| General information | |||
| TC010526 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 14437824..14446059 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010526 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04862 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC010526 RA
MSISQDDIS ELNSPIKPV PRKSKNRNK VTSPVEQSP LSNPSKKDV FEMFTIKTP SIQGRAKVL TSDSQDTSE LSDEVFTKP KPVSLSTPS KSYREKINA RFKNVKEKA GKSTKKANV FKEAAENIR LEKVKRESH DIDAELELM RERLKLGKP PDNKHIFEF FSSEKCHET EEKESEDNF QSTDDLTDD GPALVFSLP KQIKTLLRK SEDALFYTP QAEPVDITD KIETEEPRF LEDEGFYVT SSPKIPNKH LYLLEQRLS CDDKKWFNC DGKLNILEN PCQRSSYRP RLKHLPVKE IATYLPATT ESNECEEIV VVSENLLQL RVLSLKFTH HPLFSLEHV LCQKLKKLY YEYEESVTK NEFMRLCGR LEGLRRVLR RLEENSSQS GDQSDREDG KVEAYKRDI RTLQEQVFE EGKKERDRI IEILQTWKA IKSMRENNK YSNTSLKLI IKKENVNYE TEKENFDKN FEETYEEFL QINREKPRR KRQTEEDIR QELVERFKE SFRPPGEPI LHFILTNEN EITKQVENS KEVSRRTAT TTTKIALKI LCNKMEVCK TKWLALQDD FVCNFNETI SIQLTNVPK FLTLEIIEQ PKSLIKRTL GELTVKIPA RSVSKIDKK NAKFEKNEI VHYKHEGVG SGIKVDSLV QNLGDFELN TSGCLTYSL SWDASVAND NSPDNRAQI LGEIIDKSG TIDSEKLVQ WMQQHNPDP QDPRNSILY EYSLDCGEE MVSASSKNY FRLNEQEWQ FCDPKIIED NLRFKILQL RNKNEPEFD RIVVPNRIK EIPLDVLAD YRRRVAMER ESLVEEDED DDDEIEARR SKGRKLLKQ IHIRVFQKC KNSENNLSF EDVVDEKFM PQLEKLIKG FGANFLSWF QWRPLKPPL FPLKTLQES KSNDYDLQS RVKINVHVI SGLNIPLRE GKAMPFVEI QYGNATVRT VASQGAHPN WNEKLVLPL EPSHLDYLN PNSLSGSLI LNVFDESEV VLMKFAGQK GRTWLGYVE IPLSAVCIG MQGMFRVKV PHMLLGYQG KTLETTRSN SQSNQQTYL NLHVTMEPN VPKLNPAID ELPCADVPY IQDYVMKWN TDYNTTFPN RKFSVLAID ANATTSCII RYIKPLEPP QINVEGFDV TPEQCARFV SLIPFTDCN QFYKNIWLS VDRFLHYMT GSIIDHAVA LTCYLLALK LEVWLLLGF GIPHGATAY VLVKEYDNN RVANFYVYD VVSGNKIYL TDAFCPLQR VYCLVNEHN VWANIQRND DVTVMRFDL TRRSDWLPL FTSQISAPT NSIHGKITY LSHTNTDEL EIILDRKIR KRFAKLRQL DRTLWNHDV SNTFKSVLG SFEQAEMYG KSNSDALEK IKECLAGFE MRGQIFNLP FTNCAAIVK HVKTFGLHL EEDANVQFA CAVYLRAYP NQVLTVWVA LAVIKNKKT DDPLQEVL
- >TC010526 RA
atgtcaatt tcgcaggat gacatcagc gaactgaat tcccctata aaaccagta cctagaaaa tccaaaaat cggaacaag gtaacttct ccagtggag caatcgccc ctttccaat ccttcaaag aaagatgtt ttcgaaatg ttcacaatc aaaacgccc tcaatccag ggaagggcc aaagttttg acctccgac agccaagac acatccgaa ttatccgac gaagtcttc accaagccg aaaccagtc tcactttca actcctagt aaatcatac cgagaaaaa attaacgca aggttcaaa aatgtaaag gaaaaagct gggaaatcc acaaagaag gcaaacgtt ttcaaggaa gctgcagaa aatattcgg ttagaaaaa gtgaagagg gaaagtcac gacatcgac gccgagctt gaattaatg agagagcgt cttaaactg ggaaaacct ccagataac aagcacatt ttcgagttt ttttctagt gaaaaatgc cacgaaact gaagagaaa gaaagtgaa gacaatttt caatcaact gacgatctg accgacgac ggacccgca ctagttttt tcattacct aagcaaata aaaacgctt ctaaggaaa tcagaagac gcgttattt tacactcct caggcagaa cctgttgac atcactgat aaaatcgag actgaagaa cccagattt ttggaagat gaaggcttt tatgtcact tccagtccc aaaatacct aacaagcat ttatatctt ttggagcag cgtttgtca tgtgacgat aaaaaatgg ttcaactgt gatggaaaa ctaaacatt ttggaaaat ccctgtcaa agaagctca taccgtcca cgtttgaag catcttcca gtgaaagaa attgccact tacttaccg gcaacaacc gaaagtaac gaatgtgaa gaaattgtg gttgttagt gagaatttg ctccaacta cgagtttta agtctgaaa tttactcat cacccctta ttcagttta gaacatgtg ctttgccaa aaactgaag aagttgtat tacgagtat gaagaaagt gtcaccaaa aatgagttt atgagactt tgtggtaga ttagaaggt ttgagacgt gttttgaga cgtttggaa gaaaattca agtcaatca ggcgatcag tcagataga gaagatggc aaagtggaa gcttacaag cgcgacata cgaacactg caagaacaa gttttcgaa gaaggaaaa aaagagaga gaccgtatc attgaaatt ttgcaaacg tggaaagct attaaaagc atgagagaa aataacaag tactcgaac acctctctg aaactgata ataaagaag gagaatgtc aattacgag acggagaaa gagaatttt gacaaaaac tttgaagaa acctacgaa gagtttctc caaataaat cgggaaaaa cctcgccgg aagagacaa actgaagaa gacatcaga caagaactg gtcgaacgt ttcaaggaa agttttagg cctcctggt gagcccatc ctacatttt atcttaaca aacgaaaat gaaataacc aaacaagtg gaaaattcc aaagaagtg tcgcgaaga accgctact acaaccacc aaaatcgct ctcaaaata ctttgcaac aagatggag gtgtgtaag acaaaatgg ctggcgctc caagacgat ttcgtttgc aacttcaac gaaaccata tcgattcaa ctaaccaac gttccgaaa ttcctcaca cttgagata atcgagcaa ccaaagtca cttattaaa aggaccctc ggagagttg acggttaaa ataccagct cgaagtgtt tcaaaaatc gataaaaaa aacgccaaa tttgagaaa aacgaaatc gttcattac aagcatgag ggggtggga agcggcatt aaagtcgac tctcttgtg caaaatttg ggcgatttt gaactgaac acttcgggt tgtttgacc tattcattg tcctgggat gcgtccgtg gcgaatgat aacagtcca gataacagg gcacaaatt ttgggtgag ataatagac aagagtgga acaatagat tcggaaaaa ctggtccag tggatgcag cagcacaac cctgacccc caagatcca agaaattcg atcttgtac gaatattcg cttgactgt ggcgaggaa atggtttct gcaagtagt aaaaactat ttccggttg aacgaacaa gagtggcaa ttttgcgac ccgaaaata attgaggat aatctaagg ttcaaaatc ttgcaactg cggaacaag aatgaaccg gaatttgac cgaatagta gtcccaaac cggatcaaa gaaattcct cttgatgtc ttagccgat tatagaagg agggtggcg atggaacgg gagagtttg gttgaagag gatgaagac gatgacgac gaaattgag gcaagaagg agcaaaggg cggaagttg ttgaagcaa atccacatc cgagtgttc caaaaatgc aaaaactcc gaaaataat ctcagtttt gaagacgtg gtagatgag aaattcatg cctcagctg gagaaactg atcaaaggc tttggtgcc aatttcctt agttggttt cagtggcgg cccttgaaa ccgccgttg tttccctta aaaacgctt caagaatcg aaatcaaac gattacgat ttgcaaagt cgggtcaag attaacgtt cacgtaatt tcgggttta aacattcct ctacgagag ggcaaagca atgcctttt gttgagatt cagtacggc aatgctaca gttcgaact gttgcaagt cagggagct cacccgaac tggaacgag aaattggtt ttgccactt gaaccgtct catttggat tatttgaat ccaaattcg cttagtggt agtttgatt ttgaacgtt ttcgatgaa agtgaagta gttttgatg aaatttgcg gggcagaaa ggccgcaca tggctaggg tatgtggaa ataccctta tcagccgtt tgtattggc atgcaaggc atgtttagg gtcaaggtg cctcacatg ttgctggga tatcaaggc aaaactttg gagacaaca agaagtaac tctcaaagt aatcaacaa acttacttg aatctccac gttactatg gaacctaat gttcctaaa cttaatcct gcaatagac gagcttcct tgtgccgac gttccttac attcaggac tacgttatg aaatggaac acggattac aacaccact tttccaaac cgtaaattt tcagttttg gcaattgat gcaaacgcg acaacttca tgtatcatc cgctacatc aagcctctg gaacctccc caaataaac gtggaagga tttgatgtg acacctgaa cagtgtgcc cgatttgtc tccctgatt cccttcacc gattgcaac cagttttac aaaaatatt tggctttca gttgatcga tttttgcat tacatgact ggctcaatc attgaccac gctgtagca ctgacatgt tatctcctc gcgttaaaa ctggaggtt tggcttttg ctaggtttt ggtattcct catggagcc actgcttat gtccttgtg aaagaatat gacaacaat cgggtcgca aacttttac gtttatgac gtcgtttca ggcaataaa atctacttg actgatgct ttttgcccc cttcaacga gtttattgc ctcgttaat gaacacaac gtgtgggcc aacattcaa agaaatgac gatgtgaca gtgatgaga tttgattta acgcgacgt tctgattgg ttacctctt tttaccagt caaatatca gctccaacc aattcaatt catggaaaa attacgtac ttgtctcac acaaataca gacgagttg gaaattatt cttgacagg aaaattcgg aagcgtttc gccaagctg aggcaactt gatcgaact ctttggaac catgacgtt tcaaatact tttaaaagt gttttgggt tctttcgaa caggcggag atgtacggt aaaagtaat tcagatgct ttagaaaaa attaaggag tgtttggct ggttttgag atgagagga cagattttc aatttaccg ttcaccaac tgtgcagcg attgtaaaa cacgtcaaa acgtttgga ttgcatttg gaggaagat gctaacgta cagtttgca tgtgctgtg tatctgagg gcttatccg aatcaagtt ttgactgtt tgggtcgct ctagctgtg attaagaat aaaaaaact gacgatcca ttacaagag gtattataa
- >TC010526 RA
cagtcacaa caatgtcaa tttcgcagg atgacatca gcgaactga attccccta taaaaccag tacctagaa aatccaaaa atcggaaca aggtaactt ctccagtgg agcaatcgc ccctttcca atccttcaa agaaagatg ttttcgaaa tgttcacaa tcaaaacgc cctcaatcc agggaaggg ccaaagttt tgacctccg acagccaag acacatccg aattatccg acgaagtct tcaccaagc cgaaaccag tctcacttt caactccta gtaaatcat accgagaaa aaattaacg caaggttca aaaatgtaa aggaaaaag ctgggaaat ccacaaaga aggcaaacg ttttcaagg aagctgcag aaaatattc ggttagaaa aagtgaaga gggaaagtc acgacatcg acgccgagc ttgaattaa tgagagagc gtcttaaac tgggaaaac ctccagata acaagcaca ttttcgagt ttttttcta gtgaaaaat gccacgaaa ctgaagaga aagaaagtg aagacaatt ttcaatcaa ctgacgatc tgaccgacg acggacccg cactagttt tttcattac ctaagcaaa taaaaacgc ttctaagga aatcagaag acgcgttat tttacactc ctcaggcag aacctgttg acatcactg ataaaatcg agactgaag aacccagat ttttggaag atgaaggct tttatgtca cttccagtc ccaaaatac ctaacaagc atttatatc ttttggagc agcgtttgt catgtgacg ataaaaaat ggttcaact gtgatggaa aactaaaca ttttggaaa atccctgtc aaagaagct cataccgtc cacgtttga agcatcttc cagtgaaag aaattgcca cttacttac cggcaacaa ccgaaagta acgaatgtg aagaaattg tggttgtta gtgagaatt tgctccaac tacgagttt taagtctga aatttactc atcacccct tattcagtt tagaacatg tgctttgcc aaaaactga agaagttgt attacgagt atgaagaaa gtgtcacca aaaatgagt ttatgagac tttgtggta gattagaag gtttgagac gtgttttga gacgtttgg aagaaaatt caagtcaat caggcgatc agtcagata gagaagatg gcaaagtgg aagcttaca agcgcgaca tacgaacac tgcaagaac aagttttcg aagaaggaa aaaaagaga gagaccgta tcattgaaa ttttgcaaa cgtggaaag ctattaaaa gcatgagag aaaataaca agtactcga acacctctc tgaaactga taataaaga aggagaatg tcaattacg agacggaga aagagaatt ttgacaaaa actttgaag aaacctacg aagagtttc tccaaataa atcgggaaa aacctcgcc ggaagagac aaactgaag aagacatca gacaagaac tggtcgaac gtttcaagg aaagtttta ggcctcctg gtgagccca tcctacatt ttatcttaa caaacgaaa atgaaataa ccaaacaag tggaaaatt ccaaagaag tgtcgcgaa gaaccgcta ctacaacca ccaaaatcg ctctcaaaa tactttgca acaagatgg aggtgtgta agacaaaat ggctggcgc tccaagacg atttcgttt gcaacttca acgaaacca tatcgattc aactaacca acgttccga aattcctca cacttgaga taatcgagc aaccaaagt cacttatta aaaggaccc tcggagagt tgacggtta aaataccag ctcgaagtg tttcaaaaa tcgataaaa aaaacgcca aatttgaga aaaacgaaa tcgttcatt acaagcatg agggggtgg gaagcggca ttaaagtcg actctcttg tgcaaaatt tgggcgatt ttgaactga acacttcgg gttgtttga cctattcat tgtcctggg atgcgtccg tggcgaatg ataacagtc cagataaca gggcacaaa ttttgggtg agataatag acaagagtg gaacaatag attcggaaa aactggtcc agtggatgc agcagcaca accctgacc cccaagatc caagaaatt cgatcttgt acgaatatt cgcttgact gtggcgagg aaatggttt ctgcaagta gtaaaaact atttccggt tgaacgaac aagagtggc aattttgcg acccgaaaa taattgagg ataatctaa ggttcaaaa tcttgcaac tgcggaaca agaatgaac cggaatttg accgaatag tagtcccaa accggatca aagaaattc ctcttgatg tcttagccg attatagaa ggagggtgg cgatggaac gggagagtt tggttgaag aggatgaag acgatgacg acgaaattg aggcaagaa ggagcaaag ggcggaagt tgttgaagc aaatccaca tccgagtgt tccaaaaat gcaaaaact ccgaaaata atctcagtt ttgaagacg tggtagatg agaaattca tgcctcagc tggagaaac tgatcaaag gctttggtg ccaatttcc ttagttggt ttcagtggc ggcccttga aaccgccgt tgtttccct taaaaacgc ttcaagaat cgaaatcaa acgattacg atttgcaaa gtcgggtca agattaacg ttcacgtaa tttcgggtt taaacattc ctctacgag agggcaaag caatgcctt ttgttgaga ttcagtacg gcaatgcta cagttcgaa ctgttgcaa gtcagggag ctcacccga actggaacg agaaattgg ttttgccac ttgaaccgt ctcatttgg attatttga atccaaatt cgcttagtg gtagtttga ttttgaacg ttttcgatg aaagtgaag tagttttga tgaaatttg cggggcaga aaggccgca catggctag ggtatgtgg aaataccct tatcagccg tttgtattg gcatgcaag gcatgttta gggtcaagg tgcctcaca tgttgctgg gatatcaag gcaaaactt tggagacaa caagaagta actctcaaa gtaatcaac aaacttact tgaatctcc acgttacta tggaaccta atgttccta aacttaatc ctgcaatag acgagcttc cttgtgccg acgttcctt acattcagg actacgtta tgaaatgga acacggatt acaacacca cttttccaa accgtaaat tttcagttt tggcaattg atgcaaacg cgacaactt catgtatca tccgctaca tcaagcctc tggaacctc cccaaataa acgtggaag gatttgatg tgacacctg aacagtgtg cccgatttg tctccctga ttcccttca ccgattgca accagtttt acaaaaata tttggcttt cagttgatc gatttttgc attacatga ctggctcaa tcattgacc acgctgtag cactgacat gttatctcc tcgcgttaa aactggagg tttggcttt tgctaggtt ttggtattc ctcatggag ccactgctt atgtccttg tgaaagaat atgacaaca atcgggtcg caaactttt acgtttatg acgtcgttt caggcaata aaatctact tgactgatg ctttttgcc cccttcaac gagtttatt gcctcgtta atgaacaca acgtgtggg ccaacattc aaagaaatg acgatgtga cagtgatga gatttgatt taacgcgac gttctgatt ggttacctc tttttacca gtcaaatat cagctccaa ccaattcaa ttcatggaa aaattacgt acttgtctc acacaaata cagacgagt tggaaatta ttcttgaca ggaaaattc ggaagcgtt tcgccaagc tgaggcaac ttgatcgaa ctctttgga accatgacg tttcaaata cttttaaaa gtgttttgg gttctttcg aacaggcgg agatgtacg gtaaaagta attcagatg ctttagaaa aaattaagg agtgtttgg ctggttttg agatgagag gacagattt tcaatttac cgttcacca actgtgcag cgattgtaa aacacgtca aaacgtttg gattgcatt tggaggaag atgctaacg tacagtttg catgtgctg tgtatctga gggcttatc cgaatcaag ttttgactg tttgggtcg ctctagctg tgattaaga ataaaaaaa ctgacgatc cattacaag aggtattat aattttatc ctttgtaag gttcgaaca ataaaatga agctttttt aaccaaatt gtatcattc aaacaaata tcagatgaa taaaaagta tgaaaccaa
