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Gene information
General information
TC010475 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451027.1: 221753..235928
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010475
    iBeetle screen iB_04846

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010475 RA
      MGKQNSKLK PEVLEDLKQ NTEFSDAEI 
      QEWYKGFLK DCPSGHLSV DEFKKIYGN 
      FFPYGDASK FAEHVFRTF DANGDGTID 
      FREFLCALS VTSRGKLEQ KLKWAFSMY 
      DLDGNGYIS RQEMLEIVT AIYKMVGSV 
      MKMPEDEST PEKRTDKIF RQMDRNKDG 
      KLSLEEFIE GAKSDPSIV RLLQCDPQA 
      Q
    CDS sequence
    • >TC010475 RA
      atggggaag cagaatagt aaactgaaa ccggaagtt 
      ttggaagat cttaaacag aacacagaa ttctcagac 
      gctgagatt caagaatgg tacaaagga ttcctcaag 
      gactgccct agcggccac ctttccgtc gacgaattt 
      aaaaagatc tacggtaac ttttttcct tacggtgat 
      gcgagcaag tttgcagaa catgttttt cgtacattt 
      gatgcgaac ggagatggc accatcgat tttcgggag 
      tttctgtgc gcgctgtcc gtcacctct cgaggaaaa 
      ctagaacaa aagctcaaa tgggccttt tcaatgtac 
      gacctagac ggtaatggt tatatatca cggcaagag 
      atgctcgaa atagtcacg gcaatctac aagatggtg 
      gggtctgtg atgaagatg cctgaagac gagagtacg 
      cccgagaaa cgtaccgac aaaatcttc cgccagatg 
      gaccgaaac aaggacggc aaactaagc ctcgaggag 
      ttcatcgag ggagcaaaa agcgatcct tccatcgta 
      cgactcctc caatgcgac ccccaagcg cagtag
    mRNA sequence
    • >TC010475 RA
      catttaatg aaaaaaatc cgttaaaaa acaatcata 
      aataagcaa aagttataa taatgtttc caattatta 
      ttacaagta aaattttgt attaaaaag tgagtactt 
      ttcaccacg caatacata actgtttgc tatgaaata 
      aatcacaca atattttaa gatttttta aatttaagt 
      gataaaaaa acaaactgt cagtggtca gtggtcagg 
      atgtagcct tttcttttg aattagact gaaggtgta 
      acaaaaaag ttaaaaact tacaaacag caaataaaa 
      aaagaagag atgttccaa aggccattt attaagaag 
      tttggaatt ttttacgta tgtcaaaaa ttttaagtg 
      ataaaaaaa aataagtga gaggtttat cgagagcaa 
      accaaaaaa atattctgg acaaagaaa aaactaatt 
      gtaagtgat cagggtgaa gcttttttt tttaattag 
      attgaaggt ggaacaaaa aagttaaga cttacaaca 
      gctaactta aaaaaaaag aagagatgc tccaaaggc 
      catttacta aaaaatttt gaattttta acattcatc 
      aaaaagata tcgaaaata tgtaaattg ttataattc 
      taataataa attaatata agaattaca atgatttgc 
      taattacat aattacaaa tactaccag aaatttaaa 
      ggaaaaaaa ttgaatcaa cataaaaca gagtgttta 
      aagattttc gaatttttc ccaataaaa aagtattcc 
      gggtgataa aaaaattat tgatattgg catcaaaaa 
      taaaaaaaa attcgtact caattagaa aacgtttct 
      gctaaatga ataaagaaa aacaatagt taagattga 
      acaatgttt cgctttttc tagattgac tgaagaggt 
      aactaaaaa agttaagaa acaaaaata attagtttt 
      ctgttgata attgcagta tttttcgat ataagtatt 
      tactaaaaa tacagggtg tttattttt gtttaaaaa 
      tgttaaaaa taaaacttc tactaatag taaagtaat 
      aaaaaaagc gaagccact attgtatct actccatta 
      aataagtat ttttccaaa gagttttgc cattcactt 
      ttgagcgct ttaaaacag tttgctata aaatctaaa 
      aggaaaaaa acacatttt ttgccatac aaatgagtt 
      tttctttat aattttcaa tttttacgg agaaatttg 
      acaagagtg agattttgt agcatcata attagcaaa 
      acttttcca tcaactcaa caaagaaaa aattaatta 
      cgtgtggct ttttgcgac tggcttaag aaataatca 
      aacaagtta aaaacctta acaagagga aatttgaaa 
      agataaaga atgagctaa caaaactgc tggaaacgt 
      aataaaaaa aacgcaaaa gtttaaaag ttcgaatag 
      ttttctcta gataattgc agtacttcg tataaacgg 
      tatatttaa aatacaggg ctcttttaa atgactttg 
      gtgttcgtc gtatttttt attttttgg tgtttttct 
      aaataatta acatggtgc tgaatcgag tgattcttt 
      tctgtttta taattattc acttcaaga aaaataatt 
      cttgaatga aagtacgct aataacgaa acaccaaat 
      tttatactt ttttttagt cttcgatgt tttagatag 
      attacataa attggctta tttgacggt taagtaacc 
      ggttaaaca ccacaaaca caagcatgg ttttcagct 
      aagtttgca aaaaaatca aaggcagac ataaacata 
      aaaactcaa acatgaaaa ccaagctgt acttccttt 
      ggggactgc gataaaatg aaaaacctg aagtaaaca 
      tttccagta tcagtgtta aaaaactaa gattttatc 
      gtagaataa tgaaatttt ctactgaag atgtatcac 
      aatttgagc tcgcgcaaa ctattttca tgggaaatt 
      tttttgttt tcaaaatag cacaatttt acactgccc 
      tgttgaaaa gatcttgtt tagctacat gctgcctac 
      gttttagtt gatatcaaa ataattaaa aaaaaacta 
      atatcaaag taattaaag aaagggtat tttccatcc 
      atggaacgt cttaacttt tgactgatt aaaagagag 
      ttgggtata ttaattttt aattaacca actttaagt 
      taattggtt ggaagtttc agtgcatat caaattgaa 
      attggggac tgaaccgtt tatatcatt ttcgaagaa 
      ttttcagaa acctctagt cgttgtaat aaacaatca 
      aattgaaat tgcgtagcg taatgttgt aggtagaat 
      aagaaattc acttgtctt gctctaact gtaaaggca 
      cactagtgt atgaaactt tatcagctc ggatctatt 
      ccatttctg ttcaccatt cctctcgtc aagtgcttg 
      cacatttga gggcaaaca gctgagaat cttccaatg 
      taaccaaat gtgtgagtg catttgtaa aaagttgca 
      agtttgttc attccattc gctaggtta ccagtgctt 
      tattttcta gttggtgtt tgtgagagc taatttttc 
      aatgctgga aaattagct cgtgtcgca gttcgcata 
      ggaaattgt ccttctagt tgataacta actaaaaat 
      gcactctgt tttttgtgt ttttcgctc gattctctt 
      acttcatgt tgaaacaac gtatcatca tggcataag 
      taacaaatg tgatacatc atttagtgc atatttgtg 
      aaagtgtta gttgattat ctattaatt aatattttt 
      ctacttgca catattgta acataggca taacagaca 
      tattactct agatgttta acgtaagtt tgtaacgca 
      tgcaagggg ttgtggagc ctcttgtgt gcgacatat 
      attatgtag atgggtatg ataacgaag ccaggagcc 
      gctctctac gtaaaattg aattattaa cacttattg 
      atggcagat attctttga atttttaac acaaaatat 
      tatgacaat taaataata tacgtatta tttggcgaa 
      tttgttgtt tttggtgtg tgaggagcg tgtgtgata 
      cggtgtgat ttaagggag atgactcgg ttaactcgg 
      tacagggag cggcagtga gctgttcgc aagaccatg 
      gggaagcag aatagtaaa ctgaaaccg gaagttttg 
      gaagatctt aaacagaac acagaattc tcagacgct 
      gagattcaa gaatggtac aaaggattc ctcaaggac 
      tgccctagc ggccacctt tccgtcgac gaatttaaa 
      aagatctac ggtaacttt tttccttac ggtgatgcg 
      agcaagttt gcagaacat gtttttcgt acatttgat 
      gcgaacgga gatggcacc atcgatttt cgggagttt 
      ctgtgcgcg ctgtccgtc acctctcga ggaaaacta 
      gaacaaaag ctcaaatgg gccttttca atgtacgac 
      ctagacggt aatggttat atatcacgg caagagatg 
      ctcgaaata gtcacggca atctacaag atggtgggg 
      tctgtgatg aagatgcct gaagacgag agtacgccc 
      gagaaacgt accgacaaa atcttccgc cagatggac 
      cgaaacaag gacggcaaa ctaagcctc gaggagttc 
      atcgaggga gcaaaaagc gatccttcc atcgtacga 
      ctcctccaa tgcgacccc caagcgcag tagcctaag 
      accgcaaag ccggtcacc agagactct ttatcttaa 
      gtttttatc aaccactct tgtgtgatc gcggggttt 
      ccgcgtcga catcccggt tatataata actagagtt 
      agaaaaaga tggatgtct gtctggatt cgtaagtaa 
      aacatcacg ttgttgtgt ttccattta accgcggat 
      tgttatgct caccctcgc atagatttt agtgacaga 
      atatttaat cctaattta atttagaaa taactttaa 
      gcagaacta tctacctac ttatattga atttcagtg 
      gcgatctat aacttataa tattgcaat tgatgatat 
      ctcaagtac tctactatc actatccaa atatatcaa 
      ttgaatgtt actgttttc aaagttttt atgtgattc 
      gagaaacgt tgaatgtag atggtatat tttattgta 
      atattctaa tgttatctc gatggtaat tggtgctta 
      actgtcggg cggactcgc gaccgaaat tagtttaga 
      atttcatgc cgaaacaag ctcattgct gcgattttt 
      ctaaattta aaatgtatc tcaaaaact aatattcaa 
      atcagtgtt tgctgttaa atactggtt gagttaatt 
      ttttcggaa tatttctac aattcctta taattttat 
      aattaaaat tgattaatt ttaaaaaat tattattta 
      tgtcattct aaaacaagg tgcttaaat gttgtctgt 
      tccaaaaat atgataaac aatgccgtt agtcacaaa 
      aacaaaata aataaaaag agttttaat ctaaacata 
      tctgaaaat ttgcatttg tgttgtttt tttctattt 
      attcagtct ttaattgaa ggttcattg gtgattcaa 
      taatcacaa aaataagta aaaaacctt gtctaaaat 
      ggagatttt aaacgaaat tttctcttt ttgttacat 
      gggatactt ttttttggt tatttcttc gtttttttt 
      tttttttaa atactttgt caaagtgtc aaaaatctc 
      ggaattaat tcgaaaatg atattactt tgtttgtta 
      cacttgaat ttagttggt ttttgatgg caatttccc 
      caaaaaaaa attggagac acaaaattt ggtagaaac 
      ttctaacgt ctaacttac cttctcagt tttttgtga 
      aattttgtt aaattatac cgaaaaatc gcaaaattg 
      gatcaaaaa tgatttctt agagtcaat cgaaatttt 
      gtaaaaaaa taacactat tatagcttt tttcttgcg 
      tttattagc acgtttgca agttagtaa catattttt 
      tttgcaaaa tttcgtcac aaacaggcc aaaaatgta 
      tcaaatcaa gccaaaaat gatgttaaa tttgccgtt 
      ctacagtgt ttaggcatg tttttgaaa taaaaacta 
      aagtttttt ctgggat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY