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Gene information
General information
TC010474 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451027.1: 236419..271038
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010474
    iBeetle screen iB_04845

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010474 RA
      MGCFGSKDK LSKEDMDFL KSHTRYDES 
      TIKEWYKGF KQDCPNGRL TPAKFVDMY 
      KMFFPSGNA EEFCDHVFR TFDMDKNGY 
      IDFKEFLLA IDVTSSGTP EEKLKWAFR 
      MYDVDGNGV IDIQEMTKI VQAIYDMLG 
      ACSSNRPAD SAEERAKNI FAKMDENND 
      GQLTQDEFL KGCLQDEEL SKMLAP
    CDS sequence
    • >TC010474 RA
      atgggatgc ttcggcagc aaagacaag ctgtccaag 
      gaagatatg gattttttg aaaagccac acccgttat 
      gacgaaagc acaataaaa gaatggtac aaaggcttt 
      aagcaagac tgcccaaat ggacgtttg accccagct 
      aaattcgta gacatgtac aagatgttc ttcccgtct 
      ggaaatgcc gaagagttt tgtgatcac gtttttcga 
      actttcgat atggacaaa aacggttac atcgacttt 
      aaggaattc ctgttggcg atcgatgta acgtcgagc 
      ggcactcca gaagaaaaa ctcaaatgg gctttccgt 
      atgtacgac gtggatggg aatggtgtt atagacatc 
      caggaaatg actaaaatc gttcaggct atttacgac 
      atgttgggc gcctgttcg tccaaccgc cctgcggat 
      tccgccgaa gagcgggca aagaacatc ttcgcgaaa 
      atggacgag aacaacgac ggccaactc acccaggac 
      gagtttctc aaaggatgc ttgcaggac gaagagctg 
      tcgaaaatg ctagctcct taa
    mRNA sequence
    • >TC010474 RA
      gcgcgttag tttagcgtg ttcgtgttt ccaccgttc 
      tgtctttct tcggcgcct ccaaattcc cataatgcc 
      aatttaaaa gtgaagtgt tgtacattt tattcgctt 
      ttttcgcgc ggatgatgg accacaaca cactcatcc 
      attctagga cgaattatc cttaatttt ttcacgctt 
      gaatcgccg ctcataaac gatgaaaaa atactagtg 
      acgtacacc acctgcaac acaataacg cgcacacca 
      agggattaa ccagaattt cagcgcaga ctacggtga 
      aaggctgcg cgaggcaaa atgggatgc ttcggcagc 
      aaagacaag ctgtccaag gaagatatg gattttttg 
      aaaagccac acccgttat gacgaaagc acaataaaa 
      gaatggtac aaaggcttt aagcaagac tgcccaaat 
      ggacgtttg accccagct aaattcgta gacatgtac 
      aagatgttc ttcccgtct ggaaatgcc gaagagttt 
      tgtgatcac gtttttcga actttcgat atggacaaa 
      aacggttac atcgacttt aaggaattc ctgttggcg 
      atcgatgta acgtcgagc ggcactcca gaagaaaaa 
      ctcaaatgg gctttccgt atgtacgac gtggatggg 
      aatggtgtt atagacatc caggaaatg actaaaatc 
      gttcaggct atttacgac atgttgggc gcctgttcg 
      tccaaccgc cctgcggat tccgccgaa gagcgggca 
      aagaacatc ttcgcgaaa atggacgag aacaacgac 
      ggccaactc acccaggac gagtttctc aaaggatgc 
      ttgcaggac gaagagctg tcgaaaatg ctagctcct 
      taatgtgca gcagcagcg atgcgattg agcgcggaa 
      atgtgtctt aagctgatg gaggcgttg aagagactt 
      tgtgcgagc aactcttgt cagaccacg ggtacattt 
      tcatatact ttcgaatcg acgaaacca tccgagatt 
      tgtagttgt gcatgagtt aacaacatc gatcacagc 
      tttgtattt attgcccca ttgatttag tccaaccat 
      ggtacgttt tattacgga ttaagaggt gaaagaatt 
      aaacgagaa ctaactaac acgacaatt aaccacgac 
      caataactc tacagggtg tttgattga tgagtgaca 
      atccatatc gatatattt cttacgcaa ttttgagct 
      ggaagcaac accctgtct aaagaccaa gtcttaaat 
      gataaaagc taaagtcaa ttatttgta aaaggcgta 
      gaagaggta ggtaggtag tgtgtagcg cgatcgaag 
      tttgtcgtc aaccgcgta ctagttcgt gtttctcat 
      gagaataat ttcattgat ttcgttcga aactgccat 
      atatttttg tacgtacgt acgtagact gtaaaccag 
      tttgcgtga ttttaatcc gtaataaga gcatgccgc 
      tttcaagca tttcgaacc aattttttt acaaaccgt 
      taattcata actatgtac ttagataat tcaactaga 
      gcttagaca aatcgcgtg cacaatccg cacattatt 
      atatcagtt caggtcgat ttataaatg ggtgtttac 
      aatatggat gtatttacc aatttacca ttttaagca 
      tatcatgag tgttactta ctgtcctgt gtagtgacc 
      catttgact attttttcc gaattgaaa tcgttggtg 
      tgagaatga gcgcgaacg ttgaacttg tcgttttct 
      aggtgttaa ttgattggc atcctccta agcgaattt 
      ttatgtaat ctatctact gtttgaaaa aaatatttg 
      cctgttgat taggcggta aattactga gtcaatttt 
      cttgtagca gcagtgggg tcaaaatat tttttccta 
      atataacat taaataaaa ataataaat caagtgaaa 
      attaagtcc aaggtgagt ctactaaaa gtatttttc 
      gttgaataa tgtgtatgt ttttctgat taaaattaa 
      ttaattgat attaagtca ctgaaaaat ttaattgta 
      atcaaattc tcatttgtc tttgatttg gtcgttgcc 
      aaataaata cagtaaaac ttctcaaca acggacagc 
      gatggggaa catgtccgc tgtggagag gtgtccgct 
      aatgggagg tgttacgtt cctacaaaa atgtccgtt 
      gtaaagagg tgtccgtta agggaggtt ttactgtag 
      taatttaag tcaaattac ttttattaa tttttttaa 
      gttaacgat tttgtacga aatgtttgc acaatacaa 
      cataaattc atcgattta ttaataaaa aaaatcagt 
      atcttaatt ttagataaa ttatttgtt gtagataaa 
      ttttgaaaa aatctcata catacggaa aaaatgtat 
      actgaatag caaacacct acatatttc tgtgtgatt 
      attattagt gtttgaaat acagtgaaa tatacttta 
      gtctcgctt ttagtaaac tcacactgt acaccaggc 
      caccaagaa tgtttaaat tggcaataa aacttctgc 
      taactgaag taagaaaaa aaaaatagt gcactacgt 
      gcgaacgtt ttgccattt aataaaaaa aaatctggt 
      aacaagata atcggaaat aggcaaaag ttattaaaa 
      gttataata gtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY