ggatggact cacgggaga gatgtgcca tgggtggtg 
      gagatgagg gaagctatg gccgaactg gacttatgg 
      aacattatt gtggtggat tgggccccc ttgtcccac 
      gccatttac accgaagct cgcatccac accggtgtc 
      gtcagtcgt cagttaacc tccttttgc atcttcatg 
      gcgcaatca accggcatc aggatgtca tcagttcac 
      atgataggc cacagtatg ggagcccaa atcgctgca 
      tctgcaggc tacaaactc caactcgtc ctaaatcaa 
      actctggcc agaatttca ggactagac cctgcggcc 
      cctttgtac gaatggcct catgtcgag tcccttgat 
      gaaattctg gacccgggg gacgcttcc ttcgtggac 
      gtcatccac acgaacggg agacacctc gggaccatt 
      tttccgtcg gggcacgtc gattattac ccaaacggc 
      ggccaacac cagcccgga tgcggcttt tgggtctgc 
      agccacatg cggtcatgc gaattctgg acggcttcc 
      gtaaagaaa cctgattta ttcaaggca tattcgttc 
      cagtcctgg gatgaatat ttagcagga aacacagat 
      ggactgatc gcagtcccg atgggaatt gcagccaat 
      ccgagtata ccttacggt tcatatttt ctcgaaacc 
      actgacgag gagagggct tacctcaac accaccact 
      acaattctt gacagcttc agaaagagg aagaaaaga 
      cagaggaga taa
    mRNA sequence
    • >TC032516 RA
      cgtgtacca aaaatattt tttttcatt tgtttttgt 
      tgaaattca ttgtagtag aataatatc caaagcaaa 
      tattggaca tcaatttta aatcaaaag atgagtgtt 
      ccctggacg agattgctg tatacgcca ctggttgta 
      tgcaaacag cgtggatta gtgaaggat gggggtggc 
      gacaatgta gatgtccag tcgcagctg caacttaat 
      ttgttgtca aaacgtgga gctcgagtg ctgcgagat 
      ggacgtcaa catcaaaga gacggaaaa gatcaagag 
      ggaaacgcc gacggggat tctccggac tgagtcggg 
      gaagtccag gcgcgcctc ggtcgaatt taaatttct 
      caaacaggg cgaaccaac gtcgcctca accttccgt 
      cgatcggtt ttcggttac gtttgctga ggattcggt 
      aaaaagtga tagctccaa gtgtaacta ctacagtcc 
      cagagttgg cagaatgga taaggttca ttttccaca 
      aggcacaga catagacga ttccgacga agaagacta 
      ctcagcaag cgtcaacgc cataatcca aagacgtgc 
      gtccacaag aaaatctcg tcgccgcag cagccgtcg 
      cgctagctc acctttcag ccccgacat tttactaag 
      caatgaggg cggccgcag acgctcttc tgttttcac 
      cactagttc cggagacac cgccataac aatggacga 
      gggaatgga ggccgtcag tcaggagga aatatttga 
      gaatatcaa actgcataa ggaggtctt aagcaacgt 
      aaaaatgca accgtggaa catgcgaaa gaaactgaa 
      acttgtcgt ccaagcaaa agcctacat taagcaaca 
      cgaaggcgc cttgcagga aagactggc gcaaagtcg 
      atcgacgag ggatatgtt ggcgaggtg gaacattta 
      tcttatcaa aaagtggca acattaccg ccgcgagtt 
      agccaattt gtccaattg gctggtgcc ttgggaaag 
      gaagataaa agagattga gtcgcattt tggatcagt 
      cgtggcgtc ttattttct ctttctgag atggttgtt 
      ttgggttaa cgtcgtatt ggggattgt attaatttc 
      cttcgtcgc tgtgccaga gtatgttgc cgcagatcg 
      gcttacagc atttccttg cgaaatgtg gtttaaaat 
      ctctttgct agtgtgctg tactggcag aattagtgg 
      agtgttgta ccccattag gagcaattt cagggaagc 
      tgttagcta ctctccgcc ggaggacga agtgacttt 
      tcacttgta tcaccgaag caacttttc aaaagatga 
      ccagctctt catcggcga tattggatc gatcttgaa 
      ttctcgatt taatccgga tttatctac caagattat 
      cattcacgg atggactca cgggagaga tgtgccatg 
      ggtggtgga gatgaggga agctatggc cgaactgga 
      cttatggaa cattattgt ggtggattg ggccccctt 
      gtcccacgc catttacac cgaagctcg catccacac 
      cggtgtcgt cagtcgtca gttaacctc cttttgcat 
      cttcatggc gcaatcaac cggcatcag gatgtcatc 
      agttcacat gataggcca cagtatggg agcccaaat 
      cgctgcatc tgcaggcta caaactcca actcgtcct 
      aaatcaaac tctggccag aatttcagg actagaccc 
      tgcggcccc tttgtacga atggcctca tgtcgagtc 
      ccttgatga aattctgga cccggggga cgcttcctt 
      cgtggacgt catccacac gaacgggag acacctcgg 
      gaccatttt tccgtcggg gcacgtcga ttattaccc 
      aaacggcgg ccaacacca gcccggatg cggcttttg 
      ggtctgcag ccacatgcg gtcatgcga attctggac 
      ggcttccgt aaagaaacc tgatttatt caaggcata 
      ttcgttcca gtcctggga tgaatattt agcaggaaa 
      cacagatgg actgatcgc agtcccgat gggaattgc 
      agccaatcc gagtatacc ttacggttc atattttct 
      cgaaaccac tgacgagga gagggctta cctcaacac 
      caccactac aattcttga cagcttcag aaagaggaa 
      gaaaagaca gaggagata aaatgaatt gcttgctta 
      tcctatatc tgtcacggt tttttattt atttagtga 
      tttgtattt tctcatttt tgttaccca ataaagtaa 
      ttaatagaa a
    • >TC032516 RB
      cgtgtacca aaaatattt tttttcatt tgtttttgt 
      tgaaattca ttgtagtag aataatatc caaagcaaa 
      tattggaca tcaatttta aatcaaaag atgagtgtt 
      ccctggacg agattgctg tatacgcca ctggttgta 
      tgcaaacag cgtggatta gtgaaggat gggggtggc 
      gacaatgta gatgtccag tcgcagctg caacttaat 
      ttgttgtca aaacgtgga gctcgagtg ctgcgagat 
      ggacgtcaa catcaaaga gacggaaaa gatcaagag 
      ggaaacgcc gacggggat tctccggac tgagtcggg 
      gaagtccag gcgcgcctc ggtcgaatt taaatttct 
      caaacaggg cgaaccaac gtcgcctca accttccgt 
      cgatcggtt ttcggttac gtttgctga ggattcggt 
      aaaaagtga tagctccaa gtgtaacta ctacagtcc 
      cagagttgg cagaatgga taaggttca ttttccaca 
      aggcacagt agacataga cgattccga cgaagaaga 
      ctactcagc aagcgtcaa cgccataat ccaaagacg 
      tgcgtccac aagaaaatc tcgtcgccg cagcagccg 
      tcgcgctag ctcaccttt cagccccga cattttact 
      aagcaatga gggcggccg cagacgctc ttctgtttt 
      caccactag ttccggaga caccgccat aacaatgga 
      cgagggaat ggaggccgt cagtcagga ggaaatatt 
      tgagaatat caaactgca taaggaggt cttaagcaa 
      cgtaaaaat gcaaccgtg gaacatgcg aaagaaact 
      gaaacttgt cgtccaagc aaaagccta cattaagca 
      acacgaagg cgccttgca ggaaagact ggcgcaaag 
      tcgatcgac gagggatat gttggcgag gtggaacat 
      ttatcttat caaaaagtg gcaacatta ccgccgcga 
      gttagccaa tttgtccaa ttggctggt gccttggga 
      aaggaagat aaaagagat tgagtcgca ttttggatc 
      agtcgtggc gtcttattt tctctttct gagatggtt 
      gttttgggt taacgtcgt attggggat tgtattaat 
      ttccttcgt cgctgtgcc agagtatgt tgccgcaga 
      tcggcttac agcatttcc ttgcgaaat gtggtttaa 
      aatctcttt gctagtgtg ctgtactgg cagaattag 
      tggagtgtt gtaccccat taggagcaa tttcaggga 
      agctgttag ctactctcc gccggagga cgaagtgac 
      ttttcactt gtatcaccg aagcaactt ttcaaaaga 
      tgaccagct cttcatcgg cgatattgg atcgatctt 
      gaattctcg atttaatcc ggatttatc taccaagat 
      tatcattca cggatggac tcacgggag agatgtgcc 
      atgggtggt ggagatgag ggaagctat ggccgaact 
      ggacttatg gaacattat tgtggtgga ttgggcccc 
      cttgtccca cgccattta caccgaagc tcgcatcca 
      caccggtgt cgtcagtcg tcagttaac ctccttttg 
      catcttcat ggcgcaatc aaccggcat caggatgtc 
      atcagttca catgatagg ccacagtat gggagccca 
      aatcgctgc atctgcagg ctacaaact ccaactcgt 
      cctaaatca aactctggc cagaatttc aggactaga 
      ccctgcggc ccctttgta cgaatggcc tcatgtcga 
      gtcccttga tgaaattct ggacccggg ggacgcttc 
      cttcgtgga cgtcatcca cacgaacgg gagacacct 
      cgggaccat ttttccgtc ggggcacgt cgattatta 
      cccaaacgg cggccaaca ccagcccgg atgcggctt 
      ttgggtctg cagccacat gcggtcatg cgaattctg 
      gacggcttc cgtaaagaa acctgattt attcaaggc 
      atattcgtt ccagtcctg ggatgaata tttagcagg 
      aaacacaga tggactgat cgcagtccc gatgggaat 
      tgcagccaa tccgagtat accttacgg ttcatattt 
      tctcgaaac cactgacga ggagagggc ttacctcaa 
      caccaccac tacaattct tgacagctt cagaaagag 
      gaagaaaag acagaggag ataaaatga attgcttgc 
      ttatcctat atctgtcac ggtttttta tttatttag 
      tgatttgta ttttctcat ttttgttac ccaataaag 
      taattaata gaaa
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