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Gene information
General information
TC010464 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7719190..7722634
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010464
    iBeetle screen iB_04842

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: L1 larva cuticle visible under L2 larva cuticle
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010464 RA
      MDAAVASTL PDNVLEVIF SYLSLHDLR NCSLVCKRW 
      YSFLNDENN DVWRLHCIR KLAEEALKS DLLSSVPTY 
      KAKLRAFYH AWNPNDCSR NIYIKPNGF TLHRNPVAQ 
      STDASRGKI GFRHGRHAW EVIWEGPLG TVAVIGIAT 
      KDAPLQCHG YVALLGSDD QSWGWNLVD NHLLHNGDA 
      QGNYPLLNN APKYQVGER IRVILDCDD NTLSFEKNY 
      EFLGVAFRG LPDKKLYPT VSAVYGNTE VSMVYLGPP 
      LDG
    CDS sequence
    • >TC010464 RA
      atggatgcc gctgtagca tcaacattg cccgataat 
      gtgttggaa gtaattttt tcatatttg tctctgcat 
      gatttgagg aactgttct ttagtgtgt aagagatgg 
      tatagtttt ttaaatgat gaaaataac gacgtttgg 
      aggctgcac tgtattcgg aaactagcc gaagaagca 
      ctaaagtca gatttgttg tcttcagtt cctacgtat 
      aaagcgaaa ttgcgggcg ttttaccac gcctggaat 
      ccgaacgac tgctctaga aatatttat attaagcct 
      aatgggttc acgttacac cgaaatccc gtagctcaa 
      agcacggac gcttcgaga ggtaaaatc ggatttaga 
      catgggaga cacgcttgg gaggtgatc tgggaaggg 
      cccttgggg acggtcgcc gtcatagga attgccaca 
      aaagacgca cctctgcag tgtcacggt tacgtggcc 
      ctgctgggc tcggacgac cagagttgg ggctggaac 
      ttagttgat aatcattta ctgcacaat ggagatgct 
      caaggcaat tacccgcta cttaacaat gcccctaaa 
      tatcaagtt ggggagcga attcgggtg attttagat 
      tgtgatgac aacacgcta tcttttgaa aaaaactac 
      gagtttttg ggagtggcc tttagagga ttgccagat 
      aaaaaattg tatccaaca gtttctgcc gtttacgga 
      aacactgaa gtttcaatg gtgtatctc ggaccacct 
      ttagatggc taa
    mRNA sequence
    • >TC010464 RA
      ccgttcaaa aatggatgc cgctgtagc atcaacatt 
      gcccgataa tgtgttgga agtaatttt ttcatattt 
      gtctctgca tgatttgag gaactgttc tttagtgtg 
      taagagatg gtatagttt tttaaatga tgaaaataa 
      cgacgtttg gaggctgca ctgtattcg gaaactagc 
      cgaagaagc actaaagtc agatttgtt gtcttcagt 
      tcctacgta taaagcgaa attgcgggc gttttacca 
      cgcctggaa tccgaacga ctgctctag aaatattta 
      tattaagcc taatgggtt cacgttaca ccgaaatcc 
      cgtagctca aagcacgga cgcttcgag aggtaaaat 
      cggatttag acatgggag acacgcttg ggaggtgat 
      ctgggaagg gcccttggg gacggtcgc cgtcatagg 
      aattgccac aaaagacgc acctctgca gtgtcacgg 
      ttacgtggc cctgctggg ctcggacga ccagagttg 
      gggctggaa cttagttga taatcattt actgcacaa 
      tggagatgc tcaaggcaa ttacccgct acttaacaa 
      tgcccctaa atatcaagt tggggagcg aattcgggt 
      gattttaga ttgtgatga caacacgct atcttttga 
      aaaaaacta cgagttttt gggagtggc ctttagagg 
      attgccaga taaaaaatt gtatccaac agtttctgc 
      cgtttacgg aaacactga agtttcaat ggtgtatct 
      cggaccacc tttagatgg ctaacacag ctggaagtt 
      gacgttagt ctgttaatt aactgaaaa aaggggttg 
      gatgcttca agatggctc attcaggac agcaggttt 
      gcccatgtg ataattaca ttttacttc agtcagtca 
      tacttccat taaaaatga gtatgtagg acaatttaa 
      acaatggta gatgtagaa tttttataa cagtagact 
      aagagaggt gcatttgaa gtatatgtt gtcacaaac 
      ttaactaaa taacagtcc acaaggagg aattgattt 
      cgttttaat acgttttat ttatttttt agatgctac 
      cacttttta catcaaatg cactaagta aataaacat 
      tttgccata ttccgtgac ctgattgcc actttttta 
      acactgaaa cagaaacat tgagtgagt agactttta 
      gggaatcaa ttcaaattg ggtgctttc ttgcaattt 
      tttgttgca atattttca tcattactg ctataaaat 
      tgcctccca gtaacaaat tttaataac actgccaaa 
      atacaggag ttacgcagc tgaaataaa agacaatga 
      aaaaagtcg taattatta aaaattgta atgacgtgg 
      tttgatatc tatctgttt aatgagtat cgaaatttg 
      catatgaat gcataatgg tgtttttag tgatcgttt 
      gaccccaga tatgttttt atgcaaagt agaacaaac 
      atttttatt gaaaaaacg gaggtaatt ttatagtag 
      caatgacct ccaggaccg gatcgagac tttatttat 
      ttttaggaa caaattcat cgaaagttc aggatattt 
      ttagacaag ggttgacca aaaaattac aaaataaaa 
      aaataaatt gttttatcg aaaaaaaaa aaattgagc 
      cttttacca atagtttaa cacattttg ttacaaatg 
      aacggaaat atcacgcga gtgatttaa atgtggagc 
      aatttttgc aattttcga actttttta agtacgttt 
      gacatgaaa ttgttacac aaaaattcc tgattgggt 
      taaaaatgc attattttt gcgtatttt agcgtttaa 
      gtgatactt ttttggttc aaaaacagt gatattgtt 
      gcttattta aacacagtc ttctgaaga aatattatt 
      tatcagtaa gttactaat tgttttaac tttttaccc 
      tcgatccgg ccttggtgg tgccaattt tgtagaatg 
      actacattt ttgattaat tttttacgt tggcagtca 
      atgtagtcg tgtacaagt ctcgaaaaa taagccatt 
      gttgccttg taatttcag aactttgtt ttcttgttt 
      gtttctgtg atattttcg agcaaatgt ttatttatc 
      atgatttta aaattcttg atctggtat taagcttgt 
      tgtttttta tattattta ttatttatt ttgccacga 
      ccagtagtt acgtaactt tgtaaccat gaaatgtaa 
      gcatattcg tcctcactc tgcttcgca aagtaataa 
      attgataaa tgtaacttt ttgtcattg caaatgcat 
      ttttgctat ttgaaccca aaatttcct aatgcaaac 
      gcaagcctg aatatttca attattttg ggtttcgat 
      agcatcgga tatattttt ttatcgatg aaagtgatg 
      taactcatc ttgtacagg tccaaaggt ttggataag 
      tttgcagat atctaacaa atgcattaa tgaaaccat 
      aaattgtac taaagcgta taatcttga tttgttacc 
      aatgtttta ggttgtact cttgtaaat atgtatgta 
      tgtacgttt acatttttt caagtaatc gatacttaa 
      atagtatta aaataaatt ctagtcatt tgtattaac 
      gagctgtta atgcgtatc tcggcattt tgttcggct 
      tggatgtat gagcactat tctaaaatt caaattgtc 
      caccttgaa gttgacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY