Status
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Gene information
General information
TC010370 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24561245..24570895
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010370
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04824

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010370 RA
        MTEHEHCKI FRPDPVPTY ASICAAMSS TSLATPSCP 
        STVSPDRSS LKVNCDKNS KKVHFERTR EPKSRRITQ 
        KKEPLKLPR IKKRYFTAG TGINDRNQR DLLNEPSPF 
        VYPKDHEEF AHKVMTKEW RSDLRRKEM GYKYNERHN 
        YKSRQQGDM LNRLYVKEP EIRSAKMVQ DVDPEFFTI 
        VQGRPIPEK LKLSRYIQT VRDVLKTKI LTGYRGDDI 
        MLIDESLIL EQKEIDRIK DNYQTYVNT FEEFLYNDH 
        TSSMNLLKE SDREAMLAQ EKYEEYRQL SREYGALKS 
        VLYSTEEKW RNCKLYQRF LYLVSPISW RKEHDYYYM 
        QEGDLAAFQ EVSSIFGKY RLNVTDETA SLEDLINQF 
        REDCASQKE PALYFTDPN QLLDVFRFM ELQNLNSLL 
        HSEELAVPL ENVQEGMAR AEELFNAEI NSLQELIEK 
        LASGISWEE ERAKYLEEL ALELINGEF KKLVIAEEV 
        LNLHVFVED VYETRIGPN DANLSMSEM MKMIESKFR 
        EELISLDQM PSEQVAQLE VSCYREEAK MMKAAQQAA 
        RQLLEFEML FLKLQKVFS PPHKRTTRE LKWRSPPVN 
        PPKPVPPPP RLLTEEEKE FLEFFTEYC RYTDDLADY 
        GIDISHPQE PEAEKTSAN KERSRLLLQ E
      CDS sequence
      • >TC010370 RA
        atgacggaa catgaacat tgtaaaata tttcgcccc 
        gacccagtg cccacctat gcctcaata tgtgcagcc 
        atgtcttcc acctccctc gccacccct tcatgcccc 
        agcaccgtc tccccagac cgctcatcc ctcaaagta 
        aactgcgac aaaaactcg aaaaaagtg cactttgag 
        cggacaaga gagccgaaa tcgcgccga atcacccaa 
        aagaaggag cctctgaaa ctgccaagg atcaagaaa 
        cggtatttc actgcagga actgggata aatgaccgg 
        aatcagcgg gatttgctc aatgagccg tccccgttc 
        gtctacccg aaggaccac gaggagttc gcccacaaa 
        gtcatgacc aaggagtgg aggtccgac ttgaggcgc 
        aaggaaatg ggttacaag tacaacgaa cggcacaat 
        tacaagtcg aggcaacaa ggcgatatg ctgaaccgc 
        ttgtacgtt aaagagcct gaaattagg agcgcgaag 
        atggtgcaa gacgtggat cctgaattt ttcacgatt 
        gtgcaaggg agaccgatc cctgaaaaa ctgaaattg 
        agtcgttac atacagacg gtgcgagat gtgctgaag 
        actaaaatc ctgactggg tacagaggt gacgatatt 
        atgctgatt gatgagagc ttgatcctg gagcagaag 
        gaaattgac cggattaaa gacaattat cagacttat 
        gtcaacacc ttcgaggag ttcctgtac aatgatcac 
        acctcctcg atgaacttg ctgaaagag tcagatcgg 
        gaagcaatg ttagcccag gaaaaatac gaggaatat 
        cgccagtta tctcgagag tatggcgct ttgaagtca 
        gttctgtac agcactgag gaaaagtgg cgtaactgt 
        aagttgtac cagaggttt ctctacctg gtcagcccc 
        atatcctgg aggaaggag catgattac tactacatg 
        caagaggga gatcttgct gcgtttcag gaagtgtcg 
        tccattttc ggtaaatac cgcctgaat gtgactgac 
        gaaactgcc tcgttggaa gatttaatc aaccagttt 
        cgggaagac tgcgcgagt cagaaagag ccagcgctg 
        tattttact gacccgaat caactactc gacgttttc 
        agatttatg gaacttcaa aacttgaac tccctcctg 
        cattccgag gagttggcc gttcctttg gaaaatgtg 
        caagaggga atggctcgg gcggaggaa ttattcaat 
        gctgagatc aactcgctg caggaactt atcgagaaa 
        ttagcgagt gggatttcg tgggaggag gaacgtgcc 
        aagtacttg gaggaatta gctttggaa ctaatcaat 
        ggggaattc aagaaattg gtaatcgct gaggaagtt 
        ctgaatctg cacgtgttc gtcgaagac gtttatgaa 
        actcggatc ggtccgaac gatgccaat ttgagcatg 
        agcgagatg atgaaaatg attgaatcg aaatttcgc 
        gaggagctt atcagtctc gatcaaatg ccttccgag 
        caagtggcg caactggaa gttagctgc tatagggaa 
        gaggcgaaa atgatgaaa gcagcgcaa caagccgca 
        aggcagtta cttgaattt gaaatgctc ttcctgaag 
        ttgcagaag gtgttttcg ccgccgcat aagaggacc 
        actagagag ctgaaatgg agatctcct cctgtgaat 
        ccaccaaag ccggtgccg ccccctccg agactgctc 
        accgaagag gagaaagag tttcttgaa tttttcacc 
        gaatattgc agatatacc gatgatctg gcggattat 
        gggattgat atctcgcat ccgcaagaa ccagaggct 
        gaaaagacc tcggcgaat aaagaacgt tcacgactg 
        ttattacag gaataa
      mRNA sequence
      • >TC010370 RA
        cacgtattg acaaaactt ctcataatg acggaacat 
        gaacattgt aaaatattt cgccccgac ccagtgccc 
        acctatgcc tcaatatgt gcagccatg tcttccacc 
        tccctcgcc accccttca tgccccagc accgtctcc 
        ccagaccgc tcatccctc aaagtaaac tgcgacaaa 
        aactcgaaa aaagtgcac tttgagcgg acaagagag 
        ccgaaatcg cgccgaatc acccaaaag aaggagcct 
        ctgaaactg ccaaggatc aagaaacgg tatttcact 
        gcaggaact gggataaat gaccggaat cagcgggat 
        ttgctcaat gagccgtcc ccgttcgtc tacccgaag 
        gaccacgag gagttcgcc cacaaagtc atgaccaag 
        gagtggagg tccgacttg aggcgcaag gaaatgggt 
        tacaagtac aacgaacgg cacaattac aagtcgagg 
        caacaaggc gatatgctg aaccgcttg tacgttaaa 
        gagcctgaa attaggagc gcgaagatg gtgcaagac 
        gtggatcct gaatttttc acgattgtg caagggaga 
        ccgatccct gaaaaactg aaattgagt cgttacata 
        cagacggtg cgagatgtg ctgaagact aaaatcctg 
        actgggtac agaggtgac gatattatg ctgattgat 
        gagagcttg atcctggag cagaaggaa attgaccgg 
        attaaagac aattatcag acttatgtc aacaccttc 
        gaggagttc ctgtacaat gatcacacc tcctcgatg 
        aacttgctg aaagagtca gatcgggaa gcaatgtta 
        gcccaggaa aaatacgag gaatatcgc cagttatct 
        cgagagtat ggcgctttg aagtcagtt ctgtacagc 
        actgaggaa aagtggcgt aactgtaag ttgtaccag 
        aggtttctc tacctggtc agccccata tcctggagg 
        aaggagcat gattactac tacatgcaa gagggagat 
        cttgctgcg tttcaggaa gtgtcgtcc attttcggt 
        aaataccgc ctgaatgtg actgacgaa actgcctcg 
        ttggaagat ttaatcaac cagtttcgg gaagactgc 
        gcgagtcag aaagagcca gcgctgtat tttactgac 
        ccgaatcaa ctactcgac gttttcaga tttatggaa 
        cttcaaaac ttgaactcc ctcctgcat tccgaggag 
        ttggccgtt cctttggaa aatgtgcaa gagggaatg 
        gctcgggcg gaggaatta ttcaatgct gagatcaac 
        tcgctgcag gaacttatc gagaaatta gcgagtggg 
        atttcgtgg gaggaggaa cgtgccaag tacttggag 
        gaattagct ttggaacta atcaatggg gaattcaag 
        aaattggta atcgctgag gaagttctg aatctgcac 
        gtgttcgtc gaagacgtt tatgaaact cggatcggt 
        ccgaacgat gccaatttg agcatgagc gagatgatg 
        aaaatgatt gaatcgaaa tttcgcgag gagcttatc 
        agtctcgat caaatgcct tccgagcaa gtggcgcaa 
        ctggaagtt agctgctat agggaagag gcgaaaatg 
        atgaaagca gcgcaacaa gccgcaagg cagttactt 
        gaatttgaa atgctcttc ctgaagttg cagaaggtg 
        ttttcgccg ccgcataag aggaccact agagagctg 
        aaatggaga tctcctcct gtgaatcca ccaaagccg 
        gtgccgccc cctccgaga ctgctcacc gaagaggag 
        aaagagttt cttgaattt ttcaccgaa tattgcaga 
        tataccgat gatctggcg gattatggg attgatatc 
        tcgcatccg caagaacca gaggctgaa aagacctcg 
        gcgaataaa gaacgttca cgactgtta ttacaggaa 
        taaaaatct ataagcctg tgttacttt cgttttatt 
        tttcattcc actgattaa agctcacac cttcaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY