| General information | |||
| TC010404 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7684770..7692697 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010404 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04832 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Days post injection
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
Days post injection
larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
Days post injection
- >TC010404 RA
MDARIQPQS SICSDSNWE EPAGNDVAP LKSNRRICL VGGLTTDTA LRTVAESFR VPVVTSKDG EDCAQDDSF STVFILESF EGDVFNCLY KQKQSLMGP LALQQLANK NEPLPDNTR PLFNLAMTG VVVCFTGFR NKSDLATLV PLIHHMGGS IRKNISQKV THLIANICN GEKYRYAAV FRVPVMNQD WVYASWKKR HDINFSATV DAFVAEFKL KPFHGAKVC FVGFAEDEK KHMTDILLS NGGTVTSLE DPQCSHVVM ERSEVCTLE AASAPLSPT EPVNPSIPD PPEHLNYNG FLETLHEKS RDSCIDDSN LNTSEFSSG SRKRKIPST ESSVVKTKR RKDKCASER KSKVSGIFK TPLNYLSNR RRTIDTFSF NETLNQSVV SSSGVFNVD TVENLSVLN QSQFTPQSS NKQKKNLFA RTFSSSKFN RSKLKKCNL NATRLSFSE ASDVDGNEP LNASCFPNI SLNPVPHSE LRSDCSKDA RGPSVPTIS VVDESSVAE RPETTSPKV FLVKAEWFW TSVQKEICL DEKDYLYDD YVEQTFSPT RRESHQATP SSASRRKRK RLHEAVLSL THQQSPGLH KRRSSVSDA GLLSVSGSF LDCTASPDK ALTDGTEPS IDTPRKGLT ARQQVFLEL VQTESNYVD ILHTIMTMF KKHLEEMPE EEALLNNTE LNLIFGKVP AIYETHMKM RDELRCFAS TWSEDHTIG SIIVKYSKD LLKAYPPYI NYFEEMKEV LTKCDQNKP RFHAFLKAC QTKPECGRQ SLQELMIRP VQRLGSICL LLANLLKHT PKSNPDHAE LQRALDGLK EGMDLINED KRKAEAQVA LFNMFNDID NCPPDIVCS HRKFIAKAE VIQVSTTEG LSSKGNSLV LFLFSDLVE VCKKKSKTF NNAKSPTGS VNSLQRPKL YKHVKLLPL NTIKKVIDI KETEQCQKV FCFVCRNND ELKERVYSF AMPDEDADK AGFLKTLTK QMANNACTP DADKFLAYL ESHQLDIDT SDMNKGTLN KAFKFASRT KLKVGRAFS FNKTPSKLK RAVSSMMSP FGSSTDYLH TT
- >TC010404 RB
MDARIQPQS SICSDSNWE EPAGNDVAP LKSNRRICL VGGLTTDTA LRTVAESFR VPVVTSKDG EDCAQDDSF STVFILESF EGDVFNCLY KQKQSLMGP LALQQLANK NEPLPDNTR PLFNLAMTG VVVCFTGFR NKSDLATLV PLIHHMGGS IRKNISQKV THLIANICN GEKYRYAAV FRVPVMNQD WVYASWKKR HDINFSATV DAFVAEFKL KPFHGAKVC FVGFAEDEK KHMTDILLS NGGTVTSLE DPQCSHVVM ERSEVCTLE AASAPLSPT EPVNPSIPD PPEHLNYNG FLETLHEKS RDSCIDDSN LNTSEFSSG SRKRKIPST ESSVVKTKR RKDKCASER KSKVSGIFK TPLNYLSNR RRTIDTFSF NETLNQSVV SSSGVFNVD TVENLSVLN QSQFTPQSS NKQKKNLFA RTFSSSKFN RSKLKKCNL NATRLSFSE ASDVDGNEP LNASCFPNI SLNPVPHSE LRSDCSKDA RGPSVPTIS VVDESSVAE RPETTSPKV FLVKAEWFW TSVQKEICL DEKDYLYDD YVEQTFSPT RRESHQATP SSASRRKRK RLHEAVLSL THQQSPDCT ASPDKALTD GTEPSIDTP RKGLTARQQ VFLELVQTE SNYVDILHT IMTMFKKHL EEMPEEEAL LNNTELNLI FGKVPAIYE THMKMRDEL RCFASTWSE DHTIGSIIV KYSKDLLKA YPPYINYFE EMKEVLTKC DQNKPRFHA FLKACQTKP ECGRQSLQE LMIRPVQRL GSICLLLAN LLKHTPKSN PDHAELQRA LDGLKEGMD LINEDKRKA EAQVALFNM FNDIDNCPP DIVCSHRKF IAKAEVIQV STTEGLSSK GNSLVLFLF SDLVEVCKK KSKTFNNAK SPTGSVNSL QRPKLYKHV KLLPLNTIK KVIDIKETE QCQKVFCFV CRNNDELKE RVYSFAMPD EDADKAGFL KTLTKQMAN NACTPDADK FLAYLESHQ LDIDTSDMN KGTLNKAFK FASRTKLKV GRAFSFNKT PSKLKRAVS SMMSPFGSS TDYLHTT
- >TC010404 RC
MDARIQPQS SICSDSNWE EPAGNDVAP LKSNRRICL VGGLTTDTA LRTVAESFR VPVVTSKDG EDCAQDDSF STVFILESF EGDVFNCLY KQKQSLMGP LALQQLANK NEPLPDNTR PLFNLAMTG VVVCFTGFR NKSDLATLV PLIHHMGGS IRKNISQKV THLIANICN GEKYRYAAV FRVPVMNQD WVYASWKKR HDINFSATV DAFVAEFKL KPFHGAKVC FVGFAEDEK KHMTDILLS NGGTVTSLE DPQCSHVVV DESSVAERP ETTSPKVFL VKAEWFWTS VQKEICLDE KDYLYDDYV EQTFSPTRR ESHQATPSS ASRRKRKRL HEAVLSLTH QQSPGLHKR RSSVSDAGL LSVSGSFLD CTASPDKAL TDGTEPSID TPRKGLTAR QQVFLELVQ TESNYVDIL HTIMTMFKK HLEEMPEEE ALLNNTELN LIFGKVPAI YETHMKMRD ELRCFASTW SEDHTIGSI IVKYSKDLL KAYPPYINY FEEMKEVLT KCDQNKPRF HAFLKACQT KPECGRQSL QELMIRPVQ RLGSICLLL ANLLKHTPK SNPDHAELQ RALDGLKEG MDLINEDKR KAEAQVALF NMFNDIDNC PPDIVCSHR KFIAKAEVI QVSTTEGLS SKGNSLVLF LFSDLVEVC KKKSKTFNN AKSPTGSVN SLQRPKLYK HVKLLPLNT IKKVIDIKE TEQCQKVFC FVCRNNDEL KERVYSFAM PDEDADKAG FLKTLTKQM ANNACTPDA DKFLAYLES HQLDIDTSD MNKGTLNKA FKFASRTKL KVGRAFSFN KTPSKLKRA VSSMMSPFG SSTDYLHTT
- >TC010404 RA
atggacgca cgcattcag ccccaaagt tccatttgt agtgactca aattgggag gaacccgcg ggaaatgac gttgcaccc ttaaagagc aaccgaagg atatgtctg gttgggggt ctgaccacc gatacagcc cttcggacc gtcgcagag tcctttcgg gtgccggtt gttacttca aaagacgga gaagattgt gctcaagat gatagtttt agtaccgtt tttatatta gagagtttc gaaggagac gtatttaat tgtttatat aaacagaag cagtccctt atggggccc ttggctctg cagcagcta gccaacaag aatgagcca cttccagac aacacgagg cccctcttc aacctggct atgacgggg gtcgttgtc tgctttact ggattcagg aacaaaagt gatctggca actttggta ccactgatc catcatatg ggtggttcc atcaggaaa aacataagc cagaaagtg acgcattta attgccaac atttgtaat ggagaaaaa tatcgttat gcggccgta tttcgagta cctgtgatg aatcaagat tgggtctat gctagttgg aagaaaaga catgatatt aattttagt gcaacggtt gatgcattt gtggctgaa ttcaagttg aagcctttc catggtgct aaagtgtgt ttcgttggg tttgcagaa gatgagaaa aagcacatg actgatatt ttgttgtcc aatggcggg actgtgact agcttagag gatccccaa tgtagccat gtggtaatg gaacgttca gaagtctgc acacttgaa gccgctagt gcaccgctt tctcccact gaacccgtc aatccctca atccctgac cctcccgag catctaaat tacaacggt tttctcgaa acgttacac gaaaaatcc cgcgacagt tgcattgac gactcaaac ttgaacacc tccgagttt tcttcaggc agtcgtaag cggaaaatc ccgtcgacc gaatcttcc gtcgtgaaa acaaagcga agaaaagat aaatgtgcc agtgagcgg aaaagcaaa gtttcgggc attttcaaa acaccgctt aactattta tcgaaccgt cgacgtaca atcgacact ttcagtttt aacgaaacg ctaaatcag agtgtcgtt tccagctcg ggtgtgttt aatgttgat acggttgag aatttaagc gttttgaat cagagtcaa tttacgcca cagtcgagc aataaacag aaaaagaat ttgtttgcg cgcactttt agttcgtcc aagttcaat cgcagcaag ttgaagaaa tgtaatttg aatgcgacg aggctctcg tttagtgaa gcctccgat gttgatgga aatgaacct ctcaatgct agctgtttc ccgaatatt tcccttaat cctgtacca catagtgag cttcgcagt gattgttcc aaggacgca cgtggacct tctgtgcct actatatcg gtcgtggat gagtcctcg gtggccgaa agacctgaa acaacgtca cctaaagtt ttcctagtg aaagctgaa tggttctgg acctcagta caaaaggag atatgtctt gacgagaaa gactacctc tatgacgac tatgtcgaa cagacgttt tcgccgact cgaagagaa tcgcatcaa gcaacgcca agtagcgcc agtagacgg aaacgtaaa cggttgcac gaagctgtt ttgtcgctg acacatcag cagtcacca ggtttgcat aagcgacgc tcttcagtg agtgatgca ggactgttg tcagtgtct ggttctttc ctagactgc accgccagt ccagataaa gctttaacc gatggcacg gaaccgtcc attgacacg cctaggaaa gggctgact gccaggcag caggttttc ctggaattg gtccaaacg gagtccaat tatgtcgat atcctgcat accatcatg accatgttc aagaaacat ttagaagag atgccggag gaggaagct ttgctcaac aacaccgaa ctgaactta atttttggc aaagtgcca gcgatatac gagacacac atgaaaatg cgcgacgag ttgcgatgt tttgcatca acttggtcc gaagaccac acgataggc agtatcatt gtgaaatat tcgaaagat ctgctcaag gcctaccca ccttacatt aattacttc gaagaaatg aaggaggtt ttaacgaaa tgtgatcaa aacaagcca cgatttcac gcgttcctg aaggcgtgt cagacaaaa ccggaatgt ggcagacag agtttgcag gagttgatg atacggcct gtgcaacga ctggggagc atttgtttg ttgcttgca aatctcctg aaacatacg ccgaaatct aatccggac catgccgaa ctccagcga gctctggac ggtttgaag gaaggcatg gacctgata aacgaagac aagcgaaaa gctgaagcc caagtggcc cttttcaac atgtttaac gacattgat aactgccca ccggacatt gtgtgctcc caccggaag ttcatagca aaggcggaa gtgatccaa gtgtcaaca actgaaggg ctttcgagc aaaggcaac tccttggtc ctcttcctc ttttccgat ttggtagaa gtgtgtaaa aagaagtca aagacgttt aataacgcc aaaagcccg accggcagt gtcaacagt ttgcaaagg ccgaagttg tacaaacac gtcaagtta ctgccattg aacacgatc aagaaagtc atagatatc aaagaaacc gagcagtgc cagaaagtg ttctgtttc gtttgtcgg aataacgac gaattgaaa gagagagtg tattcgttt gctatgccg gacgaggat gccgataaa gcagggttc ctgaaaacc ttgacgaaa caaatggcc aataacgcc tgcacacct gatgcggat aagtttctg gcttatctc gagtcgcat cagttggat atagacact agtgatatg aacaaagga acgttgaat aaggcgttc aaattcgcg tcaaggacg aagttgaag gtgggacgg gccttctcg tttaacaaa acgccgtcg aagttgaaa cgggcggtt tcttcaatg atgtctcca tttggcagc tcaacagac tacctacac acgacatag
- >TC010404 RB
atggacgca cgcattcag ccccaaagt tccatttgt agtgactca aattgggag gaacccgcg ggaaatgac gttgcaccc ttaaagagc aaccgaagg atatgtctg gttgggggt ctgaccacc gatacagcc cttcggacc gtcgcagag tcctttcgg gtgccggtt gttacttca aaagacgga gaagattgt gctcaagat gatagtttt agtaccgtt tttatatta gagagtttc gaaggagac gtatttaat tgtttatat aaacagaag cagtccctt atggggccc ttggctctg cagcagcta gccaacaag aatgagcca cttccagac aacacgagg cccctcttc aacctggct atgacgggg gtcgttgtc tgctttact ggattcagg aacaaaagt gatctggca actttggta ccactgatc catcatatg ggtggttcc atcaggaaa aacataagc cagaaagtg acgcattta attgccaac atttgtaat ggagaaaaa tatcgttat gcggccgta tttcgagta cctgtgatg aatcaagat tgggtctat gctagttgg aagaaaaga catgatatt aattttagt gcaacggtt gatgcattt gtggctgaa ttcaagttg aagcctttc catggtgct aaagtgtgt ttcgttggg tttgcagaa gatgagaaa aagcacatg actgatatt ttgttgtcc aatggcggg actgtgact agcttagag gatccccaa tgtagccat gtggtaatg gaacgttca gaagtctgc acacttgaa gccgctagt gcaccgctt tctcccact gaacccgtc aatccctca atccctgac cctcccgag catctaaat tacaacggt tttctcgaa acgttacac gaaaaatcc cgcgacagt tgcattgac gactcaaac ttgaacacc tccgagttt tcttcaggc agtcgtaag cggaaaatc ccgtcgacc gaatcttcc gtcgtgaaa acaaagcga agaaaagat aaatgtgcc agtgagcgg aaaagcaaa gtttcgggc attttcaaa acaccgctt aactattta tcgaaccgt cgacgtaca atcgacact ttcagtttt aacgaaacg ctaaatcag agtgtcgtt tccagctcg ggtgtgttt aatgttgat acggttgag aatttaagc gttttgaat cagagtcaa tttacgcca cagtcgagc aataaacag aaaaagaat ttgtttgcg cgcactttt agttcgtcc aagttcaat cgcagcaag ttgaagaaa tgtaatttg aatgcgacg aggctctcg tttagtgaa gcctccgat gttgatgga aatgaacct ctcaatgct agctgtttc ccgaatatt tcccttaat cctgtacca catagtgag cttcgcagt gattgttcc aaggacgca cgtggacct tctgtgcct actatatcg gtcgtggat gagtcctcg gtggccgaa agacctgaa acaacgtca cctaaagtt ttcctagtg aaagctgaa tggttctgg acctcagta caaaaggag atatgtctt gacgagaaa gactacctc tatgacgac tatgtcgaa cagacgttt tcgccgact cgaagagaa tcgcatcaa gcaacgcca agtagcgcc agtagacgg aaacgtaaa cggttgcac gaagctgtt ttgtcgctg acacatcag cagtcacca gactgcacc gccagtcca gataaagct ttaaccgat ggcacggaa ccgtccatt gacacgcct aggaaaggg ctgactgcc aggcagcag gttttcctg gaattggtc caaacggag tccaattat gtcgatatc ctgcatacc atcatgacc atgttcaag aaacattta gaagagatg ccggaggag gaagctttg ctcaacaac accgaactg aacttaatt tttggcaaa gtgccagcg atatacgag acacacatg aaaatgcgc gacgagttg cgatgtttt gcatcaact tggtccgaa gaccacacg ataggcagt atcattgtg aaatattcg aaagatctg ctcaaggcc tacccacct tacattaat tacttcgaa gaaatgaag gaggtttta acgaaatgt gatcaaaac aagccacga tttcacgcg ttcctgaag gcgtgtcag acaaaaccg gaatgtggc agacagagt ttgcaggag ttgatgata cggcctgtg caacgactg gggagcatt tgtttgttg cttgcaaat ctcctgaaa catacgccg aaatctaat ccggaccat gccgaactc cagcgagct ctggacggt ttgaaggaa ggcatggac ctgataaac gaagacaag cgaaaagct gaagcccaa gtggccctt ttcaacatg tttaacgac attgataac tgcccaccg gacattgtg tgctcccac cggaagttc atagcaaag gcggaagtg atccaagtg tcaacaact gaagggctt tcgagcaaa ggcaactcc ttggtcctc ttcctcttt tccgatttg gtagaagtg tgtaaaaag aagtcaaag acgtttaat aacgccaaa agcccgacc ggcagtgtc aacagtttg caaaggccg aagttgtac aaacacgtc aagttactg ccattgaac acgatcaag aaagtcata gatatcaaa gaaaccgag cagtgccag aaagtgttc tgtttcgtt tgtcggaat aacgacgaa ttgaaagag agagtgtat tcgtttgct atgccggac gaggatgcc gataaagca gggttcctg aaaaccttg acgaaacaa atggccaat aacgcctgc acacctgat gcggataag tttctggct tatctcgag tcgcatcag ttggatata gacactagt gatatgaac aaaggaacg ttgaataag gcgttcaaa ttcgcgtca aggacgaag ttgaaggtg ggacgggcc ttctcgttt aacaaaacg ccgtcgaag ttgaaacgg gcggtttct tcaatgatg tctccattt ggcagctca acagactac ctacacacg acatag
- >TC010404 RC
atggacgca cgcattcag ccccaaagt tccatttgt agtgactca aattgggag gaacccgcg ggaaatgac gttgcaccc ttaaagagc aaccgaagg atatgtctg gttgggggt ctgaccacc gatacagcc cttcggacc gtcgcagag tcctttcgg gtgccggtt gttacttca aaagacgga gaagattgt gctcaagat gatagtttt agtaccgtt tttatatta gagagtttc gaaggagac gtatttaat tgtttatat aaacagaag cagtccctt atggggccc ttggctctg cagcagcta gccaacaag aatgagcca cttccagac aacacgagg cccctcttc aacctggct atgacgggg gtcgttgtc tgctttact ggattcagg aacaaaagt gatctggca actttggta ccactgatc catcatatg ggtggttcc atcaggaaa aacataagc cagaaagtg acgcattta attgccaac atttgtaat ggagaaaaa tatcgttat gcggccgta tttcgagta cctgtgatg aatcaagat tgggtctat gctagttgg aagaaaaga catgatatt aattttagt gcaacggtt gatgcattt gtggctgaa ttcaagttg aagcctttc catggtgct aaagtgtgt ttcgttggg tttgcagaa gatgagaaa aagcacatg actgatatt ttgttgtcc aatggcggg actgtgact agcttagag gatccccaa tgtagccat gtggtcgtg gatgagtcc tcggtggcc gaaagacct gaaacaacg tcacctaaa gttttccta gtgaaagct gaatggttc tggacctca gtacaaaag gagatatgt cttgacgag aaagactac ctctatgac gactatgtc gaacagacg ttttcgccg actcgaaga gaatcgcat caagcaacg ccaagtagc gccagtaga cggaaacgt aaacggttg cacgaagct gttttgtcg ctgacacat cagcagtca ccaggtttg cataagcga cgctcttca gtgagtgat gcaggactg ttgtcagtg tctggttct ttcctagac tgcaccgcc agtccagat aaagcttta accgatggc acggaaccg tccattgac acgcctagg aaagggctg actgccagg cagcaggtt ttcctggaa ttggtccaa acggagtcc aattatgtc gatatcctg cataccatc atgaccatg ttcaagaaa catttagaa gagatgccg gaggaggaa gctttgctc aacaacacc gaactgaac ttaattttt ggcaaagtg ccagcgata tacgagaca cacatgaaa atgcgcgac gagttgcga tgttttgca tcaacttgg tccgaagac cacacgata ggcagtatc attgtgaaa tattcgaaa gatctgctc aaggcctac ccaccttac attaattac ttcgaagaa atgaaggag gttttaacg aaatgtgat caaaacaag ccacgattt cacgcgttc ctgaaggcg tgtcagaca aaaccggaa tgtggcaga cagagtttg caggagttg atgatacgg cctgtgcaa cgactgggg agcatttgt ttgttgctt gcaaatctc ctgaaacat acgccgaaa tctaatccg gaccatgcc gaactccag cgagctctg gacggtttg aaggaaggc atggacctg ataaacgaa gacaagcga aaagctgaa gcccaagtg gcccttttc aacatgttt aacgacatt gataactgc ccaccggac attgtgtgc tcccaccgg aagttcata gcaaaggcg gaagtgatc caagtgtca acaactgaa gggctttcg agcaaaggc aactccttg gtcctcttc ctcttttcc gatttggta gaagtgtgt aaaaagaag tcaaagacg tttaataac gccaaaagc ccgaccggc agtgtcaac agtttgcaa aggccgaag ttgtacaaa cacgtcaag ttactgcca ttgaacacg atcaagaaa gtcatagat atcaaagaa accgagcag tgccagaaa gtgttctgt ttcgtttgt cggaataac gacgaattg aaagagaga gtgtattcg tttgctatg ccggacgag gatgccgat aaagcaggg ttcctgaaa accttgacg aaacaaatg gccaataac gcctgcaca cctgatgcg gataagttt ctggcttat ctcgagtcg catcagttg gatatagac actagtgat atgaacaaa ggaacgttg aataaggcg ttcaaattc gcgtcaagg acgaagttg aaggtggga cgggccttc tcgtttaac aaaacgccg tcgaagttg aaacgggcg gtttcttca atgatgtct ccatttggc agctcaaca gactaccta cacacgaca tag
- >TC010404 RA
tacctgacg aatcgaatc ggccaatca gagcgcttc atttgactt tgaagtttt ggtatggaa tggatggtc catttttag ttccaaaca aagtaaaac cgctcaaaa atgtgttta aggtagttt gtggtgtga taagtgtga gattttaac gttgtgata agagacggt tgtgggaca gacgcgaaa acgtgcgaa tttagtgct ttagtactg atggtgtaa ccaaaaagt cgcttcaaa atggacgca cgcattcag ccccaaagt tccatttgt agtgactca aattgggag gaacccgcg ggaaatgac gttgcaccc ttaaagagc aaccgaagg atatgtctg gttgggggt ctgaccacc gatacagcc cttcggacc gtcgcagag tcctttcgg gtgccggtt gttacttca aaagacgga gaagattgt gctcaagat gatagtttt agtaccgtt tttatatta gagagtttc gaaggagac gtatttaat tgtttatat aaacagaag cagtccctt atggggccc ttggctctg cagcagcta gccaacaag aatgagcca cttccagac aacacgagg cccctcttc aacctggct atgacgggg gtcgttgtc tgctttact ggattcagg aacaaaagt gatctggca actttggta ccactgatc catcatatg ggtggttcc atcaggaaa aacataagc cagaaagtg acgcattta attgccaac atttgtaat ggagaaaaa tatcgttat gcggccgta tttcgagta cctgtgatg aatcaagat tgggtctat gctagttgg aagaaaaga catgatatt aattttagt gcaacggtt gatgcattt gtggctgaa ttcaagttg aagcctttc catggtgct aaagtgtgt ttcgttggg tttgcagaa gatgagaaa aagcacatg actgatatt ttgttgtcc aatggcggg actgtgact agcttagag gatccccaa tgtagccat gtggtaatg gaacgttca gaagtctgc acacttgaa gccgctagt gcaccgctt tctcccact gaacccgtc aatccctca atccctgac cctcccgag catctaaat tacaacggt tttctcgaa acgttacac gaaaaatcc cgcgacagt tgcattgac gactcaaac ttgaacacc tccgagttt tcttcaggc agtcgtaag cggaaaatc ccgtcgacc gaatcttcc gtcgtgaaa acaaagcga agaaaagat aaatgtgcc agtgagcgg aaaagcaaa gtttcgggc attttcaaa acaccgctt aactattta tcgaaccgt cgacgtaca atcgacact ttcagtttt aacgaaacg ctaaatcag agtgtcgtt tccagctcg ggtgtgttt aatgttgat acggttgag aatttaagc gttttgaat cagagtcaa tttacgcca cagtcgagc aataaacag aaaaagaat ttgtttgcg cgcactttt agttcgtcc aagttcaat cgcagcaag ttgaagaaa tgtaatttg aatgcgacg aggctctcg tttagtgaa gcctccgat gttgatgga aatgaacct ctcaatgct agctgtttc ccgaatatt tcccttaat cctgtacca catagtgag cttcgcagt gattgttcc aaggacgca cgtggacct tctgtgcct actatatcg gtcgtggat gagtcctcg gtggccgaa agacctgaa acaacgtca cctaaagtt ttcctagtg aaagctgaa tggttctgg acctcagta caaaaggag atatgtctt gacgagaaa gactacctc tatgacgac tatgtcgaa cagacgttt tcgccgact cgaagagaa tcgcatcaa gcaacgcca agtagcgcc agtagacgg aaacgtaaa cggttgcac gaagctgtt ttgtcgctg acacatcag cagtcacca ggtttgcat aagcgacgc tcttcagtg agtgatgca ggactgttg tcagtgtct ggttctttc ctagactgc accgccagt ccagataaa gctttaacc gatggcacg gaaccgtcc attgacacg cctaggaaa gggctgact gccaggcag caggttttc ctggaattg gtccaaacg gagtccaat tatgtcgat atcctgcat accatcatg accatgttc aagaaacat ttagaagag atgccggag gaggaagct ttgctcaac aacaccgaa ctgaactta atttttggc aaagtgcca gcgatatac gagacacac atgaaaatg cgcgacgag ttgcgatgt tttgcatca acttggtcc gaagaccac acgataggc agtatcatt gtgaaatat tcgaaagat ctgctcaag gcctaccca ccttacatt aattacttc gaagaaatg aaggaggtt ttaacgaaa tgtgatcaa aacaagcca cgatttcac gcgttcctg aaggcgtgt cagacaaaa ccggaatgt ggcagacag agtttgcag gagttgatg atacggcct gtgcaacga ctggggagc atttgtttg ttgcttgca aatctcctg aaacatacg ccgaaatct aatccggac catgccgaa ctccagcga gctctggac ggtttgaag gaaggcatg gacctgata aacgaagac aagcgaaaa gctgaagcc caagtggcc cttttcaac atgtttaac gacattgat aactgccca ccggacatt gtgtgctcc caccggaag ttcatagca aaggcggaa gtgatccaa gtgtcaaca actgaaggg ctttcgagc aaaggcaac tccttggtc ctcttcctc ttttccgat ttggtagaa gtgtgtaaa aagaagtca aagacgttt aataacgcc aaaagcccg accggcagt gtcaacagt ttgcaaagg ccgaagttg tacaaacac gtcaagtta ctgccattg aacacgatc aagaaagtc atagatatc aaagaaacc gagcagtgc cagaaagtg ttctgtttc gtttgtcgg aataacgac gaattgaaa gagagagtg tattcgttt gctatgccg gacgaggat gccgataaa gcagggttc ctgaaaacc ttgacgaaa caaatggcc aataacgcc tgcacacct gatgcggat aagtttctg gcttatctc gagtcgcat cagttggat atagacact agtgatatg aacaaagga acgttgaat aaggcgttc aaattcgcg tcaaggacg aagttgaag gtgggacgg gccttctcg tttaacaaa acgccgtcg aagttgaaa cgggcggtt tcttcaatg atgtctcca tttggcagc tcaacagac tacctacac acgacatag aacaagaac aaattttgg cgaaatttt tgttggaaa ataatatgc agctggcgc taaaatggc ggcatcttt attcgtata gtatttatt gtaatttta atctttttt tctaaatat tttatgatt tgtaaaaac ctgatataa taaaatatt tagatgatt
- >TC010404 RB
tacctgacg aatcgaatc ggccaatca gagcgcttc atttgactt tgaagtttt ggtatggaa tggatggtc catttttag ttccaaaca aagtaaaac cgctcaaaa atgtgttta aggtagttt gtggtgtga taagtgtga gattttaac gttgtgata agagacggt tgtgggaca gacgcgaaa acgtgcgaa tttagtgct ttagtactg atggtgtaa ccaaaaagt cgcttcaaa atggacgca cgcattcag ccccaaagt tccatttgt agtgactca aattgggag gaacccgcg ggaaatgac gttgcaccc ttaaagagc aaccgaagg atatgtctg gttgggggt ctgaccacc gatacagcc cttcggacc gtcgcagag tcctttcgg gtgccggtt gttacttca aaagacgga gaagattgt gctcaagat gatagtttt agtaccgtt tttatatta gagagtttc gaaggagac gtatttaat tgtttatat aaacagaag cagtccctt atggggccc ttggctctg cagcagcta gccaacaag aatgagcca cttccagac aacacgagg cccctcttc aacctggct atgacgggg gtcgttgtc tgctttact ggattcagg aacaaaagt gatctggca actttggta ccactgatc catcatatg ggtggttcc atcaggaaa aacataagc cagaaagtg acgcattta attgccaac atttgtaat ggagaaaaa tatcgttat gcggccgta tttcgagta cctgtgatg aatcaagat tgggtctat gctagttgg aagaaaaga catgatatt aattttagt gcaacggtt gatgcattt gtggctgaa ttcaagttg aagcctttc catggtgct aaagtgtgt ttcgttggg tttgcagaa gatgagaaa aagcacatg actgatatt ttgttgtcc aatggcggg actgtgact agcttagag gatccccaa tgtagccat gtggtaatg gaacgttca gaagtctgc acacttgaa gccgctagt gcaccgctt tctcccact gaacccgtc aatccctca atccctgac cctcccgag catctaaat tacaacggt tttctcgaa acgttacac gaaaaatcc cgcgacagt tgcattgac gactcaaac ttgaacacc tccgagttt tcttcaggc agtcgtaag cggaaaatc ccgtcgacc gaatcttcc gtcgtgaaa acaaagcga agaaaagat aaatgtgcc agtgagcgg aaaagcaaa gtttcgggc attttcaaa acaccgctt aactattta tcgaaccgt cgacgtaca atcgacact ttcagtttt aacgaaacg ctaaatcag agtgtcgtt tccagctcg ggtgtgttt aatgttgat acggttgag aatttaagc gttttgaat cagagtcaa tttacgcca cagtcgagc aataaacag aaaaagaat ttgtttgcg cgcactttt agttcgtcc aagttcaat cgcagcaag ttgaagaaa tgtaatttg aatgcgacg aggctctcg tttagtgaa gcctccgat gttgatgga aatgaacct ctcaatgct agctgtttc ccgaatatt tcccttaat cctgtacca catagtgag cttcgcagt gattgttcc aaggacgca cgtggacct tctgtgcct actatatcg gtcgtggat gagtcctcg gtggccgaa agacctgaa acaacgtca cctaaagtt ttcctagtg aaagctgaa tggttctgg acctcagta caaaaggag atatgtctt gacgagaaa gactacctc tatgacgac tatgtcgaa cagacgttt tcgccgact cgaagagaa tcgcatcaa gcaacgcca agtagcgcc agtagacgg aaacgtaaa cggttgcac gaagctgtt ttgtcgctg acacatcag cagtcacca gactgcacc gccagtcca gataaagct ttaaccgat ggcacggaa ccgtccatt gacacgcct aggaaaggg ctgactgcc aggcagcag gttttcctg gaattggtc caaacggag tccaattat gtcgatatc ctgcatacc atcatgacc atgttcaag aaacattta gaagagatg ccggaggag gaagctttg ctcaacaac accgaactg aacttaatt tttggcaaa gtgccagcg atatacgag acacacatg aaaatgcgc gacgagttg cgatgtttt gcatcaact tggtccgaa gaccacacg ataggcagt atcattgtg aaatattcg aaagatctg ctcaaggcc tacccacct tacattaat tacttcgaa gaaatgaag gaggtttta acgaaatgt gatcaaaac aagccacga tttcacgcg ttcctgaag gcgtgtcag acaaaaccg gaatgtggc agacagagt ttgcaggag ttgatgata cggcctgtg caacgactg gggagcatt tgtttgttg cttgcaaat ctcctgaaa catacgccg aaatctaat ccggaccat gccgaactc cagcgagct ctggacggt ttgaaggaa ggcatggac ctgataaac gaagacaag cgaaaagct gaagcccaa gtggccctt ttcaacatg tttaacgac attgataac tgcccaccg gacattgtg tgctcccac cggaagttc atagcaaag gcggaagtg atccaagtg tcaacaact gaagggctt tcgagcaaa ggcaactcc ttggtcctc ttcctcttt tccgatttg gtagaagtg tgtaaaaag aagtcaaag acgtttaat aacgccaaa agcccgacc ggcagtgtc aacagtttg caaaggccg aagttgtac aaacacgtc aagttactg ccattgaac acgatcaag aaagtcata gatatcaaa gaaaccgag cagtgccag aaagtgttc tgtttcgtt tgtcggaat aacgacgaa ttgaaagag agagtgtat tcgtttgct atgccggac gaggatgcc gataaagca gggttcctg aaaaccttg acgaaacaa atggccaat aacgcctgc acacctgat gcggataag tttctggct tatctcgag tcgcatcag ttggatata gacactagt gatatgaac aaaggaacg ttgaataag gcgttcaaa ttcgcgtca aggacgaag ttgaaggtg ggacgggcc ttctcgttt aacaaaacg ccgtcgaag ttgaaacgg gcggtttct tcaatgatg tctccattt ggcagctca acagactac ctacacacg acatagaac aagaacaaa ttttggcga aatttttgt tggaaaata atatgcagc tggcgctaa aatggcggc atctttatt cgtatagta tttattgta attttaatc tttttttct aaatatttt atgatttgt aaaaacctg atataataa aatatttag atgatt
- >TC010404 RC
tacctgacg aatcgaatc ggccaatca gagcgcttc atttgactt tgaagtttt ggtatggaa tggatggtc catttttag ttccaaaca aagtaaaac cgctcaaaa atgtgttta aggtagttt gtggtgtga taagtgtga gattttaac gttgtgata agagacggt tgtgggaca gacgcgaaa acgtgcgaa tttagtgct ttagtactg atggtgtaa ccaaaaagt cgcttcaaa atggacgca cgcattcag ccccaaagt tccatttgt agtgactca aattgggag gaacccgcg ggaaatgac gttgcaccc ttaaagagc aaccgaagg atatgtctg gttgggggt ctgaccacc gatacagcc cttcggacc gtcgcagag tcctttcgg gtgccggtt gttacttca aaagacgga gaagattgt gctcaagat gatagtttt agtaccgtt tttatatta gagagtttc gaaggagac gtatttaat tgtttatat aaacagaag cagtccctt atggggccc ttggctctg cagcagcta gccaacaag aatgagcca cttccagac aacacgagg cccctcttc aacctggct atgacgggg gtcgttgtc tgctttact ggattcagg aacaaaagt gatctggca actttggta ccactgatc catcatatg ggtggttcc atcaggaaa aacataagc cagaaagtg acgcattta attgccaac atttgtaat ggagaaaaa tatcgttat gcggccgta tttcgagta cctgtgatg aatcaagat tgggtctat gctagttgg aagaaaaga catgatatt aattttagt gcaacggtt gatgcattt gtggctgaa ttcaagttg aagcctttc catggtgct aaagtgtgt ttcgttggg tttgcagaa gatgagaaa aagcacatg actgatatt ttgttgtcc aatggcggg actgtgact agcttagag gatccccaa tgtagccat gtggtcgtg gatgagtcc tcggtggcc gaaagacct gaaacaacg tcacctaaa gttttccta gtgaaagct gaatggttc tggacctca gtacaaaag gagatatgt cttgacgag aaagactac ctctatgac gactatgtc gaacagacg ttttcgccg actcgaaga gaatcgcat caagcaacg ccaagtagc gccagtaga cggaaacgt aaacggttg cacgaagct gttttgtcg ctgacacat cagcagtca ccaggtttg cataagcga cgctcttca gtgagtgat gcaggactg ttgtcagtg tctggttct ttcctagac tgcaccgcc agtccagat aaagcttta accgatggc acggaaccg tccattgac acgcctagg aaagggctg actgccagg cagcaggtt ttcctggaa ttggtccaa acggagtcc aattatgtc gatatcctg cataccatc atgaccatg ttcaagaaa catttagaa gagatgccg gaggaggaa gctttgctc aacaacacc gaactgaac ttaattttt ggcaaagtg ccagcgata tacgagaca cacatgaaa atgcgcgac gagttgcga tgttttgca tcaacttgg tccgaagac cacacgata ggcagtatc attgtgaaa tattcgaaa gatctgctc aaggcctac ccaccttac attaattac ttcgaagaa atgaaggag gttttaacg aaatgtgat caaaacaag ccacgattt cacgcgttc ctgaaggcg tgtcagaca aaaccggaa tgtggcaga cagagtttg caggagttg atgatacgg cctgtgcaa cgactgggg agcatttgt ttgttgctt gcaaatctc ctgaaacat acgccgaaa tctaatccg gaccatgcc gaactccag cgagctctg gacggtttg aaggaaggc atggacctg ataaacgaa gacaagcga aaagctgaa gcccaagtg gcccttttc aacatgttt aacgacatt gataactgc ccaccggac attgtgtgc tcccaccgg aagttcata gcaaaggcg gaagtgatc caagtgtca acaactgaa gggctttcg agcaaaggc aactccttg gtcctcttc ctcttttcc gatttggta gaagtgtgt aaaaagaag tcaaagacg tttaataac gccaaaagc ccgaccggc agtgtcaac agtttgcaa aggccgaag ttgtacaaa cacgtcaag ttactgcca ttgaacacg atcaagaaa gtcatagat atcaaagaa accgagcag tgccagaaa gtgttctgt ttcgtttgt cggaataac gacgaattg aaagagaga gtgtattcg tttgctatg ccggacgag gatgccgat aaagcaggg ttcctgaaa accttgacg aaacaaatg gccaataac gcctgcaca cctgatgcg gataagttt ctggcttat ctcgagtcg catcagttg gatatagac actagtgat atgaacaaa ggaacgttg aataaggcg ttcaaattc gcgtcaagg acgaagttg aaggtggga cgggccttc tcgtttaac aaaacgccg tcgaagttg aaacgggcg gtttcttca atgatgtct ccatttggc agctcaaca gactaccta cacacgaca tagaacaag aacaaattt tggcgaaat ttttgttgg aaaataata tgcagctgg cgctaaaat ggcggcatc tttattcgt atagtattt attgtaatt ttaatcttt ttttctaaa tattttatg atttgtaaa aacctgata taataaaat atttagatg att
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