| General information | |||
| TC010475 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451027.1: 221753..235928 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010475 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04846 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
- >TC010475 RA
MGKQNSKLK PEVLEDLKQ NTEFSDAEI QEWYKGFLK DCPSGHLSV DEFKKIYGN FFPYGDASK FAEHVFRTF DANGDGTID FREFLCALS VTSRGKLEQ KLKWAFSMY DLDGNGYIS RQEMLEIVT AIYKMVGSV MKMPEDEST PEKRTDKIF RQMDRNKDG KLSLEEFIE GAKSDPSIV RLLQCDPQA Q
- >TC010475 RA
atggggaag cagaatagt aaactgaaa ccggaagtt ttggaagat cttaaacag aacacagaa ttctcagac gctgagatt caagaatgg tacaaagga ttcctcaag gactgccct agcggccac ctttccgtc gacgaattt aaaaagatc tacggtaac ttttttcct tacggtgat gcgagcaag tttgcagaa catgttttt cgtacattt gatgcgaac ggagatggc accatcgat tttcgggag tttctgtgc gcgctgtcc gtcacctct cgaggaaaa ctagaacaa aagctcaaa tgggccttt tcaatgtac gacctagac ggtaatggt tatatatca cggcaagag atgctcgaa atagtcacg gcaatctac aagatggtg gggtctgtg atgaagatg cctgaagac gagagtacg cccgagaaa cgtaccgac aaaatcttc cgccagatg gaccgaaac aaggacggc aaactaagc ctcgaggag ttcatcgag ggagcaaaa agcgatcct tccatcgta cgactcctc caatgcgac ccccaagcg cagtag
- >TC010475 RA
catttaatg aaaaaaatc cgttaaaaa acaatcata aataagcaa aagttataa taatgtttc caattatta ttacaagta aaattttgt attaaaaag tgagtactt ttcaccacg caatacata actgtttgc tatgaaata aatcacaca atattttaa gatttttta aatttaagt gataaaaaa acaaactgt cagtggtca gtggtcagg atgtagcct tttcttttg aattagact gaaggtgta acaaaaaag ttaaaaact tacaaacag caaataaaa aaagaagag atgttccaa aggccattt attaagaag tttggaatt ttttacgta tgtcaaaaa ttttaagtg ataaaaaaa aataagtga gaggtttat cgagagcaa accaaaaaa atattctgg acaaagaaa aaactaatt gtaagtgat cagggtgaa gcttttttt tttaattag attgaaggt ggaacaaaa aagttaaga cttacaaca gctaactta aaaaaaaag aagagatgc tccaaaggc catttacta aaaaatttt gaattttta acattcatc aaaaagata tcgaaaata tgtaaattg ttataattc taataataa attaatata agaattaca atgatttgc taattacat aattacaaa tactaccag aaatttaaa ggaaaaaaa ttgaatcaa cataaaaca gagtgttta aagattttc gaatttttc ccaataaaa aagtattcc gggtgataa aaaaattat tgatattgg catcaaaaa taaaaaaaa attcgtact caattagaa aacgtttct gctaaatga ataaagaaa aacaatagt taagattga acaatgttt cgctttttc tagattgac tgaagaggt aactaaaaa agttaagaa acaaaaata attagtttt ctgttgata attgcagta tttttcgat ataagtatt tactaaaaa tacagggtg tttattttt gtttaaaaa tgttaaaaa taaaacttc tactaatag taaagtaat aaaaaaagc gaagccact attgtatct actccatta aataagtat ttttccaaa gagttttgc cattcactt ttgagcgct ttaaaacag tttgctata aaatctaaa aggaaaaaa acacatttt ttgccatac aaatgagtt tttctttat aattttcaa tttttacgg agaaatttg acaagagtg agattttgt agcatcata attagcaaa acttttcca tcaactcaa caaagaaaa aattaatta cgtgtggct ttttgcgac tggcttaag aaataatca aacaagtta aaaacctta acaagagga aatttgaaa agataaaga atgagctaa caaaactgc tggaaacgt aataaaaaa aacgcaaaa gtttaaaag ttcgaatag ttttctcta gataattgc agtacttcg tataaacgg tatatttaa aatacaggg ctcttttaa atgactttg gtgttcgtc gtatttttt attttttgg tgtttttct aaataatta acatggtgc tgaatcgag tgattcttt tctgtttta taattattc acttcaaga aaaataatt cttgaatga aagtacgct aataacgaa acaccaaat tttatactt ttttttagt cttcgatgt tttagatag attacataa attggctta tttgacggt taagtaacc ggttaaaca ccacaaaca caagcatgg ttttcagct aagtttgca aaaaaatca aaggcagac ataaacata aaaactcaa acatgaaaa ccaagctgt acttccttt ggggactgc gataaaatg aaaaacctg aagtaaaca tttccagta tcagtgtta aaaaactaa gattttatc gtagaataa tgaaatttt ctactgaag atgtatcac aatttgagc tcgcgcaaa ctattttca tgggaaatt tttttgttt tcaaaatag cacaatttt acactgccc tgttgaaaa gatcttgtt tagctacat gctgcctac gttttagtt gatatcaaa ataattaaa aaaaaacta atatcaaag taattaaag aaagggtat tttccatcc atggaacgt cttaacttt tgactgatt aaaagagag ttgggtata ttaattttt aattaacca actttaagt taattggtt ggaagtttc agtgcatat caaattgaa attggggac tgaaccgtt tatatcatt ttcgaagaa ttttcagaa acctctagt cgttgtaat aaacaatca aattgaaat tgcgtagcg taatgttgt aggtagaat aagaaattc acttgtctt gctctaact gtaaaggca cactagtgt atgaaactt tatcagctc ggatctatt ccatttctg ttcaccatt cctctcgtc aagtgcttg cacatttga gggcaaaca gctgagaat cttccaatg taaccaaat gtgtgagtg catttgtaa aaagttgca agtttgttc attccattc gctaggtta ccagtgctt tattttcta gttggtgtt tgtgagagc taatttttc aatgctgga aaattagct cgtgtcgca gttcgcata ggaaattgt ccttctagt tgataacta actaaaaat gcactctgt tttttgtgt ttttcgctc gattctctt acttcatgt tgaaacaac gtatcatca tggcataag taacaaatg tgatacatc atttagtgc atatttgtg aaagtgtta gttgattat ctattaatt aatattttt ctacttgca catattgta acataggca taacagaca tattactct agatgttta acgtaagtt tgtaacgca tgcaagggg ttgtggagc ctcttgtgt gcgacatat attatgtag atgggtatg ataacgaag ccaggagcc gctctctac gtaaaattg aattattaa cacttattg atggcagat attctttga atttttaac acaaaatat tatgacaat taaataata tacgtatta tttggcgaa tttgttgtt tttggtgtg tgaggagcg tgtgtgata cggtgtgat ttaagggag atgactcgg ttaactcgg tacagggag cggcagtga gctgttcgc aagaccatg gggaagcag aatagtaaa ctgaaaccg gaagttttg gaagatctt aaacagaac acagaattc tcagacgct gagattcaa gaatggtac aaaggattc ctcaaggac tgccctagc ggccacctt tccgtcgac gaatttaaa aagatctac ggtaacttt tttccttac ggtgatgcg agcaagttt gcagaacat gtttttcgt acatttgat gcgaacgga gatggcacc atcgatttt cgggagttt ctgtgcgcg ctgtccgtc acctctcga ggaaaacta gaacaaaag ctcaaatgg gccttttca atgtacgac ctagacggt aatggttat atatcacgg caagagatg ctcgaaata gtcacggca atctacaag atggtgggg tctgtgatg aagatgcct gaagacgag agtacgccc gagaaacgt accgacaaa atcttccgc cagatggac cgaaacaag gacggcaaa ctaagcctc gaggagttc atcgaggga gcaaaaagc gatccttcc atcgtacga ctcctccaa tgcgacccc caagcgcag tagcctaag accgcaaag ccggtcacc agagactct ttatcttaa gtttttatc aaccactct tgtgtgatc gcggggttt ccgcgtcga catcccggt tatataata actagagtt agaaaaaga tggatgtct gtctggatt cgtaagtaa aacatcacg ttgttgtgt ttccattta accgcggat tgttatgct caccctcgc atagatttt agtgacaga atatttaat cctaattta atttagaaa taactttaa gcagaacta tctacctac ttatattga atttcagtg gcgatctat aacttataa tattgcaat tgatgatat ctcaagtac tctactatc actatccaa atatatcaa ttgaatgtt actgttttc aaagttttt atgtgattc gagaaacgt tgaatgtag atggtatat tttattgta atattctaa tgttatctc gatggtaat tggtgctta actgtcggg cggactcgc gaccgaaat tagtttaga atttcatgc cgaaacaag ctcattgct gcgattttt ctaaattta aaatgtatc tcaaaaact aatattcaa atcagtgtt tgctgttaa atactggtt gagttaatt ttttcggaa tatttctac aattcctta taattttat aattaaaat tgattaatt ttaaaaaat tattattta tgtcattct aaaacaagg tgcttaaat gttgtctgt tccaaaaat atgataaac aatgccgtt agtcacaaa aacaaaata aataaaaag agttttaat ctaaacata tctgaaaat ttgcatttg tgttgtttt tttctattt attcagtct ttaattgaa ggttcattg gtgattcaa taatcacaa aaataagta aaaaacctt gtctaaaat ggagatttt aaacgaaat tttctcttt ttgttacat gggatactt ttttttggt tatttcttc gtttttttt tttttttaa atactttgt caaagtgtc aaaaatctc ggaattaat tcgaaaatg atattactt tgtttgtta cacttgaat ttagttggt ttttgatgg caatttccc caaaaaaaa attggagac acaaaattt ggtagaaac ttctaacgt ctaacttac cttctcagt tttttgtga aattttgtt aaattatac cgaaaaatc gcaaaattg gatcaaaaa tgatttctt agagtcaat cgaaatttt gtaaaaaaa taacactat tatagcttt tttcttgcg tttattagc acgtttgca agttagtaa catattttt tttgcaaaa tttcgtcac aaacaggcc aaaaatgta tcaaatcaa gccaaaaat gatgttaaa tttgccgtt ctacagtgt ttaggcatg tttttgaaa taaaaacta aagtttttt ctgggat
