Status
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Gene information
General information
TC010496 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15582816..15592896
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010496
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04853

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010496 RA
      MYKIVVRTR PFFPKLKNL RFLSPGAPK PDGPLFPHK 
      DEFPSRHIG PRDSDIIQM LDSLGFKSL DELTDKAVP 
      QNIRLNRQL DIEEPVGEY ELINRIRKI AERNQIWRS 
      YIGMGYHNC CVPHTIMRN IFENPGWTT QYTPYQPEV 
      SQGRLEGLL NYQTMVSDL TGLDVANAS LLDEGTAAA 
      EALSLCYRH NKRRKMLMS DKLHPQTIS VVETRLSSL 
      GLQVEVVDV FEADFSNRD VAGVLFQYP DTEGNILDF 
      STVTEAAHE HGTLVCCAT DLLALTLLR PPSEFGADI 
      AVGTSQRLG VPLGYGGPH AGFFACNQS LVRLMPGRM 
      IGVTRDAAG RDGYRLALQ TREQHIRRD KATSNICTA 
      QALLANMSA MFAVYHGPQ GLKDIGTKI HNMTLVLSH 
      GLQQDGNTL TNELFFDTL RIEPKLSTE EIQRRANAK 
      KINLRYFDD GSVGVSLDE TVTFADIND LFEIFGSSH 
      KIEDLLNNP MVREHSITK GEYKRTSPF LTHPVFNSH 
      HSETRIVRY MKILENKDI SLVHSMIPL GSCTMKLNS 
      TTEMMPCSF KHFTDIHPF APLDQTLGY HQLFAELEK 
      DLCAITGYD KISFQPNSG AQGEYAGLR AIQCYHEAR 
      GDKNRDVCL IPVSAHGTN PASAQMAGM RIEPVRVKH 
      DGSIDVEDL KAKAEKFNS RLSCMMITY PSTNGLFEE 
      TVADVCDII HKNGGQVYL DGANMNAQV GLCRPGDYG 
      GDVSHLNLH KTFCIPHGG GGPGMGPIG VKSHLAPFL 
      PGHPVVNPL GEDSPTYGV VSAAPFGSS AILPISWAY 
      IKMMGARGL RKATQVAIL NANYMSKVL EQHYTTLFK 
      SPTSDLVAH EFIIDTREF KKTANIEAA DIAKRLMDY 
      GFHAPTMSW PVAGTLMIE PTESEDKQE LDRFCESLI 
      SIRQEIKDI EDGVMDKRV NPLKMAPHT QEQVINSAW 
      ERPYTREQA AFPAPFVRP EVKVWPTVA RIDDIYGDK 
      HLVCTCPPI LDEYNY
    CDS sequence
    • >TC010496 RA
      atgtacaaa attgtagtc cgaacccgc ccttttttc 
      cccaaacta aaaaatttg cgatttcta agtcccggt 
      gctccaaaa cccgacggt cctttattt ccacacaaa 
      gatgaattc cctagtcga cacatcggt cctagggac 
      agcgacatt atccaaatg ttggactcg ctcggtttt 
      aagagtttg gacgagttg acggacaag gccgttcca 
      caaaacatt cgcttgaat cgacaattg gacattgaa 
      gagcccgta ggcgagtac gagctgata aatcgaata 
      cgaaaaata gcggaacga aatcaaatt tggaggtca 
      tacattggc atggggtac cacaattgt tgcgtaccg 
      cataccatc atgcgaaat atttttgaa aatccaggc 
      tggaccact cagtatact ccttatcag ccggaggtg 
      tcgcaaggg agactcgaa gggttgctc aactaccag 
      acaatggtc tctgacttg acgggtttg gatgtggct 
      aatgcctca cttttggac gaagggaca gcagcagct 
      gaagccctt tcgctttgt tatcgacat aataagagg 
      cgaaagatg cttatgtct gataaactt cacccacaa 
      acaatttca gtcgttgag acacgttta tcgtctttg 
      ggacttcaa gtcgaagtt gtggacgtt ttcgaagct 
      gatttctcg aacagagac gttgcagga gttcttttc 
      caatatcca gacactgaa ggaaacatt cttgatttt 
      agtacggtc acagaagct gcacatgaa cacggtact 
      ctagtgtgt tgcgctaca gatctgctc gcgttaact 
      cttctccga ccaccgagt gagtttggt gctgatatt 
      gctgttggt acttcgcaa cggttgggg gtaccattg 
      ggttatgga gggccccat gctgggttt tttgcttgt 
      aatcagtcc ttggtgcga cttatgcca ggcaggatg 
      attggagtg acacgagat gctgctggt cgagatgga 
      tatcgtttg gcattacag acacgtgaa caacacatc 
      agaagagat aaggctaca agtaatatt tgtaccgct 
      caggcgtta ctagcgaat atgtcggcc atgtttgcg 
      gtgtaccat ggaccacaa gggcttaaa gatattggt 
      actaaaata cacaacatg acgttggta ttaagccac 
      ggcttgcag caagacggt aacacctta acgaacgaa 
      ctcttcttc gacactctc cgaatcgaa ccaaaactc 
      tccaccgaa gagatccaa cgtcgagcc aacgcgaaa 
      aaaatcaac ctacgatat ttcgacgac ggctcagtc 
      ggagtctcc ctcgacgaa acagtcact ttcgccgac 
      attaacgac ctgtttgaa atattcggc agttcgcac 
      aaaatcgaa gacttgctc aacaaccca atggtgcgc 
      gaacactca atcaccaaa ggcgaatac aaaagaacc 
      tccccgttt ctaacacac ccagtattt aactcacac 
      cattccgaa actaggatc gttcgctac atgaaaatc 
      ttggagaat aaggacatt tctctggtc cattcgatg 
      atcccgctt ggttcttgc acaatgaaa cttaactca 
      acgacagag atgatgcct tgttccttc aaacacttt 
      actgatatt catccgttt gcgcctttg gatcaaact 
      ttggggtat caccagttg tttgcagag cttgagaaa 
      gatctttgt gccatcact gggtatgac aagattagt 
      ttccagccg aatagtggc gcccaaggt gaatatgca 
      ggtctgagg gccattcag tgctaccat gaagcccga 
      ggtgataaa aatcgggac gtttgtttg attcctgtg 
      agtgcccat gggacgaac ccagcaagt gcccaaatg 
      gccgggatg cggattgaa ccagtcaga gtgaaacat 
      gatggttcg atcgatgtc gaagatctt aaagcgaag 
      gccgagaag ttcaacagt cgtctctcg tgcatgatg 
      atcacctac ccatcaact aatggactt tttgaggaa 
      acggtcgca gatgtgtgt gatattatc cataaaaac 
      ggtggtcag gtctaccta gatggtgcc aacatgaac 
      gcacaagtc ggattgtgc cgacctggt gattacggc 
      ggtgacgtc tcccatctg aacttgcac aaaaccttc 
      tgcataccg catggaggt ggtggtcct ggtatgggt 
      ccaatcggg gtcaagtca cacctagca ccctttttg 
      ccgggtcat cccgtcgtc aaccccttg ggcgaagac 
      tccccaact tatggtgtt gtaagtgcc gcacctttc 
      ggttcctct gccattctc cccatttcg tgggcttat 
      atcaaaatg atgggcgct cgaggtttg aggaaagcc 
      acccaagtc gcgatacta aacgccaat tacatgtcg 
      aaagtatta gaacaacac tacaccact ttgtttaag 
      agcccaacg tcggatttg gtcgctcac gagtttatt 
      attgacaca agagagttt aaaaagact gccaatatc 
      gaagctgca gatattgcg aaaagactg atggattat 
      gggttccat gcgcccacc atgtcctgg cctgtggct 
      gggactttg atgattgaa ccgacggaa tcggaggat 
      aagcaggag ttggaccgg ttttgtgaa tcgttgatt 
      tcgattaga caggagatt aaggatatt gaggacggg 
      gttatggat aaaagggtg aatccgttg aagatggcg 
      ccgcatacg caagaacag gttattaac agtgcttgg 
      gagaggcca tatacgagg gaacaggcg gcgtttccg 
      gctccgttt gtgaggcca gaagttaaa gtttggccg 
      acggttgca aggattgat gatatttat ggcgataag 
      catctcgtg tgtacgtgc ccccccatt ttggacgaa 
      tacaattac taa
    mRNA sequence
    • >TC010496 RA
      ttatcatcc aagcaatca ttctgtaac agatgctat 
      tcaatatgc aaacacgtc catttatca cacaattac 
      tccacaatt tacataaaa attatcttg ctcttatca 
      tatttttgc gacccctga ccccatcca tttatattg 
      acaatgtga agtcgaaaa taattagtt cagtcgaga 
      tcgattaat taactaaca agatgtaca aaattgtag 
      tccgaaccc gcccttttt tccccaaac taaaaaatt 
      tgcgatttc taagtcccg gtgctccaa aacccgacg 
      gtcctttat ttccacaca aagatgaat tccctagtc 
      gacacatcg gtcctaggg acagcgaca ttatccaaa 
      tgttggact cgctcggtt ttaagagtt tggacgagt 
      tgacggaca aggccgttc cacaaaaca ttcgcttga 
      atcgacaat tggacattg aagagcccg taggcgagt 
      acgagctga taaatcgaa tacgaaaaa tagcggaac 
      gaaatcaaa tttggaggt catacattg gcatggggt 
      accacaatt gttgcgtac cgcatacca tcatgcgaa 
      atatttttg aaaatccag gctggacca ctcagtata 
      ctccttatc agccggagg tgtcgcaag ggagactcg 
      aagggttgc tcaactacc agacaatgg tctctgact 
      tgacgggtt tggatgtgg ctaatgcct cacttttgg 
      acgaaggga cagcagcag ctgaagccc tttcgcttt 
      gttatcgac ataataaga ggcgaaaga tgcttatgt 
      ctgataaac ttcacccac aaacaattt cagtcgttg 
      agacacgtt tatcgtctt tgggacttc aagtcgaag 
      ttgtggacg ttttcgaag ctgatttct cgaacagag 
      acgttgcag gagttcttt tccaatatc cagacactg 
      aaggaaaca ttcttgatt ttagtacgg tcacagaag 
      ctgcacatg aacacggta ctctagtgt gttgcgcta 
      cagatctgc tcgcgttaa ctcttctcc gaccaccga 
      gtgagtttg gtgctgata ttgctgttg gtacttcgc 
      aacggttgg gggtaccat tgggttatg gagggcccc 
      atgctgggt tttttgctt gtaatcagt ccttggtgc 
      gacttatgc caggcagga tgattggag tgacacgag 
      atgctgctg gtcgagatg gatatcgtt tggcattac 
      agacacgtg aacaacaca tcagaagag ataaggcta 
      caagtaata tttgtaccg ctcaggcgt tactagcga 
      atatgtcgg ccatgtttg cggtgtacc atggaccac 
      aagggctta aagatattg gtactaaaa tacacaaca 
      tgacgttgg tattaagcc acggcttgc agcaagacg 
      gtaacacct taacgaacg aactcttct tcgacactc 
      tccgaatcg aaccaaaac tctccaccg aagagatcc 
      aacgtcgag ccaacgcga aaaaaatca acctacgat 
      atttcgacg acggctcag tcggagtct ccctcgacg 
      aaacagtca ctttcgccg acattaacg acctgtttg 
      aaatattcg gcagttcgc acaaaatcg aagacttgc 
      tcaacaacc caatggtgc gcgaacact caatcacca 
      aaggcgaat acaaaagaa cctccccgt ttctaacac 
      acccagtat ttaactcac accattccg aaactagga 
      tcgttcgct acatgaaaa tcttggaga ataaggaca 
      tttctctgg tccattcga tgatcccgc ttggttctt 
      gcacaatga aacttaact caacgacag agatgatgc 
      cttgttcct tcaaacact ttactgata ttcatccgt 
      ttgcgcctt tggatcaaa ctttggggt atcaccagt 
      tgtttgcag agcttgaga aagatcttt gtgccatca 
      ctgggtatg acaagatta gtttccagc cgaatagtg 
      gcgcccaag gtgaatatg caggtctga gggccattc 
      agtgctacc atgaagccc gaggtgata aaaatcggg 
      acgtttgtt tgattcctg tgagtgccc atgggacga 
      acccagcaa gtgcccaaa tggccggga tgcggattg 
      aaccagtca gagtgaaac atgatggtt cgatcgatg 
      tcgaagatc ttaaagcga aggccgaga agttcaaca 
      gtcgtctct cgtgcatga tgatcacct acccatcaa 
      ctaatggac tttttgagg aaacggtcg cagatgtgt 
      gtgatatta tccataaaa acggtggtc aggtctacc 
      tagatggtg ccaacatga acgcacaag tcggattgt 
      gccgacctg gtgattacg gcggtgacg tctcccatc 
      tgaacttgc acaaaacct tctgcatac cgcatggag 
      gtggtggtc ctggtatgg gtccaatcg gggtcaagt 
      cacacctag caccctttt tgccgggtc atcccgtcg 
      tcaacccct tgggcgaag actccccaa cttatggtg 
      ttgtaagtg ccgcacctt tcggttcct ctgccattc 
      tccccattt cgtgggctt atatcaaaa tgatgggcg 
      ctcgaggtt tgaggaaag ccacccaag tcgcgatac 
      taaacgcca attacatgt cgaaagtat tagaacaac 
      actacacca ctttgttta agagcccaa cgtcggatt 
      tggtcgctc acgagttta ttattgaca caagagagt 
      ttaaaaaga ctgccaata tcgaagctg cagatattg 
      cgaaaagac tgatggatt atgggttcc atgcgccca 
      ccatgtcct ggcctgtgg ctgggactt tgatgattg 
      aaccgacgg aatcggagg ataagcagg agttggacc 
      ggttttgtg aatcgttga tttcgatta gacaggaga 
      ttaaggata ttgaggacg gggttatgg ataaaaggg 
      tgaatccgt tgaagatgg cgccgcata cgcaagaac 
      aggttatta acagtgctt gggagaggc catatacga 
      gggaacagg cggcgtttc cggctccgt ttgtgaggc 
      cagaagtta aagtttggc cgacggttg caaggattg 
      atgatattt atggcgata agcatctcg tgtgtacgt 
      gccccccca ttttggacg aatacaatt actaaatgt 
      ttggatcag aatgtatta tttattgaa tcgacgcta 
      caataattt ttaaggaaa ttggaattt tttctgaag 
      ctgcgcaaa atcaaatct agtcatcat attcttatt 
      tgcgattat tgtacacgt aacaatgaa ttcgagtca 
      gactttgcc taattatgt acataatac ctaataata 
      acaataata ttaacagaa atac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY