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Gene information
General information
TC010498 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15544359..15546341
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010498
    iBeetle screen iB_04854

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010498 RB
      MVLSAIFIL DSNGIVLMS RDYRGDVGK EQIEEFLPL 
      LNQQEELGN SSPLLHHDK VSFAYVKHE GLYITSVMK 
      NNANIALVF TFLYKFIQI ATQYFNKLE EESIRDNFV 
      ILYELLDEI MDFGYPQTT DSKILQTYI FQESYKLKK 
      APTIPAVVT NVVSWRPEG IKYRRNELF IDVIESVNL 
      SVNSSGAIL RNEVSGCVK MKVHLSGMP QLRLGLSDK 
      ILLAINSSG QESATFEDV KFHQCVQLS RICDKNVYF 
      IPPDGDFEL MSYRMNTEI KPLILVRSK VVQASTSRI 
      EYTVKVSAQ FKASSTANN VEVTLPVCQ DVDSPVFKA 
      TAGMASYVP EKAAVVWKI KYFPGGSEN LLHVCFKLS 
      TIRGEEKDD KKPIQVKFM IPYFTISGL QIKYMKVIE 
      KSNYKALTW VRYTTQNGE YLVCLI
    CDS sequence
    • >TC010498 RB
      atggtcctt tcagcaatt tttattttg gattcaaat 
      ggaatagtc ctgatgtct cgagactat cgcggagat 
      gtcggtaaa gaacaaatc gaggaattt cttccttta 
      ttaaaccaa caagaagaa ctaggaaat tcatctccg 
      ctgctgcac cacgacaaa gtttctttt gcttatgta 
      aaacacgag ggcttgtat ataacaagt gtaatgaag 
      aacaacgca aacattgcc ctagttttc actttctta 
      tacaagttt attcaaatc gcgactcaa tatttcaac 
      aaactcgag gaagaaagc atccgcgac aactttgtg 
      attctgtac gagttgttg gacgagata atggacttt 
      ggctacccg caaaccacc gattcgaaa attctccaa 
      acgtacatc ttccaagag agttataag ctgaaaaag 
      gccccaaca attcccgcc gtggtgaca aacgtggtc 
      tcgtggcgg cccgaaggc ataaaatac cggcgcaac 
      gagctgttt atcgatgta atcgaatcg gtaaactta 
      tcagtgaat tcctccggc gccatcctc cgaaacgaa 
      gtctccggt tgcgtaaaa atgaaggta catttgtcc 
      ggaatgccc caattaaga ttaggcttg agcgacaag 
      attcttctg gccatcaat tcgagcggc caagaatcg 
      gccactttc gaagacgtt aagttccac caatgcgtc 
      caattatcg cggatttgc gataaaaac gtttacttc 
      atcccacct gatggcgac ttcgagctg atgtcttat 
      cgcatgaat acggagatt aagcctttg attttggtc 
      aggagtaaa gtggtccag gcgtccacg agcaggata 
      gagtacacg gtcaaagtc agcgcacag tttaaggcc 
      tcctcgacg gcgaataat gtggaagtg actttgccg 
      gtttgtcag gatgttgat tcgccggtt tttaaggcg 
      acggcagga atggcgagc tatgtcccg gaaaaagcg 
      gcggtggtg tggaagatc aagtatttt ccgggaggg 
      agtgagaac ttattgcac gtttgtttt aaactttcg 
      acgatacga ggggaagaa aaggacgac aagaaaccc 
      atacaagtt aaatttatg attccatat tttactatt 
      tctggctta cagattaag tatatgaaa gtgattgag 
      aagagtaat tataaggct ttaacttgg gtaaggtat 
      actactcaa aatggagaa tatctagtt tgtctcatt
    mRNA sequence
    • >TC010498 RB
      ttgccatta cccactgca aagtaaaaa aaactcgtt 
      tttgttagg aaagaaaga cgtcatatt acctattgt 
      caaaatggt cctttcagc aatttttat tttggattc 
      aaatggaat agtcctgat gtctcgaga ctatcgcgg 
      agatgtcgg taaagaaca aatcgagga atttcttcc 
      tttattaaa ccaacaaga agaactagg aaattcatc 
      tccgctgct gcaccacga caaagtttc ttttgctta 
      tgtaaaaca cgagggctt gtatataac aagtgtaat 
      gaagaacaa cgcaaacat tgccctagt tttcacttt 
      cttatacaa gtttattca aatcgcgac tcaatattt 
      caacaaact cgaggaaga aagcatccg cgacaactt 
      tgtgattct gtacgagtt gttggacga gataatgga 
      ctttggcta cccgcaaac caccgattc gaaaattct 
      ccaaacgta catcttcca agagagtta taagctgaa 
      aaaggcccc aacaattcc cgccgtggt gacaaacgt 
      ggtctcgtg gcggcccga aggcataaa ataccggcg 
      caacgagct gtttatcga tgtaatcga atcggtaaa 
      cttatcagt gaattcctc cggcgccat cctccgaaa 
      cgaagtctc cggttgcgt aaaaatgaa ggtacattt 
      gtccggaat gccccaatt aagattagg cttgagcga 
      caagattct tctggccat caattcgag cggccaaga 
      atcggccac tttcgaaga cgttaagtt ccaccaatg 
      cgtccaatt atcgcggat ttgcgataa aaacgttta 
      cttcatccc acctgatgg cgacttcga gctgatgtc 
      ttatcgcat gaatacgga gattaagcc tttgatttt 
      ggtcaggag taaagtggt ccaggcgtc cacgagcag 
      gatagagta cacggtcaa agtcagcgc acagtttaa 
      ggcctcctc gacggcgaa taatgtgga agtgacttt 
      gccggtttg tcaggatgt tgattcgcc ggtttttaa 
      ggcgacggc aggaatggc gagctatgt cccggaaaa 
      agcggcggt ggtgtggaa gatcaagta ttttccggg 
      agggagtga gaacttatt gcacgtttg ttttaaact 
      ttcgacgat acgagggga agaaaagga cgacaagaa 
      acccataca agttaaatt tatgattcc atattttac 
      tatttctgg cttacagat taagtatat gaaagtgat 
      tgagaagag taattataa ggctttaac ttgggtaag 
      gtatactac tcaaaatgg agaatatct agtttgtct 
      catttagct ataacctct aatcttgta agctcttgc 
      aataaaaga aaattataa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY