Status
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Gene information
General information
TC010493 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 15655122..15656857
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010493
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
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      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04852

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
      Days post injection


      larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
      Days post injection


      larva lethality late (L7) - Number of animals showing the phenotype: 6 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010493 RA
        MKKRKHSRV SESAELSEA DDAEIIKVN IQTKSSSLS 
        PNPSDLDEA PPSPPPPKS VEKATPSPK KSPLKNFIS 
        QHPELEKVA LDDVVSEDD DICVLEVPS TLDSDCFIG 
        KLINFDKKT KLKISGKKY IIEPNFEDV RSINLISRG 
        IKVVKPVAN LKLREYHKN KPDDNIVNV DKCKPTLPE 
        KIKVRHPLF GVEYKKKIE LSEEVQRKL DNSIEKALQ 
        KIKKKKKKN KHVEKEDSQ VKNNIKPIK QEAFTSGDD 
        EQDGPFFEA LRSHCEKKN KSKHRIKAE VDDQFYESS 
        LDEKKKKTH QVSPEYKEE KDEKKKKRK SSFNEGSSD 
        EKRRKHSSH TFESNNSFE EKKHGNNLV NSTVIPGLH 
        LPLFDFDVS GIINKDSEV NNDDLEENE QSVKSKKKK 
        KTVKVEPET DHSVSSEGT SPKKKKKKI KEEKVDEQT 
        NSLIFDLLN KVKSEIDSP AKHKKKKKA DG
      CDS sequence
      • >TC010493 RA
        atgaaaaaa cgcaaacat tctagagtt tcagaaagt 
        gcagaatta tcagaagcc gatgatgca gaaatcatc 
        aaagtaaat atccaaacg aaatcaagc tcactttca 
        cctaacccc tcagacttg gacgaagct ccaccatca 
        cccccgcca ccaaaatca gtagaaaaa gcgaccccg 
        agtccgaaa aaaagccct ctgaaaaac tttatttcg 
        caacaccct gagttagag aaggtcgca ctagacgat 
        gtggtatca gaagatgac gatatttgc gtactggaa 
        gttcctagc actttagat tcggattgt ttcatcggg 
        aaattaatt aatttcgac aaaaaaact aaactcaaa 
        atcagtggt aaaaaatac ataattgag ccaaatttt 
        gaagacgtt agatcgatt aatttaatc agtcgtggc 
        attaaagta gttaaacct gtcgcgaat ttaaaactt 
        cgtgaatac cacaaaaat aaacctgac gacaatatt 
        gtgaatgtg gataaatgt aaaccaacg cttcctgaa 
        aaaattaaa gtaagacac ccacttttt ggggttgag 
        tacaaaaag aagatcgag ttgagtgaa gaagtacaa 
        aggaaattg gacaactcg atcgagaag gctttgcaa 
        aaaattaag aaaaagaag aaaaagaat aaacacgtg 
        gaaaaggaa gacagtcaa gtgaagaat aacataaaa 
        ccaattaag caagaagca ttcacttcg ggggatgat 
        gaacaagat ggacctttc tttgaggct ttgagatca 
        cactgtgaa aagaaaaac aaatctaaa caccgaata 
        aaagctgaa gtggacgat caattttac gaatcttct 
        ctggacgag aaaaagaag aaaacgcat caagtatcc 
        cccgaatat aaagaagag aaagatgag aagaagaag 
        aaacgtaag agtagcttt aatgaggga tcttctgat 
        gaaaaacga agaaaacac tcaagtcac acattcgaa 
        agtaacaac agttttgaa gagaagaaa cacggaaac 
        aatttggtc aacagtacg gtaatccct ggtttacat 
        ttaccactt ttcgatttt gatgttagt ggaattatt 
        aataaagat tcggaagtg aacaacgat gatttggaa 
        gagaatgag caaagcgta aagagcaag aagaagaag 
        aaaacggtg aaagtggaa cccgagaca gatcattct 
        gtgtcaagt gagggaacg tcgccaaaa aagaagaag 
        aaaaagatt aaggaagaa aaagtggat gagcaaact 
        aattcgtta atttttgac cttttgaat aaagtgaag 
        agcgagata gacagtccg gcgaagcac aagaagaag 
        aaaaaagca gatgggtaa
      mRNA sequence
      • >TC010493 RA
        tccaccgcg cgtttctta attatgaca taacctcaa 
        ttttgaccg tttttataa aaattaata tttacattt 
        aaaatgaaa aaacgcaaa cattctaga gtttcagaa 
        agtgcagaa ttatcagaa gccgatgat gcagaaatc 
        atcaaagta aatatccaa acgaaatca agctcactt 
        tcacctaac ccctcagac ttggacgaa gctccacca 
        tcacccccg ccaccaaaa tcagtagaa aaagcgacc 
        ccgagtccg aaaaaaagc cctctgaaa aactttatt 
        tcgcaacac cctgagtta gagaaggtc gcactagac 
        gatgtggta tcagaagat gacgatatt tgcgtactg 
        gaagttcct agcacttta gattcggat tgtttcatc 
        gggaaatta attaatttc gacaaaaaa actaaactc 
        aaaatcagt ggtaaaaaa tacataatt gagccaaat 
        tttgaagac gttagatcg attaattta atcagtcgt 
        ggcattaaa gtagttaaa cctgtcgcg aatttaaaa 
        cttcgtgaa taccacaaa aataaacct gacgacaat 
        attgtgaat gtggataaa tgtaaacca acgcttcct 
        gaaaaaatt aaagtaaga cacccactt tttggggtt 
        gagtacaaa aagaagatc gagttgagt gaagaagta 
        caaaggaaa ttggacaac tcgatcgag aaggctttg 
        caaaaaatt aagaaaaag aagaaaaag aataaacac 
        gtggaaaag gaagacagt caagtgaag aataacata 
        aaaccaatt aagcaagaa gcattcact tcgggggat 
        gatgaacaa gatggacct ttctttgag gctttgaga 
        tcacactgt gaaaagaaa aacaaatct aaacaccga 
        ataaaagct gaagtggac gatcaattt tacgaatct 
        tctctggac gagaaaaag aagaaaacg catcaagta 
        tcccccgaa tataaagaa gagaaagat gagaagaag 
        aagaaacgt aagagtagc tttaatgag ggatcttct 
        gatgaaaaa cgaagaaaa cactcaagt cacacattc 
        gaaagtaac aacagtttt gaagagaag aaacacgga 
        aacaatttg gtcaacagt acggtaatc cctggttta 
        catttacca cttttcgat tttgatgtt agtggaatt 
        attaataaa gattcggaa gtgaacaac gatgatttg 
        gaagagaat gagcaaagc gtaaagagc aagaagaag 
        aagaaaacg gtgaaagtg gaacccgag acagatcat 
        tctgtgtca agtgaggga acgtcgcca aaaaagaag 
        aagaaaaag attaaggaa gaaaaagtg gatgagcaa 
        actaattcg ttaattttt gaccttttg aataaagtg 
        aagagcgag atagacagt ccggcgaag cacaagaag 
        aagaaaaaa gcagatggg taattaact gtaaacatg 
        gcgactttt ttattttat caaatgatt taatgaaat 
        atattttct tttaagtca cagtttttt accttcttt 
        cttgtgcgt caaattgtt taaacctga ctgaaaagt 
        tactaaata gttgggatc aaaaaacat tttttatat 
        ttgtcggaa aattccgtc aaaattata ataaacaat 
        agaaaattt g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY