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Gene information
General information
TC010508 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15119597..15127098
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010508
    iBeetle screen iB_04859

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010508 RA
      MATQSEDEK KNKVCQNYM KDENFNDSS FNKCGEILE 
      KCNLSDSSS DYLSTLEEN NEGNVLNIK SENVCFTNK 
      MIATNNQLQ QDFLTIDNI LSKGPNQYN NIQIYKSQN 
      VHIGDVTHI NGPVYINHL TPTINQNIV INTNTNNDE 
      DYPIVARRT WLAQPPLDP DDVKFFKKP PKFVIICHS 
      ASEEAYTQT DNNLLVRLI QQFHVESRK WNDISYNFL 
      VGAEGSVYE GRGWKTVGA HTQGYNSVS IGICFIGCY 
      IQNLPPSVA LRKAKELIR YGVKIGAIS EDYTLLGHC 
      QCRSTESPG RRLFEEIKS WERWDGKIS LENPSPLIE
    CDS sequence
    • >TC010508 RA
      atggctacg caaagcgag gacgagaag aaaaataaa 
      gtttgccaa aactacatg aaagacgaa aatttcaac 
      gactcatct tttaataaa tgtggtgaa attttagaa 
      aagtgtaac ttaagtgat tcatctagc gattattta 
      tcaacattg gaggaaaac aatgaagga aatgtgtta 
      aacatcaag agcgaaaat gtttgtttt accaacaaa 
      atgatagct accaataac caactgcag caagacttt 
      ttgactata gacaacatt ctaagcaaa ggaccaaac 
      cagtataac aatattcaa atctacaaa tcgcaaaat 
      gtccacatc ggtgacgtt actcacata aatggacct 
      gtttacata aatcaccta acgccaaca ataaatcaa 
      aatattgta ataaacacc aacactaac aacgacgaa 
      gattatccc attgtggca cgtcgaacc tggctggcg 
      cagccccct ttggatccc gacgatgtc aaattcttc 
      aaaaaaccg ccgaagttt gtgataatt tgccattcg 
      gcaagcgaa gaagcctac acacaaacg gataacaac 
      ctccttgtc cgtctaatt caacaattt cacgtcgaa 
      agccgcaaa tggaacgac atttcgtac aattttctt 
      gttggagcc gaaggaagc gtttatgag ggacgcggc 
      tggaaaacc gtcggtgct cacacgcaa gggtacaac 
      tctgtgtcc atcggcatt tgcttcatt ggctgttat 
      atccaaaac ctgccaccg tctgtagcg ttgcgtaag 
      gccaaggag ttgattcgg tatggagtc aagattggg 
      gccatttcg gaagattac acccttttg ggacactgc 
      cagtgcaga agtaccgaa agccccggc cgtcgcttg 
      tttgaagaa atcaagagt tgggaacgg tgggacggc 
      aagataagt ttggaaaac ccatctccg ttgattgag 
      tga
    mRNA sequence
    • >TC010508 RA
      tacaaaaca ataaacatt ggtagtaat cggtgatgt 
      cctgatgtg tgaaaagga gtcgatttg ttaaaatca 
      aataaatta aaaactgag tgaataata atcatggct 
      acgcaaagc gaggacgag aagaaaaat aaagtttgc 
      caaaactac atgaaagac gaaaatttc aacgactca 
      tcttttaat aaatgtggt gaaatttta gaaaagtgt 
      aacttaagt gattcatct agcgattat ttatcaaca 
      ttggaggaa aacaatgaa ggaaatgtg ttaaacatc 
      aagagcgaa aatgtttgt tttaccaac aaaatgata 
      gctaccaat aaccaactg cagcaagac tttttgact 
      atagacaac attctaagc aaaggacca aaccagtat 
      aacaatatt caaatctac aaatcgcaa aatgtccac 
      atcggtgac gttactcac ataaatgga cctgtttac 
      ataaatcac ctaacgcca acaataaat caaaatatt 
      gtaataaac accaacact aacaacgac gaagattat 
      cccattgtg gcacgtcga acctggctg gcgcagccc 
      cctttggat cccgacgat gtcaaattc ttcaaaaaa 
      ccgccgaag tttgtgata atttgccat tcggcaagc 
      gaagaagcc tacacacaa acggataac aacctcctt 
      gtccgtcta attcaacaa tttcacgtc gaaagccgc 
      aaatggaac gacatttcg tacaatttt cttgttgga 
      gccgaagga agcgtttat gagggacgc ggctggaaa 
      accgtcggt gctcacacg caagggtac aactctgtg 
      tccatcggc atttgcttc attggctgt tatatccaa 
      aacctgcca ccgtctgta gcgttgcgt aaggccaag 
      gagttgatt cggtatgga gtcaagatt ggggccatt 
      tcggaagat tacaccctt ttgggacac tgccagtgc 
      agaagtacc gaaagcccc ggccgtcgc ttgtttgaa 
      gaaatcaag agttgggaa cggtgggac ggcaagata 
      agtttggaa aacccatct ccgttgatt gagtgatgt 
      aaagaaatt tttattaaa ctgtgtcca caatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY