Status
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Gene information
General information
TC010534 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14738920..14785613
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010534
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04865

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010534 RA
      MLDVPRPTS RVEIVAAMR RIRYEFKAK GIKKKKVTV 
EVSVDGVKV TLRKKKKRK HWLDDGKPL LLSHPIYRI 
FYVSHDSQD LKIFSYIAR DGASNVFKC AVFKSNKKV 
YICEAERRD ATCQLHPNQ FVSGRPPEA SSPLCPNKM 
KLLRAFTFF KIMENSSSD HLTRLKMSG KDDLEMPDP 
EVVVKKKQD MLTKARNKL FNLGENLKT HKIDGDHHR 
KTVTQATID YIFKHCPKE KNHVYANSN IHTKRNLQK 
QNSVGAYPI KIEITQDSD EEKETIKRS RPATVCVDK 
VSEGRETAA LALERPRKK LSFREPEIV GRTKQEQQK 
THLEVVGLK RSSSYCNGI LHRSPSFED SDLESQAMR 
VVRTIGQAF EVCHKLSIN APENENLDQ DEQDTLTQD 
LLSDRLSDV TSDKPKKDL LSEGASDKM SLPPDDSSF 
RDYNETKSA KPQQLDILP PPPTTNTRK SPLTSAETY 
SSPHSDVLT SGSGSGGSN SLPPPGSAL SAHHELQLL 
REQLEQQSQ QTQAAMAQL QLAREQLAA EQSARLEAQ 
ARTHQLLIH NRELLDHIA ALVAHLQGG EKASQQQSP 
PHVTMPQQQ QQPGSGENY SSDHLSETA SLDNSSALQ 
ALGLNPQGL IENRAVTSC LPPSPLRTS YNPPGSVFN 
FSFPQVDAC YDPQLLQRL QNMGGYTPS YAYNYTQTL 
PYLPNLYNQ PLINNNYTL QSPTQKKLP SSPMPMRHS 
FAGSSPASE RRVSPARGG EPMYPGNHL QVQQAVQRQ 
PSPDRRDGQ FIKPLSQMG TLTATDPEG RVRVIVPVP 
ANSAEDTGT LMANLRLSD SLRVSDDRT LNGPPITRS 
TSEKVPNRS ELMSQVQRT MWARHTTK
    CDS sequence
    • >TC010534 RA
      atgttggac gtgcctcga ccgaccagc agggtggag 
atcgtggcg gccatgcga aggatacga tacgaattc 
aaggcaaag ggaattaag aagaagaag gtgacggtg 
gaggtctcg gtggatggt gttaaagtt acgctcagg 
aaaaagaag aagaggaaa cactggctg gacgatgga 
aagccgcta ctcctgtca catcccatc tatagaata 
ttttacgtg tcacacgat tctcaggac ttgaaaata 
tttagttat atagctagg gacggagct tccaatgtt 
tttaaatgt gcagtattc aaatcgaac aaaaaggtg 
tatatttgc gaggccgag cgtcgcgac gccacttgc 
caactccat cctaatcag ttcgttagc ggccggcca 
cctgaagca tcgagtccg ttgtgtccg aataaaatg 
aagctttta cgcgccttc acgttcttc aaaataatg 
gagaactcg tcgagcgac catctaaca cgcctcaag 
atgtctgga aaggatgac ctggaaatg ccggatccg 
gaagttgtc gtcaagaag aagcaagac atgctgacc 
aaagcacgg aacaagttg ttcaatttg ggtgagaac 
ttgaaaact cacaaaata gacggggac caccacagg 
aagacggtg actcaagcc accatcgac tacatcttc 
aaacattgc cccaaagag aagaaccac gtttatgcc 
aacagtaac atccacacc aagaggaat ctgcagaag 
cagaactcg gttggagct tatccgatc aaaatcgag 
atcactcaa gactccgat gaggaaaag gagactatc 
aaaagatca agaccggcc acggtttgt gttgacaaa 
gtgagtgaa gggcgggaa accgcagcg ttggccctg 
gaaaggccc cggaagaag ctttcgttc cgagagccg 
gaaatcgtg ggtcgcacc aagcaagag cagcagaag 
acccacttg gaggtggtg ggactgaag aggagttcg 
agctattgc aatggaatc ctgcaccga agccccagt 
ttcgaagac tctgacttg gagagccag gcaatgcgc 
gtggtcagg accataggt caagcgttc gaagtgtgc 
cataaactg tcgattaac gctcctgag aacgaaaac 
ttggatcag gacgaacaa gatacgctt actcaagac 
ttgctcagc gaccggtta agtgacgta acgagtgat 
aagccgaag aaagatttg ctctctgaa ggtgccagt 
gacaagatg tctcttcct cccgacgat tcgagtttc 
cgggactac aacgagacc aaatcggcc aaacctcaa 
cagctcgac attctcccg cctccacct accaccaac 
acccgaaaa tcacccttg acgtcagcg gaaacgtac 
tcttcgccg cacagcgac gtcctcacc agcgggagc 
ggcagcggc ggcagcaac tcgctgccg cctccagga 
agcgccctt tcggcgcat cacgagctg cagttgctc 
agagagcaa ttggaacag cagagtcag caaacgcaa 
gccgcaatg gcacaactg caactcgcg agggagcaa 
ctcgccgct gaacagagc gctcgtttg gaggcccaa 
gcccgaaca caccagctg ctcatccac aacagggaa 
ttgctcgat cacatagcc gccctcgtg gcgcacctc 
caagggggc gagaaagcc agtcaacag cagagccct 
ccgcacgtg acgatgccc cagcagcaa cagcagccg 
gggtcaggc gaaaactac tcctcggac cacctctcc 
gaaaccgct tcactggac aacagtagc gctttacaa 
gccttgggc ttaaacccc caaggcctg atcgaaaac 
cgagcagta acatcgtgt ctaccacct tcgcccctc 
cggacatcc tacaaccca cccggcagc gtgttcaac 
ttttccttc ccccaagtt gacgcctgc tacgacccc 
cagctcctc caaagactc cagaacatg ggcggctac 
accccttca tacgcctac aactacacc cagaccctc 
ccctacttg cccaacctg tacaaccaa cccctcatc 
aacaacaac tacaccctg cagtcgccg actcagaag 
aagctgccg tcgtcaccc atgcccatg aggcactcc 
ttcgccggg agcagcccc gcgtcggag cgccgggtg 
agccccgcc agagggggc gagcccatg tacccgggg 
aaccacctg caggtgcag caggcggtg cagagacag 
ccgagtccc gacagaagg gatggccag ttcatcaag 
cccctgtcc cagatgggc accttgacg gccactgat 
cccgaggga agggtgcgg gtgatagtt ccagtcccg 
gcgaactcg gcggaagac accgggacg ctgatggcc 
aacttgaga ctgagtgac agtttgagg gttagtgac 
gatcggact ctgaacgga ccgccgatc acacggtcg 
acgagtgag aaagtgccc aacaggagc gaactgatg 
tcgcaagtg cagcgcacc atgtgggca agacacacc 
accaagtga
    mRNA sequence
    • >TC010534 RA
      ttattttta tgtttattg aaattgtgt ttttatttt 
cagtatatc gggatgttg gacgtgcct cgaccgacc 
agcagggtg gagatcgtg gcggccatg cgaaggata 
cgatacgaa ttcaaggca aagggaatt aagaagaag 
aaggtgacg gtggaggtc tcggtggat ggtgttaaa 
gttacgctc aggaaaaag aagaagagg aaacactgg 
ctggacgat ggaaagccg ctactcctg tcacatccc 
atctataga atattttac gtgtcacac gattctcag 
gacttgaaa atatttagt tatatagct agggacgga 
gcttccaat gtttttaaa tgtgcagta ttcaaatcg 
aacaaaaag gtgtatatt tgcgaggcc gagcgtcgc 
gacgccact tgccaactc catcctaat cagttcgtt 
agcggccgg ccacctgaa gcatcgagt ccgttgtgt 
ccgaataaa atgaagctt ttacgcgcc ttcacgttc 
ttcaaaata atggagaac tcgtcgagc gaccatcta 
acacgcctc aagatgtct ggaaaggat gacctggaa 
atgccggat ccggaagtt gtcgtcaag aagaagcaa 
gacatgctg accaaagca cggaacaag ttgttcaat 
ttgggtgag aacttgaaa actcacaaa atagacggg 
gaccaccac aggaagacg gtgactcaa gccaccatc 
gactacatc ttcaaacat tgccccaaa gagaagaac 
cacgtttat gccaacagt aacatccac accaagagg 
aatctgcag aagcagaac tcggttgga gcttatccg 
atcaaaatc gagatcact caagactcc gatgaggaa 
aaggagact atcaaaaga tcaagaccg gccacggtt 
tgtgttgac aaagtgagt gaagggcgg gaaaccgca 
gcgttggcc ctggaaagg ccccggaag aagctttcg 
ttccgagag ccggaaatc gtgggtcgc accaagcaa 
gagcagcag aagacccac ttggaggtg gtgggactg 
aagaggagt tcgagctat tgcaatgga atcctgcac 
cgaagcccc agtttcgaa gactctgac ttggagagc 
caggcaatg cgcgtggtc aggaccata ggtcaagcg 
ttcgaagtg tgccataaa ctgtcgatt aacgctcct 
gagaacgaa aacttggat caggacgaa caagatacg 
cttactcaa gacttgctc agcgaccgg ttaagtgac 
gtaacgagt gataagccg aagaaagat ttgctctct 
gaaggtgcc agtgacaag atgtctctt cctcccgac 
gattcgagt ttccgggac tacaacgag accaaatcg 
gccaaacct caacagctc gacattctc ccgcctcca 
cctaccacc aacacccga aaatcaccc ttgacgtca 
gcggaaacg tactcttcg ccgcacagc gacgtcctc 
accagcggg agcggcagc ggcggcagc aactcgctg 
ccgcctcca ggaagcgcc ctttcggcg catcacgag 
ctgcagttg ctcagagag caattggaa cagcagagt 
cagcaaacg caagccgca atggcacaa ctgcaactc 
gcgagggag caactcgcc gctgaacag agcgctcgt 
ttggaggcc caagcccga acacaccag ctgctcatc 
cacaacagg gaattgctc gatcacata gccgccctc 
gtggcgcac ctccaaggg ggcgagaaa gccagtcaa 
cagcagagc cctccgcac gtgacgatg ccccagcag 
caacagcag ccggggtca ggcgaaaac tactcctcg 
gaccacctc tccgaaacc gcttcactg gacaacagt 
agcgcttta caagccttg ggcttaaac ccccaaggc 
ctgatcgaa aaccgagca gtaacatcg tgtctacca 
ccttcgccc ctccggaca tcctacaac ccacccggc 
agcgtgttc aacttttcc ttcccccaa gttgacgcc 
tgctacgac ccccagctc ctccaaaga ctccagaac 
atgggcggc tacacccct tcatacgcc tacaactac 
acccagacc ctcccctac ttgcccaac ctgtacaac 
caacccctc atcaacaac aactacacc ctgcagtcg 
ccgactcag aagaagctg ccgtcgtca cccatgccc 
atgaggcac tccttcgcc gggagcagc cccgcgtcg 
gagcgccgg gtgagcccc gccagaggg ggcgagccc 
atgtacccg gggaaccac ctgcaggtg cagcaggcg 
gtgcagaga cagccgagt cccgacaga agggatggc 
cagttcatc aagcccctg tcccagatg ggcaccttg 
acggccact gatcccgag ggaagggtg cgggtgata 
gttccagtc ccggcgaac tcggcggaa gacaccggg 
acgctgatg gccaacttg agactgagt gacagtttg 
agggttagt gacgatcgg actctgaac ggaccgccg 
atcacacgg tcgacgagt gagaaagtg cccaacagg 
agcgaactg atgtcgcaa gtgcagcgc accatgtgg 
gcaagacac accaccaag tgatcatga catatcaga 
gttgcgatc gttaactta aggactaat tatagcatt 
ttgttttgt ttttcgtcg gtttttgtt tataaaaca 
cgtccagaa ctgctacca gatcgtagg aatacaagt 
aaatttagg cttgtttat attaggttc taatatcat 
ttacttgat aagcagttg tagaggtgt tagtaggaa 
aaatcagat cagtgttcg atatttttt ctagtcaaa 
ctatattta cttcaaggt ttttcgaaa aacaaacaa 
aaacggttt acggttaat acattttat ttaaaaat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY