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Gene information
General information
TC010475 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451027.1: 221753..235928
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010475
    iBeetle screen iB_04846

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010475 RA
      MGKQNSKLK PEVLEDLKQ NTEFSDAEI 
QEWYKGFLK DCPSGHLSV DEFKKIYGN 
FFPYGDASK FAEHVFRTF DANGDGTID 
FREFLCALS VTSRGKLEQ KLKWAFSMY 
DLDGNGYIS RQEMLEIVT AIYKMVGSV 
MKMPEDEST PEKRTDKIF RQMDRNKDG 
KLSLEEFIE GAKSDPSIV RLLQCDPQA 
Q
    CDS sequence
    • >TC010475 RA
      atggggaag cagaatagt aaactgaaa ccggaagtt 
ttggaagat cttaaacag aacacagaa ttctcagac 
gctgagatt caagaatgg tacaaagga ttcctcaag 
gactgccct agcggccac ctttccgtc gacgaattt 
aaaaagatc tacggtaac ttttttcct tacggtgat 
gcgagcaag tttgcagaa catgttttt cgtacattt 
gatgcgaac ggagatggc accatcgat tttcgggag 
tttctgtgc gcgctgtcc gtcacctct cgaggaaaa 
ctagaacaa aagctcaaa tgggccttt tcaatgtac 
gacctagac ggtaatggt tatatatca cggcaagag 
atgctcgaa atagtcacg gcaatctac aagatggtg 
gggtctgtg atgaagatg cctgaagac gagagtacg 
cccgagaaa cgtaccgac aaaatcttc cgccagatg 
gaccgaaac aaggacggc aaactaagc ctcgaggag 
ttcatcgag ggagcaaaa agcgatcct tccatcgta 
cgactcctc caatgcgac ccccaagcg cagtag
    mRNA sequence
    • >TC010475 RA
      catttaatg aaaaaaatc cgttaaaaa acaatcata 
aataagcaa aagttataa taatgtttc caattatta 
ttacaagta aaattttgt attaaaaag tgagtactt 
ttcaccacg caatacata actgtttgc tatgaaata 
aatcacaca atattttaa gatttttta aatttaagt 
gataaaaaa acaaactgt cagtggtca gtggtcagg 
atgtagcct tttcttttg aattagact gaaggtgta 
acaaaaaag ttaaaaact tacaaacag caaataaaa 
aaagaagag atgttccaa aggccattt attaagaag 
tttggaatt ttttacgta tgtcaaaaa ttttaagtg 
ataaaaaaa aataagtga gaggtttat cgagagcaa 
accaaaaaa atattctgg acaaagaaa aaactaatt 
gtaagtgat cagggtgaa gcttttttt tttaattag 
attgaaggt ggaacaaaa aagttaaga cttacaaca 
gctaactta aaaaaaaag aagagatgc tccaaaggc 
catttacta aaaaatttt gaattttta acattcatc 
aaaaagata tcgaaaata tgtaaattg ttataattc 
taataataa attaatata agaattaca atgatttgc 
taattacat aattacaaa tactaccag aaatttaaa 
ggaaaaaaa ttgaatcaa cataaaaca gagtgttta 
aagattttc gaatttttc ccaataaaa aagtattcc 
gggtgataa aaaaattat tgatattgg catcaaaaa 
taaaaaaaa attcgtact caattagaa aacgtttct 
gctaaatga ataaagaaa aacaatagt taagattga 
acaatgttt cgctttttc tagattgac tgaagaggt 
aactaaaaa agttaagaa acaaaaata attagtttt 
ctgttgata attgcagta tttttcgat ataagtatt 
tactaaaaa tacagggtg tttattttt gtttaaaaa 
tgttaaaaa taaaacttc tactaatag taaagtaat 
aaaaaaagc gaagccact attgtatct actccatta 
aataagtat ttttccaaa gagttttgc cattcactt 
ttgagcgct ttaaaacag tttgctata aaatctaaa 
aggaaaaaa acacatttt ttgccatac aaatgagtt 
tttctttat aattttcaa tttttacgg agaaatttg 
acaagagtg agattttgt agcatcata attagcaaa 
acttttcca tcaactcaa caaagaaaa aattaatta 
cgtgtggct ttttgcgac tggcttaag aaataatca 
aacaagtta aaaacctta acaagagga aatttgaaa 
agataaaga atgagctaa caaaactgc tggaaacgt 
aataaaaaa aacgcaaaa gtttaaaag ttcgaatag 
ttttctcta gataattgc agtacttcg tataaacgg 
tatatttaa aatacaggg ctcttttaa atgactttg 
gtgttcgtc gtatttttt attttttgg tgtttttct 
aaataatta acatggtgc tgaatcgag tgattcttt 
tctgtttta taattattc acttcaaga aaaataatt 
cttgaatga aagtacgct aataacgaa acaccaaat 
tttatactt ttttttagt cttcgatgt tttagatag 
attacataa attggctta tttgacggt taagtaacc 
ggttaaaca ccacaaaca caagcatgg ttttcagct 
aagtttgca aaaaaatca aaggcagac ataaacata 
aaaactcaa acatgaaaa ccaagctgt acttccttt 
ggggactgc gataaaatg aaaaacctg aagtaaaca 
tttccagta tcagtgtta aaaaactaa gattttatc 
gtagaataa tgaaatttt ctactgaag atgtatcac 
aatttgagc tcgcgcaaa ctattttca tgggaaatt 
tttttgttt tcaaaatag cacaatttt acactgccc 
tgttgaaaa gatcttgtt tagctacat gctgcctac 
gttttagtt gatatcaaa ataattaaa aaaaaacta 
atatcaaag taattaaag aaagggtat tttccatcc 
atggaacgt cttaacttt tgactgatt aaaagagag 
ttgggtata ttaattttt aattaacca actttaagt 
taattggtt ggaagtttc agtgcatat caaattgaa 
attggggac tgaaccgtt tatatcatt ttcgaagaa 
ttttcagaa acctctagt cgttgtaat aaacaatca 
aattgaaat tgcgtagcg taatgttgt aggtagaat 
aagaaattc acttgtctt gctctaact gtaaaggca 
cactagtgt atgaaactt tatcagctc ggatctatt 
ccatttctg ttcaccatt cctctcgtc aagtgcttg 
cacatttga gggcaaaca gctgagaat cttccaatg 
taaccaaat gtgtgagtg catttgtaa aaagttgca 
agtttgttc attccattc gctaggtta ccagtgctt 
tattttcta gttggtgtt tgtgagagc taatttttc 
aatgctgga aaattagct cgtgtcgca gttcgcata 
ggaaattgt ccttctagt tgataacta actaaaaat 
gcactctgt tttttgtgt ttttcgctc gattctctt 
acttcatgt tgaaacaac gtatcatca tggcataag 
taacaaatg tgatacatc atttagtgc atatttgtg 
aaagtgtta gttgattat ctattaatt aatattttt 
ctacttgca catattgta acataggca taacagaca 
tattactct agatgttta acgtaagtt tgtaacgca 
tgcaagggg ttgtggagc ctcttgtgt gcgacatat 
attatgtag atgggtatg ataacgaag ccaggagcc 
gctctctac gtaaaattg aattattaa cacttattg 
atggcagat attctttga atttttaac acaaaatat 
tatgacaat taaataata tacgtatta tttggcgaa 
tttgttgtt tttggtgtg tgaggagcg tgtgtgata 
cggtgtgat ttaagggag atgactcgg ttaactcgg 
tacagggag cggcagtga gctgttcgc aagaccatg 
gggaagcag aatagtaaa ctgaaaccg gaagttttg 
gaagatctt aaacagaac acagaattc tcagacgct 
gagattcaa gaatggtac aaaggattc ctcaaggac 
tgccctagc ggccacctt tccgtcgac gaatttaaa 
aagatctac ggtaacttt tttccttac ggtgatgcg 
agcaagttt gcagaacat gtttttcgt acatttgat 
gcgaacgga gatggcacc atcgatttt cgggagttt 
ctgtgcgcg ctgtccgtc acctctcga ggaaaacta 
gaacaaaag ctcaaatgg gccttttca atgtacgac 
ctagacggt aatggttat atatcacgg caagagatg 
ctcgaaata gtcacggca atctacaag atggtgggg 
tctgtgatg aagatgcct gaagacgag agtacgccc 
gagaaacgt accgacaaa atcttccgc cagatggac 
cgaaacaag gacggcaaa ctaagcctc gaggagttc 
atcgaggga gcaaaaagc gatccttcc atcgtacga 
ctcctccaa tgcgacccc caagcgcag tagcctaag 
accgcaaag ccggtcacc agagactct ttatcttaa 
gtttttatc aaccactct tgtgtgatc gcggggttt 
ccgcgtcga catcccggt tatataata actagagtt 
agaaaaaga tggatgtct gtctggatt cgtaagtaa 
aacatcacg ttgttgtgt ttccattta accgcggat 
tgttatgct caccctcgc atagatttt agtgacaga 
atatttaat cctaattta atttagaaa taactttaa 
gcagaacta tctacctac ttatattga atttcagtg 
gcgatctat aacttataa tattgcaat tgatgatat 
ctcaagtac tctactatc actatccaa atatatcaa 
ttgaatgtt actgttttc aaagttttt atgtgattc 
gagaaacgt tgaatgtag atggtatat tttattgta 
atattctaa tgttatctc gatggtaat tggtgctta 
actgtcggg cggactcgc gaccgaaat tagtttaga 
atttcatgc cgaaacaag ctcattgct gcgattttt 
ctaaattta aaatgtatc tcaaaaact aatattcaa 
atcagtgtt tgctgttaa atactggtt gagttaatt 
ttttcggaa tatttctac aattcctta taattttat 
aattaaaat tgattaatt ttaaaaaat tattattta 
tgtcattct aaaacaagg tgcttaaat gttgtctgt 
tccaaaaat atgataaac aatgccgtt agtcacaaa 
aacaaaata aataaaaag agttttaat ctaaacata 
tctgaaaat ttgcatttg tgttgtttt tttctattt 
attcagtct ttaattgaa ggttcattg gtgattcaa 
taatcacaa aaataagta aaaaacctt gtctaaaat 
ggagatttt aaacgaaat tttctcttt ttgttacat 
gggatactt ttttttggt tatttcttc gtttttttt 
tttttttaa atactttgt caaagtgtc aaaaatctc 
ggaattaat tcgaaaatg atattactt tgtttgtta 
cacttgaat ttagttggt ttttgatgg caatttccc 
caaaaaaaa attggagac acaaaattt ggtagaaac 
ttctaacgt ctaacttac cttctcagt tttttgtga 
aattttgtt aaattatac cgaaaaatc gcaaaattg 
gatcaaaaa tgatttctt agagtcaat cgaaatttt 
gtaaaaaaa taacactat tatagcttt tttcttgcg 
tttattagc acgtttgca agttagtaa catattttt 
tttgcaaaa tttcgtcac aaacaggcc aaaaatgta 
tcaaatcaa gccaaaaat gatgttaaa tttgccgtt 
ctacagtgt ttaggcatg tttttgaaa taaaaacta 
aagtttttt ctgggat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY