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Gene information
General information
TC010507 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15136643..15141618
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010507
    iBeetle screen iB_04858

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010507 RA
      MFLPIVVAL LLLVTTFYI HSKRPNPPK IFNVYSQPG 
      KWFYLKFAA FYLLVWTRR LKSKLRKGT PVHELEKMK 
      PLSDHEKAF DATFFQAVS QNGFFFCGG IERRHRAKA 
      NGLFYIAVP ELGLLESEK LPSTLLESD PGSVLLLKD 
      YSAEGISFT PVEPMKKWH VAYNGKMRI QNEPHKFVS 
      VKLNGDWTS NLPHFLFES DINIKCLAK AIARETWNE 
      ELFKALKSA HQTHYEQMG YFNGTLKVG NKTYTLKMD 
      AFRDHSFGY KRDWTLMHR YAFHMLYLK NGTRVSFGV 
      VSQPCTTSL LEMGYIVLP DKSIHCIDS CDLLLYQHG 
      ENGTPGTSY AFSFSANNE TYDARIKVI YESVHFKGN 
      NIEARLIER FIECEVNGM PGKGVSEWQ YNNMKNLRK 
      V
    CDS sequence
    • >TC010507 RA
      atgttccta ccgatagta gtcgcccta ctgctcctc 
      gtaacgacc ttctacatc cacagcaaa agaccaaac 
      ccaccaaaa attttcaac gtatattcg caaccggga 
      aaatggttt tatttaaaa tttgccgca ttctacctc 
      ctcgtatgg acaagacga ctcaaatcg aaattacgt 
      aaaggaact cccgtccat gagttagaa aaaatgaaa 
      ccgttatcc gaccatgaa aaggcattt gatgcaaca 
      ttctttcaa gccgtttcc cagaatggt ttctttttt 
      tgcggcggt atcgaacga cgacatcga gctaaagct 
      aatgggtta ttctatata gcggtaccg gaattgggg 
      ctcctggaa agtgagaaa ttaccaagc actttactt 
      gaatctgac ccggggtca gttttgtta cttaaagat 
      tatagcgcg gaggggatt tctttcaca ccggtggag 
      cccatgaaa aaatggcac gtggcttac aacggaaaa 
      atgagaata caaaacgaa ccccataaa tttgtcagt 
      gttaaactc aacggcgac tggacatcg aatttgcct 
      cattttctc tttgagagt gacataaat atcaaatgt 
      ttggctaag gccatagcg agagagact tggaatgag 
      gagctcttc aaggcacta aaatcagcc caccaaact 
      cactacgaa caaatgggt tactttaac gggactctg 
      aaggttggg aataagaca tatacgtta aagatggat 
      gctttccgg gatcatagt ttcggttat aaaagagac 
      tggactttg atgcaccgt tacgctttt cacatgttg 
      tacttaaaa aatggtaca agagtgtct tttggagtg 
      gtaagtcaa ccttgcaca acgtcgctc ttggagatg 
      ggatacata gttttacct gacaagtca atccattgc 
      atcgattcc tgcgattta cttctatac caacatggt 
      gaaaatggc actcctggg accagttac gctttctcg 
      ttcagcgca aataatgag acttacgac gctagaatt 
      aaagtaatt tatgaatca gtccatttt aaaggaaac 
      aacattgaa gcacgattg attgaaaga tttatagag 
      tgtgaagtg aatgggatg ccgggaaaa ggtgtctct 
      gaatggcaa tacaacaat atgaaaaac cttcgtaaa 
      gtataa
    mRNA sequence
    • >TC010507 RA
      cacatcaca aaatgttcc taccgatag tagtcgccc 
      tactgctcc tcgtaacga ccttctaca tccacagca 
      aaagaccaa acccaccaa aaattttca acgtatatt 
      cgcaaccgg gaaaatggt tttatttaa aatttgccg 
      cattctacc tcctcgtat ggacaagac gactcaaat 
      cgaaattac gtaaaggaa ctcccgtcc atgagttag 
      aaaaaatga aaccgttat ccgaccatg aaaaggcat 
      ttgatgcaa cattctttc aagccgttt cccagaatg 
      gtttctttt tttgcggcg gtatcgaac gacgacatc 
      gagctaaag ctaatgggt tattctata tagcggtac 
      cggaattgg ggctcctgg aaagtgaga aattaccaa 
      gcactttac ttgaatctg acccggggt cagttttgt 
      tacttaaag attatagcg cggagggga tttctttca 
      caccggtgg agcccatga aaaaatggc acgtggctt 
      acaacggaa aaatgagaa tacaaaacg aaccccata 
      aatttgtca gtgttaaac tcaacggcg actggacat 
      cgaatttgc ctcattttc tctttgaga gtgacataa 
      atatcaaat gtttggcta aggccatag cgagagaga 
      cttggaatg aggagctct tcaaggcac taaaatcag 
      cccaccaaa ctcactacg aacaaatgg gttacttta 
      acgggactc tgaaggttg ggaataaga catatacgt 
      taaagatgg atgctttcc gggatcata gtttcggtt 
      ataaaagag actggactt tgatgcacc gttacgctt 
      ttcacatgt tgtacttaa aaaatggta caagagtgt 
      cttttggag tggtaagtc aaccttgca caacgtcgc 
      tcttggaga tgggataca tagttttac ctgacaagt 
      caatccatt gcatcgatt cctgcgatt tacttctat 
      accaacatg gtgaaaatg gcactcctg ggaccagtt 
      acgctttct cgttcagcg caaataatg agacttacg 
      acgctagaa ttaaagtaa tttatgaat cagtccatt 
      ttaaaggaa acaacattg aagcacgat tgattgaaa 
      gatttatag agtgtgaag tgaatggga tgccgggaa 
      aaggtgtct ctgaatggc aatacaaca atatgaaaa 
      accttcgta aagtataaa aatattctt atttaaact 
      tgaaaaaat aaaacaata taagtgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY