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Gene information
General information
TC010406 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7637034..7648748
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010406
    iBeetle screen iB_04833

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010406 RA
      MEPNEQQEE VKPKPQETT ETEQATTSV IIRRQVITT 
      AGTIEDNIE EVEQIAGEE IVNTSDSPV QQVAVDQEP 
      NVVQIVQYE DQPASEQHY EEGAPSVYV CTTEAYHAQ 
      TEYPQQETV AYTTLDIPA NAQAITTIE TTEYADLES 
      VNNTTYSGQ YASNDATQY IHTQYPADY GNYPGERAG 
      AESPQTALF RNTDPNLAS SRYSQFEVS NQSITNQVV 
      MNANGTYPQ FTTQNPGWP NAGAAEYTY APANVSLHQ 
      ADSTHQQYA NSTWSNTAA VEDSPRGQY REVHIKECV 
      NCGASVTPL WRRDGTGHY LCNACGLYN KINGVNRPP 
      VRPPKKPQA QPGPRRNGV QCANCKTGN TTLWRRNNQ 
      GEPVCNACG LYFKLHNVN RPLSMKKEG IQTRKRRPK 
      SSNSHSQPA PSTSQGLIP ARMVTHHGY YPPNLQEVA 
      SDQYQLPVT NYQINQYQQ RLPGAEQLT RPIPQNVTP 
      LEPIQISQS DEQTSVITS TSQSTRFRH HDDEDDDTN 
      PNA
    • >TC010406 RB
      MEPNEQQEE VKPKPQETT ETEQATTSV IIRRQVITT 
      AGTIEDNIE EVEQIAGEE IVNTSDSPV QQVAVDQEP 
      NVVQIVQYE DQPASEQHY EEGAPSVYV CTTEAYHAQ 
      TEYPQQETV AYTTLDIPA NAQAITTIE TTEYADLES 
      VNNTTYSGQ YASNDATQY IHTQYPADY GNYPGERAG 
      AESPQTALF RNTDPNLAS SRYSQFEVS NQSITNQVV 
      MNANGTYPQ FTTQNPGWP NAGAAEYTY APANVSLHQ 
      ADSTHQQYA NSTWSNTAA VEDSPRGQY REVHIKECV 
      NCGASVTPL WRRDGTGHY LCNACGLYN KINGVNRPP 
      VRPPKKPQA QPGPRRNGV QCANCKTGN TTLWRRNNQ 
      GEPVCNACG LYFKLHNVN RPLSMKKEG IQTRKRRPK 
      SSNSHSQPA PSTSQGLIP ARMVTHHGY YPPNLQEVA 
      SDQYQLPVT NYQINQYQQ RLPGAEQLT RPIPQNFST 
      A
    CDS sequence
    • >TC010406 RA
      atggaacca aacgagcaa caagaagag gtaaagccg 
      aaacctcaa gaaaccacc gaaaccgaa caagccacc 
      acaagtgtc ataatacgc cgccaagtc ataaccacc 
      gctggaaca atcgaagac aacatcgaa gaagttgaa 
      caaatagcc ggcgaagaa atcgtcaac acttccgat 
      tcacctgtg caacaagtg gccgtagat caagaaccg 
      aatgtggtt caaattgta caatatgaa gaccaaccg 
      gcttccgaa caacattac gaagaaggg gcgccatca 
      gtgtacgtg tgcaccacg gaagcatat cacgctcaa 
      accgaatat cctcagcag gaaacggtg gcttacacg 
      acgctcgat attcccgca aacgctcaa gccattaca 
      acgattgag acgactgaa tatgcagat ttggagagt 
      gttaacaat accacatac agtggccaa tatgcaagt 
      aatgacgcc acgcaatat atccataca cagtaccca 
      gctgattat ggaaattat ccaggggag agggctggg 
      gcagaaagc ccacaaacg gcactcttc aggaacacc 
      gacccaaat cttgcctca tccagatat tcgcagttc 
      gaagtgtct aaccaaagc atcactaat caggtggtg 
      atgaatgcc aacgggacg tatccccaa ttcacaacg 
      cagaatcca ggttggccc aacgctgga gctgctgag 
      tacacatat gcacccgca aacgtgtct cttcaccaa 
      gcagacagt actcaccaa cagtacgca aattcgact 
      tggtccaac actgcagcc gttgaggac tcacctaga 
      ggacaatat cgagaggta catatcaaa gaatgcgtg 
      aattgcggt gccagtgtt acccctcta tggcgtaga 
      gatggtacc ggtcactat ctctgtaat gcttgcggt 
      ctctataac aaaattaat ggtgttaat agaccacca 
      gtgaggcct cccaaaaag ccacaggcg caacctgga 
      ccgagaaga aatggggtc caatgtgcc aactgtaaa 
      acggggaac accaccctt tggagaaga aacaatcag 
      ggagagccc gtctgtaac gcctgtgga ctgtatttc 
      aaactccac aatgtcaac cgaccccta agtatgaaa 
      aaagagggc attcaaacc cggaaacga agaccaaaa 
      agctccaac tctcacagt caaccagct ccaagcacc 
      agtcaaggg ttaattcca gctagaatg gttactcac 
      catggatat taccctcca aatttacag gaggttgct 
      tccgatcaa taccaattg cctgtaaca aattatcaa 
      ataaatcag tatcaacaa agactccca ggagctgag 
      caacttaca agaccaatt ccgcaaaac gtaactcca 
      ctggagccg atacaaata tctcaaagc gatgaacaa 
      accagtgtt ataacatcg acatcacaa agcactcgt 
      tttcgccat catgacgac gaagacgac gacactaat 
      ccaaatgct tga
    • >TC010406 RB
      atggaacca aacgagcaa caagaagag gtaaagccg 
      aaacctcaa gaaaccacc gaaaccgaa caagccacc 
      acaagtgtc ataatacgc cgccaagtc ataaccacc 
      gctggaaca atcgaagac aacatcgaa gaagttgaa 
      caaatagcc ggcgaagaa atcgtcaac acttccgat 
      tcacctgtg caacaagtg gccgtagat caagaaccg 
      aatgtggtt caaattgta caatatgaa gaccaaccg 
      gcttccgaa caacattac gaagaaggg gcgccatca 
      gtgtacgtg tgcaccacg gaagcatat cacgctcaa 
      accgaatat cctcagcag gaaacggtg gcttacacg 
      acgctcgat attcccgca aacgctcaa gccattaca 
      acgattgag acgactgaa tatgcagat ttggagagt 
      gttaacaat accacatac agtggccaa tatgcaagt 
      aatgacgcc acgcaatat atccataca cagtaccca 
      gctgattat ggaaattat ccaggggag agggctggg 
      gcagaaagc ccacaaacg gcactcttc aggaacacc 
      gacccaaat cttgcctca tccagatat tcgcagttc 
      gaagtgtct aaccaaagc atcactaat caggtggtg 
      atgaatgcc aacgggacg tatccccaa ttcacaacg 
      cagaatcca ggttggccc aacgctgga gctgctgag 
      tacacatat gcacccgca aacgtgtct cttcaccaa 
      gcagacagt actcaccaa cagtacgca aattcgact 
      tggtccaac actgcagcc gttgaggac tcacctaga 
      ggacaatat cgagaggta catatcaaa gaatgcgtg 
      aattgcggt gccagtgtt acccctcta tggcgtaga 
      gatggtacc ggtcactat ctctgtaat gcttgcggt 
      ctctataac aaaattaat ggtgttaat agaccacca 
      gtgaggcct cccaaaaag ccacaggcg caacctgga 
      ccgagaaga aatggggtc caatgtgcc aactgtaaa 
      acggggaac accaccctt tggagaaga aacaatcag 
      ggagagccc gtctgtaac gcctgtgga ctgtatttc 
      aaactccac aatgtcaac cgaccccta agtatgaaa 
      aaagagggc attcaaacc cggaaacga agaccaaaa 
      agctccaac tctcacagt caaccagct ccaagcacc 
      agtcaaggg ttaattcca gctagaatg gttactcac 
      catggatat taccctcca aatttacag gaggttgct 
      tccgatcaa taccaattg cctgtaaca aattatcaa 
      ataaatcag tatcaacaa agactccca ggagctgag 
      caacttaca agaccaatt ccgcaaaat ttttccaca 
      gcgtaa
    mRNA sequence
    • >TC010406 RA
      catttaact acgccaagt gatcaaaca gtttattgt 
      cctgtgaac acacgttac tgtggcgcg tcattgttt 
      cccccattt ctcatttgg cacctagtc cgccatgga 
      accaaacga gcaacaaga agaggtaaa gccgaaacc 
      tcaagaaac caccgaaac cgaacaagc caccacaag 
      tgtcataat acgccgcca agtcataac caccgctgg 
      aacaatcga agacaacat cgaagaagt tgaacaaat 
      agccggcga agaaatcgt caacacttc cgattcacc 
      tgtgcaaca agtggccgt agatcaaga accgaatgt 
      ggttcaaat tgtacaata tgaagacca accggcttc 
      cgaacaaca ttacgaaga aggggcgcc atcagtgta 
      cgtgtgcac cacggaagc atatcacgc tcaaaccga 
      atatcctca gcaggaaac ggtggctta cacgacgct 
      cgatattcc cgcaaacgc tcaagccat tacaacgat 
      tgagacgac tgaatatgc agatttgga gagtgttaa 
      caataccac atacagtgg ccaatatgc aagtaatga 
      cgccacgca atatatcca tacacagta cccagctga 
      ttatggaaa ttatccagg ggagagggc tggggcaga 
      aagcccaca aacggcact cttcaggaa caccgaccc 
      aaatcttgc ctcatccag atattcgca gttcgaagt 
      gtctaacca aagcatcac taatcaggt ggtgatgaa 
      tgccaacgg gacgtatcc ccaattcac aacgcagaa 
      tccaggttg gcccaacgc tggagctgc tgagtacac 
      atatgcacc cgcaaacgt gtctcttca ccaagcaga 
      cagtactca ccaacagta cgcaaattc gacttggtc 
      caacactgc agccgttga ggactcacc tagaggaca 
      atatcgaga ggtacatat caaagaatg cgtgaattg 
      cggtgccag tgttacccc tctatggcg tagagatgg 
      taccggtca ctatctctg taatgcttg cggtctcta 
      taacaaaat taatggtgt taatagacc accagtgag 
      gcctcccaa aaagccaca ggcgcaacc tggaccgag 
      aagaaatgg ggtccaatg tgccaactg taaaacggg 
      gaacaccac cctttggag aagaaacaa tcagggaga 
      gcccgtctg taacgcctg tggactgta tttcaaact 
      ccacaatgt caaccgacc cctaagtat gaaaaaaga 
      gggcattca aacccggaa acgaagacc aaaaagctc 
      caactctca cagtcaacc agctccaag caccagtca 
      agggttaat tccagctag aatggttac tcaccatgg 
      atattaccc tccaaattt acaggaggt tgcttccga 
      tcaatacca attgcctgt aacaaatta tcaaataaa 
      tcagtatca acaaagact cccaggagc tgagcaact 
      tacaagacc aattccgca aaacgtaac tccactgga 
      gccgataca aatatctca aagcgatga acaaaccag 
      tgttataac atcgacatc acaaagcac tcgttttcg 
      ccatcatga cgacgaaga cgacgacac taatccaaa 
      tgcttgaaa acttgttcc aacacaaac tatttgcga 
      tgtttttgg atacaactg tttcttctt cgtcttgcg 
      tagttttag ccagttttt agaattagt aattgaaaa 
      gatttgcag tacttaatt gaaatacac tataataag 
      tctaaatat ttactaata atctagata aacttgcca 
      ataatattt aataggatt tagttacct gtttgaaga 
      catgtttaa gttttaccg aacaaaagt tctcacttt 
      tttcagata tttctttat ccaaaacga tcgtaaaag 
      tgaatgtat atacacaat acctttcta aaacatgta 
      atattgatt cctagtcac cttctaacg atttaaagt 
      cagacatta ctttaagtt atattttta atatttatg 
      tgtttgaaa ttttactta gttaggttt ttgtttttt 
      gccttattg tccaaagat aacaaaaaa aaatttatt 
      acattaggt atattttac gctgtgata attcgaatt 
      tttttagta acttatttt gccattttt cttatgtat 
      ctggataat gtgccatta taaatagtt actaaatta 
      taaagggta gt
    • >TC010406 RB
      cctatcgat gcctagatt caatatggc ggcttcctc 
      tctttttcg ctttcatct aattagctg agttttatt 
      aattgttgt aaagacacc taataagaa acatcatct 
      agtacagcg tttttaaat atttttcaa ctgcaaaga 
      acactgtgg tctctctaa cacttttaa cgagaaatt 
      ggtcattgt ttcccccat ttctcattt ggcacctag 
      tccgccatg gaaccaaac gagcaacaa gaagaggta 
      aagccgaaa cctcaagaa accaccgaa accgaacaa 
      gccaccaca agtgtcata atacgccgc caagtcata 
      accaccgct ggaacaatc gaagacaac atcgaagaa 
      gttgaacaa atagccggc gaagaaatc gtcaacact 
      tccgattca cctgtgcaa caagtggcc gtagatcaa 
      gaaccgaat gtggttcaa attgtacaa tatgaagac 
      caaccggct tccgaacaa cattacgaa gaaggggcg 
      ccatcagtg tacgtgtgc accacggaa gcatatcac 
      gctcaaacc gaatatcct cagcaggaa acggtggct 
      tacacgacg ctcgatatt cccgcaaac gctcaagcc 
      attacaacg attgagacg actgaatat gcagatttg 
      gagagtgtt aacaatacc acatacagt ggccaatat 
      gcaagtaat gacgccacg caatatatc catacacag 
      tacccagct gattatgga aattatcca ggggagagg 
      gctggggca gaaagccca caaacggca ctcttcagg 
      aacaccgac ccaaatctt gcctcatcc agatattcg 
      cagttcgaa gtgtctaac caaagcatc actaatcag 
      gtggtgatg aatgccaac gggacgtat ccccaattc 
      acaacgcag aatccaggt tggcccaac gctggagct 
      gctgagtac acatatgca cccgcaaac gtgtctctt 
      caccaagca gacagtact caccaacag tacgcaaat 
      tcgacttgg tccaacact gcagccgtt gaggactca 
      cctagagga caatatcga gaggtacat atcaaagaa 
      tgcgtgaat tgcggtgcc agtgttacc cctctatgg 
      cgtagagat ggtaccggt cactatctc tgtaatgct 
      tgcggtctc tataacaaa attaatggt gttaataga 
      ccaccagtg aggcctccc aaaaagcca caggcgcaa 
      cctggaccg agaagaaat ggggtccaa tgtgccaac 
      tgtaaaacg gggaacacc accctttgg agaagaaac 
      aatcaggga gagcccgtc tgtaacgcc tgtggactg 
      tatttcaaa ctccacaat gtcaaccga cccctaagt 
      atgaaaaaa gagggcatt caaacccgg aaacgaaga 
      ccaaaaagc tccaactct cacagtcaa ccagctcca 
      agcaccagt caagggtta attccagct agaatggtt 
      actcaccat ggatattac cctccaaat ttacaggag 
      gttgcttcc gatcaatac caattgcct gtaacaaat 
      tatcaaata aatcagtat caacaaaga ctcccagga 
      gctgagcaa cttacaaga ccaattccg caaaatttt 
      tccacagcg taactccac tggagccga tacaaatat 
      ctcaaagcg atgaacaaa ccagtgtta taacatcga 
      catcacaaa gcactcgtt ttcgccatc atgacgacg 
      aagacgacg acactaatc caaatgctt gaaaacttg 
      ttccaacac aaactattt gcgatgttt ttggataca 
      actgtttct tcttcgtct tgcgtagtt ttagccagt 
      ttttagaat tagtaattg aaaagattt gcagtactt 
      aattgaaat acactataa taagtctaa atatttact 
      aataatcta gataaactt gccaataat atttaatag 
      gatttagtt acctgtttg aagacatgt ttaagtttt 
      accgaacaa aagttctca cttttttca gatatttct 
      ttatccaaa acgatcgta aaagtgaat gtatataca 
      caatacctt tctaaaaca tgtaatatt gattcctag 
      tcaccttct aacgattta aagtcagac attacttta 
      agttatatt tttaatatt tatgtgttt gaaatttta 
      cttagttag gtttttgtt ttttgcctt attgtccaa 
      agataacaa aaaaaaatt tattacatt aggtatatt 
      ttacgctgt gataattcg aattttttt agtaactta 
      ttttgccat ttttcttat gtatctgga taatgtgcc 
      attataaat agttactaa attataaag ggtagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY