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Gene information
General information
TC010440 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7327580..7329350
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010440
    iBeetle screen iB_04836

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdominal segment 2 tracheal opening not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment potentiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010440 RA
      MADENNTPP ANPEEPSQE AETGEANVK KISITVKSQ 
      KQKEVIEVE ETADVKSFK ELISEKFNA EIDRLNLIF 
      AGKILKDGD TLSQHNIRD GLTVYLVVK AAPAPDSTS 
      TRPPGDGAQ SQTSFNPFG GLVGLSSMG MNSTNLVEI 
      QQRLQREIQ NNPAILQSY LDNPLTQSL MNNPEHMRT 
      LITSNPQMQ ELLERNPEI GHMLNNPEL LRQTMELAR 
      NPSLYQELL RSNDRAMAN LESIPGGFN ALQQMYRDI 
      QEPMLTSLS EQFTQNPFA GLSESNNST TTQPDPNTR 
      SIPNPWTSQ PNSGSQNRS VASVMQQML ENSDLMQNM 
      LSAPYMQDL LAALSANPS MANTLLSEN PLLAGNPAL 
      QEQIRNMMP QFLQQLQNP SMQNVLSNP QALNAIMQI 
      QQGMETLRQ TAPNLLNPA NATSETVTS TTTSPSEST 
      PRAAPAGAP PSDNFSEFM ARMVAGMAN QRENLPPEQ 
      RYQAQLEQL TSMGFTNRE ANLQALIAT FGDVSAAIE 
      RLLLSDT
    CDS sequence
    • >TC010440 RA
      atggcagat gaaaataat acgcctccg gccaatcct 
      gaggaacct agtcaagaa gctgaaacg ggggaagca 
      aacgtcaag aaaatcagc atcactgtt aagtcgcaa 
      aagcagaag gaagtaatc gaagttgag gaaacggca 
      gatgttaaa tcgttcaag gagttgatt tccgagaaa 
      tttaatgcg gagattgac cgcctgaat ctgattttc 
      gccgggaaa atcctcaaa gatggggac actttatcc 
      cagcataac ataagggat ggattgacc gtgtacctg 
      gtggtcaaa gcggccccc gcgccggac tccacctcc 
      acgcggccc ccaggtgac ggagcgcag tcccaaact 
      agttttaat ccatttggg gggttagta ggtttaagt 
      tcaatgggg atgaactcc accaacttg gtggaaatc 
      cagcagcgt ttgcagaga gaaatccaa aataacccg 
      gcgatactt cagtcgtac ctagacaac cccctcact 
      caaagtttg atgaacaac cctgagcac atgaggacg 
      ctaatcacc agcaacccc cagatgcag gagctacta 
      gaacgcaac ccggaaatc ggacacatg ctcaacaac 
      cctgagctc ctgagacag acaatggag ttggcgcgc 
      aacccctcc ctgtaccag gaactactg cgaagcaac 
      gaccgcgca atggccaac ctggagagc atccccggg 
      gggttcaac gcccttcaa caaatgtac agagacatc 
      caagaaccg atgttaaca agcctcagc gagcagttc 
      acccagaac cccttcgcg gggctttcc gagagcaac 
      aacagtact accacccaa cccgacccc aacacccgg 
      tccatcccc aacccctgg acaagccaa ccaaactct 
      ggctctcag aaccggtca gtggccagt gtcatgcag 
      caaatgctg gaaaactca gatctgatg caaaacatg 
      ctctcagcc ccctacatg caggacctt ctggcagcc 
      ctgagcgcc aacccctcc atggccaac accctcctc 
      tctgagaac cccctccta gctgggaac ccagccttg 
      caggagcag attcgcaac atgatgccc cagtttttg 
      caacagttg cagaacccc agcatgcag aatgtgctc 
      tcgaatccg caggccctc aacgccatc atgcaaatc 
      cagcaaggg atggaaacg ctgcgacag acagccccg 
      aatttgctc aacccggcc aatgccacg tcggagact 
      gtcaccagc actacgacc agccccagc gagagcacc 
      cccagggct gcgcccgcg ggggcgccc ccaagcgac 
      aatttctcc gaatttatg gctcgcatg gtggccggg 
      atggccaac cagagggag aatttgccc ccagaacag 
      cggtatcaa gcgcagttg gagcagctg acctccatg 
      gggttcacc aatcgcgag gcgaatttg caagcgctg 
      attgccacc tttggggac gtttcagcg gcgattgag 
      agattgttg ctttcagac acgtga
    mRNA sequence
    • >TC010440 RA
      gtgtcaaaa taagaaaac tgaaacaac tcattcgag 
      aattttgta aatcttacg aaaccagcg aattctcgt 
      agtgattat catggcaga tgaaaataa tacgcctcc 
      ggccaatcc tgaggaacc tagtcaaga agctgaaac 
      gggggaagc aaacgtcaa gaaaatcag catcactgt 
      taagtcgca aaagcagaa ggaagtaat cgaagttga 
      ggaaacggc agatgttaa atcgttcaa ggagttgat 
      ttccgagaa atttaatgc ggagattga ccgcctgaa 
      tctgatttt cgccgggaa aatcctcaa agatgggga 
      cactttatc ccagcataa cataaggga tggattgac 
      cgtgtacct ggtggtcaa agcggcccc cgcgccgga 
      ctccacctc cacgcggcc cccaggtga cggagcgca 
      gtcccaaac tagttttaa tccatttgg ggggttagt 
      aggtttaag ttcaatggg gatgaactc caccaactt 
      ggtggaaat ccagcagcg tttgcagag agaaatcca 
      aaataaccc ggcgatact tcagtcgta cctagacaa 
      ccccctcac tcaaagttt gatgaacaa ccctgagca 
      catgaggac gctaatcac cagcaaccc ccagatgca 
      ggagctact agaacgcaa cccggaaat cggacacat 
      gctcaacaa ccctgagct cctgagaca gacaatgga 
      gttggcgcg caacccctc cctgtacca ggaactact 
      gcgaagcaa cgaccgcgc aatggccaa cctggagag 
      catccccgg ggggttcaa cgcccttca acaaatgta 
      cagagacat ccaagaacc gatgttaac aagcctcag 
      cgagcagtt cacccagaa ccccttcgc ggggctttc 
      cgagagcaa caacagtac taccaccca acccgaccc 
      caacacccg gtccatccc caacccctg gacaagcca 
      accaaactc tggctctca gaaccggtc agtggccag 
      tgtcatgca gcaaatgct ggaaaactc agatctgat 
      gcaaaacat gctctcagc cccctacat gcaggacct 
      tctggcagc cctgagcgc caacccctc catggccaa 
      caccctcct ctctgagaa ccccctcct agctgggaa 
      cccagcctt gcaggagca gattcgcaa catgatgcc 
      ccagttttt gcaacagtt gcagaaccc cagcatgca 
      gaatgtgct ctcgaatcc gcaggccct caacgccat 
      catgcaaat ccagcaagg gatggaaac gctgcgaca 
      gacagcccc gaatttgct caacccggc caatgccac 
      gtcggagac tgtcaccag cactacgac cagccccag 
      cgagagcac ccccagggc tgcgcccgc gggggcgcc 
      cccaagcga caatttctc cgaatttat ggctcgcat 
      ggtggccgg gatggccaa ccagaggga gaatttgcc 
      cccagaaca gcggtatca agcgcagtt ggagcagct 
      gacctccat ggggttcac caatcgcga ggcgaattt 
      gcaagcgct gattgccac ctttgggga cgtttcagc 
      ggcgattga gagattgtt gctttcaga cacgtgaaa 
      ggattaatt cgcattggg atggatgat ttagtctaa 
      ttttgtgtt acttgaatg tcttcatta tatttaata 
      tgaactttt taataaatg cacccttag gcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY