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Gene information
General information
TC010516 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14586359..14620444
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010516
    iBeetle screen iB_04860

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010516 RB
      MTASLDNNT VAGGDNADK GKDDVYTVS MTGGYDNKG 
      FKGDGDNGW PGKPPNKEI TDEEASSQG KFKLSTKEK 
      WRILKNVSC ISCAFMIQF TAFQGTANL QSSINAKDG 
      LGTVSLCAI YAALVVSCI FVPTFLIKR LTVKWTLCI 
      SLLCYAPYI GSQFYPRFY TLVPAGVLL GIGAAPMWA 
      SKATYLTQV AGVYAKLTD QAVDGIIVR FFGFFFLAW 
      QTSELWGNL ISSLVLSNP HGGGGASTN SAGNISYTR 
      CGSEFCTAV GNENDNLAR PDDKEIYEI SAIYLACIF 
      SAVLLIAVS VDPLSRYGE KQRRNSGVE LTGLQLLSA 
      TAYQLKKPY QQLLIPITV WIGMEQAFI GADFTQAFV 
      SCALGISSV GYVMISFGV VNAICCLLF GSVMKYIGR 
      SPIMIFGAV VHTALQVWL LIWRPHPDN PIIFFIAAG 
      MWGVGDAVW QTQVNGLYG TLFRRNKEP AFSNYRLWE 
      SVGFVITYA YSTHICAAM KLYIQLTIL ISGFLMYGM 
      VEVHHMRKV RRQKEKEKR AAELEAANK AAIQEPEET 
      DDEKDDIDD EIIVTHL
    CDS sequence
    • >TC010516 RB
      atgaccgcg tccctcgac aacaacacc gtagccggt 
      ggcgacaac gccgacaaa ggcaaggat gacgtctac 
      accgtctcg atgaccggc ggctacgac aacaagggc 
      ttcaaaggc gacggcgac aacggctgg cccgggaag 
      cccccgaac aaagagatc accgacgaa gaagcttcc 
      tcccagggc aaattcaaa ctctcgacc aaggagaaa 
      tggcggata ctgaagaac gtgtcgtgc atctcctgc 
      gccttcatg atccaattc acggccttc caaggcact 
      gccaacttg cagagcagc atcaacgcc aaagacggc 
      ctcgggact gtttctctg tgcgcgatc tacgctgct 
      ctcgtagtt agttgtatt ttcgttccc acattcctc 
      attaagagg ctaacagta aaatggacc ctctgtatc 
      tcgctgctg tgttacgcg ccctatatc ggctcgcag 
      ttctacccc aggttctac actctggtg ccggcgggg 
      gtgctgctg ggcatcggc gcggcgccc atgtgggcc 
      tccaaggcc acctatctc acgcaagtg gctggcgtc 
      tacgccaag ctcaccgac caggccgtg gacggaatc 
      atcgtcagg ttcttcggg ttctttttc ctggcttgg 
      cagacctcc gaattgtgg ggcaacttg atttcgtcg 
      ctcgtcttg agcaaccct catggaggc ggcggcgcg 
      tctacaaac tcagccggc aatatttcg tacaccagg 
      tgtggctcc gaattctgc acagcggtg ggcaatgag 
      aatgataat cttgcaaga cctgacgat aaagaaatt 
      tacgaaatc tcggcaatt tacttggcc tgcattttc 
      tcagcggtt ttacttatc gccgtttcg gtggatcct 
      ctatcaaga tacggtgaa aagcagcgt cgcaacagc 
      ggcgtggag ttgaccggt cttcagctg ctctccgcc 
      actgcctac cagttgaag aagccctac cagcagttg 
      ctcatcccc atcaccgta tggatcggc atggagcag 
      gcgttcatc ggcgccgat ttcacacag gcgttcgtt 
      tcctgcgcg ctgggcatc tcctccgtc ggctatgtc 
      atgatcagc ttcggggtg gtgaacgcc atctgctgc 
      ttgctgttc ggctcggtg atgaaatac atcgggcgc 
      tcgcccatc atgatcttc ggggcggtc gtccacacc 
      gccctccag gtctggctg ctcatctgg cggccgcac 
      cccgacaac cccatcatc ttcttcatc gcagcggga 
      atgtggggc gtcggagac gccgtctgg caaacgcaa 
      gtcaacggc ctttacggc accctcttc cgaaggaac 
      aaagaaccg gccttctcc aactatcga ctctgggag 
      agcgtcggc ttcgtcatc acttacgcc tactccacg 
      cacatttgc gctgccatg aagctctac atccagctc 
      acaatcctc atcagcggc ttcctcatg tacggcatg 
      gtcgaagtg caccacatg cgtaaagtc aggaggcag 
      aaggagaag gagaagagg gccgcagaa ctcgaagcc 
      gccaacaag gcggccatc caagaaccc gaagaaacc 
      gacgacgaa aaagacgat attgatgac gagatcatc 
      gttacacat ttg
    mRNA sequence
    • >TC010516 RB
      taagtccta attgttaaa ctgttatct tcgacattc 
      gtaaaggag tagtcattt tctttaccc gtaatgaca 
      tagcaattt aattataat tgcgttcta gtacagttc 
      tgtcagaaa ttaagtaag gttaagtct ttcgtaaat 
      agtgatttc agttgttaa ggcatctcc agccgaaaa 
      tccgagctg caaggttaa gcctcgtca agaaccggt 
      acgccatcc tcttgtact ttttggcga gccccttct 
      gttgccagg gcccctcta gcccacaag tggcaaatc 
      aaaaatcgt cgtcgcttt tccccccat ttggcagtc 
      ttgacgaaa acacaacga cgagcacaa acattgtaa 
      attaagcgg tcttatcag gagcgtgac gtgcgctgg 
      atccatcta tcagtccgg cagcttcat ctgcgagcg 
      gcacctcag cagcatccc cggcacgtg aactatctc 
      cgtaaaagc tggtcctgg gggaaaggt ctcgcagtt 
      ccaacctcg gcacttgtg gtcggtgag ccagtacac 
      acctagctc ttgttcgcg gtgcatgtg gtggacgtt 
      acggagcgt aaccagtta caaccggct cttcagttt 
      cctcgattc gacatattg gagccgatt tccccgaac 
      aaagaccta gccagtccg aaactgggg cgcatcacc 
      aataagtta aacataagt ggctgtgat ttttccgcg 
      gtggtttgc agagacctc aacgaaccg aaacgcacc 
      aggagctgc tctccggga cgacggcgg agactccgg 
      ggattcact aacaagcga caactgctg cgccgaaac 
      ggcggttga tttcctaca cacgcacaa atcgaggag 
      ggtgatcgc ggccgcgag atgaccgcg tccctcgac 
      aacaacacc gtagccggt ggcgacaac gccgacaaa 
      ggcaaggat gacgtctac accgtctcg atgaccggc 
      ggctacgac aacaagggc ttcaaaggc gacggcgac 
      aacggctgg cccgggaag cccccgaac aaagagatc 
      accgacgaa gaagcttcc tcccagggc aaattcaaa 
      ctctcgacc aaggagaaa tggcggata ctgaagaac 
      gtgtcgtgc atctcctgc gccttcatg atccaattc 
      acggccttc caaggcact gccaacttg cagagcagc 
      atcaacgcc aaagacggc ctcgggact gtttctctg 
      tgcgcgatc tacgctgct ctcgtagtt agttgtatt 
      ttcgttccc acattcctc attaagagg ctaacagta 
      aaatggacc ctctgtatc tcgctgctg tgttacgcg 
      ccctatatc ggctcgcag ttctacccc aggttctac 
      actctggtg ccggcgggg gtgctgctg ggcatcggc 
      gcggcgccc atgtgggcc tccaaggcc acctatctc 
      acgcaagtg gctggcgtc tacgccaag ctcaccgac 
      caggccgtg gacggaatc atcgtcagg ttcttcggg 
      ttctttttc ctggcttgg cagacctcc gaattgtgg 
      ggcaacttg atttcgtcg ctcgtcttg agcaaccct 
      catggaggc ggcggcgcg tctacaaac tcagccggc 
      aatatttcg tacaccagg tgtggctcc gaattctgc 
      acagcggtg ggcaatgag aatgataat cttgcaaga 
      cctgacgat aaagaaatt tacgaaatc tcggcaatt 
      tacttggcc tgcattttc tcagcggtt ttacttatc 
      gccgtttcg gtggatcct ctatcaaga tacggtgaa 
      aagcagcgt cgcaacagc ggcgtggag ttgaccggt 
      cttcagctg ctctccgcc actgcctac cagttgaag 
      aagccctac cagcagttg ctcatcccc atcaccgta 
      tggatcggc atggagcag gcgttcatc ggcgccgat 
      ttcacacag gcgttcgtt tcctgcgcg ctgggcatc 
      tcctccgtc ggctatgtc atgatcagc ttcggggtg 
      gtgaacgcc atctgctgc ttgctgttc ggctcggtg 
      atgaaatac atcgggcgc tcgcccatc atgatcttc 
      ggggcggtc gtccacacc gccctccag gtctggctg 
      ctcatctgg cggccgcac cccgacaac cccatcatc 
      ttcttcatc gcagcggga atgtggggc gtcggagac 
      gccgtctgg caaacgcaa gtcaacggc ctttacggc 
      accctcttc cgaaggaac aaagaaccg gccttctcc 
      aactatcga ctctgggag agcgtcggc ttcgtcatc 
      acttacgcc tactccacg cacatttgc gctgccatg 
      aagctctac atccagctc acaatcctc atcagcggc 
      ttcctcatg tacggcatg gtcgaagtg caccacatg 
      cgtaaagtc aggaggcag aaggagaag gagaagagg 
      gccgcagaa ctcgaagcc gccaacaag gcggccatc 
      caagaaccc gaagaaacc gacgacgaa aaagacgat 
      attgatgac gagatcatc gttacacat ttgtagaaa 
      ggtgagcat cactttgca aaagaagtt atatcaagt 
      gtggctgca cgcccgaaa ttggcgcag tcagtagga 
      tttgttaaa cgcatggct tccttgatg catttagcg 
      gactgacgt ttatttgac aaataccat ttttgaggt 
      taaagacta ataaatttg aaagatgta a
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