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Gene information
General information
TC010526 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14437824..14446059
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010526
    iBeetle screen iB_04862

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010526 RA
      MSISQDDIS ELNSPIKPV PRKSKNRNK VTSPVEQSP 
      LSNPSKKDV FEMFTIKTP SIQGRAKVL TSDSQDTSE 
      LSDEVFTKP KPVSLSTPS KSYREKINA RFKNVKEKA 
      GKSTKKANV FKEAAENIR LEKVKRESH DIDAELELM 
      RERLKLGKP PDNKHIFEF FSSEKCHET EEKESEDNF 
      QSTDDLTDD GPALVFSLP KQIKTLLRK SEDALFYTP 
      QAEPVDITD KIETEEPRF LEDEGFYVT SSPKIPNKH 
      LYLLEQRLS CDDKKWFNC DGKLNILEN PCQRSSYRP 
      RLKHLPVKE IATYLPATT ESNECEEIV VVSENLLQL 
      RVLSLKFTH HPLFSLEHV LCQKLKKLY YEYEESVTK 
      NEFMRLCGR LEGLRRVLR RLEENSSQS GDQSDREDG 
      KVEAYKRDI RTLQEQVFE EGKKERDRI IEILQTWKA 
      IKSMRENNK YSNTSLKLI IKKENVNYE TEKENFDKN 
      FEETYEEFL QINREKPRR KRQTEEDIR QELVERFKE 
      SFRPPGEPI LHFILTNEN EITKQVENS KEVSRRTAT 
      TTTKIALKI LCNKMEVCK TKWLALQDD FVCNFNETI 
      SIQLTNVPK FLTLEIIEQ PKSLIKRTL GELTVKIPA 
      RSVSKIDKK NAKFEKNEI VHYKHEGVG SGIKVDSLV 
      QNLGDFELN TSGCLTYSL SWDASVAND NSPDNRAQI 
      LGEIIDKSG TIDSEKLVQ WMQQHNPDP QDPRNSILY 
      EYSLDCGEE MVSASSKNY FRLNEQEWQ FCDPKIIED 
      NLRFKILQL RNKNEPEFD RIVVPNRIK EIPLDVLAD 
      YRRRVAMER ESLVEEDED DDDEIEARR SKGRKLLKQ 
      IHIRVFQKC KNSENNLSF EDVVDEKFM PQLEKLIKG 
      FGANFLSWF QWRPLKPPL FPLKTLQES KSNDYDLQS 
      RVKINVHVI SGLNIPLRE GKAMPFVEI QYGNATVRT 
      VASQGAHPN WNEKLVLPL EPSHLDYLN PNSLSGSLI 
      LNVFDESEV VLMKFAGQK GRTWLGYVE IPLSAVCIG 
      MQGMFRVKV PHMLLGYQG KTLETTRSN SQSNQQTYL 
      NLHVTMEPN VPKLNPAID ELPCADVPY IQDYVMKWN 
      TDYNTTFPN RKFSVLAID ANATTSCII RYIKPLEPP 
      QINVEGFDV TPEQCARFV SLIPFTDCN QFYKNIWLS 
      VDRFLHYMT GSIIDHAVA LTCYLLALK LEVWLLLGF 
      GIPHGATAY VLVKEYDNN RVANFYVYD VVSGNKIYL 
      TDAFCPLQR VYCLVNEHN VWANIQRND DVTVMRFDL 
      TRRSDWLPL FTSQISAPT NSIHGKITY LSHTNTDEL 
      EIILDRKIR KRFAKLRQL DRTLWNHDV SNTFKSVLG 
      SFEQAEMYG KSNSDALEK IKECLAGFE MRGQIFNLP 
      FTNCAAIVK HVKTFGLHL EEDANVQFA CAVYLRAYP 
      NQVLTVWVA LAVIKNKKT DDPLQEVL
    CDS sequence
    • >TC010526 RA
      atgtcaatt tcgcaggat gacatcagc gaactgaat 
      tcccctata aaaccagta cctagaaaa tccaaaaat 
      cggaacaag gtaacttct ccagtggag caatcgccc 
      ctttccaat ccttcaaag aaagatgtt ttcgaaatg 
      ttcacaatc aaaacgccc tcaatccag ggaagggcc 
      aaagttttg acctccgac agccaagac acatccgaa 
      ttatccgac gaagtcttc accaagccg aaaccagtc 
      tcactttca actcctagt aaatcatac cgagaaaaa 
      attaacgca aggttcaaa aatgtaaag gaaaaagct 
      gggaaatcc acaaagaag gcaaacgtt ttcaaggaa 
      gctgcagaa aatattcgg ttagaaaaa gtgaagagg 
      gaaagtcac gacatcgac gccgagctt gaattaatg 
      agagagcgt cttaaactg ggaaaacct ccagataac 
      aagcacatt ttcgagttt ttttctagt gaaaaatgc 
      cacgaaact gaagagaaa gaaagtgaa gacaatttt 
      caatcaact gacgatctg accgacgac ggacccgca 
      ctagttttt tcattacct aagcaaata aaaacgctt 
      ctaaggaaa tcagaagac gcgttattt tacactcct 
      caggcagaa cctgttgac atcactgat aaaatcgag 
      actgaagaa cccagattt ttggaagat gaaggcttt 
      tatgtcact tccagtccc aaaatacct aacaagcat 
      ttatatctt ttggagcag cgtttgtca tgtgacgat 
      aaaaaatgg ttcaactgt gatggaaaa ctaaacatt 
      ttggaaaat ccctgtcaa agaagctca taccgtcca 
      cgtttgaag catcttcca gtgaaagaa attgccact 
      tacttaccg gcaacaacc gaaagtaac gaatgtgaa 
      gaaattgtg gttgttagt gagaatttg ctccaacta 
      cgagtttta agtctgaaa tttactcat cacccctta 
      ttcagttta gaacatgtg ctttgccaa aaactgaag 
      aagttgtat tacgagtat gaagaaagt gtcaccaaa 
      aatgagttt atgagactt tgtggtaga ttagaaggt 
      ttgagacgt gttttgaga cgtttggaa gaaaattca 
      agtcaatca ggcgatcag tcagataga gaagatggc 
      aaagtggaa gcttacaag cgcgacata cgaacactg 
      caagaacaa gttttcgaa gaaggaaaa aaagagaga 
      gaccgtatc attgaaatt ttgcaaacg tggaaagct 
      attaaaagc atgagagaa aataacaag tactcgaac 
      acctctctg aaactgata ataaagaag gagaatgtc 
      aattacgag acggagaaa gagaatttt gacaaaaac 
      tttgaagaa acctacgaa gagtttctc caaataaat 
      cgggaaaaa cctcgccgg aagagacaa actgaagaa 
      gacatcaga caagaactg gtcgaacgt ttcaaggaa 
      agttttagg cctcctggt gagcccatc ctacatttt 
      atcttaaca aacgaaaat gaaataacc aaacaagtg 
      gaaaattcc aaagaagtg tcgcgaaga accgctact 
      acaaccacc aaaatcgct ctcaaaata ctttgcaac 
      aagatggag gtgtgtaag acaaaatgg ctggcgctc 
      caagacgat ttcgtttgc aacttcaac gaaaccata 
      tcgattcaa ctaaccaac gttccgaaa ttcctcaca 
      cttgagata atcgagcaa ccaaagtca cttattaaa 
      aggaccctc ggagagttg acggttaaa ataccagct 
      cgaagtgtt tcaaaaatc gataaaaaa aacgccaaa 
      tttgagaaa aacgaaatc gttcattac aagcatgag 
      ggggtggga agcggcatt aaagtcgac tctcttgtg 
      caaaatttg ggcgatttt gaactgaac acttcgggt 
      tgtttgacc tattcattg tcctgggat gcgtccgtg 
      gcgaatgat aacagtcca gataacagg gcacaaatt 
      ttgggtgag ataatagac aagagtgga acaatagat 
      tcggaaaaa ctggtccag tggatgcag cagcacaac 
      cctgacccc caagatcca agaaattcg atcttgtac 
      gaatattcg cttgactgt ggcgaggaa atggtttct 
      gcaagtagt aaaaactat ttccggttg aacgaacaa 
      gagtggcaa ttttgcgac ccgaaaata attgaggat 
      aatctaagg ttcaaaatc ttgcaactg cggaacaag 
      aatgaaccg gaatttgac cgaatagta gtcccaaac 
      cggatcaaa gaaattcct cttgatgtc ttagccgat 
      tatagaagg agggtggcg atggaacgg gagagtttg 
      gttgaagag gatgaagac gatgacgac gaaattgag 
      gcaagaagg agcaaaggg cggaagttg ttgaagcaa 
      atccacatc cgagtgttc caaaaatgc aaaaactcc 
      gaaaataat ctcagtttt gaagacgtg gtagatgag 
      aaattcatg cctcagctg gagaaactg atcaaaggc 
      tttggtgcc aatttcctt agttggttt cagtggcgg 
      cccttgaaa ccgccgttg tttccctta aaaacgctt 
      caagaatcg aaatcaaac gattacgat ttgcaaagt 
      cgggtcaag attaacgtt cacgtaatt tcgggttta 
      aacattcct ctacgagag ggcaaagca atgcctttt 
      gttgagatt cagtacggc aatgctaca gttcgaact 
      gttgcaagt cagggagct cacccgaac tggaacgag 
      aaattggtt ttgccactt gaaccgtct catttggat 
      tatttgaat ccaaattcg cttagtggt agtttgatt 
      ttgaacgtt ttcgatgaa agtgaagta gttttgatg 
      aaatttgcg gggcagaaa ggccgcaca tggctaggg 
      tatgtggaa ataccctta tcagccgtt tgtattggc 
      atgcaaggc atgtttagg gtcaaggtg cctcacatg 
      ttgctggga tatcaaggc aaaactttg gagacaaca 
      agaagtaac tctcaaagt aatcaacaa acttacttg 
      aatctccac gttactatg gaacctaat gttcctaaa 
      cttaatcct gcaatagac gagcttcct tgtgccgac 
      gttccttac attcaggac tacgttatg aaatggaac 
      acggattac aacaccact tttccaaac cgtaaattt 
      tcagttttg gcaattgat gcaaacgcg acaacttca 
      tgtatcatc cgctacatc aagcctctg gaacctccc 
      caaataaac gtggaagga tttgatgtg acacctgaa 
      cagtgtgcc cgatttgtc tccctgatt cccttcacc 
      gattgcaac cagttttac aaaaatatt tggctttca 
      gttgatcga tttttgcat tacatgact ggctcaatc 
      attgaccac gctgtagca ctgacatgt tatctcctc 
      gcgttaaaa ctggaggtt tggcttttg ctaggtttt 
      ggtattcct catggagcc actgcttat gtccttgtg 
      aaagaatat gacaacaat cgggtcgca aacttttac 
      gtttatgac gtcgtttca ggcaataaa atctacttg 
      actgatgct ttttgcccc cttcaacga gtttattgc 
      ctcgttaat gaacacaac gtgtgggcc aacattcaa 
      agaaatgac gatgtgaca gtgatgaga tttgattta 
      acgcgacgt tctgattgg ttacctctt tttaccagt 
      caaatatca gctccaacc aattcaatt catggaaaa 
      attacgtac ttgtctcac acaaataca gacgagttg 
      gaaattatt cttgacagg aaaattcgg aagcgtttc 
      gccaagctg aggcaactt gatcgaact ctttggaac 
      catgacgtt tcaaatact tttaaaagt gttttgggt 
      tctttcgaa caggcggag atgtacggt aaaagtaat 
      tcagatgct ttagaaaaa attaaggag tgtttggct 
      ggttttgag atgagagga cagattttc aatttaccg 
      ttcaccaac tgtgcagcg attgtaaaa cacgtcaaa 
      acgtttgga ttgcatttg gaggaagat gctaacgta 
      cagtttgca tgtgctgtg tatctgagg gcttatccg 
      aatcaagtt ttgactgtt tgggtcgct ctagctgtg 
      attaagaat aaaaaaact gacgatcca ttacaagag 
      gtattataa
    mRNA sequence
    • >TC010526 RA
      cagtcacaa caatgtcaa tttcgcagg atgacatca 
      gcgaactga attccccta taaaaccag tacctagaa 
      aatccaaaa atcggaaca aggtaactt ctccagtgg 
      agcaatcgc ccctttcca atccttcaa agaaagatg 
      ttttcgaaa tgttcacaa tcaaaacgc cctcaatcc 
      agggaaggg ccaaagttt tgacctccg acagccaag 
      acacatccg aattatccg acgaagtct tcaccaagc 
      cgaaaccag tctcacttt caactccta gtaaatcat 
      accgagaaa aaattaacg caaggttca aaaatgtaa 
      aggaaaaag ctgggaaat ccacaaaga aggcaaacg 
      ttttcaagg aagctgcag aaaatattc ggttagaaa 
      aagtgaaga gggaaagtc acgacatcg acgccgagc 
      ttgaattaa tgagagagc gtcttaaac tgggaaaac 
      ctccagata acaagcaca ttttcgagt ttttttcta 
      gtgaaaaat gccacgaaa ctgaagaga aagaaagtg 
      aagacaatt ttcaatcaa ctgacgatc tgaccgacg 
      acggacccg cactagttt tttcattac ctaagcaaa 
      taaaaacgc ttctaagga aatcagaag acgcgttat 
      tttacactc ctcaggcag aacctgttg acatcactg 
      ataaaatcg agactgaag aacccagat ttttggaag 
      atgaaggct tttatgtca cttccagtc ccaaaatac 
      ctaacaagc atttatatc ttttggagc agcgtttgt 
      catgtgacg ataaaaaat ggttcaact gtgatggaa 
      aactaaaca ttttggaaa atccctgtc aaagaagct 
      cataccgtc cacgtttga agcatcttc cagtgaaag 
      aaattgcca cttacttac cggcaacaa ccgaaagta 
      acgaatgtg aagaaattg tggttgtta gtgagaatt 
      tgctccaac tacgagttt taagtctga aatttactc 
      atcacccct tattcagtt tagaacatg tgctttgcc 
      aaaaactga agaagttgt attacgagt atgaagaaa 
      gtgtcacca aaaatgagt ttatgagac tttgtggta 
      gattagaag gtttgagac gtgttttga gacgtttgg 
      aagaaaatt caagtcaat caggcgatc agtcagata 
      gagaagatg gcaaagtgg aagcttaca agcgcgaca 
      tacgaacac tgcaagaac aagttttcg aagaaggaa 
      aaaaagaga gagaccgta tcattgaaa ttttgcaaa 
      cgtggaaag ctattaaaa gcatgagag aaaataaca 
      agtactcga acacctctc tgaaactga taataaaga 
      aggagaatg tcaattacg agacggaga aagagaatt 
      ttgacaaaa actttgaag aaacctacg aagagtttc 
      tccaaataa atcgggaaa aacctcgcc ggaagagac 
      aaactgaag aagacatca gacaagaac tggtcgaac 
      gtttcaagg aaagtttta ggcctcctg gtgagccca 
      tcctacatt ttatcttaa caaacgaaa atgaaataa 
      ccaaacaag tggaaaatt ccaaagaag tgtcgcgaa 
      gaaccgcta ctacaacca ccaaaatcg ctctcaaaa 
      tactttgca acaagatgg aggtgtgta agacaaaat 
      ggctggcgc tccaagacg atttcgttt gcaacttca 
      acgaaacca tatcgattc aactaacca acgttccga 
      aattcctca cacttgaga taatcgagc aaccaaagt 
      cacttatta aaaggaccc tcggagagt tgacggtta 
      aaataccag ctcgaagtg tttcaaaaa tcgataaaa 
      aaaacgcca aatttgaga aaaacgaaa tcgttcatt 
      acaagcatg agggggtgg gaagcggca ttaaagtcg 
      actctcttg tgcaaaatt tgggcgatt ttgaactga 
      acacttcgg gttgtttga cctattcat tgtcctggg 
      atgcgtccg tggcgaatg ataacagtc cagataaca 
      gggcacaaa ttttgggtg agataatag acaagagtg 
      gaacaatag attcggaaa aactggtcc agtggatgc 
      agcagcaca accctgacc cccaagatc caagaaatt 
      cgatcttgt acgaatatt cgcttgact gtggcgagg 
      aaatggttt ctgcaagta gtaaaaact atttccggt 
      tgaacgaac aagagtggc aattttgcg acccgaaaa 
      taattgagg ataatctaa ggttcaaaa tcttgcaac 
      tgcggaaca agaatgaac cggaatttg accgaatag 
      tagtcccaa accggatca aagaaattc ctcttgatg 
      tcttagccg attatagaa ggagggtgg cgatggaac 
      gggagagtt tggttgaag aggatgaag acgatgacg 
      acgaaattg aggcaagaa ggagcaaag ggcggaagt 
      tgttgaagc aaatccaca tccgagtgt tccaaaaat 
      gcaaaaact ccgaaaata atctcagtt ttgaagacg 
      tggtagatg agaaattca tgcctcagc tggagaaac 
      tgatcaaag gctttggtg ccaatttcc ttagttggt 
      ttcagtggc ggcccttga aaccgccgt tgtttccct 
      taaaaacgc ttcaagaat cgaaatcaa acgattacg 
      atttgcaaa gtcgggtca agattaacg ttcacgtaa 
      tttcgggtt taaacattc ctctacgag agggcaaag 
      caatgcctt ttgttgaga ttcagtacg gcaatgcta 
      cagttcgaa ctgttgcaa gtcagggag ctcacccga 
      actggaacg agaaattgg ttttgccac ttgaaccgt 
      ctcatttgg attatttga atccaaatt cgcttagtg 
      gtagtttga ttttgaacg ttttcgatg aaagtgaag 
      tagttttga tgaaatttg cggggcaga aaggccgca 
      catggctag ggtatgtgg aaataccct tatcagccg 
      tttgtattg gcatgcaag gcatgttta gggtcaagg 
      tgcctcaca tgttgctgg gatatcaag gcaaaactt 
      tggagacaa caagaagta actctcaaa gtaatcaac 
      aaacttact tgaatctcc acgttacta tggaaccta 
      atgttccta aacttaatc ctgcaatag acgagcttc 
      cttgtgccg acgttcctt acattcagg actacgtta 
      tgaaatgga acacggatt acaacacca cttttccaa 
      accgtaaat tttcagttt tggcaattg atgcaaacg 
      cgacaactt catgtatca tccgctaca tcaagcctc 
      tggaacctc cccaaataa acgtggaag gatttgatg 
      tgacacctg aacagtgtg cccgatttg tctccctga 
      ttcccttca ccgattgca accagtttt acaaaaata 
      tttggcttt cagttgatc gatttttgc attacatga 
      ctggctcaa tcattgacc acgctgtag cactgacat 
      gttatctcc tcgcgttaa aactggagg tttggcttt 
      tgctaggtt ttggtattc ctcatggag ccactgctt 
      atgtccttg tgaaagaat atgacaaca atcgggtcg 
      caaactttt acgtttatg acgtcgttt caggcaata 
      aaatctact tgactgatg ctttttgcc cccttcaac 
      gagtttatt gcctcgtta atgaacaca acgtgtggg 
      ccaacattc aaagaaatg acgatgtga cagtgatga 
      gatttgatt taacgcgac gttctgatt ggttacctc 
      tttttacca gtcaaatat cagctccaa ccaattcaa 
      ttcatggaa aaattacgt acttgtctc acacaaata 
      cagacgagt tggaaatta ttcttgaca ggaaaattc 
      ggaagcgtt tcgccaagc tgaggcaac ttgatcgaa 
      ctctttgga accatgacg tttcaaata cttttaaaa 
      gtgttttgg gttctttcg aacaggcgg agatgtacg 
      gtaaaagta attcagatg ctttagaaa aaattaagg 
      agtgtttgg ctggttttg agatgagag gacagattt 
      tcaatttac cgttcacca actgtgcag cgattgtaa 
      aacacgtca aaacgtttg gattgcatt tggaggaag 
      atgctaacg tacagtttg catgtgctg tgtatctga 
      gggcttatc cgaatcaag ttttgactg tttgggtcg 
      ctctagctg tgattaaga ataaaaaaa ctgacgatc 
      cattacaag aggtattat aattttatc ctttgtaag 
      gttcgaaca ataaaatga agctttttt aaccaaatt 
      gtatcattc aaacaaata tcagatgaa taaaaagta 
      tgaaaccaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY