| General information | |||
| TC010534 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 14738920..14785613 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010534 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04865 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC010534 RA
MLDVPRPTS RVEIVAAMR RIRYEFKAK GIKKKKVTV EVSVDGVKV TLRKKKKRK HWLDDGKPL LLSHPIYRI FYVSHDSQD LKIFSYIAR DGASNVFKC AVFKSNKKV YICEAERRD ATCQLHPNQ FVSGRPPEA SSPLCPNKM KLLRAFTFF KIMENSSSD HLTRLKMSG KDDLEMPDP EVVVKKKQD MLTKARNKL FNLGENLKT HKIDGDHHR KTVTQATID YIFKHCPKE KNHVYANSN IHTKRNLQK QNSVGAYPI KIEITQDSD EEKETIKRS RPATVCVDK VSEGRETAA LALERPRKK LSFREPEIV GRTKQEQQK THLEVVGLK RSSSYCNGI LHRSPSFED SDLESQAMR VVRTIGQAF EVCHKLSIN APENENLDQ DEQDTLTQD LLSDRLSDV TSDKPKKDL LSEGASDKM SLPPDDSSF RDYNETKSA KPQQLDILP PPPTTNTRK SPLTSAETY SSPHSDVLT SGSGSGGSN SLPPPGSAL SAHHELQLL REQLEQQSQ QTQAAMAQL QLAREQLAA EQSARLEAQ ARTHQLLIH NRELLDHIA ALVAHLQGG EKASQQQSP PHVTMPQQQ QQPGSGENY SSDHLSETA SLDNSSALQ ALGLNPQGL IENRAVTSC LPPSPLRTS YNPPGSVFN FSFPQVDAC YDPQLLQRL QNMGGYTPS YAYNYTQTL PYLPNLYNQ PLINNNYTL QSPTQKKLP SSPMPMRHS FAGSSPASE RRVSPARGG EPMYPGNHL QVQQAVQRQ PSPDRRDGQ FIKPLSQMG TLTATDPEG RVRVIVPVP ANSAEDTGT LMANLRLSD SLRVSDDRT LNGPPITRS TSEKVPNRS ELMSQVQRT MWARHTTK
- >TC010534 RA
atgttggac gtgcctcga ccgaccagc agggtggag atcgtggcg gccatgcga aggatacga tacgaattc aaggcaaag ggaattaag aagaagaag gtgacggtg gaggtctcg gtggatggt gttaaagtt acgctcagg aaaaagaag aagaggaaa cactggctg gacgatgga aagccgcta ctcctgtca catcccatc tatagaata ttttacgtg tcacacgat tctcaggac ttgaaaata tttagttat atagctagg gacggagct tccaatgtt tttaaatgt gcagtattc aaatcgaac aaaaaggtg tatatttgc gaggccgag cgtcgcgac gccacttgc caactccat cctaatcag ttcgttagc ggccggcca cctgaagca tcgagtccg ttgtgtccg aataaaatg aagctttta cgcgccttc acgttcttc aaaataatg gagaactcg tcgagcgac catctaaca cgcctcaag atgtctgga aaggatgac ctggaaatg ccggatccg gaagttgtc gtcaagaag aagcaagac atgctgacc aaagcacgg aacaagttg ttcaatttg ggtgagaac ttgaaaact cacaaaata gacggggac caccacagg aagacggtg actcaagcc accatcgac tacatcttc aaacattgc cccaaagag aagaaccac gtttatgcc aacagtaac atccacacc aagaggaat ctgcagaag cagaactcg gttggagct tatccgatc aaaatcgag atcactcaa gactccgat gaggaaaag gagactatc aaaagatca agaccggcc acggtttgt gttgacaaa gtgagtgaa gggcgggaa accgcagcg ttggccctg gaaaggccc cggaagaag ctttcgttc cgagagccg gaaatcgtg ggtcgcacc aagcaagag cagcagaag acccacttg gaggtggtg ggactgaag aggagttcg agctattgc aatggaatc ctgcaccga agccccagt ttcgaagac tctgacttg gagagccag gcaatgcgc gtggtcagg accataggt caagcgttc gaagtgtgc cataaactg tcgattaac gctcctgag aacgaaaac ttggatcag gacgaacaa gatacgctt actcaagac ttgctcagc gaccggtta agtgacgta acgagtgat aagccgaag aaagatttg ctctctgaa ggtgccagt gacaagatg tctcttcct cccgacgat tcgagtttc cgggactac aacgagacc aaatcggcc aaacctcaa cagctcgac attctcccg cctccacct accaccaac acccgaaaa tcacccttg acgtcagcg gaaacgtac tcttcgccg cacagcgac gtcctcacc agcgggagc ggcagcggc ggcagcaac tcgctgccg cctccagga agcgccctt tcggcgcat cacgagctg cagttgctc agagagcaa ttggaacag cagagtcag caaacgcaa gccgcaatg gcacaactg caactcgcg agggagcaa ctcgccgct gaacagagc gctcgtttg gaggcccaa gcccgaaca caccagctg ctcatccac aacagggaa ttgctcgat cacatagcc gccctcgtg gcgcacctc caagggggc gagaaagcc agtcaacag cagagccct ccgcacgtg acgatgccc cagcagcaa cagcagccg gggtcaggc gaaaactac tcctcggac cacctctcc gaaaccgct tcactggac aacagtagc gctttacaa gccttgggc ttaaacccc caaggcctg atcgaaaac cgagcagta acatcgtgt ctaccacct tcgcccctc cggacatcc tacaaccca cccggcagc gtgttcaac ttttccttc ccccaagtt gacgcctgc tacgacccc cagctcctc caaagactc cagaacatg ggcggctac accccttca tacgcctac aactacacc cagaccctc ccctacttg cccaacctg tacaaccaa cccctcatc aacaacaac tacaccctg cagtcgccg actcagaag aagctgccg tcgtcaccc atgcccatg aggcactcc ttcgccggg agcagcccc gcgtcggag cgccgggtg agccccgcc agagggggc gagcccatg tacccgggg aaccacctg caggtgcag caggcggtg cagagacag ccgagtccc gacagaagg gatggccag ttcatcaag cccctgtcc cagatgggc accttgacg gccactgat cccgaggga agggtgcgg gtgatagtt ccagtcccg gcgaactcg gcggaagac accgggacg ctgatggcc aacttgaga ctgagtgac agtttgagg gttagtgac gatcggact ctgaacgga ccgccgatc acacggtcg acgagtgag aaagtgccc aacaggagc gaactgatg tcgcaagtg cagcgcacc atgtgggca agacacacc accaagtga
- >TC010534 RA
ttattttta tgtttattg aaattgtgt ttttatttt cagtatatc gggatgttg gacgtgcct cgaccgacc agcagggtg gagatcgtg gcggccatg cgaaggata cgatacgaa ttcaaggca aagggaatt aagaagaag aaggtgacg gtggaggtc tcggtggat ggtgttaaa gttacgctc aggaaaaag aagaagagg aaacactgg ctggacgat ggaaagccg ctactcctg tcacatccc atctataga atattttac gtgtcacac gattctcag gacttgaaa atatttagt tatatagct agggacgga gcttccaat gtttttaaa tgtgcagta ttcaaatcg aacaaaaag gtgtatatt tgcgaggcc gagcgtcgc gacgccact tgccaactc catcctaat cagttcgtt agcggccgg ccacctgaa gcatcgagt ccgttgtgt ccgaataaa atgaagctt ttacgcgcc ttcacgttc ttcaaaata atggagaac tcgtcgagc gaccatcta acacgcctc aagatgtct ggaaaggat gacctggaa atgccggat ccggaagtt gtcgtcaag aagaagcaa gacatgctg accaaagca cggaacaag ttgttcaat ttgggtgag aacttgaaa actcacaaa atagacggg gaccaccac aggaagacg gtgactcaa gccaccatc gactacatc ttcaaacat tgccccaaa gagaagaac cacgtttat gccaacagt aacatccac accaagagg aatctgcag aagcagaac tcggttgga gcttatccg atcaaaatc gagatcact caagactcc gatgaggaa aaggagact atcaaaaga tcaagaccg gccacggtt tgtgttgac aaagtgagt gaagggcgg gaaaccgca gcgttggcc ctggaaagg ccccggaag aagctttcg ttccgagag ccggaaatc gtgggtcgc accaagcaa gagcagcag aagacccac ttggaggtg gtgggactg aagaggagt tcgagctat tgcaatgga atcctgcac cgaagcccc agtttcgaa gactctgac ttggagagc caggcaatg cgcgtggtc aggaccata ggtcaagcg ttcgaagtg tgccataaa ctgtcgatt aacgctcct gagaacgaa aacttggat caggacgaa caagatacg cttactcaa gacttgctc agcgaccgg ttaagtgac gtaacgagt gataagccg aagaaagat ttgctctct gaaggtgcc agtgacaag atgtctctt cctcccgac gattcgagt ttccgggac tacaacgag accaaatcg gccaaacct caacagctc gacattctc ccgcctcca cctaccacc aacacccga aaatcaccc ttgacgtca gcggaaacg tactcttcg ccgcacagc gacgtcctc accagcggg agcggcagc ggcggcagc aactcgctg ccgcctcca ggaagcgcc ctttcggcg catcacgag ctgcagttg ctcagagag caattggaa cagcagagt cagcaaacg caagccgca atggcacaa ctgcaactc gcgagggag caactcgcc gctgaacag agcgctcgt ttggaggcc caagcccga acacaccag ctgctcatc cacaacagg gaattgctc gatcacata gccgccctc gtggcgcac ctccaaggg ggcgagaaa gccagtcaa cagcagagc cctccgcac gtgacgatg ccccagcag caacagcag ccggggtca ggcgaaaac tactcctcg gaccacctc tccgaaacc gcttcactg gacaacagt agcgcttta caagccttg ggcttaaac ccccaaggc ctgatcgaa aaccgagca gtaacatcg tgtctacca ccttcgccc ctccggaca tcctacaac ccacccggc agcgtgttc aacttttcc ttcccccaa gttgacgcc tgctacgac ccccagctc ctccaaaga ctccagaac atgggcggc tacacccct tcatacgcc tacaactac acccagacc ctcccctac ttgcccaac ctgtacaac caacccctc atcaacaac aactacacc ctgcagtcg ccgactcag aagaagctg ccgtcgtca cccatgccc atgaggcac tccttcgcc gggagcagc cccgcgtcg gagcgccgg gtgagcccc gccagaggg ggcgagccc atgtacccg gggaaccac ctgcaggtg cagcaggcg gtgcagaga cagccgagt cccgacaga agggatggc cagttcatc aagcccctg tcccagatg ggcaccttg acggccact gatcccgag ggaagggtg cgggtgata gttccagtc ccggcgaac tcggcggaa gacaccggg acgctgatg gccaacttg agactgagt gacagtttg agggttagt gacgatcgg actctgaac ggaccgccg atcacacgg tcgacgagt gagaaagtg cccaacagg agcgaactg atgtcgcaa gtgcagcgc accatgtgg gcaagacac accaccaag tgatcatga catatcaga gttgcgatc gttaactta aggactaat tatagcatt ttgttttgt ttttcgtcg gtttttgtt tataaaaca cgtccagaa ctgctacca gatcgtagg aatacaagt aaatttagg cttgtttat attaggttc taatatcat ttacttgat aagcagttg tagaggtgt tagtaggaa aaatcagat cagtgttcg atatttttt ctagtcaaa ctatattta cttcaaggt ttttcgaaa aacaaacaa aaacggttt acggttaat acattttat ttaaaaat
