General information | |||
TC010493 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 15655122..15656857 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC010493 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04852 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 6 (19 dpi)
- >TC010493 RA
MKKRKHSRV SESAELSEA DDAEIIKVN IQTKSSSLS PNPSDLDEA PPSPPPPKS VEKATPSPK KSPLKNFIS QHPELEKVA LDDVVSEDD DICVLEVPS TLDSDCFIG KLINFDKKT KLKISGKKY IIEPNFEDV RSINLISRG IKVVKPVAN LKLREYHKN KPDDNIVNV DKCKPTLPE KIKVRHPLF GVEYKKKIE LSEEVQRKL DNSIEKALQ KIKKKKKKN KHVEKEDSQ VKNNIKPIK QEAFTSGDD EQDGPFFEA LRSHCEKKN KSKHRIKAE VDDQFYESS LDEKKKKTH QVSPEYKEE KDEKKKKRK SSFNEGSSD EKRRKHSSH TFESNNSFE EKKHGNNLV NSTVIPGLH LPLFDFDVS GIINKDSEV NNDDLEENE QSVKSKKKK KTVKVEPET DHSVSSEGT SPKKKKKKI KEEKVDEQT NSLIFDLLN KVKSEIDSP AKHKKKKKA DG
- >TC010493 RA
atgaaaaaa cgcaaacat tctagagtt tcagaaagt gcagaatta tcagaagcc gatgatgca gaaatcatc aaagtaaat atccaaacg aaatcaagc tcactttca cctaacccc tcagacttg gacgaagct ccaccatca cccccgcca ccaaaatca gtagaaaaa gcgaccccg agtccgaaa aaaagccct ctgaaaaac tttatttcg caacaccct gagttagag aaggtcgca ctagacgat gtggtatca gaagatgac gatatttgc gtactggaa gttcctagc actttagat tcggattgt ttcatcggg aaattaatt aatttcgac aaaaaaact aaactcaaa atcagtggt aaaaaatac ataattgag ccaaatttt gaagacgtt agatcgatt aatttaatc agtcgtggc attaaagta gttaaacct gtcgcgaat ttaaaactt cgtgaatac cacaaaaat aaacctgac gacaatatt gtgaatgtg gataaatgt aaaccaacg cttcctgaa aaaattaaa gtaagacac ccacttttt ggggttgag tacaaaaag aagatcgag ttgagtgaa gaagtacaa aggaaattg gacaactcg atcgagaag gctttgcaa aaaattaag aaaaagaag aaaaagaat aaacacgtg gaaaaggaa gacagtcaa gtgaagaat aacataaaa ccaattaag caagaagca ttcacttcg ggggatgat gaacaagat ggacctttc tttgaggct ttgagatca cactgtgaa aagaaaaac aaatctaaa caccgaata aaagctgaa gtggacgat caattttac gaatcttct ctggacgag aaaaagaag aaaacgcat caagtatcc cccgaatat aaagaagag aaagatgag aagaagaag aaacgtaag agtagcttt aatgaggga tcttctgat gaaaaacga agaaaacac tcaagtcac acattcgaa agtaacaac agttttgaa gagaagaaa cacggaaac aatttggtc aacagtacg gtaatccct ggtttacat ttaccactt ttcgatttt gatgttagt ggaattatt aataaagat tcggaagtg aacaacgat gatttggaa gagaatgag caaagcgta aagagcaag aagaagaag aaaacggtg aaagtggaa cccgagaca gatcattct gtgtcaagt gagggaacg tcgccaaaa aagaagaag aaaaagatt aaggaagaa aaagtggat gagcaaact aattcgtta atttttgac cttttgaat aaagtgaag agcgagata gacagtccg gcgaagcac aagaagaag aaaaaagca gatgggtaa
- >TC010493 RA
tccaccgcg cgtttctta attatgaca taacctcaa ttttgaccg tttttataa aaattaata tttacattt aaaatgaaa aaacgcaaa cattctaga gtttcagaa agtgcagaa ttatcagaa gccgatgat gcagaaatc atcaaagta aatatccaa acgaaatca agctcactt tcacctaac ccctcagac ttggacgaa gctccacca tcacccccg ccaccaaaa tcagtagaa aaagcgacc ccgagtccg aaaaaaagc cctctgaaa aactttatt tcgcaacac cctgagtta gagaaggtc gcactagac gatgtggta tcagaagat gacgatatt tgcgtactg gaagttcct agcacttta gattcggat tgtttcatc gggaaatta attaatttc gacaaaaaa actaaactc aaaatcagt ggtaaaaaa tacataatt gagccaaat tttgaagac gttagatcg attaattta atcagtcgt ggcattaaa gtagttaaa cctgtcgcg aatttaaaa cttcgtgaa taccacaaa aataaacct gacgacaat attgtgaat gtggataaa tgtaaacca acgcttcct gaaaaaatt aaagtaaga cacccactt tttggggtt gagtacaaa aagaagatc gagttgagt gaagaagta caaaggaaa ttggacaac tcgatcgag aaggctttg caaaaaatt aagaaaaag aagaaaaag aataaacac gtggaaaag gaagacagt caagtgaag aataacata aaaccaatt aagcaagaa gcattcact tcgggggat gatgaacaa gatggacct ttctttgag gctttgaga tcacactgt gaaaagaaa aacaaatct aaacaccga ataaaagct gaagtggac gatcaattt tacgaatct tctctggac gagaaaaag aagaaaacg catcaagta tcccccgaa tataaagaa gagaaagat gagaagaag aagaaacgt aagagtagc tttaatgag ggatcttct gatgaaaaa cgaagaaaa cactcaagt cacacattc gaaagtaac aacagtttt gaagagaag aaacacgga aacaatttg gtcaacagt acggtaatc cctggttta catttacca cttttcgat tttgatgtt agtggaatt attaataaa gattcggaa gtgaacaac gatgatttg gaagagaat gagcaaagc gtaaagagc aagaagaag aagaaaacg gtgaaagtg gaacccgag acagatcat tctgtgtca agtgaggga acgtcgcca aaaaagaag aagaaaaag attaaggaa gaaaaagtg gatgagcaa actaattcg ttaattttt gaccttttg aataaagtg aagagcgag atagacagt ccggcgaag cacaagaag aagaaaaaa gcagatggg taattaact gtaaacatg gcgactttt ttattttat caaatgatt taatgaaat atattttct tttaagtca cagtttttt accttcttt cttgtgcgt caaattgtt taaacctga ctgaaaagt tactaaata gttgggatc aaaaaacat tttttatat ttgtcggaa aattccgtc aaaattata ataaacaat agaaaattt g
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
