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Gene information
General information
TC010449 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7462150..7463922
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010449
    iBeetle screen iB_04839

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands darker - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection


    prothoracic odoriferous glands darker - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    Adult morphology
    prothoracic odoriferous glands colour darker - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    abdominal odoriferous glands spotted - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    abdominal odoriferous glands colour lighter - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection

    Comment: abdominal glands appear lighter with one dark spot inside

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010449 RA
      MTTPSREEN GPPGENVKV TVVGDGVVG 
KTCLLISYT KDEFPEEYV PTVFEHYGQ 
DITVDDVKY NMTLWDTAG QEDYERLRP 
LAYPNTKCF LVCFSVDAS IPSYENVII 
KWVPEVRHH NPHTPIVLV ATKIDLRDD 
PSVHCYSTQ DGKKLKRKV KAEGYVECS 
AKTREGLKE VFEEAIRAY KKTKIKARQ 
VNCALL
    CDS sequence
    • >TC010449 RA
      atgacgacg ccttcgaga gaagaaaac ggccctccg 
ggcgaaaac gttaaagtc acagtggtc ggagacggt 
gtagtggga aaaacctgt ctcttgatt tcatacacg 
aaggacgag ttccccgaa gaatatgtt cccacagta 
ttcgaacat tacgggcaa gacatcacc gtcgacgac 
gtcaaatac aacatgact ctctgggac actgccggc 
caagaagac tacgaaaga ctgcgcccc ctggcttac 
cccaatacg aaatgcttc cttgtatgt ttctccgtc 
gacgcttct ataccgtct tacgagaac gttataatt 
aaatgggtg ccagaagtc aggcaccac aatccacac 
acgcccata gttctagta gccactaaa atagatctc 
agagatgat ccaagtgtc cactgttac tcaacccag 
gacggcaaa aaattaaaa aggaaagtc aaagccgaa 
ggatacgtc gaatgctct gccaaaacc agggaagga 
ctgaaggaa gtcttcgaa gaggcgata cgagcgtac 
aagaagacc aaaatcaaa gcgagacaa gtcaactgt 
gctttgtta taa
    mRNA sequence
    • >TC010449 RA
      aacgtgtta acatttttc agtgccagt gacgttttt 
cctgtcctt gctgaatta aataaattc aaatgatga 
aaaacagag acgtaacgt gtgaagaat gtatcgata 
aacaatagt ttcgcctta ttgatgtag atatttcgt 
ctgaataat aataattaa cgccacata ccgagctaa 
ctcgccttt gcttattta attcagcaa acgtagtgt 
aggaatgag cgaaatgaa tcatgatgt gatagtgca 
gtgtgaaaa tgactccaa ttaagaaat tatcaaaat 
aactgaaac gagctttat taacggaaa caggtcaca 
taaatttat gaaaaactc gctcatctt gctaccagc 
aatccgtca cggagaaag gttgataaa ctaacaata 
aatttacaa gtgaagcgc ggctcttga taatccgag 
ataacaatt tcctaagcg ataatgaac gccacgatt 
tgtgctatt atttagtgg aacttaata tttccaaac 
caattttgg aaaatgagt gattaacgt gactgggga 
aattttcca aatttagtc atgacgacg ccttcgaga 
gaagaaaac ggccctccg ggcgaaaac gttaaagtc 
acagtggtc ggagacggt gtagtggga aaaacctgt 
ctcttgatt tcatacacg aaggacgag ttccccgaa 
gaatatgtt cccacagta ttcgaacat tacgggcaa 
gacatcacc gtcgacgac gtcaaatac aacatgact 
ctctgggac actgccggc caagaagac tacgaaaga 
ctgcgcccc ctggcttac cccaatacg aaatgcttc 
cttgtatgt ttctccgtc gacgcttct ataccgtct 
tacgagaac gttataatt aaatgggtg ccagaagtc 
aggcaccac aatccacac acgcccata gttctagta 
gccactaaa atagatctc agagatgat ccaagtgtc 
cactgttac tcaacccag gacggcaaa aaattaaaa 
aggaaagtc aaagccgaa ggatacgtc gaatgctct 
gccaaaacc agggaagga ctgaaggaa gtcttcgaa 
gaggcgata cgagcgtac aagaagacc aaaatcaaa 
gcgagacaa gtcaactgt gctttgtta taacatgcg 
agtaatgat attattcaa aatacgtat tgtattttc 
acatttatg gttcagtca gcaatatat tggacgatt 
tcattagtt taaacgaaa tctcggctc atgtgcatt 
attacatta tatcgattt ttatcactg aataaacag 
caattttgt tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY