General information | |||
TC010440 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7327580..7329350 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC010440 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04836 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdominal segment 2 tracheal opening not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment potentiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
- >TC010440 RA
MADENNTPP ANPEEPSQE AETGEANVK KISITVKSQ KQKEVIEVE ETADVKSFK ELISEKFNA EIDRLNLIF AGKILKDGD TLSQHNIRD GLTVYLVVK AAPAPDSTS TRPPGDGAQ SQTSFNPFG GLVGLSSMG MNSTNLVEI QQRLQREIQ NNPAILQSY LDNPLTQSL MNNPEHMRT LITSNPQMQ ELLERNPEI GHMLNNPEL LRQTMELAR NPSLYQELL RSNDRAMAN LESIPGGFN ALQQMYRDI QEPMLTSLS EQFTQNPFA GLSESNNST TTQPDPNTR SIPNPWTSQ PNSGSQNRS VASVMQQML ENSDLMQNM LSAPYMQDL LAALSANPS MANTLLSEN PLLAGNPAL QEQIRNMMP QFLQQLQNP SMQNVLSNP QALNAIMQI QQGMETLRQ TAPNLLNPA NATSETVTS TTTSPSEST PRAAPAGAP PSDNFSEFM ARMVAGMAN QRENLPPEQ RYQAQLEQL TSMGFTNRE ANLQALIAT FGDVSAAIE RLLLSDT
- >TC010440 RA
atggcagat gaaaataat acgcctccg gccaatcct gaggaacct agtcaagaa gctgaaacg ggggaagca aacgtcaag aaaatcagc atcactgtt aagtcgcaa aagcagaag gaagtaatc gaagttgag gaaacggca gatgttaaa tcgttcaag gagttgatt tccgagaaa tttaatgcg gagattgac cgcctgaat ctgattttc gccgggaaa atcctcaaa gatggggac actttatcc cagcataac ataagggat ggattgacc gtgtacctg gtggtcaaa gcggccccc gcgccggac tccacctcc acgcggccc ccaggtgac ggagcgcag tcccaaact agttttaat ccatttggg gggttagta ggtttaagt tcaatgggg atgaactcc accaacttg gtggaaatc cagcagcgt ttgcagaga gaaatccaa aataacccg gcgatactt cagtcgtac ctagacaac cccctcact caaagtttg atgaacaac cctgagcac atgaggacg ctaatcacc agcaacccc cagatgcag gagctacta gaacgcaac ccggaaatc ggacacatg ctcaacaac cctgagctc ctgagacag acaatggag ttggcgcgc aacccctcc ctgtaccag gaactactg cgaagcaac gaccgcgca atggccaac ctggagagc atccccggg gggttcaac gcccttcaa caaatgtac agagacatc caagaaccg atgttaaca agcctcagc gagcagttc acccagaac cccttcgcg gggctttcc gagagcaac aacagtact accacccaa cccgacccc aacacccgg tccatcccc aacccctgg acaagccaa ccaaactct ggctctcag aaccggtca gtggccagt gtcatgcag caaatgctg gaaaactca gatctgatg caaaacatg ctctcagcc ccctacatg caggacctt ctggcagcc ctgagcgcc aacccctcc atggccaac accctcctc tctgagaac cccctccta gctgggaac ccagccttg caggagcag attcgcaac atgatgccc cagtttttg caacagttg cagaacccc agcatgcag aatgtgctc tcgaatccg caggccctc aacgccatc atgcaaatc cagcaaggg atggaaacg ctgcgacag acagccccg aatttgctc aacccggcc aatgccacg tcggagact gtcaccagc actacgacc agccccagc gagagcacc cccagggct gcgcccgcg ggggcgccc ccaagcgac aatttctcc gaatttatg gctcgcatg gtggccggg atggccaac cagagggag aatttgccc ccagaacag cggtatcaa gcgcagttg gagcagctg acctccatg gggttcacc aatcgcgag gcgaatttg caagcgctg attgccacc tttggggac gtttcagcg gcgattgag agattgttg ctttcagac acgtga
- >TC010440 RA
gtgtcaaaa taagaaaac tgaaacaac tcattcgag aattttgta aatcttacg aaaccagcg aattctcgt agtgattat catggcaga tgaaaataa tacgcctcc ggccaatcc tgaggaacc tagtcaaga agctgaaac gggggaagc aaacgtcaa gaaaatcag catcactgt taagtcgca aaagcagaa ggaagtaat cgaagttga ggaaacggc agatgttaa atcgttcaa ggagttgat ttccgagaa atttaatgc ggagattga ccgcctgaa tctgatttt cgccgggaa aatcctcaa agatgggga cactttatc ccagcataa cataaggga tggattgac cgtgtacct ggtggtcaa agcggcccc cgcgccgga ctccacctc cacgcggcc cccaggtga cggagcgca gtcccaaac tagttttaa tccatttgg ggggttagt aggtttaag ttcaatggg gatgaactc caccaactt ggtggaaat ccagcagcg tttgcagag agaaatcca aaataaccc ggcgatact tcagtcgta cctagacaa ccccctcac tcaaagttt gatgaacaa ccctgagca catgaggac gctaatcac cagcaaccc ccagatgca ggagctact agaacgcaa cccggaaat cggacacat gctcaacaa ccctgagct cctgagaca gacaatgga gttggcgcg caacccctc cctgtacca ggaactact gcgaagcaa cgaccgcgc aatggccaa cctggagag catccccgg ggggttcaa cgcccttca acaaatgta cagagacat ccaagaacc gatgttaac aagcctcag cgagcagtt cacccagaa ccccttcgc ggggctttc cgagagcaa caacagtac taccaccca acccgaccc caacacccg gtccatccc caacccctg gacaagcca accaaactc tggctctca gaaccggtc agtggccag tgtcatgca gcaaatgct ggaaaactc agatctgat gcaaaacat gctctcagc cccctacat gcaggacct tctggcagc cctgagcgc caacccctc catggccaa caccctcct ctctgagaa ccccctcct agctgggaa cccagcctt gcaggagca gattcgcaa catgatgcc ccagttttt gcaacagtt gcagaaccc cagcatgca gaatgtgct ctcgaatcc gcaggccct caacgccat catgcaaat ccagcaagg gatggaaac gctgcgaca gacagcccc gaatttgct caacccggc caatgccac gtcggagac tgtcaccag cactacgac cagccccag cgagagcac ccccagggc tgcgcccgc gggggcgcc cccaagcga caatttctc cgaatttat ggctcgcat ggtggccgg gatggccaa ccagaggga gaatttgcc cccagaaca gcggtatca agcgcagtt ggagcagct gacctccat ggggttcac caatcgcga ggcgaattt gcaagcgct gattgccac ctttgggga cgtttcagc ggcgattga gagattgtt gctttcaga cacgtgaaa ggattaatt cgcattggg atggatgat ttagtctaa ttttgtgtt acttgaatg tcttcatta tatttaata tgaactttt taataaatg cacccttag gcc
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
