| General information | |||
| TC010496 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 15582816..15592896 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010496 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04853 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
- >TC010496 RA
MYKIVVRTR PFFPKLKNL RFLSPGAPK PDGPLFPHK DEFPSRHIG PRDSDIIQM LDSLGFKSL DELTDKAVP QNIRLNRQL DIEEPVGEY ELINRIRKI AERNQIWRS YIGMGYHNC CVPHTIMRN IFENPGWTT QYTPYQPEV SQGRLEGLL NYQTMVSDL TGLDVANAS LLDEGTAAA EALSLCYRH NKRRKMLMS DKLHPQTIS VVETRLSSL GLQVEVVDV FEADFSNRD VAGVLFQYP DTEGNILDF STVTEAAHE HGTLVCCAT DLLALTLLR PPSEFGADI AVGTSQRLG VPLGYGGPH AGFFACNQS LVRLMPGRM IGVTRDAAG RDGYRLALQ TREQHIRRD KATSNICTA QALLANMSA MFAVYHGPQ GLKDIGTKI HNMTLVLSH GLQQDGNTL TNELFFDTL RIEPKLSTE EIQRRANAK KINLRYFDD GSVGVSLDE TVTFADIND LFEIFGSSH KIEDLLNNP MVREHSITK GEYKRTSPF LTHPVFNSH HSETRIVRY MKILENKDI SLVHSMIPL GSCTMKLNS TTEMMPCSF KHFTDIHPF APLDQTLGY HQLFAELEK DLCAITGYD KISFQPNSG AQGEYAGLR AIQCYHEAR GDKNRDVCL IPVSAHGTN PASAQMAGM RIEPVRVKH DGSIDVEDL KAKAEKFNS RLSCMMITY PSTNGLFEE TVADVCDII HKNGGQVYL DGANMNAQV GLCRPGDYG GDVSHLNLH KTFCIPHGG GGPGMGPIG VKSHLAPFL PGHPVVNPL GEDSPTYGV VSAAPFGSS AILPISWAY IKMMGARGL RKATQVAIL NANYMSKVL EQHYTTLFK SPTSDLVAH EFIIDTREF KKTANIEAA DIAKRLMDY GFHAPTMSW PVAGTLMIE PTESEDKQE LDRFCESLI SIRQEIKDI EDGVMDKRV NPLKMAPHT QEQVINSAW ERPYTREQA AFPAPFVRP EVKVWPTVA RIDDIYGDK HLVCTCPPI LDEYNY
- >TC010496 RA
atgtacaaa attgtagtc cgaacccgc ccttttttc cccaaacta aaaaatttg cgatttcta agtcccggt gctccaaaa cccgacggt cctttattt ccacacaaa gatgaattc cctagtcga cacatcggt cctagggac agcgacatt atccaaatg ttggactcg ctcggtttt aagagtttg gacgagttg acggacaag gccgttcca caaaacatt cgcttgaat cgacaattg gacattgaa gagcccgta ggcgagtac gagctgata aatcgaata cgaaaaata gcggaacga aatcaaatt tggaggtca tacattggc atggggtac cacaattgt tgcgtaccg cataccatc atgcgaaat atttttgaa aatccaggc tggaccact cagtatact ccttatcag ccggaggtg tcgcaaggg agactcgaa gggttgctc aactaccag acaatggtc tctgacttg acgggtttg gatgtggct aatgcctca cttttggac gaagggaca gcagcagct gaagccctt tcgctttgt tatcgacat aataagagg cgaaagatg cttatgtct gataaactt cacccacaa acaatttca gtcgttgag acacgttta tcgtctttg ggacttcaa gtcgaagtt gtggacgtt ttcgaagct gatttctcg aacagagac gttgcagga gttcttttc caatatcca gacactgaa ggaaacatt cttgatttt agtacggtc acagaagct gcacatgaa cacggtact ctagtgtgt tgcgctaca gatctgctc gcgttaact cttctccga ccaccgagt gagtttggt gctgatatt gctgttggt acttcgcaa cggttgggg gtaccattg ggttatgga gggccccat gctgggttt tttgcttgt aatcagtcc ttggtgcga cttatgcca ggcaggatg attggagtg acacgagat gctgctggt cgagatgga tatcgtttg gcattacag acacgtgaa caacacatc agaagagat aaggctaca agtaatatt tgtaccgct caggcgtta ctagcgaat atgtcggcc atgtttgcg gtgtaccat ggaccacaa gggcttaaa gatattggt actaaaata cacaacatg acgttggta ttaagccac ggcttgcag caagacggt aacacctta acgaacgaa ctcttcttc gacactctc cgaatcgaa ccaaaactc tccaccgaa gagatccaa cgtcgagcc aacgcgaaa aaaatcaac ctacgatat ttcgacgac ggctcagtc ggagtctcc ctcgacgaa acagtcact ttcgccgac attaacgac ctgtttgaa atattcggc agttcgcac aaaatcgaa gacttgctc aacaaccca atggtgcgc gaacactca atcaccaaa ggcgaatac aaaagaacc tccccgttt ctaacacac ccagtattt aactcacac cattccgaa actaggatc gttcgctac atgaaaatc ttggagaat aaggacatt tctctggtc cattcgatg atcccgctt ggttcttgc acaatgaaa cttaactca acgacagag atgatgcct tgttccttc aaacacttt actgatatt catccgttt gcgcctttg gatcaaact ttggggtat caccagttg tttgcagag cttgagaaa gatctttgt gccatcact gggtatgac aagattagt ttccagccg aatagtggc gcccaaggt gaatatgca ggtctgagg gccattcag tgctaccat gaagcccga ggtgataaa aatcgggac gtttgtttg attcctgtg agtgcccat gggacgaac ccagcaagt gcccaaatg gccgggatg cggattgaa ccagtcaga gtgaaacat gatggttcg atcgatgtc gaagatctt aaagcgaag gccgagaag ttcaacagt cgtctctcg tgcatgatg atcacctac ccatcaact aatggactt tttgaggaa acggtcgca gatgtgtgt gatattatc cataaaaac ggtggtcag gtctaccta gatggtgcc aacatgaac gcacaagtc ggattgtgc cgacctggt gattacggc ggtgacgtc tcccatctg aacttgcac aaaaccttc tgcataccg catggaggt ggtggtcct ggtatgggt ccaatcggg gtcaagtca cacctagca ccctttttg ccgggtcat cccgtcgtc aaccccttg ggcgaagac tccccaact tatggtgtt gtaagtgcc gcacctttc ggttcctct gccattctc cccatttcg tgggcttat atcaaaatg atgggcgct cgaggtttg aggaaagcc acccaagtc gcgatacta aacgccaat tacatgtcg aaagtatta gaacaacac tacaccact ttgtttaag agcccaacg tcggatttg gtcgctcac gagtttatt attgacaca agagagttt aaaaagact gccaatatc gaagctgca gatattgcg aaaagactg atggattat gggttccat gcgcccacc atgtcctgg cctgtggct gggactttg atgattgaa ccgacggaa tcggaggat aagcaggag ttggaccgg ttttgtgaa tcgttgatt tcgattaga caggagatt aaggatatt gaggacggg gttatggat aaaagggtg aatccgttg aagatggcg ccgcatacg caagaacag gttattaac agtgcttgg gagaggcca tatacgagg gaacaggcg gcgtttccg gctccgttt gtgaggcca gaagttaaa gtttggccg acggttgca aggattgat gatatttat ggcgataag catctcgtg tgtacgtgc ccccccatt ttggacgaa tacaattac taa
- >TC010496 RA
ttatcatcc aagcaatca ttctgtaac agatgctat tcaatatgc aaacacgtc catttatca cacaattac tccacaatt tacataaaa attatcttg ctcttatca tatttttgc gacccctga ccccatcca tttatattg acaatgtga agtcgaaaa taattagtt cagtcgaga tcgattaat taactaaca agatgtaca aaattgtag tccgaaccc gcccttttt tccccaaac taaaaaatt tgcgatttc taagtcccg gtgctccaa aacccgacg gtcctttat ttccacaca aagatgaat tccctagtc gacacatcg gtcctaggg acagcgaca ttatccaaa tgttggact cgctcggtt ttaagagtt tggacgagt tgacggaca aggccgttc cacaaaaca ttcgcttga atcgacaat tggacattg aagagcccg taggcgagt acgagctga taaatcgaa tacgaaaaa tagcggaac gaaatcaaa tttggaggt catacattg gcatggggt accacaatt gttgcgtac cgcatacca tcatgcgaa atatttttg aaaatccag gctggacca ctcagtata ctccttatc agccggagg tgtcgcaag ggagactcg aagggttgc tcaactacc agacaatgg tctctgact tgacgggtt tggatgtgg ctaatgcct cacttttgg acgaaggga cagcagcag ctgaagccc tttcgcttt gttatcgac ataataaga ggcgaaaga tgcttatgt ctgataaac ttcacccac aaacaattt cagtcgttg agacacgtt tatcgtctt tgggacttc aagtcgaag ttgtggacg ttttcgaag ctgatttct cgaacagag acgttgcag gagttcttt tccaatatc cagacactg aaggaaaca ttcttgatt ttagtacgg tcacagaag ctgcacatg aacacggta ctctagtgt gttgcgcta cagatctgc tcgcgttaa ctcttctcc gaccaccga gtgagtttg gtgctgata ttgctgttg gtacttcgc aacggttgg gggtaccat tgggttatg gagggcccc atgctgggt tttttgctt gtaatcagt ccttggtgc gacttatgc caggcagga tgattggag tgacacgag atgctgctg gtcgagatg gatatcgtt tggcattac agacacgtg aacaacaca tcagaagag ataaggcta caagtaata tttgtaccg ctcaggcgt tactagcga atatgtcgg ccatgtttg cggtgtacc atggaccac aagggctta aagatattg gtactaaaa tacacaaca tgacgttgg tattaagcc acggcttgc agcaagacg gtaacacct taacgaacg aactcttct tcgacactc tccgaatcg aaccaaaac tctccaccg aagagatcc aacgtcgag ccaacgcga aaaaaatca acctacgat atttcgacg acggctcag tcggagtct ccctcgacg aaacagtca ctttcgccg acattaacg acctgtttg aaatattcg gcagttcgc acaaaatcg aagacttgc tcaacaacc caatggtgc gcgaacact caatcacca aaggcgaat acaaaagaa cctccccgt ttctaacac acccagtat ttaactcac accattccg aaactagga tcgttcgct acatgaaaa tcttggaga ataaggaca tttctctgg tccattcga tgatcccgc ttggttctt gcacaatga aacttaact caacgacag agatgatgc cttgttcct tcaaacact ttactgata ttcatccgt ttgcgcctt tggatcaaa ctttggggt atcaccagt tgtttgcag agcttgaga aagatcttt gtgccatca ctgggtatg acaagatta gtttccagc cgaatagtg gcgcccaag gtgaatatg caggtctga gggccattc agtgctacc atgaagccc gaggtgata aaaatcggg acgtttgtt tgattcctg tgagtgccc atgggacga acccagcaa gtgcccaaa tggccggga tgcggattg aaccagtca gagtgaaac atgatggtt cgatcgatg tcgaagatc ttaaagcga aggccgaga agttcaaca gtcgtctct cgtgcatga tgatcacct acccatcaa ctaatggac tttttgagg aaacggtcg cagatgtgt gtgatatta tccataaaa acggtggtc aggtctacc tagatggtg ccaacatga acgcacaag tcggattgt gccgacctg gtgattacg gcggtgacg tctcccatc tgaacttgc acaaaacct tctgcatac cgcatggag gtggtggtc ctggtatgg gtccaatcg gggtcaagt cacacctag caccctttt tgccgggtc atcccgtcg tcaacccct tgggcgaag actccccaa cttatggtg ttgtaagtg ccgcacctt tcggttcct ctgccattc tccccattt cgtgggctt atatcaaaa tgatgggcg ctcgaggtt tgaggaaag ccacccaag tcgcgatac taaacgcca attacatgt cgaaagtat tagaacaac actacacca ctttgttta agagcccaa cgtcggatt tggtcgctc acgagttta ttattgaca caagagagt ttaaaaaga ctgccaata tcgaagctg cagatattg cgaaaagac tgatggatt atgggttcc atgcgccca ccatgtcct ggcctgtgg ctgggactt tgatgattg aaccgacgg aatcggagg ataagcagg agttggacc ggttttgtg aatcgttga tttcgatta gacaggaga ttaaggata ttgaggacg gggttatgg ataaaaggg tgaatccgt tgaagatgg cgccgcata cgcaagaac aggttatta acagtgctt gggagaggc catatacga gggaacagg cggcgtttc cggctccgt ttgtgaggc cagaagtta aagtttggc cgacggttg caaggattg atgatattt atggcgata agcatctcg tgtgtacgt gccccccca ttttggacg aatacaatt actaaatgt ttggatcag aatgtatta tttattgaa tcgacgcta caataattt ttaaggaaa ttggaattt tttctgaag ctgcgcaaa atcaaatct agtcatcat attcttatt tgcgattat tgtacacgt aacaatgaa ttcgagtca gactttgcc taattatgt acataatac ctaataata acaataata ttaacagaa atac
