Status
Please wait ...
Gene information
General information
TC010534 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14738920..14785613
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010534
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04865

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010534 RA
      MLDVPRPTS RVEIVAAMR RIRYEFKAK GIKKKKVTV 
      EVSVDGVKV TLRKKKKRK HWLDDGKPL LLSHPIYRI 
      FYVSHDSQD LKIFSYIAR DGASNVFKC AVFKSNKKV 
      YICEAERRD ATCQLHPNQ FVSGRPPEA SSPLCPNKM 
      KLLRAFTFF KIMENSSSD HLTRLKMSG KDDLEMPDP 
      EVVVKKKQD MLTKARNKL FNLGENLKT HKIDGDHHR 
      KTVTQATID YIFKHCPKE KNHVYANSN IHTKRNLQK 
      QNSVGAYPI KIEITQDSD EEKETIKRS RPATVCVDK 
      VSEGRETAA LALERPRKK LSFREPEIV GRTKQEQQK 
      THLEVVGLK RSSSYCNGI LHRSPSFED SDLESQAMR 
      VVRTIGQAF EVCHKLSIN APENENLDQ DEQDTLTQD 
      LLSDRLSDV TSDKPKKDL LSEGASDKM SLPPDDSSF 
      RDYNETKSA KPQQLDILP PPPTTNTRK SPLTSAETY 
      SSPHSDVLT SGSGSGGSN SLPPPGSAL SAHHELQLL 
      REQLEQQSQ QTQAAMAQL QLAREQLAA EQSARLEAQ 
      ARTHQLLIH NRELLDHIA ALVAHLQGG EKASQQQSP 
      PHVTMPQQQ QQPGSGENY SSDHLSETA SLDNSSALQ 
      ALGLNPQGL IENRAVTSC LPPSPLRTS YNPPGSVFN 
      FSFPQVDAC YDPQLLQRL QNMGGYTPS YAYNYTQTL 
      PYLPNLYNQ PLINNNYTL QSPTQKKLP SSPMPMRHS 
      FAGSSPASE RRVSPARGG EPMYPGNHL QVQQAVQRQ 
      PSPDRRDGQ FIKPLSQMG TLTATDPEG RVRVIVPVP 
      ANSAEDTGT LMANLRLSD SLRVSDDRT LNGPPITRS 
      TSEKVPNRS ELMSQVQRT MWARHTTK
    CDS sequence
    • >TC010534 RA
      atgttggac gtgcctcga ccgaccagc agggtggag 
      atcgtggcg gccatgcga aggatacga tacgaattc 
      aaggcaaag ggaattaag aagaagaag gtgacggtg 
      gaggtctcg gtggatggt gttaaagtt acgctcagg 
      aaaaagaag aagaggaaa cactggctg gacgatgga 
      aagccgcta ctcctgtca catcccatc tatagaata 
      ttttacgtg tcacacgat tctcaggac ttgaaaata 
      tttagttat atagctagg gacggagct tccaatgtt 
      tttaaatgt gcagtattc aaatcgaac aaaaaggtg 
      tatatttgc gaggccgag cgtcgcgac gccacttgc 
      caactccat cctaatcag ttcgttagc ggccggcca 
      cctgaagca tcgagtccg ttgtgtccg aataaaatg 
      aagctttta cgcgccttc acgttcttc aaaataatg 
      gagaactcg tcgagcgac catctaaca cgcctcaag 
      atgtctgga aaggatgac ctggaaatg ccggatccg 
      gaagttgtc gtcaagaag aagcaagac atgctgacc 
      aaagcacgg aacaagttg ttcaatttg ggtgagaac 
      ttgaaaact cacaaaata gacggggac caccacagg 
      aagacggtg actcaagcc accatcgac tacatcttc 
      aaacattgc cccaaagag aagaaccac gtttatgcc 
      aacagtaac atccacacc aagaggaat ctgcagaag 
      cagaactcg gttggagct tatccgatc aaaatcgag 
      atcactcaa gactccgat gaggaaaag gagactatc 
      aaaagatca agaccggcc acggtttgt gttgacaaa 
      gtgagtgaa gggcgggaa accgcagcg ttggccctg 
      gaaaggccc cggaagaag ctttcgttc cgagagccg 
      gaaatcgtg ggtcgcacc aagcaagag cagcagaag 
      acccacttg gaggtggtg ggactgaag aggagttcg 
      agctattgc aatggaatc ctgcaccga agccccagt 
      ttcgaagac tctgacttg gagagccag gcaatgcgc 
      gtggtcagg accataggt caagcgttc gaagtgtgc 
      cataaactg tcgattaac gctcctgag aacgaaaac 
      ttggatcag gacgaacaa gatacgctt actcaagac 
      ttgctcagc gaccggtta agtgacgta acgagtgat 
      aagccgaag aaagatttg ctctctgaa ggtgccagt 
      gacaagatg tctcttcct cccgacgat tcgagtttc 
      cgggactac aacgagacc aaatcggcc aaacctcaa 
      cagctcgac attctcccg cctccacct accaccaac 
      acccgaaaa tcacccttg acgtcagcg gaaacgtac 
      tcttcgccg cacagcgac gtcctcacc agcgggagc 
      ggcagcggc ggcagcaac tcgctgccg cctccagga 
      agcgccctt tcggcgcat cacgagctg cagttgctc 
      agagagcaa ttggaacag cagagtcag caaacgcaa 
      gccgcaatg gcacaactg caactcgcg agggagcaa 
      ctcgccgct gaacagagc gctcgtttg gaggcccaa 
      gcccgaaca caccagctg ctcatccac aacagggaa 
      ttgctcgat cacatagcc gccctcgtg gcgcacctc 
      caagggggc gagaaagcc agtcaacag cagagccct 
      ccgcacgtg acgatgccc cagcagcaa cagcagccg 
      gggtcaggc gaaaactac tcctcggac cacctctcc 
      gaaaccgct tcactggac aacagtagc gctttacaa 
      gccttgggc ttaaacccc caaggcctg atcgaaaac 
      cgagcagta acatcgtgt ctaccacct tcgcccctc 
      cggacatcc tacaaccca cccggcagc gtgttcaac 
      ttttccttc ccccaagtt gacgcctgc tacgacccc 
      cagctcctc caaagactc cagaacatg ggcggctac 
      accccttca tacgcctac aactacacc cagaccctc 
      ccctacttg cccaacctg tacaaccaa cccctcatc 
      aacaacaac tacaccctg cagtcgccg actcagaag 
      aagctgccg tcgtcaccc atgcccatg aggcactcc 
      ttcgccggg agcagcccc gcgtcggag cgccgggtg 
      agccccgcc agagggggc gagcccatg tacccgggg 
      aaccacctg caggtgcag caggcggtg cagagacag 
      ccgagtccc gacagaagg gatggccag ttcatcaag 
      cccctgtcc cagatgggc accttgacg gccactgat 
      cccgaggga agggtgcgg gtgatagtt ccagtcccg 
      gcgaactcg gcggaagac accgggacg ctgatggcc 
      aacttgaga ctgagtgac agtttgagg gttagtgac 
      gatcggact ctgaacgga ccgccgatc acacggtcg 
      acgagtgag aaagtgccc aacaggagc gaactgatg 
      tcgcaagtg cagcgcacc atgtgggca agacacacc 
      accaagtga
    mRNA sequence
    • >TC010534 RA
      ttattttta tgtttattg aaattgtgt ttttatttt 
      cagtatatc gggatgttg gacgtgcct cgaccgacc 
      agcagggtg gagatcgtg gcggccatg cgaaggata 
      cgatacgaa ttcaaggca aagggaatt aagaagaag 
      aaggtgacg gtggaggtc tcggtggat ggtgttaaa 
      gttacgctc aggaaaaag aagaagagg aaacactgg 
      ctggacgat ggaaagccg ctactcctg tcacatccc 
      atctataga atattttac gtgtcacac gattctcag 
      gacttgaaa atatttagt tatatagct agggacgga 
      gcttccaat gtttttaaa tgtgcagta ttcaaatcg 
      aacaaaaag gtgtatatt tgcgaggcc gagcgtcgc 
      gacgccact tgccaactc catcctaat cagttcgtt 
      agcggccgg ccacctgaa gcatcgagt ccgttgtgt 
      ccgaataaa atgaagctt ttacgcgcc ttcacgttc 
      ttcaaaata atggagaac tcgtcgagc gaccatcta 
      acacgcctc aagatgtct ggaaaggat gacctggaa 
      atgccggat ccggaagtt gtcgtcaag aagaagcaa 
      gacatgctg accaaagca cggaacaag ttgttcaat 
      ttgggtgag aacttgaaa actcacaaa atagacggg 
      gaccaccac aggaagacg gtgactcaa gccaccatc 
      gactacatc ttcaaacat tgccccaaa gagaagaac 
      cacgtttat gccaacagt aacatccac accaagagg 
      aatctgcag aagcagaac tcggttgga gcttatccg 
      atcaaaatc gagatcact caagactcc gatgaggaa 
      aaggagact atcaaaaga tcaagaccg gccacggtt 
      tgtgttgac aaagtgagt gaagggcgg gaaaccgca 
      gcgttggcc ctggaaagg ccccggaag aagctttcg 
      ttccgagag ccggaaatc gtgggtcgc accaagcaa 
      gagcagcag aagacccac ttggaggtg gtgggactg 
      aagaggagt tcgagctat tgcaatgga atcctgcac 
      cgaagcccc agtttcgaa gactctgac ttggagagc 
      caggcaatg cgcgtggtc aggaccata ggtcaagcg 
      ttcgaagtg tgccataaa ctgtcgatt aacgctcct 
      gagaacgaa aacttggat caggacgaa caagatacg 
      cttactcaa gacttgctc agcgaccgg ttaagtgac 
      gtaacgagt gataagccg aagaaagat ttgctctct 
      gaaggtgcc agtgacaag atgtctctt cctcccgac 
      gattcgagt ttccgggac tacaacgag accaaatcg 
      gccaaacct caacagctc gacattctc ccgcctcca 
      cctaccacc aacacccga aaatcaccc ttgacgtca 
      gcggaaacg tactcttcg ccgcacagc gacgtcctc 
      accagcggg agcggcagc ggcggcagc aactcgctg 
      ccgcctcca ggaagcgcc ctttcggcg catcacgag 
      ctgcagttg ctcagagag caattggaa cagcagagt 
      cagcaaacg caagccgca atggcacaa ctgcaactc 
      gcgagggag caactcgcc gctgaacag agcgctcgt 
      ttggaggcc caagcccga acacaccag ctgctcatc 
      cacaacagg gaattgctc gatcacata gccgccctc 
      gtggcgcac ctccaaggg ggcgagaaa gccagtcaa 
      cagcagagc cctccgcac gtgacgatg ccccagcag 
      caacagcag ccggggtca ggcgaaaac tactcctcg 
      gaccacctc tccgaaacc gcttcactg gacaacagt 
      agcgcttta caagccttg ggcttaaac ccccaaggc 
      ctgatcgaa aaccgagca gtaacatcg tgtctacca 
      ccttcgccc ctccggaca tcctacaac ccacccggc 
      agcgtgttc aacttttcc ttcccccaa gttgacgcc 
      tgctacgac ccccagctc ctccaaaga ctccagaac 
      atgggcggc tacacccct tcatacgcc tacaactac 
      acccagacc ctcccctac ttgcccaac ctgtacaac 
      caacccctc atcaacaac aactacacc ctgcagtcg 
      ccgactcag aagaagctg ccgtcgtca cccatgccc 
      atgaggcac tccttcgcc gggagcagc cccgcgtcg 
      gagcgccgg gtgagcccc gccagaggg ggcgagccc 
      atgtacccg gggaaccac ctgcaggtg cagcaggcg 
      gtgcagaga cagccgagt cccgacaga agggatggc 
      cagttcatc aagcccctg tcccagatg ggcaccttg 
      acggccact gatcccgag ggaagggtg cgggtgata 
      gttccagtc ccggcgaac tcggcggaa gacaccggg 
      acgctgatg gccaacttg agactgagt gacagtttg 
      agggttagt gacgatcgg actctgaac ggaccgccg 
      atcacacgg tcgacgagt gagaaagtg cccaacagg 
      agcgaactg atgtcgcaa gtgcagcgc accatgtgg 
      gcaagacac accaccaag tgatcatga catatcaga 
      gttgcgatc gttaactta aggactaat tatagcatt 
      ttgttttgt ttttcgtcg gtttttgtt tataaaaca 
      cgtccagaa ctgctacca gatcgtagg aatacaagt 
      aaatttagg cttgtttat attaggttc taatatcat 
      ttacttgat aagcagttg tagaggtgt tagtaggaa 
      aaatcagat cagtgttcg atatttttt ctagtcaaa 
      ctatattta cttcaaggt ttttcgaaa aacaaacaa 
      aaacggttt acggttaat acattttat ttaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY