Status
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Gene information
General information
TC010368 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24506365..24511102
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010368
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04823

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010368 RA
      MPSNGPGYA TPLDAMKNG PREELLYVV CIQPNQAEG 
      KTDLLATVD VNPSSPTYC QVIHRLRTG RLNDELHHS 
      GWNICSSCH DTKGCCDVP KRDKLILPA LNSDRIYVV 
      DTGKNPRAP TMHKVIEAE EMHRFNCTT PHTAHCLAS 
      GEVMISTMG DNQGNGKCE FILLDGKTF EVKGTWTRN 
      KSGAFNYDF WYQPYFDIM VSSEWGAPK IFKRGFQPT 
      DPDDQKIYG RSLNFFSWS KRELLQTVN LGFDGVTPL 
      EIRFLHNPK QPQGFVGCA LNANIFRFY QKSDGTWAV 
      DKVIDIPAK KVSGWDGDY INGMITDIL LSLDDKYLY 
      LNNWLHGDV RQYDISDPA HPKLTGQLF LGGKILKDS 
      KIKVLEDKE LKEQPDPVY IKGRRILGG PQMMQLSLD 
      GKRLYISSS LFSPWDRQF YPETIEKGA TIVKIDIDT 
      QRGGMKLDE NFLVDFGVG PDGPLLAHE MRYPGGDCT 
      SDIWLAQE
    CDS sequence
    • >TC010368 RA
      atgccttca aacggacct ggatacgct actcctctc 
      gatgccatg aaaaatggc cctcgtgaa gaactgctc 
      tacgttgtt tgtattcaa cccaaccaa gctgaagga 
      aaaaccgac ctgttagca actgtggac gttaaccca 
      tcgtcacct acttattgc caagttatt cacagattg 
      aggaccgga agacttaac gatgaactg caccacagc 
      ggatggaat atctgttcc agttgtcat gacacgaag 
      ggctgctgt gatgttccc aaaagggat aaactcatt 
      ttgccggcg ttgaactcc gaccgaatt tatgtcgtc 
      gatacgggg aaaaatcca agggctcca actatgcac 
      aaagtgatc gaagctgaa gaaatgcac cgctttaac 
      tgcaccacc ccccacact gcccactgc ctggcaagt 
      ggtgaagtg atgatttcg acaatgggt gacaatcaa 
      gggaacggc aaatgcgag tttatttta ctcgatggt 
      aaaaccttc gaagtgaag ggaacctgg accaggaac 
      aagagcgga gccttcaat tatgacttt tggtaccag 
      ccctacttt gacataatg gtctcatca gagtggggg 
      gctcccaaa attttcaaa cgtgggttc caacccact 
      gaccccgac gaccaaaaa atctacgga cgctccctc 
      aattttttc tcttggtct aaacgagag ctcctccaa 
      acggttaat ttggggttt gatggagtg acccctctt 
      gaaattcgg tttttgcac aacccgaaa cagccccaa 
      gggttcgtt ggctgcgcc ctcaacgct aatatattc 
      aggttttat caaaaaagt gatggaact tgggctgtt 
      gacaaggtc attgatatt ccagctaag aaagtttcg 
      ggctgggat ggagattac atcaacggg atgataaca 
      gatattttg ttatcactc gacgacaaa tacctttat 
      ctcaacaat tggctacat ggcgatgtc agacagtac 
      gatatttcg gatcccgcg catccgaaa ttgacgggg 
      caattattt ttgggtgga aaaattttg aaagattct 
      aagattaaa gtcttggaa gataaagaa ttgaaggaa 
      cagccggat cctgtctac attaaggga aggcgcatc 
      ttgggtggt ccccaaatg atgcaattg tctcttgat 
      ggaaagcgg ctgtacatt agctcgagt ttgttctcg 
      ccttgggac cgacagttt tatcccgag acaatagaa 
      aaaggtgca actattgtt aaaatcgat attgataca 
      cagagaggt gggatgaag ttggatgag aactttttg 
      gtcgatttt ggagtcggg cctgatgga ccattatta 
      gctcatgaa atgagatac cctggaggt gattgcaca 
      tcagacatt tggcttgca caagagtaa
    mRNA sequence
    • >TC010368 RA
      caactgaaa caactaaaa ccagaaaca agcacacaa 
      acatatctt atccattta tgagttatt tgtccactt 
      gttcataag cgattgatt tggaaatcg tctgtctct 
      caactcagt attcaccat gccttcaaa cggacctgg 
      atacgctac tcctctcga tgccatgaa aaatggccc 
      tcgtgaaga actgctcta cgttgtttg tattcaacc 
      caaccaagc tgaaggaaa aaccgacct gttagcaac 
      tgtggacgt taacccatc gtcacctac ttattgcca 
      agttattca cagattgag gaccggaag acttaacga 
      tgaactgca ccacagcgg atggaatat ctgttccag 
      ttgtcatga cacgaaggg ctgctgtga tgttcccaa 
      aagggataa actcatttt gccggcgtt gaactccga 
      ccgaattta tgtcgtcga tacggggaa aaatccaag 
      ggctccaac tatgcacaa agtgatcga agctgaaga 
      aatgcaccg ctttaactg caccacccc ccacactgc 
      ccactgcct ggcaagtgg tgaagtgat gatttcgac 
      aatgggtga caatcaagg gaacggcaa atgcgagtt 
      tattttact cgatggtaa aaccttcga agtgaaggg 
      aacctggac caggaacaa gagcggagc cttcaatta 
      tgacttttg gtaccagcc ctactttga cataatggt 
      ctcatcaga gtggggggc tcccaaaat tttcaaacg 
      tgggttcca acccactga ccccgacga ccaaaaaat 
      ctacggacg ctccctcaa ttttttctc ttggtctaa 
      acgagagct cctccaaac ggttaattt ggggtttga 
      tggagtgac ccctcttga aattcggtt tttgcacaa 
      cccgaaaca gccccaagg gttcgttgg ctgcgccct 
      caacgctaa tatattcag gttttatca aaaaagtga 
      tggaacttg ggctgttga caaggtcat tgatattcc 
      agctaagaa agtttcggg ctgggatgg agattacat 
      caacgggat gataacaga tattttgtt atcactcga 
      cgacaaata cctttatct caacaattg gctacatgg 
      cgatgtcag acagtacga tatttcgga tcccgcgca 
      tccgaaatt gacggggca attattttt gggtggaaa 
      aattttgaa agattctaa gattaaagt cttggaaga 
      taaagaatt gaaggaaca gccggatcc tgtctacat 
      taagggaag gcgcatctt gggtggtcc ccaaatgat 
      gcaattgtc tcttgatgg aaagcggct gtacattag 
      ctcgagttt gttctcgcc ttgggaccg acagtttta 
      tcccgagac aatagaaaa aggtgcaac tattgttaa 
      aatcgatat tgatacaca gagaggtgg gatgaagtt 
      ggatgagaa ctttttggt cgattttgg agtcgggcc 
      tgatggacc attattagc tcatgaaat gagataccc 
      tggaggtga ttgcacatc agacatttg gcttgcaca 
      agagtaaaa aaaaaagat gagataaga aaacgcttc 
      ataattgct tagtaataa ataaatatt cttgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY