Status
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Gene information
General information
TC033101
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010460
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7560700..7599493
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033101
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04841

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033101 RA
      MAGLLLLGL LACAPVAST EIPLLILSE LCYYPLNMD 
      YIDQELGNF SLKILPPED VCEDPSKLL HILSTSRAP 
      LLGSLSSEF CSPAYIIAN FYNTTLVTW NCPKTTRGI 
      DTTRDLRLA PSIPTITRG FAKVVANMK WKSVAIVAS 
      NTSPLQPAD AVDHCRTEH ASVWGVYSN MLTRKVAIS 
      GDGWWPSLS QSIDIQLRE FGIIPRHFF MIGRNSSRR 
      HLENKLRIL KRISCRAVI TCLAADEAG KTVLEVIDN 
      LQLAHDHNT LTVFMDVSN LLSWKYALA QKNATEAGN 
      GTNFLDAVS SVSKKCILI YSEADNASL ESAVLRNVH 
      KSIARLGGL QEQQEAFLL SGLHEDGAF RPLLAVSRD 
      NASEFVVAE VADNLEEWM LKHKQLDEC EGCESELAK 
      TLSVLGILG CSCLFVTVL LGTVALARS QLLKKRVSK 
      GPYKVLLTA TDFVFPQIA DSRRVDEGI EAMLCCWLQ 
      QLQEFGGPE VDKPDLLQG SGASVKTPL RGGSSPNLA 
      KQLIVDPRV RYNGDLVQM KPVPSAGGT VELKAKTVE 
      LLVLLHGLR HENLNPLIG CLAEPPRAA LVSEYCARG 
      SLQDVLQQD DIKLDWSFR LSLLTDLVR GMKYLHSTP 
      IRVHGYLTS RNCVIDARW VLKVTDYGL PAFYEAQGM 
      QPPAKTARE LLWTAPELL RHASLRKKG TQPGDVYSF 
      GIVLQEVVV RGEPFCMLA LTPEEIIEK VKRPPPLIR 
      PSVSKGAAP PEAINIMRQ CWAEQAEMR PDFNTVHDQ 
      FKKLNHGRK VNIVDTMFQ MLEKYSNNL EELIRERTE 
      QLDIEKKKT EQLLNRMLP SSVAEKLKL GMPVDPEEF 
      EEVTIYFSD IVGFTTISA HSTPFQVVD LLNDLYTCF 
      DATINAYNV YKVETIGDA YMVVGGLPV RVPDHAEQI 
      ATMALDLLH QSGRFRITH LPGTPLRLR IGLHTGPCC 
      AGVVGLTMP RYCLFGDTV NTASRMEST GAAWRIHLS 
      EATKERLEK AGGYQLEYR GPTEIKGKG TMHTYWLLG 
      KAGFDKPLP VPPSLELDE SLIISKSIS GNLKHEETT 
      TSGDSISVE SRQQPFSED HQPKSPNPN PSFIKTMET 
      SKFSSLVKT QDETATMRH DYHMVKSTS SDTPMSLGT 
      PVSATEVAA ALLGASTSS LCGFRRHRK IEEEDLSTP 
      YNHYKCLSP KHRIGKLLR RQFSLDRTE EIRTEESLQ 
      TSPRLFKQN SAGAADLEK IEEVPMKLP VSPAPSTTP 
      LQCTLSISA DSLIH
    • >TC033101 RB
      MAGLLLLGL LACAPVAST EIPLLILSE LCYYPLNMD 
      YIDQELGNF SLKILPPED VCEDPSKLL HILSTSRAP 
      LLGSLSSEF CSPAYIIAN FYNTTLVTW NCPKTTRGI 
      DTTRDLRLA PSIPTITRG FAKVVANMK WKSVAIVAS 
      NTSPLQPAD AVDHCRTEH ASVWGVYSN MLTRKVAIS 
      GDGWWPSLS QSIDIQLRE FGIIPRHFF MIGRNSSRR 
      HLENKLRIL KRISCRAVI TCLAADEAG KTVLEVIDN 
      LQLAHDHNT LTVFMDVSN LLSWKYALA QKNATEAGN 
      GTNFLDAVS SVSKKCILI YSEADNASL ESAVLRNVH 
      KSIARLGGL QEQQEAFLL SGLHEDGAF RPLLAVSRD 
      NASEFVVAE VADNLEEWM LKHKQLDEC EGCESELAK 
      TLSVLGILG CSCLFVTVL LGTVALARS QLLKKRVSK 
      GPYKVLLTA TDFVFPQIA DSRRVDEGI EAMLCCWLQ 
      QLQEFGGPE VDKPDLLQG SGASVKTPL RGGSSPNLA 
      KQLIVDPRV RYNGDLVQM KPVPSAGGT VELKAKTVE 
      LLVLLHGLR HENLNPLIG CLAEPPRAA LVSEYCARG 
      SLQDVLQQD DIKLDWSFR LSLLTDLVR GMKYLHSTP 
      IRVHGYLTS RNCVIDARW VLKVTDYGL PAFYEAQGM 
      QPPAKTARE LLWTAPELL RHASLRKKG TQPGDVYSF 
      GIVLQEVVV RGEPFCMLA LTPEEIIEK VKRPPPLIR 
      PSVSKGAAP PEAINIMRQ CWAEQAEMR PDFNTVHDQ 
      FKKLNHGRK VNIVDTMFQ MLEKYSNNL EELIRERTE 
      QLDIEKKKT EQLLNRMLP SSVAEKLKL GMPVDPEEF 
      EEVTIYFSD IVGFTTISA HSTPFQVVD LLNDLYTCF 
      DATINAYNV YKVETIGDA YMVVGGLPV RVPDHAEQI 
      ATMALDLLH QSGRFRITH LPGTPLRLR IGLHTGPCC 
      AGVVGLTMP RYCLFGDTV NTASRMEST GAAWRIHLS 
      EATKERLEK AGGYQLEYR GPTEIKGKG TMHTYWLLG 
      KAGFDKPLP VPPSLELDE SLIISKSIS GNLKHEETT 
      TSGDSISVE SRQQPFSED HQPKSPNPN PSFIKTMET 
      SKFSSLVKT QDETATMRH DYHMVKSTS SDTPMSLGT 
      PVSATEVAA ALLGASTSS LCGFRRHRK IEEEDLSTP 
      YNHYKCLSP KHRIGKLLR RQFSLDRTE EIRTEESLQ 
      FLFQTSPRL FKQNSAGAA DLEKIEEVP MKLPVSPAP 
      STTPLQCTL SISADSLIH
    CDS sequence
    • >TC033101 RA
      atggcaggc ctgctgcta ttggggttg ctggcctgc 
      gcccccgtg gcttctaca gaaatccct cttcttatc 
      ctttccgaa ctctgctat tatcccctc aacatggat 
      tacatcgat caagaatta ggaaatttc agcctaaaa 
      atcctgcct ccggaagat gtttgtgag gacccttcc 
      aagctgctg cacatatta tcaacaagt agggctcct 
      cttttgggc tcgctcagt tcggagttc tgctctccg 
      gcttacatt atcgcaaat ttttataat accactctc 
      gtaacctgg aactgtcca aagactaca agaggaatc 
      gatacaact cgagattta agactcgct ccctcgatt 
      cctacaata actagggga tttgcgaag gttgtggcc 
      aacatgaag tggaaatca gtcgcaatt gttgccagc 
      aatacatct ccattacag ccagcagat gcggtagat 
      cactgtcga actgagcac gcaagtgtc tggggggtt 
      tacagcaac atgttgact cggaaagtt gcaatttcg 
      ggagacggg tggtggcct tcgctgtca caatccatc 
      gacatacag ctacgagag tttggaatt attccgcga 
      cactttttc atgataggg aggaacagc tcgaggaga 
      caccttgag aataagttg agaattttg aagaggatt 
      tcgtgccga gctgtaata acgtgtcta gctgcggat 
      gaggccggc aagacggtg ttggaggta attgataac 
      ctgcaactg gcccacgat cacaacaca ttaacggtg 
      ttcatggac gtatcgaat ttgctctcg tggaagtac 
      gctctcgcg caaaaaaac gccaccgag gcgggcaac 
      ggcacaaat ttcctggac gcggtcagt tcggtcagc 
      aaaaagtgc attctaatc tacagcgag gccgacaac 
      gcgagtttg gagagtgcc gttttgagg aacgttcac 
      aagtccatc gcgcgtttg ggcggcctg caggagcag 
      caggaggcg ttcctgctg tcgggcctg cacgaggac 
      ggcgcgttc cggccgctt ctagccgtc agcagggac 
      aatgcgagt gagtttgtg gtggcggag gttgcggac 
      aacctggag gagtggatg ctgaagcac aaacagttg 
      gacgagtgc gaggggtgt gaaagcgaa ctcgccaaa 
      acgctgagt gtgttgggg atcctgggg tgctcctgc 
      ctgtttgtc acggtgctc cttgggacg gtggcgctg 
      gccaggagt cagctgctg aagaagcgc gtctccaag 
      gggccctac aaggtgttg ttgaccgcc accgatttc 
      gtcttcccc cagatcgcc gacagtcgc agggtagat 
      gaaggtatc gaggcgatg ttgtgctgt tggcttcag 
      cagctgcag gaatttgga ggtcctgaa gtggataag 
      ccggatcta ttacaaggc tcaggggca tcggtgaaa 
      acgccatta cgtggaggg tccagtccg aatctggca 
      aaacaactc atcgtggac cctcgagtc cgatataat 
      ggcgatttg gtgcaaatg aagccggtg ccttcggca 
      ggcggcacc gtcgagttg aaggcgaaa accgtggaa 
      ctgttggtg ttacttcac ggcttaagg cacgagaac 
      ctaaatccg ctgatagga tgtctggca gagccgcca 
      agagcagcg ctcgtgagc gaatactgc gctcgggga 
      tctttgcag gacgtgctc cagcaggat gacatcaag 
      ctcgactgg tctttccgc ttgagtctg cttacggat 
      ttagttcgg ggcatgaaa taccttcat tccacaccg 
      atccgagtg cacggctat ttgacatct cgtaattgc 
      gttatcgat gcgagatgg gtgctgaaa gtcacagat 
      tacggatta cccgccttc tacgaagca caagggatg 
      caaccccca gccaagact gccagagag ctcttgtgg 
      acggctccc gagctgctg cgacacgct agtttaagg 
      aaaaaagga acacagccc ggagatgtg tattcattt 
      gggattgtt ttacaagaa gtggtcgtt agaggggaa 
      ccattctgt atgctcgct ttgacaccc gaagaaatt 
      atcgaaaaa gtgaaacgg cctccgcct ctgatccgt 
      ccctcggtc agtaaagga gcagctcct ccggaagcc 
      atcaatata atgcgtcag tgttgggcc gaacaggcc 
      gaaatgcgc ccggacttc aacacagtc cacgaccaa 
      ttcaagaaa ttaaatcac ggcagaaaa gtcaacatc 
      gtggatacg atgttccaa atgctcgaa aagtactcc 
      aacaacttg gaggagttg atacgcgaa cggaccgag 
      cagctcgac attgagaag aaaaagacc gagcaactt 
      ttaaaccga atgcttccg agttccgtg gctgaaaag 
      ctgaagttg ggcatgccc gtggacccg gaggagttc 
      gaggaagtg acgatttat ttctcggac attgtcggt 
      ttcacgaca atatcagcc cattcgacc ccctttcag 
      gtggtcgat ctcttgaac gatctttat acatgtttc 
      gacgccacg atcaacgct tacaacgtc tacaaagtt 
      gaaaccatc ggagatgct tatatggtt gttggagga 
      ttgccggtt cgtgttccc gatcacgcc gaacaaatt 
      gcgacaatg gccctggat ttgctgcac caaagcggc 
      cgttttcgc atcacccac ctcccgggc acgccgctg 
      cggctgagg atcggcctg cacacaggc ccctgctgt 
      gccggcgtt gtcggctta acgatgcca agatactgt 
      ctatttggt gatactgtt aacacagct tcaagaatg 
      gaatcaaca ggtgccgct tggaggata cacttatcc 
      gaggcaacg aaggagcgg ctggagaag gcgggcggc 
      tatcagctc gaatataga ggtcccacg gaaataaaa 
      ggaaaaggg acaatgcac acgtattgg ttgctggga 
      aaagcgggc ttcgataaa ccgcttccg gtgcctccg 
      tctttggaa ttagacgaa agtcttatt atctctaag 
      tcgatatct ggtaatctg aagcacgag gaaacgaca 
      acgtcgggt gattcgatc tcggtggaa tcgcgtcaa 
      cagcctttc agcgaagat catcagcca aaatcaccc 
      aacccgaat ccttcgttt attaaaaca atggaaact 
      tccaaattc tcaagtctc gtcaaaacg caggacgaa 
      acggcgacc atgagacac gattatcac atggtaaaa 
      agcacatca agtgacact ccaatgtct ctaggaacc 
      ccggtgtcg gccaccgaa gttgcggct gctttactg 
      ggggcttca accagttca ctttgtgga tttaggcga 
      caccgcaag attgaagag gaagatttg agtactcca 
      tataatcat tacaaatgc ctcagtccg aagcaccga 
      attggaaaa ctactaagg aggcaattt agtttagat 
      agaacggag gaaatacga acggaggaa tccctacaa 
      acgagtccc agactgttc aagcagaat tcggcagga 
      gcggcagat ttggaaaaa attgaagaa gttccaatg 
      aaacttcca gtttcgcct gcaccaagt acaaccccc 
      ctgcagtgc actttgtct atatcagca gattctctt 
      attcattga
    • >TC033101 RB
      atggcaggc ctgctgcta ttggggttg ctggcctgc 
      gcccccgtg gcttctaca gaaatccct cttcttatc 
      ctttccgaa ctctgctat tatcccctc aacatggat 
      tacatcgat caagaatta ggaaatttc agcctaaaa 
      atcctgcct ccggaagat gtttgtgag gacccttcc 
      aagctgctg cacatatta tcaacaagt agggctcct 
      cttttgggc tcgctcagt tcggagttc tgctctccg 
      gcttacatt atcgcaaat ttttataat accactctc 
      gtaacctgg aactgtcca aagactaca agaggaatc 
      gatacaact cgagattta agactcgct ccctcgatt 
      cctacaata actagggga tttgcgaag gttgtggcc 
      aacatgaag tggaaatca gtcgcaatt gttgccagc 
      aatacatct ccattacag ccagcagat gcggtagat 
      cactgtcga actgagcac gcaagtgtc tggggggtt 
      tacagcaac atgttgact cggaaagtt gcaatttcg 
      ggagacggg tggtggcct tcgctgtca caatccatc 
      gacatacag ctacgagag tttggaatt attccgcga 
      cactttttc atgataggg aggaacagc tcgaggaga 
      caccttgag aataagttg agaattttg aagaggatt 
      tcgtgccga gctgtaata acgtgtcta gctgcggat 
      gaggccggc aagacggtg ttggaggta attgataac 
      ctgcaactg gcccacgat cacaacaca ttaacggtg 
      ttcatggac gtatcgaat ttgctctcg tggaagtac 
      gctctcgcg caaaaaaac gccaccgag gcgggcaac 
      ggcacaaat ttcctggac gcggtcagt tcggtcagc 
      aaaaagtgc attctaatc tacagcgag gccgacaac 
      gcgagtttg gagagtgcc gttttgagg aacgttcac 
      aagtccatc gcgcgtttg ggcggcctg caggagcag 
      caggaggcg ttcctgctg tcgggcctg cacgaggac 
      ggcgcgttc cggccgctt ctagccgtc agcagggac 
      aatgcgagt gagtttgtg gtggcggag gttgcggac 
      aacctggag gagtggatg ctgaagcac aaacagttg 
      gacgagtgc gaggggtgt gaaagcgaa ctcgccaaa 
      acgctgagt gtgttgggg atcctgggg tgctcctgc 
      ctgtttgtc acggtgctc cttgggacg gtggcgctg 
      gccaggagt cagctgctg aagaagcgc gtctccaag 
      gggccctac aaggtgttg ttgaccgcc accgatttc 
      gtcttcccc cagatcgcc gacagtcgc agggtagat 
      gaaggtatc gaggcgatg ttgtgctgt tggcttcag 
      cagctgcag gaatttgga ggtcctgaa gtggataag 
      ccggatcta ttacaaggc tcaggggca tcggtgaaa 
      acgccatta cgtggaggg tccagtccg aatctggca 
      aaacaactc atcgtggac cctcgagtc cgatataat 
      ggcgatttg gtgcaaatg aagccggtg ccttcggca 
      ggcggcacc gtcgagttg aaggcgaaa accgtggaa 
      ctgttggtg ttacttcac ggcttaagg cacgagaac 
      ctaaatccg ctgatagga tgtctggca gagccgcca 
      agagcagcg ctcgtgagc gaatactgc gctcgggga 
      tctttgcag gacgtgctc cagcaggat gacatcaag 
      ctcgactgg tctttccgc ttgagtctg cttacggat 
      ttagttcgg ggcatgaaa taccttcat tccacaccg 
      atccgagtg cacggctat ttgacatct cgtaattgc 
      gttatcgat gcgagatgg gtgctgaaa gtcacagat 
      tacggatta cccgccttc tacgaagca caagggatg 
      caaccccca gccaagact gccagagag ctcttgtgg 
      acggctccc gagctgctg cgacacgct agtttaagg 
      aaaaaagga acacagccc ggagatgtg tattcattt 
      gggattgtt ttacaagaa gtggtcgtt agaggggaa 
      ccattctgt atgctcgct ttgacaccc gaagaaatt 
      atcgaaaaa gtgaaacgg cctccgcct ctgatccgt 
      ccctcggtc agtaaagga gcagctcct ccggaagcc 
      atcaatata atgcgtcag tgttgggcc gaacaggcc 
      gaaatgcgc ccggacttc aacacagtc cacgaccaa 
      ttcaagaaa ttaaatcac ggcagaaaa gtcaacatc 
      gtggatacg atgttccaa atgctcgaa aagtactcc 
      aacaacttg gaggagttg atacgcgaa cggaccgag 
      cagctcgac attgagaag aaaaagacc gagcaactt 
      ttaaaccga atgcttccg agttccgtg gctgaaaag 
      ctgaagttg ggcatgccc gtggacccg gaggagttc 
      gaggaagtg acgatttat ttctcggac attgtcggt 
      ttcacgaca atatcagcc cattcgacc ccctttcag 
      gtggtcgat ctcttgaac gatctttat acatgtttc 
      gacgccacg atcaacgct tacaacgtc tacaaagtt 
      gaaaccatc ggagatgct tatatggtt gttggagga 
      ttgccggtt cgtgttccc gatcacgcc gaacaaatt 
      gcgacaatg gccctggat ttgctgcac caaagcggc 
      cgttttcgc atcacccac ctcccgggc acgccgctg 
      cggctgagg atcggcctg cacacaggc ccctgctgt 
      gccggcgtt gtcggctta acgatgcca agatactgt 
      ctatttggt gatactgtt aacacagct tcaagaatg 
      gaatcaaca ggtgccgct tggaggata cacttatcc 
      gaggcaacg aaggagcgg ctggagaag gcgggcggc 
      tatcagctc gaatataga ggtcccacg gaaataaaa 
      ggaaaaggg acaatgcac acgtattgg ttgctggga 
      aaagcgggc ttcgataaa ccgcttccg gtgcctccg 
      tctttggaa ttagacgaa agtcttatt atctctaag 
      tcgatatct ggtaatctg aagcacgag gaaacgaca 
      acgtcgggt gattcgatc tcggtggaa tcgcgtcaa 
      cagcctttc agcgaagat catcagcca aaatcaccc 
      aacccgaat ccttcgttt attaaaaca atggaaact 
      tccaaattc tcaagtctc gtcaaaacg caggacgaa 
      acggcgacc atgagacac gattatcac atggtaaaa 
      agcacatca agtgacact ccaatgtct ctaggaacc 
      ccggtgtcg gccaccgaa gttgcggct gctttactg 
      ggggcttca accagttca ctttgtgga tttaggcga 
      caccgcaag attgaagag gaagatttg agtactcca 
      tataatcat tacaaatgc ctcagtccg aagcaccga 
      attggaaaa ctactaagg aggcaattt agtttagat 
      agaacggag gaaatacga acggaggaa tccctacaa 
      ttcttgttt cagacgagt cccagactg ttcaagcag 
      aattcggca ggagcggca gatttggaa aaaattgaa 
      gaagttcca atgaaactt ccagtttcg cctgcacca 
      agtacaacc cccctgcag tgcactttg tctatatca 
      gcagattct cttattcat tga
    mRNA sequence
    • >TC033101 RA
      actctctca cgagggaga cgccgacgc ggatcgtcg 
      tccggagaa caactagcg atggaattc actgtcaac 
      ttttgcaat taccacgag gcagcctaa gcaaaaatg 
      catgaggca caccgtgcc atgcctcgt tgaggaacg 
      tgtcatggc aggcctgct gctattggg gttgctggc 
      ctgcgcccc cgtggcttc tacagaaat ccctcttct 
      tatcctttc cgaactctg ctattatcc cctcaacat 
      ggattacat cgatcaaga attaggaaa tttcagcct 
      aaaaatcct gcctccgga agatgtttg tgaggaccc 
      ttccaagct gctgcacat attatcaac aagtagggc 
      tcctctttt gggctcgct cagttcgga gttctgctc 
      tccggctta cattatcgc aaattttta taataccac 
      tctcgtaac ctggaactg tccaaagac tacaagagg 
      aatcgatac aactcgaga tttaagact cgctccctc 
      gattcctac aataactag gggatttgc gaaggttgt 
      ggccaacat gaagtggaa atcagtcgc aattgttgc 
      cagcaatac atctccatt acagccagc agatgcggt 
      agatcactg tcgaactga gcacgcaag tgtctgggg 
      ggtttacag caacatgtt gactcggaa agttgcaat 
      ttcgggaga cgggtggtg gccttcgct gtcacaatc 
      catcgacat acagctacg agagtttgg aattattcc 
      gcgacactt tttcatgat agggaggaa cagctcgag 
      gagacacct tgagaataa gttgagaat tttgaagag 
      gatttcgtg ccgagctgt aataacgtg tctagctgc 
      ggatgaggc cggcaagac ggtgttgga ggtaattga 
      taacctgca actggccca cgatcacaa cacattaac 
      ggtgttcat ggacgtatc gaatttgct ctcgtggaa 
      gtacgctct cgcgcaaaa aaacgccac cgaggcggg 
      caacggcac aaatttcct ggacgcggt cagttcggt 
      cagcaaaaa gtgcattct aatctacag cgaggccga 
      caacgcgag tttggagag tgccgtttt gaggaacgt 
      tcacaagtc catcgcgcg tttgggcgg cctgcagga 
      gcagcagga ggcgttcct gctgtcggg cctgcacga 
      ggacggcgc gttccggcc gcttctagc cgtcagcag 
      ggacaatgc gagtgagtt tgtggtggc ggaggttgc 
      ggacaacct ggaggagtg gatgctgaa gcacaaaca 
      gttggacga gtgcgaggg gtgtgaaag cgaactcgc 
      caaaacgct gagtgtgtt ggggatcct ggggtgctc 
      ctgcctgtt tgtcacggt gctccttgg gacggtggc 
      gctggccag gagtcagct gctgaagaa gcgcgtctc 
      caaggggcc ctacaaggt gttgttgac cgccaccga 
      tttcgtctt cccccagat cgccgacag tcgcagggt 
      agatgaagg tatcgaggc gatgttgtg ctgttggct 
      tcagcagct gcaggaatt tggaggtcc tgaagtgga 
      taagccgga tctattaca aggctcagg ggcatcggt 
      gaaaacgcc attacgtgg agggtccag tccgaatct 
      ggcaaaaca actcatcgt ggaccctcg agtccgata 
      taatggcga tttggtgca aatgaagcc ggtgccttc 
      ggcaggcgg caccgtcga gttgaaggc gaaaaccgt 
      ggaactgtt ggtgttact tcacggctt aaggcacga 
      gaacctaaa tccgctgat aggatgtct ggcagagcc 
      gccaagagc agcgctcgt gagcgaata ctgcgctcg 
      gggatcttt gcaggacgt gctccagca ggatgacat 
      caagctcga ctggtcttt ccgcttgag tctgcttac 
      ggatttagt tcggggcat gaaatacct tcattccac 
      accgatccg agtgcacgg ctatttgac atctcgtaa 
      ttgcgttat cgatgcgag atgggtgct gaaagtcac 
      agattacgg attacccgc cttctacga agcacaagg 
      gatgcaacc cccagccaa gactgccag agagctctt 
      gtggacggc tcccgagct gctgcgaca cgctagttt 
      aaggaaaaa aggaacaca gcccggaga tgtgtattc 
      atttgggat tgttttaca agaagtggt cgttagagg 
      ggaaccatt ctgtatgct cgctttgac acccgaaga 
      aattatcga aaaagtgaa acggcctcc gcctctgat 
      ccgtccctc ggtcagtaa aggagcagc tcctccgga 
      agccatcaa tataatgcg tcagtgttg ggccgaaca 
      ggccgaaat gcgcccgga cttcaacac agtccacga 
      ccaattcaa gaaattaaa tcacggcag aaaagtcaa 
      catcgtgga tacgatgtt ccaaatgct cgaaaagta 
      ctccaacaa cttggagga gttgatacg cgaacggac 
      cgagcagct cgacattga gaagaaaaa gaccgagca 
      acttttaaa ccgaatgct tccgagttc cgtggctga 
      aaagctgaa gttgggcat gcccgtgga cccggagga 
      gttcgagga agtgacgat ttatttctc ggacattgt 
      cggtttcac gacaatatc agcccattc gaccccctt 
      tcaggtggt cgatctctt gaacgatct ttatacatg 
      tttcgacgc cacgatcaa cgcttacaa cgtctacaa 
      agttgaaac catcggaga tgcttatat ggttgttgg 
      aggattgcc ggttcgtgt tcccgatca cgccgaaca 
      aattgcgac aatggccct ggatttgct gcaccaaag 
      cggccgttt tcgcatcac ccacctccc gggcacgcc 
      gctgcggct gaggatcgg cctgcacac aggcccctg 
      ctgtgccgg cgttgtcgg cttaacgat gccaagata 
      ctgtctatt tggtgatac tgttaacac agcttcaag 
      aatggaatc aacaggtgc cgcttggag gatacactt 
      atccgaggc aacgaagga gcggctgga gaaggcggg 
      cggctatca gctcgaata tagaggtcc cacggaaat 
      aaaaggaaa agggacaat gcacacgta ttggttgct 
      gggaaaagc gggcttcga taaaccgct tccggtgcc 
      tccgtcttt ggaattaga cgaaagtct tattatctc 
      taagtcgat atctggtaa tctgaagca cgaggaaac 
      gacaacgtc gggtgattc gatctcggt ggaatcgcg 
      tcaacagcc tttcagcga agatcatca gccaaaatc 
      acccaaccc gaatccttc gtttattaa aacaatgga 
      aacttccaa attctcaag tctcgtcaa aacgcagga 
      cgaaacggc gaccatgag acacgatta tcacatggt 
      aaaaagcac atcaagtga cactccaat gtctctagg 
      aaccccggt gtcggccac cgaagttgc ggctgcttt 
      actgggggc ttcaaccag ttcactttg tggatttag 
      gcgacaccg caagattga agaggaaga tttgagtac 
      tccatataa tcattacaa atgcctcag tccgaagca 
      ccgaattgg aaaactact aaggaggca atttagttt 
      agatagaac ggaggaaat acgaacgga ggaatccct 
      acaaacgag tcccagact gttcaagca gaattcggc 
      aggagcggc agatttgga aaaaattga agaagttcc 
      aatgaaact tccagtttc gcctgcacc aagtacaac 
      ccccctgca gtgcacttt gtctatatc agcagattc 
      tcttattca ttgagacaa ggtcggcga aacgggaaa 
      atcgacgtg aaacaataa taattatta gacaatcac 
      cttcgtctg atttatttt gtacttttt caaatggat 
      taattgtgt ctcccaaaa agtaaatca aaaatgcgc 
      tgttaaacc ggtaattta caatttctg atgatgttc 
      catctgtaa attaacatt tttaagtgt tacattgta 
      aattcagcg ttctttatt tctcgtgca actgctgtc 
      taaatctaa aattctaat ttgtataaa gtgtagact 
      ttgaaagga aaagaaacg cacactctt gaataatct 
      aatagttac attatacat accctttac aacgccatc 
      aacaatttg tctccgaat gactaatga gtgtgttac 
      ttaagtgga ctctaggac atttgtctt actttcttc 
      tagtttgaa tgtgagttt gtttatagt tgcaattgt 
      cgaacgttt gaatgtaac taaaattgg gtagacaat 
      cctctcaag tctctaaat agactcaaa atcaatgtt 
      gatatgttt aaatatata taaatgttg ttaaaacgt 
      attattatt agatttatc aacattttt tctattcga 
      atttattca catgatttt atctgtaaa taaacacaa 
      gagaaattt
    • >TC033101 RB
      actctctca cgagggaga cgccgacgc ggatcgtcg 
      tccggagaa caactagcg atggaattc actgtcaac 
      ttttgcaat taccacgag gcagcctaa gcaaaaatg 
      catgaggca caccgtgcc atgcctcgt tgaggaacg 
      tgtcatggc aggcctgct gctattggg gttgctggc 
      ctgcgcccc cgtggcttc tacagaaat ccctcttct 
      tatcctttc cgaactctg ctattatcc cctcaacat 
      ggattacat cgatcaaga attaggaaa tttcagcct 
      aaaaatcct gcctccgga agatgtttg tgaggaccc 
      ttccaagct gctgcacat attatcaac aagtagggc 
      tcctctttt gggctcgct cagttcgga gttctgctc 
      tccggctta cattatcgc aaattttta taataccac 
      tctcgtaac ctggaactg tccaaagac tacaagagg 
      aatcgatac aactcgaga tttaagact cgctccctc 
      gattcctac aataactag gggatttgc gaaggttgt 
      ggccaacat gaagtggaa atcagtcgc aattgttgc 
      cagcaatac atctccatt acagccagc agatgcggt 
      agatcactg tcgaactga gcacgcaag tgtctgggg 
      ggtttacag caacatgtt gactcggaa agttgcaat 
      ttcgggaga cgggtggtg gccttcgct gtcacaatc 
      catcgacat acagctacg agagtttgg aattattcc 
      gcgacactt tttcatgat agggaggaa cagctcgag 
      gagacacct tgagaataa gttgagaat tttgaagag 
      gatttcgtg ccgagctgt aataacgtg tctagctgc 
      ggatgaggc cggcaagac ggtgttgga ggtaattga 
      taacctgca actggccca cgatcacaa cacattaac 
      ggtgttcat ggacgtatc gaatttgct ctcgtggaa 
      gtacgctct cgcgcaaaa aaacgccac cgaggcggg 
      caacggcac aaatttcct ggacgcggt cagttcggt 
      cagcaaaaa gtgcattct aatctacag cgaggccga 
      caacgcgag tttggagag tgccgtttt gaggaacgt 
      tcacaagtc catcgcgcg tttgggcgg cctgcagga 
      gcagcagga ggcgttcct gctgtcggg cctgcacga 
      ggacggcgc gttccggcc gcttctagc cgtcagcag 
      ggacaatgc gagtgagtt tgtggtggc ggaggttgc 
      ggacaacct ggaggagtg gatgctgaa gcacaaaca 
      gttggacga gtgcgaggg gtgtgaaag cgaactcgc 
      caaaacgct gagtgtgtt ggggatcct ggggtgctc 
      ctgcctgtt tgtcacggt gctccttgg gacggtggc 
      gctggccag gagtcagct gctgaagaa gcgcgtctc 
      caaggggcc ctacaaggt gttgttgac cgccaccga 
      tttcgtctt cccccagat cgccgacag tcgcagggt 
      agatgaagg tatcgaggc gatgttgtg ctgttggct 
      tcagcagct gcaggaatt tggaggtcc tgaagtgga 
      taagccgga tctattaca aggctcagg ggcatcggt 
      gaaaacgcc attacgtgg agggtccag tccgaatct 
      ggcaaaaca actcatcgt ggaccctcg agtccgata 
      taatggcga tttggtgca aatgaagcc ggtgccttc 
      ggcaggcgg caccgtcga gttgaaggc gaaaaccgt 
      ggaactgtt ggtgttact tcacggctt aaggcacga 
      gaacctaaa tccgctgat aggatgtct ggcagagcc 
      gccaagagc agcgctcgt gagcgaata ctgcgctcg 
      gggatcttt gcaggacgt gctccagca ggatgacat 
      caagctcga ctggtcttt ccgcttgag tctgcttac 
      ggatttagt tcggggcat gaaatacct tcattccac 
      accgatccg agtgcacgg ctatttgac atctcgtaa 
      ttgcgttat cgatgcgag atgggtgct gaaagtcac 
      agattacgg attacccgc cttctacga agcacaagg 
      gatgcaacc cccagccaa gactgccag agagctctt 
      gtggacggc tcccgagct gctgcgaca cgctagttt 
      aaggaaaaa aggaacaca gcccggaga tgtgtattc 
      atttgggat tgttttaca agaagtggt cgttagagg 
      ggaaccatt ctgtatgct cgctttgac acccgaaga 
      aattatcga aaaagtgaa acggcctcc gcctctgat 
      ccgtccctc ggtcagtaa aggagcagc tcctccgga 
      agccatcaa tataatgcg tcagtgttg ggccgaaca 
      ggccgaaat gcgcccgga cttcaacac agtccacga 
      ccaattcaa gaaattaaa tcacggcag aaaagtcaa 
      catcgtgga tacgatgtt ccaaatgct cgaaaagta 
      ctccaacaa cttggagga gttgatacg cgaacggac 
      cgagcagct cgacattga gaagaaaaa gaccgagca 
      acttttaaa ccgaatgct tccgagttc cgtggctga 
      aaagctgaa gttgggcat gcccgtgga cccggagga 
      gttcgagga agtgacgat ttatttctc ggacattgt 
      cggtttcac gacaatatc agcccattc gaccccctt 
      tcaggtggt cgatctctt gaacgatct ttatacatg 
      tttcgacgc cacgatcaa cgcttacaa cgtctacaa 
      agttgaaac catcggaga tgcttatat ggttgttgg 
      aggattgcc ggttcgtgt tcccgatca cgccgaaca 
      aattgcgac aatggccct ggatttgct gcaccaaag 
      cggccgttt tcgcatcac ccacctccc gggcacgcc 
      gctgcggct gaggatcgg cctgcacac aggcccctg 
      ctgtgccgg cgttgtcgg cttaacgat gccaagata 
      ctgtctatt tggtgatac tgttaacac agcttcaag 
      aatggaatc aacaggtgc cgcttggag gatacactt 
      atccgaggc aacgaagga gcggctgga gaaggcggg 
      cggctatca gctcgaata tagaggtcc cacggaaat 
      aaaaggaaa agggacaat gcacacgta ttggttgct 
      gggaaaagc gggcttcga taaaccgct tccggtgcc 
      tccgtcttt ggaattaga cgaaagtct tattatctc 
      taagtcgat atctggtaa tctgaagca cgaggaaac 
      gacaacgtc gggtgattc gatctcggt ggaatcgcg 
      tcaacagcc tttcagcga agatcatca gccaaaatc 
      acccaaccc gaatccttc gtttattaa aacaatgga 
      aacttccaa attctcaag tctcgtcaa aacgcagga 
      cgaaacggc gaccatgag acacgatta tcacatggt 
      aaaaagcac atcaagtga cactccaat gtctctagg 
      aaccccggt gtcggccac cgaagttgc ggctgcttt 
      actgggggc ttcaaccag ttcactttg tggatttag 
      gcgacaccg caagattga agaggaaga tttgagtac 
      tccatataa tcattacaa atgcctcag tccgaagca 
      ccgaattgg aaaactact aaggaggca atttagttt 
      agatagaac ggaggaaat acgaacgga ggaatccct 
      acaattctt gtttcagac gagtcccag actgttcaa 
      gcagaattc ggcaggagc ggcagattt ggaaaaaat 
      tgaagaagt tccaatgaa acttccagt ttcgcctgc 
      accaagtac aacccccct gcagtgcac tttgtctat 
      atcagcaga ttctcttat tcattgaga caaggtcgg 
      cgaaacggg aaaatcgac gtgaaacaa taataatta 
      ttagacaat caccttcgt ctgatttat tttgtactt 
      tttcaaatg gattaattg tgtctccca aaaagtaaa 
      tcaaaaatg cgctgttaa accggtaat ttacaattt 
      ctgatgatg ttccatctg taaattaac atttttaag 
      tgttacatt gtaaattca gcgttcttt atttctcgt 
      gcaactgct gtctaaatc taaaattct aatttgtat 
      aaagtgtag actttgaaa ggaaaagaa acgcacact 
      cttgaataa tctaatagt tacattata cataccctt 
      tacaacgcc atcaacaat ttgtctccg aatgactaa 
      tgagtgtgt tacttaagt ggactctag gacatttgt 
      cttactttc ttctagttt gaatgtgag tttgtttat 
      agttgcaat tgtcgaacg tttgaatgt aactaaaat 
      tgggtagac aatcctctc aagtctcta aatagactc 
      aaaatcaat gttgatatg tttaaatat atataaatg 
      ttgttaaaa cgtattatt attagattt atcaacatt 
      ttttctatt cgaatttat tcacatgat tttatctgt 
      aaataaaca caagagaaa ttt
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