Status
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Gene information
General information
TC010496 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15582816..15592896
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010496
    iBeetle screen iB_04853

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010496 RA
      MYKIVVRTR PFFPKLKNL RFLSPGAPK PDGPLFPHK 
DEFPSRHIG PRDSDIIQM LDSLGFKSL DELTDKAVP 
QNIRLNRQL DIEEPVGEY ELINRIRKI AERNQIWRS 
YIGMGYHNC CVPHTIMRN IFENPGWTT QYTPYQPEV 
SQGRLEGLL NYQTMVSDL TGLDVANAS LLDEGTAAA 
EALSLCYRH NKRRKMLMS DKLHPQTIS VVETRLSSL 
GLQVEVVDV FEADFSNRD VAGVLFQYP DTEGNILDF 
STVTEAAHE HGTLVCCAT DLLALTLLR PPSEFGADI 
AVGTSQRLG VPLGYGGPH AGFFACNQS LVRLMPGRM 
IGVTRDAAG RDGYRLALQ TREQHIRRD KATSNICTA 
QALLANMSA MFAVYHGPQ GLKDIGTKI HNMTLVLSH 
GLQQDGNTL TNELFFDTL RIEPKLSTE EIQRRANAK 
KINLRYFDD GSVGVSLDE TVTFADIND LFEIFGSSH 
KIEDLLNNP MVREHSITK GEYKRTSPF LTHPVFNSH 
HSETRIVRY MKILENKDI SLVHSMIPL GSCTMKLNS 
TTEMMPCSF KHFTDIHPF APLDQTLGY HQLFAELEK 
DLCAITGYD KISFQPNSG AQGEYAGLR AIQCYHEAR 
GDKNRDVCL IPVSAHGTN PASAQMAGM RIEPVRVKH 
DGSIDVEDL KAKAEKFNS RLSCMMITY PSTNGLFEE 
TVADVCDII HKNGGQVYL DGANMNAQV GLCRPGDYG 
GDVSHLNLH KTFCIPHGG GGPGMGPIG VKSHLAPFL 
PGHPVVNPL GEDSPTYGV VSAAPFGSS AILPISWAY 
IKMMGARGL RKATQVAIL NANYMSKVL EQHYTTLFK 
SPTSDLVAH EFIIDTREF KKTANIEAA DIAKRLMDY 
GFHAPTMSW PVAGTLMIE PTESEDKQE LDRFCESLI 
SIRQEIKDI EDGVMDKRV NPLKMAPHT QEQVINSAW 
ERPYTREQA AFPAPFVRP EVKVWPTVA RIDDIYGDK 
HLVCTCPPI LDEYNY
    CDS sequence
    • >TC010496 RA
      atgtacaaa attgtagtc cgaacccgc ccttttttc 
cccaaacta aaaaatttg cgatttcta agtcccggt 
gctccaaaa cccgacggt cctttattt ccacacaaa 
gatgaattc cctagtcga cacatcggt cctagggac 
agcgacatt atccaaatg ttggactcg ctcggtttt 
aagagtttg gacgagttg acggacaag gccgttcca 
caaaacatt cgcttgaat cgacaattg gacattgaa 
gagcccgta ggcgagtac gagctgata aatcgaata 
cgaaaaata gcggaacga aatcaaatt tggaggtca 
tacattggc atggggtac cacaattgt tgcgtaccg 
cataccatc atgcgaaat atttttgaa aatccaggc 
tggaccact cagtatact ccttatcag ccggaggtg 
tcgcaaggg agactcgaa gggttgctc aactaccag 
acaatggtc tctgacttg acgggtttg gatgtggct 
aatgcctca cttttggac gaagggaca gcagcagct 
gaagccctt tcgctttgt tatcgacat aataagagg 
cgaaagatg cttatgtct gataaactt cacccacaa 
acaatttca gtcgttgag acacgttta tcgtctttg 
ggacttcaa gtcgaagtt gtggacgtt ttcgaagct 
gatttctcg aacagagac gttgcagga gttcttttc 
caatatcca gacactgaa ggaaacatt cttgatttt 
agtacggtc acagaagct gcacatgaa cacggtact 
ctagtgtgt tgcgctaca gatctgctc gcgttaact 
cttctccga ccaccgagt gagtttggt gctgatatt 
gctgttggt acttcgcaa cggttgggg gtaccattg 
ggttatgga gggccccat gctgggttt tttgcttgt 
aatcagtcc ttggtgcga cttatgcca ggcaggatg 
attggagtg acacgagat gctgctggt cgagatgga 
tatcgtttg gcattacag acacgtgaa caacacatc 
agaagagat aaggctaca agtaatatt tgtaccgct 
caggcgtta ctagcgaat atgtcggcc atgtttgcg 
gtgtaccat ggaccacaa gggcttaaa gatattggt 
actaaaata cacaacatg acgttggta ttaagccac 
ggcttgcag caagacggt aacacctta acgaacgaa 
ctcttcttc gacactctc cgaatcgaa ccaaaactc 
tccaccgaa gagatccaa cgtcgagcc aacgcgaaa 
aaaatcaac ctacgatat ttcgacgac ggctcagtc 
ggagtctcc ctcgacgaa acagtcact ttcgccgac 
attaacgac ctgtttgaa atattcggc agttcgcac 
aaaatcgaa gacttgctc aacaaccca atggtgcgc 
gaacactca atcaccaaa ggcgaatac aaaagaacc 
tccccgttt ctaacacac ccagtattt aactcacac 
cattccgaa actaggatc gttcgctac atgaaaatc 
ttggagaat aaggacatt tctctggtc cattcgatg 
atcccgctt ggttcttgc acaatgaaa cttaactca 
acgacagag atgatgcct tgttccttc aaacacttt 
actgatatt catccgttt gcgcctttg gatcaaact 
ttggggtat caccagttg tttgcagag cttgagaaa 
gatctttgt gccatcact gggtatgac aagattagt 
ttccagccg aatagtggc gcccaaggt gaatatgca 
ggtctgagg gccattcag tgctaccat gaagcccga 
ggtgataaa aatcgggac gtttgtttg attcctgtg 
agtgcccat gggacgaac ccagcaagt gcccaaatg 
gccgggatg cggattgaa ccagtcaga gtgaaacat 
gatggttcg atcgatgtc gaagatctt aaagcgaag 
gccgagaag ttcaacagt cgtctctcg tgcatgatg 
atcacctac ccatcaact aatggactt tttgaggaa 
acggtcgca gatgtgtgt gatattatc cataaaaac 
ggtggtcag gtctaccta gatggtgcc aacatgaac 
gcacaagtc ggattgtgc cgacctggt gattacggc 
ggtgacgtc tcccatctg aacttgcac aaaaccttc 
tgcataccg catggaggt ggtggtcct ggtatgggt 
ccaatcggg gtcaagtca cacctagca ccctttttg 
ccgggtcat cccgtcgtc aaccccttg ggcgaagac 
tccccaact tatggtgtt gtaagtgcc gcacctttc 
ggttcctct gccattctc cccatttcg tgggcttat 
atcaaaatg atgggcgct cgaggtttg aggaaagcc 
acccaagtc gcgatacta aacgccaat tacatgtcg 
aaagtatta gaacaacac tacaccact ttgtttaag 
agcccaacg tcggatttg gtcgctcac gagtttatt 
attgacaca agagagttt aaaaagact gccaatatc 
gaagctgca gatattgcg aaaagactg atggattat 
gggttccat gcgcccacc atgtcctgg cctgtggct 
gggactttg atgattgaa ccgacggaa tcggaggat 
aagcaggag ttggaccgg ttttgtgaa tcgttgatt 
tcgattaga caggagatt aaggatatt gaggacggg 
gttatggat aaaagggtg aatccgttg aagatggcg 
ccgcatacg caagaacag gttattaac agtgcttgg 
gagaggcca tatacgagg gaacaggcg gcgtttccg 
gctccgttt gtgaggcca gaagttaaa gtttggccg 
acggttgca aggattgat gatatttat ggcgataag 
catctcgtg tgtacgtgc ccccccatt ttggacgaa 
tacaattac taa
    mRNA sequence
    • >TC010496 RA
      ttatcatcc aagcaatca ttctgtaac agatgctat 
tcaatatgc aaacacgtc catttatca cacaattac 
tccacaatt tacataaaa attatcttg ctcttatca 
tatttttgc gacccctga ccccatcca tttatattg 
acaatgtga agtcgaaaa taattagtt cagtcgaga 
tcgattaat taactaaca agatgtaca aaattgtag 
tccgaaccc gcccttttt tccccaaac taaaaaatt 
tgcgatttc taagtcccg gtgctccaa aacccgacg 
gtcctttat ttccacaca aagatgaat tccctagtc 
gacacatcg gtcctaggg acagcgaca ttatccaaa 
tgttggact cgctcggtt ttaagagtt tggacgagt 
tgacggaca aggccgttc cacaaaaca ttcgcttga 
atcgacaat tggacattg aagagcccg taggcgagt 
acgagctga taaatcgaa tacgaaaaa tagcggaac 
gaaatcaaa tttggaggt catacattg gcatggggt 
accacaatt gttgcgtac cgcatacca tcatgcgaa 
atatttttg aaaatccag gctggacca ctcagtata 
ctccttatc agccggagg tgtcgcaag ggagactcg 
aagggttgc tcaactacc agacaatgg tctctgact 
tgacgggtt tggatgtgg ctaatgcct cacttttgg 
acgaaggga cagcagcag ctgaagccc tttcgcttt 
gttatcgac ataataaga ggcgaaaga tgcttatgt 
ctgataaac ttcacccac aaacaattt cagtcgttg 
agacacgtt tatcgtctt tgggacttc aagtcgaag 
ttgtggacg ttttcgaag ctgatttct cgaacagag 
acgttgcag gagttcttt tccaatatc cagacactg 
aaggaaaca ttcttgatt ttagtacgg tcacagaag 
ctgcacatg aacacggta ctctagtgt gttgcgcta 
cagatctgc tcgcgttaa ctcttctcc gaccaccga 
gtgagtttg gtgctgata ttgctgttg gtacttcgc 
aacggttgg gggtaccat tgggttatg gagggcccc 
atgctgggt tttttgctt gtaatcagt ccttggtgc 
gacttatgc caggcagga tgattggag tgacacgag 
atgctgctg gtcgagatg gatatcgtt tggcattac 
agacacgtg aacaacaca tcagaagag ataaggcta 
caagtaata tttgtaccg ctcaggcgt tactagcga 
atatgtcgg ccatgtttg cggtgtacc atggaccac 
aagggctta aagatattg gtactaaaa tacacaaca 
tgacgttgg tattaagcc acggcttgc agcaagacg 
gtaacacct taacgaacg aactcttct tcgacactc 
tccgaatcg aaccaaaac tctccaccg aagagatcc 
aacgtcgag ccaacgcga aaaaaatca acctacgat 
atttcgacg acggctcag tcggagtct ccctcgacg 
aaacagtca ctttcgccg acattaacg acctgtttg 
aaatattcg gcagttcgc acaaaatcg aagacttgc 
tcaacaacc caatggtgc gcgaacact caatcacca 
aaggcgaat acaaaagaa cctccccgt ttctaacac 
acccagtat ttaactcac accattccg aaactagga 
tcgttcgct acatgaaaa tcttggaga ataaggaca 
tttctctgg tccattcga tgatcccgc ttggttctt 
gcacaatga aacttaact caacgacag agatgatgc 
cttgttcct tcaaacact ttactgata ttcatccgt 
ttgcgcctt tggatcaaa ctttggggt atcaccagt 
tgtttgcag agcttgaga aagatcttt gtgccatca 
ctgggtatg acaagatta gtttccagc cgaatagtg 
gcgcccaag gtgaatatg caggtctga gggccattc 
agtgctacc atgaagccc gaggtgata aaaatcggg 
acgtttgtt tgattcctg tgagtgccc atgggacga 
acccagcaa gtgcccaaa tggccggga tgcggattg 
aaccagtca gagtgaaac atgatggtt cgatcgatg 
tcgaagatc ttaaagcga aggccgaga agttcaaca 
gtcgtctct cgtgcatga tgatcacct acccatcaa 
ctaatggac tttttgagg aaacggtcg cagatgtgt 
gtgatatta tccataaaa acggtggtc aggtctacc 
tagatggtg ccaacatga acgcacaag tcggattgt 
gccgacctg gtgattacg gcggtgacg tctcccatc 
tgaacttgc acaaaacct tctgcatac cgcatggag 
gtggtggtc ctggtatgg gtccaatcg gggtcaagt 
cacacctag caccctttt tgccgggtc atcccgtcg 
tcaacccct tgggcgaag actccccaa cttatggtg 
ttgtaagtg ccgcacctt tcggttcct ctgccattc 
tccccattt cgtgggctt atatcaaaa tgatgggcg 
ctcgaggtt tgaggaaag ccacccaag tcgcgatac 
taaacgcca attacatgt cgaaagtat tagaacaac 
actacacca ctttgttta agagcccaa cgtcggatt 
tggtcgctc acgagttta ttattgaca caagagagt 
ttaaaaaga ctgccaata tcgaagctg cagatattg 
cgaaaagac tgatggatt atgggttcc atgcgccca 
ccatgtcct ggcctgtgg ctgggactt tgatgattg 
aaccgacgg aatcggagg ataagcagg agttggacc 
ggttttgtg aatcgttga tttcgatta gacaggaga 
ttaaggata ttgaggacg gggttatgg ataaaaggg 
tgaatccgt tgaagatgg cgccgcata cgcaagaac 
aggttatta acagtgctt gggagaggc catatacga 
gggaacagg cggcgtttc cggctccgt ttgtgaggc 
cagaagtta aagtttggc cgacggttg caaggattg 
atgatattt atggcgata agcatctcg tgtgtacgt 
gccccccca ttttggacg aatacaatt actaaatgt 
ttggatcag aatgtatta tttattgaa tcgacgcta 
caataattt ttaaggaaa ttggaattt tttctgaag 
ctgcgcaaa atcaaatct agtcatcat attcttatt 
tgcgattat tgtacacgt aacaatgaa ttcgagtca 
gactttgcc taattatgt acataatac ctaataata 
acaataata ttaacagaa atac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY