Status
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Gene information
General information
TC010440 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7327580..7329350
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010440
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04836

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdominal segment 2 tracheal opening not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment potentiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010440 RA
      MADENNTPP ANPEEPSQE AETGEANVK KISITVKSQ 
KQKEVIEVE ETADVKSFK ELISEKFNA EIDRLNLIF 
AGKILKDGD TLSQHNIRD GLTVYLVVK AAPAPDSTS 
TRPPGDGAQ SQTSFNPFG GLVGLSSMG MNSTNLVEI 
QQRLQREIQ NNPAILQSY LDNPLTQSL MNNPEHMRT 
LITSNPQMQ ELLERNPEI GHMLNNPEL LRQTMELAR 
NPSLYQELL RSNDRAMAN LESIPGGFN ALQQMYRDI 
QEPMLTSLS EQFTQNPFA GLSESNNST TTQPDPNTR 
SIPNPWTSQ PNSGSQNRS VASVMQQML ENSDLMQNM 
LSAPYMQDL LAALSANPS MANTLLSEN PLLAGNPAL 
QEQIRNMMP QFLQQLQNP SMQNVLSNP QALNAIMQI 
QQGMETLRQ TAPNLLNPA NATSETVTS TTTSPSEST 
PRAAPAGAP PSDNFSEFM ARMVAGMAN QRENLPPEQ 
RYQAQLEQL TSMGFTNRE ANLQALIAT FGDVSAAIE 
RLLLSDT
    CDS sequence
    • >TC010440 RA
      atggcagat gaaaataat acgcctccg gccaatcct 
gaggaacct agtcaagaa gctgaaacg ggggaagca 
aacgtcaag aaaatcagc atcactgtt aagtcgcaa 
aagcagaag gaagtaatc gaagttgag gaaacggca 
gatgttaaa tcgttcaag gagttgatt tccgagaaa 
tttaatgcg gagattgac cgcctgaat ctgattttc 
gccgggaaa atcctcaaa gatggggac actttatcc 
cagcataac ataagggat ggattgacc gtgtacctg 
gtggtcaaa gcggccccc gcgccggac tccacctcc 
acgcggccc ccaggtgac ggagcgcag tcccaaact 
agttttaat ccatttggg gggttagta ggtttaagt 
tcaatgggg atgaactcc accaacttg gtggaaatc 
cagcagcgt ttgcagaga gaaatccaa aataacccg 
gcgatactt cagtcgtac ctagacaac cccctcact 
caaagtttg atgaacaac cctgagcac atgaggacg 
ctaatcacc agcaacccc cagatgcag gagctacta 
gaacgcaac ccggaaatc ggacacatg ctcaacaac 
cctgagctc ctgagacag acaatggag ttggcgcgc 
aacccctcc ctgtaccag gaactactg cgaagcaac 
gaccgcgca atggccaac ctggagagc atccccggg 
gggttcaac gcccttcaa caaatgtac agagacatc 
caagaaccg atgttaaca agcctcagc gagcagttc 
acccagaac cccttcgcg gggctttcc gagagcaac 
aacagtact accacccaa cccgacccc aacacccgg 
tccatcccc aacccctgg acaagccaa ccaaactct 
ggctctcag aaccggtca gtggccagt gtcatgcag 
caaatgctg gaaaactca gatctgatg caaaacatg 
ctctcagcc ccctacatg caggacctt ctggcagcc 
ctgagcgcc aacccctcc atggccaac accctcctc 
tctgagaac cccctccta gctgggaac ccagccttg 
caggagcag attcgcaac atgatgccc cagtttttg 
caacagttg cagaacccc agcatgcag aatgtgctc 
tcgaatccg caggccctc aacgccatc atgcaaatc 
cagcaaggg atggaaacg ctgcgacag acagccccg 
aatttgctc aacccggcc aatgccacg tcggagact 
gtcaccagc actacgacc agccccagc gagagcacc 
cccagggct gcgcccgcg ggggcgccc ccaagcgac 
aatttctcc gaatttatg gctcgcatg gtggccggg 
atggccaac cagagggag aatttgccc ccagaacag 
cggtatcaa gcgcagttg gagcagctg acctccatg 
gggttcacc aatcgcgag gcgaatttg caagcgctg 
attgccacc tttggggac gtttcagcg gcgattgag 
agattgttg ctttcagac acgtga
    mRNA sequence
    • >TC010440 RA
      gtgtcaaaa taagaaaac tgaaacaac tcattcgag 
aattttgta aatcttacg aaaccagcg aattctcgt 
agtgattat catggcaga tgaaaataa tacgcctcc 
ggccaatcc tgaggaacc tagtcaaga agctgaaac 
gggggaagc aaacgtcaa gaaaatcag catcactgt 
taagtcgca aaagcagaa ggaagtaat cgaagttga 
ggaaacggc agatgttaa atcgttcaa ggagttgat 
ttccgagaa atttaatgc ggagattga ccgcctgaa 
tctgatttt cgccgggaa aatcctcaa agatgggga 
cactttatc ccagcataa cataaggga tggattgac 
cgtgtacct ggtggtcaa agcggcccc cgcgccgga 
ctccacctc cacgcggcc cccaggtga cggagcgca 
gtcccaaac tagttttaa tccatttgg ggggttagt 
aggtttaag ttcaatggg gatgaactc caccaactt 
ggtggaaat ccagcagcg tttgcagag agaaatcca 
aaataaccc ggcgatact tcagtcgta cctagacaa 
ccccctcac tcaaagttt gatgaacaa ccctgagca 
catgaggac gctaatcac cagcaaccc ccagatgca 
ggagctact agaacgcaa cccggaaat cggacacat 
gctcaacaa ccctgagct cctgagaca gacaatgga 
gttggcgcg caacccctc cctgtacca ggaactact 
gcgaagcaa cgaccgcgc aatggccaa cctggagag 
catccccgg ggggttcaa cgcccttca acaaatgta 
cagagacat ccaagaacc gatgttaac aagcctcag 
cgagcagtt cacccagaa ccccttcgc ggggctttc 
cgagagcaa caacagtac taccaccca acccgaccc 
caacacccg gtccatccc caacccctg gacaagcca 
accaaactc tggctctca gaaccggtc agtggccag 
tgtcatgca gcaaatgct ggaaaactc agatctgat 
gcaaaacat gctctcagc cccctacat gcaggacct 
tctggcagc cctgagcgc caacccctc catggccaa 
caccctcct ctctgagaa ccccctcct agctgggaa 
cccagcctt gcaggagca gattcgcaa catgatgcc 
ccagttttt gcaacagtt gcagaaccc cagcatgca 
gaatgtgct ctcgaatcc gcaggccct caacgccat 
catgcaaat ccagcaagg gatggaaac gctgcgaca 
gacagcccc gaatttgct caacccggc caatgccac 
gtcggagac tgtcaccag cactacgac cagccccag 
cgagagcac ccccagggc tgcgcccgc gggggcgcc 
cccaagcga caatttctc cgaatttat ggctcgcat 
ggtggccgg gatggccaa ccagaggga gaatttgcc 
cccagaaca gcggtatca agcgcagtt ggagcagct 
gacctccat ggggttcac caatcgcga ggcgaattt 
gcaagcgct gattgccac ctttgggga cgtttcagc 
ggcgattga gagattgtt gctttcaga cacgtgaaa 
ggattaatt cgcattggg atggatgat ttagtctaa 
ttttgtgtt acttgaatg tcttcatta tatttaata 
tgaactttt taataaatg cacccttag gcc
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