| General information | |||
| TC010406 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7637034..7648748 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010406 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04833 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC010406 RA
MEPNEQQEE VKPKPQETT ETEQATTSV IIRRQVITT AGTIEDNIE EVEQIAGEE IVNTSDSPV QQVAVDQEP NVVQIVQYE DQPASEQHY EEGAPSVYV CTTEAYHAQ TEYPQQETV AYTTLDIPA NAQAITTIE TTEYADLES VNNTTYSGQ YASNDATQY IHTQYPADY GNYPGERAG AESPQTALF RNTDPNLAS SRYSQFEVS NQSITNQVV MNANGTYPQ FTTQNPGWP NAGAAEYTY APANVSLHQ ADSTHQQYA NSTWSNTAA VEDSPRGQY REVHIKECV NCGASVTPL WRRDGTGHY LCNACGLYN KINGVNRPP VRPPKKPQA QPGPRRNGV QCANCKTGN TTLWRRNNQ GEPVCNACG LYFKLHNVN RPLSMKKEG IQTRKRRPK SSNSHSQPA PSTSQGLIP ARMVTHHGY YPPNLQEVA SDQYQLPVT NYQINQYQQ RLPGAEQLT RPIPQNVTP LEPIQISQS DEQTSVITS TSQSTRFRH HDDEDDDTN PNA
- >TC010406 RB
MEPNEQQEE VKPKPQETT ETEQATTSV IIRRQVITT AGTIEDNIE EVEQIAGEE IVNTSDSPV QQVAVDQEP NVVQIVQYE DQPASEQHY EEGAPSVYV CTTEAYHAQ TEYPQQETV AYTTLDIPA NAQAITTIE TTEYADLES VNNTTYSGQ YASNDATQY IHTQYPADY GNYPGERAG AESPQTALF RNTDPNLAS SRYSQFEVS NQSITNQVV MNANGTYPQ FTTQNPGWP NAGAAEYTY APANVSLHQ ADSTHQQYA NSTWSNTAA VEDSPRGQY REVHIKECV NCGASVTPL WRRDGTGHY LCNACGLYN KINGVNRPP VRPPKKPQA QPGPRRNGV QCANCKTGN TTLWRRNNQ GEPVCNACG LYFKLHNVN RPLSMKKEG IQTRKRRPK SSNSHSQPA PSTSQGLIP ARMVTHHGY YPPNLQEVA SDQYQLPVT NYQINQYQQ RLPGAEQLT RPIPQNFST A
- >TC010406 RA
atggaacca aacgagcaa caagaagag gtaaagccg aaacctcaa gaaaccacc gaaaccgaa caagccacc acaagtgtc ataatacgc cgccaagtc ataaccacc gctggaaca atcgaagac aacatcgaa gaagttgaa caaatagcc ggcgaagaa atcgtcaac acttccgat tcacctgtg caacaagtg gccgtagat caagaaccg aatgtggtt caaattgta caatatgaa gaccaaccg gcttccgaa caacattac gaagaaggg gcgccatca gtgtacgtg tgcaccacg gaagcatat cacgctcaa accgaatat cctcagcag gaaacggtg gcttacacg acgctcgat attcccgca aacgctcaa gccattaca acgattgag acgactgaa tatgcagat ttggagagt gttaacaat accacatac agtggccaa tatgcaagt aatgacgcc acgcaatat atccataca cagtaccca gctgattat ggaaattat ccaggggag agggctggg gcagaaagc ccacaaacg gcactcttc aggaacacc gacccaaat cttgcctca tccagatat tcgcagttc gaagtgtct aaccaaagc atcactaat caggtggtg atgaatgcc aacgggacg tatccccaa ttcacaacg cagaatcca ggttggccc aacgctgga gctgctgag tacacatat gcacccgca aacgtgtct cttcaccaa gcagacagt actcaccaa cagtacgca aattcgact tggtccaac actgcagcc gttgaggac tcacctaga ggacaatat cgagaggta catatcaaa gaatgcgtg aattgcggt gccagtgtt acccctcta tggcgtaga gatggtacc ggtcactat ctctgtaat gcttgcggt ctctataac aaaattaat ggtgttaat agaccacca gtgaggcct cccaaaaag ccacaggcg caacctgga ccgagaaga aatggggtc caatgtgcc aactgtaaa acggggaac accaccctt tggagaaga aacaatcag ggagagccc gtctgtaac gcctgtgga ctgtatttc aaactccac aatgtcaac cgaccccta agtatgaaa aaagagggc attcaaacc cggaaacga agaccaaaa agctccaac tctcacagt caaccagct ccaagcacc agtcaaggg ttaattcca gctagaatg gttactcac catggatat taccctcca aatttacag gaggttgct tccgatcaa taccaattg cctgtaaca aattatcaa ataaatcag tatcaacaa agactccca ggagctgag caacttaca agaccaatt ccgcaaaac gtaactcca ctggagccg atacaaata tctcaaagc gatgaacaa accagtgtt ataacatcg acatcacaa agcactcgt tttcgccat catgacgac gaagacgac gacactaat ccaaatgct tga
- >TC010406 RB
atggaacca aacgagcaa caagaagag gtaaagccg aaacctcaa gaaaccacc gaaaccgaa caagccacc acaagtgtc ataatacgc cgccaagtc ataaccacc gctggaaca atcgaagac aacatcgaa gaagttgaa caaatagcc ggcgaagaa atcgtcaac acttccgat tcacctgtg caacaagtg gccgtagat caagaaccg aatgtggtt caaattgta caatatgaa gaccaaccg gcttccgaa caacattac gaagaaggg gcgccatca gtgtacgtg tgcaccacg gaagcatat cacgctcaa accgaatat cctcagcag gaaacggtg gcttacacg acgctcgat attcccgca aacgctcaa gccattaca acgattgag acgactgaa tatgcagat ttggagagt gttaacaat accacatac agtggccaa tatgcaagt aatgacgcc acgcaatat atccataca cagtaccca gctgattat ggaaattat ccaggggag agggctggg gcagaaagc ccacaaacg gcactcttc aggaacacc gacccaaat cttgcctca tccagatat tcgcagttc gaagtgtct aaccaaagc atcactaat caggtggtg atgaatgcc aacgggacg tatccccaa ttcacaacg cagaatcca ggttggccc aacgctgga gctgctgag tacacatat gcacccgca aacgtgtct cttcaccaa gcagacagt actcaccaa cagtacgca aattcgact tggtccaac actgcagcc gttgaggac tcacctaga ggacaatat cgagaggta catatcaaa gaatgcgtg aattgcggt gccagtgtt acccctcta tggcgtaga gatggtacc ggtcactat ctctgtaat gcttgcggt ctctataac aaaattaat ggtgttaat agaccacca gtgaggcct cccaaaaag ccacaggcg caacctgga ccgagaaga aatggggtc caatgtgcc aactgtaaa acggggaac accaccctt tggagaaga aacaatcag ggagagccc gtctgtaac gcctgtgga ctgtatttc aaactccac aatgtcaac cgaccccta agtatgaaa aaagagggc attcaaacc cggaaacga agaccaaaa agctccaac tctcacagt caaccagct ccaagcacc agtcaaggg ttaattcca gctagaatg gttactcac catggatat taccctcca aatttacag gaggttgct tccgatcaa taccaattg cctgtaaca aattatcaa ataaatcag tatcaacaa agactccca ggagctgag caacttaca agaccaatt ccgcaaaat ttttccaca gcgtaa
- >TC010406 RA
catttaact acgccaagt gatcaaaca gtttattgt cctgtgaac acacgttac tgtggcgcg tcattgttt cccccattt ctcatttgg cacctagtc cgccatgga accaaacga gcaacaaga agaggtaaa gccgaaacc tcaagaaac caccgaaac cgaacaagc caccacaag tgtcataat acgccgcca agtcataac caccgctgg aacaatcga agacaacat cgaagaagt tgaacaaat agccggcga agaaatcgt caacacttc cgattcacc tgtgcaaca agtggccgt agatcaaga accgaatgt ggttcaaat tgtacaata tgaagacca accggcttc cgaacaaca ttacgaaga aggggcgcc atcagtgta cgtgtgcac cacggaagc atatcacgc tcaaaccga atatcctca gcaggaaac ggtggctta cacgacgct cgatattcc cgcaaacgc tcaagccat tacaacgat tgagacgac tgaatatgc agatttgga gagtgttaa caataccac atacagtgg ccaatatgc aagtaatga cgccacgca atatatcca tacacagta cccagctga ttatggaaa ttatccagg ggagagggc tggggcaga aagcccaca aacggcact cttcaggaa caccgaccc aaatcttgc ctcatccag atattcgca gttcgaagt gtctaacca aagcatcac taatcaggt ggtgatgaa tgccaacgg gacgtatcc ccaattcac aacgcagaa tccaggttg gcccaacgc tggagctgc tgagtacac atatgcacc cgcaaacgt gtctcttca ccaagcaga cagtactca ccaacagta cgcaaattc gacttggtc caacactgc agccgttga ggactcacc tagaggaca atatcgaga ggtacatat caaagaatg cgtgaattg cggtgccag tgttacccc tctatggcg tagagatgg taccggtca ctatctctg taatgcttg cggtctcta taacaaaat taatggtgt taatagacc accagtgag gcctcccaa aaagccaca ggcgcaacc tggaccgag aagaaatgg ggtccaatg tgccaactg taaaacggg gaacaccac cctttggag aagaaacaa tcagggaga gcccgtctg taacgcctg tggactgta tttcaaact ccacaatgt caaccgacc cctaagtat gaaaaaaga gggcattca aacccggaa acgaagacc aaaaagctc caactctca cagtcaacc agctccaag caccagtca agggttaat tccagctag aatggttac tcaccatgg atattaccc tccaaattt acaggaggt tgcttccga tcaatacca attgcctgt aacaaatta tcaaataaa tcagtatca acaaagact cccaggagc tgagcaact tacaagacc aattccgca aaacgtaac tccactgga gccgataca aatatctca aagcgatga acaaaccag tgttataac atcgacatc acaaagcac tcgttttcg ccatcatga cgacgaaga cgacgacac taatccaaa tgcttgaaa acttgttcc aacacaaac tatttgcga tgtttttgg atacaactg tttcttctt cgtcttgcg tagttttag ccagttttt agaattagt aattgaaaa gatttgcag tacttaatt gaaatacac tataataag tctaaatat ttactaata atctagata aacttgcca ataatattt aataggatt tagttacct gtttgaaga catgtttaa gttttaccg aacaaaagt tctcacttt tttcagata tttctttat ccaaaacga tcgtaaaag tgaatgtat atacacaat acctttcta aaacatgta atattgatt cctagtcac cttctaacg atttaaagt cagacatta ctttaagtt atattttta atatttatg tgtttgaaa ttttactta gttaggttt ttgtttttt gccttattg tccaaagat aacaaaaaa aaatttatt acattaggt atattttac gctgtgata attcgaatt tttttagta acttatttt gccattttt cttatgtat ctggataat gtgccatta taaatagtt actaaatta taaagggta gt
- >TC010406 RB
cctatcgat gcctagatt caatatggc ggcttcctc tctttttcg ctttcatct aattagctg agttttatt aattgttgt aaagacacc taataagaa acatcatct agtacagcg tttttaaat atttttcaa ctgcaaaga acactgtgg tctctctaa cacttttaa cgagaaatt ggtcattgt ttcccccat ttctcattt ggcacctag tccgccatg gaaccaaac gagcaacaa gaagaggta aagccgaaa cctcaagaa accaccgaa accgaacaa gccaccaca agtgtcata atacgccgc caagtcata accaccgct ggaacaatc gaagacaac atcgaagaa gttgaacaa atagccggc gaagaaatc gtcaacact tccgattca cctgtgcaa caagtggcc gtagatcaa gaaccgaat gtggttcaa attgtacaa tatgaagac caaccggct tccgaacaa cattacgaa gaaggggcg ccatcagtg tacgtgtgc accacggaa gcatatcac gctcaaacc gaatatcct cagcaggaa acggtggct tacacgacg ctcgatatt cccgcaaac gctcaagcc attacaacg attgagacg actgaatat gcagatttg gagagtgtt aacaatacc acatacagt ggccaatat gcaagtaat gacgccacg caatatatc catacacag tacccagct gattatgga aattatcca ggggagagg gctggggca gaaagccca caaacggca ctcttcagg aacaccgac ccaaatctt gcctcatcc agatattcg cagttcgaa gtgtctaac caaagcatc actaatcag gtggtgatg aatgccaac gggacgtat ccccaattc acaacgcag aatccaggt tggcccaac gctggagct gctgagtac acatatgca cccgcaaac gtgtctctt caccaagca gacagtact caccaacag tacgcaaat tcgacttgg tccaacact gcagccgtt gaggactca cctagagga caatatcga gaggtacat atcaaagaa tgcgtgaat tgcggtgcc agtgttacc cctctatgg cgtagagat ggtaccggt cactatctc tgtaatgct tgcggtctc tataacaaa attaatggt gttaataga ccaccagtg aggcctccc aaaaagcca caggcgcaa cctggaccg agaagaaat ggggtccaa tgtgccaac tgtaaaacg gggaacacc accctttgg agaagaaac aatcaggga gagcccgtc tgtaacgcc tgtggactg tatttcaaa ctccacaat gtcaaccga cccctaagt atgaaaaaa gagggcatt caaacccgg aaacgaaga ccaaaaagc tccaactct cacagtcaa ccagctcca agcaccagt caagggtta attccagct agaatggtt actcaccat ggatattac cctccaaat ttacaggag gttgcttcc gatcaatac caattgcct gtaacaaat tatcaaata aatcagtat caacaaaga ctcccagga gctgagcaa cttacaaga ccaattccg caaaatttt tccacagcg taactccac tggagccga tacaaatat ctcaaagcg atgaacaaa ccagtgtta taacatcga catcacaaa gcactcgtt ttcgccatc atgacgacg aagacgacg acactaatc caaatgctt gaaaacttg ttccaacac aaactattt gcgatgttt ttggataca actgtttct tcttcgtct tgcgtagtt ttagccagt ttttagaat tagtaattg aaaagattt gcagtactt aattgaaat acactataa taagtctaa atatttact aataatcta gataaactt gccaataat atttaatag gatttagtt acctgtttg aagacatgt ttaagtttt accgaacaa aagttctca cttttttca gatatttct ttatccaaa acgatcgta aaagtgaat gtatataca caatacctt tctaaaaca tgtaatatt gattcctag tcaccttct aacgattta aagtcagac attacttta agttatatt tttaatatt tatgtgttt gaaatttta cttagttag gtttttgtt ttttgcctt attgtccaa agataacaa aaaaaaatt tattacatt aggtatatt ttacgctgt gataattcg aattttttt agtaactta ttttgccat ttttcttat gtatctgga taatgtgcc attataaat agttactaa attataaag ggtagt
