Status
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Gene information
General information
TC010421 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7407816..7409633
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010421
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04834

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
      Days post injection


      remarks: animals lost,
      Pupal morphology
      remarks: animals lost,
      Adult morphology
      remarks: animals lost,
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010421 RA
        MWSRCSVGF CLLLAAQAA VLKTRNIPV EIVYDRTNP 
        ATYGTRNLD AKIQLSSDE LLKLKLSDD IINEEKIHF 
        EEGDAGNEQ GVKNLEEKI QLSSEEISH QSQNAVEID 
        GNIKAAVAE NSAQSGNLL DLLKRAEEM AYAGINTIR 
        ENFDKVSDS IPGAKPTAE AWEKLNQTL HDYFRDQYK 
        NALTLKQDQ PTSGNENPN QNQNQNQFF QNLINGIQT 
        ISNNFMGNF GQNGQTQKP PGSEDEQQP QQQQQGPWQ 
        SFVSYFQGG MNQMVTNMN NYFNRQNGT SGQEDTGTG 
        GNGGSNFIN NFVSSIQQF FQGQGGQGG SPSQTINVN 
        NQSDPGVTT NAPGFFQGA INQFNQVVN NFRPSTSAR 
        PPVQGDEGS STTPGNFIQ SIGQAFQNV NIFRPQGGQ 
        QGGSGGSSN PIQSIGSQF GGQGGNGSN PFVSGAQAI 
        GSQFAGQGG NGSNPFVSG AQAIGSQFG QAIGGQGGQ 
        GGGSNPLVS GAQAIGNQF QQAIGQGGN KPVEGGAGE 
        ATSEKTRAE SLVNEGEKV EKMKEKVEK KVEKVEEKV 
        EKKPAEMMA MAE
      CDS sequence
      • >TC010421 RA
        atgtggtca agatgttcg gtgggtttt tgtttgctc 
        cttgctgcc caggcagcg gtactcaaa acgagaaat 
        attcccgtt gaaattgtc tacgataga acgaacccg 
        gccacgtac gggacgaga aatttagac gccaaaata 
        caattatca tcggacgaa ttgctgaaa ttgaagctc 
        agtgatgat atcatcaac gaagaaaaa attcatttc 
        gaagaaggg gatgctggg aatgaacaa ggggtgaaa 
        aacttggag gagaaaatt caactgtcg tctgaagaa 
        attagccat cagtcgcaa aatgcggtg gaaattgac 
        ggcaatatt aaagctgcc gttgcggaa aattcggca 
        caatccggg aatttgctt gatcttttg aaacgggct 
        gaagagatg gcttacgct ggcattaac acgattagg 
        gagaacttt gataaggtc tcagatagt ataccgggg 
        gccaaacca acggcagaa gcttgggaa aaattaaac 
        caaactttg catgactac ttcagggat cagtacaag 
        aacgccctc actttgaaa caggatcag ccaacaagt 
        ggtaacgaa aaccccaat cagaatcaa aatcagaac 
        caatttttc caaaactta atcaatggt atccaaacc 
        atttccaac aactttatg ggcaatttt ggccaaaat 
        gggcaaacg cagaaaccg cctgggtcc gaggatgaa 
        caacaacca cagcagcaa caacaaggc ccgtggcaa 
        agcttcgtc agctatttc caaggagga atgaaccaa 
        atggtaacc aacatgaac aactatttc aaccgacag 
        aatggtacc tccgggcaa gaagacacc ggaactggg 
        ggtaacggg ggttcaaac ttcatcaac aactttgtc 
        agttcaatc cagcaattt ttccaaggc caggggggc 
        caggggggc agcccctcc caaacaatc aacgtcaac 
        aaccaaagc gaccccgga gtgaccacc aacgccccc 
        ggatttttt cagggcgcc attaaccaa ttcaaccaa 
        gtggttaac aacttccgg ccttcgacc tccgcccgg 
        cccccggtg caaggtgac gaaggctcg tcaacaact 
        ccggggaat tttatccaa agcataggc caggcgttc 
        cagaacgtt aacattttc cggcctcaa gggggccaa 
        caggggggt tcagggggg tcatctaac ccgattcag 
        tcaattggg agtcagttc gggggccaa gggggcaac 
        gggtcgaat ccgttcgtc tcaggggcg caggccatt 
        ggaagtcag tttgcggga caagggggt aatgggtcg 
        aacccgttt gtctcagga gcgcaagcg attgggagc 
        cagtttggc caggccatt ggggggcaa ggggggcaa 
        ggtgggggc tcgaatccg ttggtttcg ggggcgcag 
        gctattggg aaccagttc cagcaggct attgggcaa 
        ggggggaat aagccggtt gaagggggt gcaggagag 
        gccacctct gagaagacg agagcggag agtttggtg 
        aatgaggga gagaaagtg gagaagatg aaagagaag 
        gttgagaag aaagtggag aaggtggag gaaaaggtt 
        gagaagaag ccagcggaa atgatggcc atggcggag 
        tag
      mRNA sequence
      • >TC010421 RA
        aaacgttca gcaccatgt ggtcaagat gttcggtgg 
        gtttttgtt tgctccttg ctgcccagg cagcggtac 
        tcaaaacga gaaatattc ccgttgaaa ttgtctacg 
        atagaacga acccggcca cgtacggga cgagaaatt 
        tagacgcca aaatacaat tatcatcgg acgaattgc 
        tgaaattga agctcagtg atgatatca tcaacgaag 
        aaaaaattc atttcgaag aaggggatg ctgggaatg 
        aacaagggg tgaaaaact tggaggaga aaattcaac 
        tgtcgtctg aagaaatta gccatcagt cgcaaaatg 
        cggtggaaa ttgacggca atattaaag ctgccgttg 
        cggaaaatt cggcacaat ccgggaatt tgcttgatc 
        ttttgaaac gggctgaag agatggctt acgctggca 
        ttaacacga ttagggaga actttgata aggtctcag 
        atagtatac cgggggcca aaccaacgg cagaagctt 
        gggaaaaat taaaccaaa ctttgcatg actacttca 
        gggatcagt acaagaacg ccctcactt tgaaacagg 
        atcagccaa caagtggta acgaaaacc ccaatcaga 
        atcaaaatc agaaccaat ttttccaaa acttaatca 
        atggtatcc aaaccattt ccaacaact ttatgggca 
        attttggcc aaaatgggc aaacgcaga aaccgcctg 
        ggtccgagg atgaacaac aaccacagc agcaacaac 
        aaggcccgt ggcaaagct tcgtcagct atttccaag 
        gaggaatga accaaatgg taaccaaca tgaacaact 
        atttcaacc gacagaatg gtacctccg ggcaagaag 
        acaccggaa ctgggggta acgggggtt caaacttca 
        tcaacaact ttgtcagtt caatccagc aatttttcc 
        aaggccagg ggggccagg ggggcagcc cctcccaaa 
        caatcaacg tcaacaacc aaagcgacc ccggagtga 
        ccaccaacg cccccggat tttttcagg gcgccatta 
        accaattca accaagtgg ttaacaact tccggcctt 
        cgacctccg cccggcccc cggtgcaag gtgacgaag 
        gctcgtcaa caactccgg ggaatttta tccaaagca 
        taggccagg cgttccaga acgttaaca ttttccggc 
        ctcaagggg gccaacagg ggggttcag gggggtcat 
        ctaacccga ttcagtcaa ttgggagtc agttcgggg 
        gccaagggg gcaacgggt cgaatccgt tcgtctcag 
        gggcgcagg ccattggaa gtcagtttg cgggacaag 
        ggggtaatg ggtcgaacc cgtttgtct caggagcgc 
        aagcgattg ggagccagt ttggccagg ccattgggg 
        ggcaagggg ggcaaggtg ggggctcga atccgttgg 
        tttcggggg cgcaggcta ttgggaacc agttccagc 
        aggctattg ggcaagggg ggaataagc cggttgaag 
        ggggtgcag gagaggcca cctctgaga agacgagag 
        cggagagtt tggtgaatg agggagaga aagtggaga 
        agatgaaag agaaggttg agaagaaag tggagaagg 
        tggaggaaa aggttgaga agaagccag cggaaatga 
        tggccatgg cggagtaga attttaaga gaataatga 
        aatttgaaa tcatcgttt gaaatggat tatgagaat 
        taatttatg attatttat taataaatt attttgtga 
        caa
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