Status
Please wait ...
Gene information
General information
TC032583
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010357,TC010356,TC010358
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24093417..24101996
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032583
    iBeetle screen iB_04819

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032583 RA
      TPKNPLELF HTYTDSQNY VNAENSETE INGHILCIA 
      IEQVEIITR KIKTNWHYL QSVVLNETT IASDDLIKL 
      REEINKCLK LIIECLKLC ESDILKERY VTSSWLHIK 
      KIAQLTLPI SELERDIYL KLVSPSNRD RCPVFQYFH 
      TFLDVQFYL LMFDYVCGN SEELQDRIN IVVNDLVTL 
      SASGHKQKS NSAFVCNCT KNLWLIIQK LCEKHLEGS 
      FWPIFNKSV ENLNGDCIL WLLKEVATL QASNNFKQP 
      NFTLLEPSL KKVLSGADF NTSILPILK SLEPLLCDL 
      WLKSSKIEI YQALWDHFS KRLNISFRG STDFTLTQL 
      VDLIDTILW SGKDCEYDF ELYLAMLTT HLSEHPYHW 
      GKMKGRIYS QLGPNKLKD LQKTGIFNV LLLYLALTK 
      VNFEELSKK FLSFIENLP AEKRQTQLI WSFYAAFMV 
      RHVRENRDI ETITQPFIK MSEEASTDQ KFSHLLKTF 
      VSDFDNIIT ISANLQLHQ WLLINTWIH QYMTTCYYP 
      DLLTLLTTL LKIIDRIST ELMWCEWES AFKNNVYPT 
      LKYVAGSRS APETCGTLA AKIATLTPT LQSEAFTFF 
      NAENIAPKV SANFLCTIL ENYPTCCLT PAQEIVALQ 
      TWVKFCLLT RDGYEELTN QIVKLGVIP PFIKNAIIK 
      VKDPLCAFI EYIGSNIKE YAQSVTMSK LCENFFGQT 
      ERWIGQFLA QPDNEATVL RIFTCIGLA FYKLGPLLY 
      NKHKSSCPY ARLVSAVLL PTELLIGKP IHAFVLQSV 
      KKTWHLFFE ATVKLNSND AFLDRILRD MVVKYVPHF 
      AIADSPMLK CLDNETNAE IILEKLSNA FFKQPSKEA 
      ESTISKSLK LVNDFVQST TSEKSLKNM ALDAIKSIT 
      CSPLYAKVR AEFEDIVVA VTEKHLAFN SPNYFQLMG 
      HLARMAPET VKNITPRVK QHLANVERM RGVGNDAFL 
      DRILRDMVV KYVPHFAIA DSPMLKCLD NETNVEIIL 
      EKLSNAFFK QPTKEAEST ISKSLKLVN DFVQSTTSE 
      KSLKLVVDK TLYGLLEVV MFHNRRNMA LDAIKSITC 
      SSLYAKVKA EFEDIVVAV TEKHLAFNS PNYFQLMGH 
      LARIAPETV KNITPRVKQ HLANVERMR GVGNDAFLD 
      RILRDMVVK YVPHFAIAD SPMLKCLDN ETNVEIILE 
      KLSNAFFKQ PTKEAESTI SKSLKLVND FVQSTTSEK 
      SLKLVVDKT LYGLLEVVM FHNRRNMAL DAIKSITCS 
      PLYAKVRAE FEDTVVAVT EKHLAFNSP NYFQLMGHL 
      ARIAPETVK NITPRVKQH LANVERMRG VGYDKNLRL 
      NLERLEASL LHKS
    CDS sequence
    • >TC032583 RA
      acgccgaaa aacccccta gaattgttc cacacttac 
      acagattct caaaattat gtgaacgct gaaaattcg 
      gagactgaa atcaacggc cacatttta tgcattgcg 
      attgaacaa gtcgaaatt atcactaga aaaatcaaa 
      accaactgg cattacctc caaagtgtg gttttaaac 
      gaaacaaca atagcctca gacgaccta atcaaatta 
      cgagaagag atcaacaag tgtttgaaa ctaattatc 
      gagtgtttg aagttgtgt gagagtgat atcctcaag 
      gagcgctat gtcacgtct tcgtggctt cacatcaag 
      aaaattgca caattaact ttgccaata tcggagtta 
      gaacgggat atttacttg aagttagta agtccaagt 
      aatagggac aggtgcccc gtttttcaa tacttccac 
      acttttttg gacgtccag ttttactta ctaatgttt 
      gattatgtt tgcggtaac agtgaggag ctgcaagac 
      cgcataaat atcgtcgtt aatgattta gtgacgttg 
      agtgcaagt ggacacaaa cagaaaagt aactctgcg 
      tttgtttgc aactgcacg aaaaacttg tggttgatt 
      attcaaaaa ttatgtgag aaacacttg gaagggtct 
      ttctggccg atatttaat aaaagtgtg gagaatctc 
      aacggtgat tgtattttg tggctgctt aaagaggta 
      gcaactcta caagcttca aataatttt aaacaacca 
      aatttcaca ctactagaa ccatcactg aaaaaagtc 
      ctgtcagga gcagacttc aacacttcc attttacca 
      attttgaag tctttagag cctcttttg tgtgatttg 
      tggttaaag tcgtcaaaa atcgaaata tatcaagcg 
      ctttgggac cattttagt aagagattg aacatatcg 
      ttcagaggc agtacggat ttcactcta actcagtta 
      gttgattta attgatacg atactatgg tccgggaag 
      gattgtgaa tacgatttt gaactgtac ttggctatg 
      ttgacaacg catttgagc gaacacccg taccattgg 
      ggcaaaatg aaagggcgg atttattcg cagttgggt 
      ccaaataaa ctaaaagat ttgcaaaaa accggaatt 
      ttcaacgtc ttgctgctg tatctcgca ttgaccaaa 
      gtcaacttt gaagagctg tccaagaag tttctttcg 
      tttattgag aatttgccg gcggagaaa aggcaaact 
      caactgatt tggagcttt tatgcggca tttatggtt 
      cgccacgtg cgcgaaaac cgagacatc gaaaccatc 
      acccaacca tttatcaaa atgtccgaa gaagcatca 
      accgaccaa aaattctcc catttgctc aaaacgttc 
      gtctcagac ttcgacaac atcatcacc atcagcgca 
      aacctccag ctccaccaa tggctcctc atcaacacc 
      tggatccac caatacatg accacctgc tactacccc 
      gacctctta accctcctt accaccctc ctgaaaata 
      atcgatcga atcagcacc gaactaatg tggtgcgag 
      tgggaatcc gcattcaaa aacaacgtc taccccact 
      ttgaaatac gtggcgggg tcgcgcagc gcccccgaa 
      acttgcggc accctagct gcgaaaatc gcaacactg 
      actccaacc ctacaatcg gaagccttc accttcttc 
      aacgccgaa aacatcgca ccgaaagtc tcggcaaat 
      tttctctgc acaatcctc gaaaactac cccacgtgt 
      tgtttaaca cccgcccag gaaatcgta gctttgcaa 
      acttgggtc aaattctgc cttctgacc cgagacggt 
      tacgaggaa ctcaccaat cagattgtt aagctgggg 
      gtgattccc ccgtttatc aaaaacgcg atcataaaa 
      gtcaaagac ccgctgtgt gccttcatc gaatacatc 
      ggcagtaat ataaaagaa tacgctcag tcggtcaca 
      atgagcaaa ctgtgcgag aattttttc ggccagact 
      gagcgctgg attggccag tttttggcg cagcctgat 
      aacgaagcc accgttttg cgaatattc acatgcata 
      gggctcgct ttttacaaa ctggggccc ttattgtac 
      aacaaacac aagtcgagt tgtccatac gcccgtctt 
      gtctctgcc gttttgtta ccaactgag ctcctaatc 
      gggaaaccc atccatgcg ttcgtttta caaagtgtg 
      aaaaaaacc tggcattta tttttcgaa gccactgtt 
      aaattaaac agcaatgat gcgtttctc gaccgaatt 
      ttgcgcgat atggttgtg aaatacgtg ccgcatttt 
      gcaattgcc gactcgcca atgctcaaa tgcctcgac 
      aatgaaacc aacgcggaa ataatccta gagaaatta 
      tcaaacgcc ttcttcaaa caaccgtca aaagaggcc 
      gaatcaaca atttccaaa tcgctgaaa ttagttaac 
      gattttgtc caaagcact acgtccgaa aagtcactc 
      aaaaatatg gcactggat gcgattaaa agcataacg 
      tgttcgccg ttgtacgca aaagttagg gccgagttt 
      gaggatatt gtagtggca gtgactgaa aaacatttg 
      gcgtttaat agtccgaat tattttcag ttgatggga 
      catttggca cgaatggct cccgagact gtgaaaaat 
      ataactcct agagttaag caacatttg gcgaatgtg 
      gagaggatg cgaggggtt ggcaatgat gcgtttctc 
      gaccgaatt ttgcgcgat atggttgtg aaatacgtg 
      ccgcatttt gcaattgcc gactcgcca atgctcaaa 
      tgcctcgac aatgaaacc aacgtggaa ataatccta 
      gagaaatta tcaaacgcc ttcttcaaa caaccgaca 
      aaagaagcc gaatcaaca atttccaaa tcgctgaaa 
      ttagttaac gattttgtc caaagcact acgtccgaa 
      aagtcacta aagttggtt gtagataaa acattgtat 
      ggcctcctt gaggtcgtg atgtttcac aatcgcaga 
      aatatggca ctggatgcg attaaaagc ataacgtgt 
      tcgtcgttg tacgcaaaa gttaaggcc gagtttgag 
      gatattgta gtggcagtg actgaaaaa catttggcg 
      tttaatagt ccgaattat tttcagttg atggggcat 
      ttggcacga atagctccc gagactgtg aaaaatata 
      actcctaga gttaagcaa catttggcg aatgtggag 
      aggatgcga ggggttggc aatgatgcg tttctcgac 
      cgaattttg cgcgatatg gttgtgaaa tacgtgccg 
      cattttgca attgccgac tcgccaatg ctcaaatgc 
      ctcgacaat gaaaccaac gtggaaata atcctagag 
      aaattatca aacgccttc ttcaaacaa ccgacaaaa 
      gaagccgaa tcaacaatt tccaaatcg ctgaaatta 
      gttaacgat tttgtccaa agcactacg tccgaaaag 
      tcactaaag ttggttgta gataaaaca ctgtatggc 
      ctccttgag gtcgtgatg tttcacaat cgcagaaat 
      atggcactg gatgcgatt aaaagcata acgtgttcg 
      ccgttgtac gcgaaagtt cgggccgag tttgaggat 
      actgtagtg gcagtgact gaaaaacat ttggcgttt 
      aatagtccg aattatttt cagttgatg gggcatttg 
      gcacgaata gctcccgag actgtgaaa aatataact 
      cctagagtt aagcaacat ttggcgaat gtggagagg 
      atgcgaggg gttgggtat gataaaaat ttgcggctg 
      aatttggaa agactggaa gcttcgctg ttacataaa 
      tcttaa
    mRNA sequence
    • >TC032583 RA
      aaacgccga aaaaccccc tagaattgt tccacactt 
      acacagatt ctcaaaatt atgtgaacg ctgaaaatt 
      cggagactg aaatcaacg gccacattt tatgcattg 
      cgattgaac aagtcgaaa ttatcacta gaaaaatca 
      aaaccaact ggcattacc tccaaagtg tggttttaa 
      acgaaacaa caatagcct cagacgacc taatcaaat 
      tacgagaag agatcaaca agtgtttga aactaatta 
      tcgagtgtt tgaagttgt gtgagagtg atatcctca 
      aggagcgct atgtcacgt cttcgtggc ttcacatca 
      agaaaattg cacaattaa ctttgccaa tatcggagt 
      tagaacggg atatttact tgaagttag taagtccaa 
      gtaataggg acaggtgcc ccgtttttc aatacttcc 
      acacttttt tggacgtcc agttttact tactaatgt 
      ttgattatg tttgcggta acagtgagg agctgcaag 
      accgcataa atatcgtcg ttaatgatt tagtgacgt 
      tgagtgcaa gtggacaca aacagaaaa gtaactctg 
      cgtttgttt gcaactgca cgaaaaact tgtggttga 
      ttattcaaa aattatgtg agaaacact tggaagggt 
      ctttctggc cgatattta ataaaagtg tggagaatc 
      tcaacggtg attgtattt tgtggctgc ttaaagagg 
      tagcaactc tacaagctt caaataatt ttaaacaac 
      caaatttca cactactag aaccatcac tgaaaaaag 
      tcctgtcag gagcagact tcaacactt ccattttac 
      caattttga agtctttag agcctcttt tgtgtgatt 
      tgtggttaa agtcgtcaa aaatcgaaa tatatcaag 
      cgctttggg accatttta gtaagagat tgaacatat 
      cgttcagag gcagtacgg atttcactc taactcagt 
      tagttgatt taattgata cgatactat ggtccggga 
      aggattgtg aatacgatt ttgaactgt acttggcta 
      tgttgacaa cgcatttga gcgaacacc cgtaccatt 
      ggggcaaaa tgaaagggc ggatttatt cgcagttgg 
      gtccaaata aactaaaag atttgcaaa aaaccggaa 
      ttttcaacg tcttgctgc tgtatctcg cattgacca 
      aagtcaact ttgaagagc tgtccaaga agtttcttt 
      cgtttattg agaatttgc cggcggaga aaaggcaaa 
      ctcaactga tttggagct tttatgcgg catttatgg 
      ttcgccacg tgcgcgaaa accgagaca tcgaaacca 
      tcacccaac catttatca aaatgtccg aagaagcat 
      caaccgacc aaaaattct cccatttgc tcaaaacgt 
      tcgtctcag acttcgaca acatcatca ccatcagcg 
      caaacctcc agctccacc aatggctcc tcatcaaca 
      cctggatcc accaataca tgaccacct gctactacc 
      ccgacctct taaccctcc ttaccaccc tcctgaaaa 
      taatcgatc gaatcagca ccgaactaa tgtggtgcg 
      agtgggaat ccgcattca aaaacaacg tctacccca 
      ctttgaaat acgtggcgg ggtcgcgca gcgcccccg 
      aaacttgcg gcaccctag ctgcgaaaa tcgcaacac 
      tgactccaa ccctacaat cggaagcct tcaccttct 
      tcaacgccg aaaacatcg caccgaaag tctcggcaa 
      attttctct gcacaatcc tcgaaaact accccacgt 
      gttgtttaa cacccgccc aggaaatcg tagctttgc 
      aaacttggg tcaaattct gccttctga cccgagacg 
      gttacgagg aactcacca atcagattg ttaagctgg 
      gggtgattc ccccgttta tcaaaaacg cgatcataa 
      aagtcaaag acccgctgt gtgccttca tcgaataca 
      tcggcagta atataaaag aatacgctc agtcggtca 
      caatgagca aactgtgcg agaattttt tcggccaga 
      ctgagcgct ggattggcc agtttttgg cgcagcctg 
      ataacgaag ccaccgttt tgcgaatat tcacatgca 
      tagggctcg ctttttaca aactggggc ccttattgt 
      acaacaaac acaagtcga gttgtccat acgcccgtc 
      ttgtctctg ccgttttgt taccaactg agctcctaa 
      tcgggaaac ccatccatg cgttcgttt tacaaagtg 
      tgaaaaaaa cctggcatt tatttttcg aagccactg 
      ttaaattaa acagcaatg atgcgtttc tcgaccgaa 
      ttttgcgcg atatggttg tgaaatacg tgccgcatt 
      ttgcaattg ccgactcgc caatgctca aatgcctcg 
      acaatgaaa ccaacgcgg aaataatcc tagagaaat 
      tatcaaacg ccttcttca aacaaccgt caaaagagg 
      ccgaatcaa caatttcca aatcgctga aattagtta 
      acgattttg tccaaagca ctacgtccg aaaagtcac 
      tcaaaaata tggcactgg atgcgatta aaagcataa 
      cgtgttcgc cgttgtacg caaaagtta gggccgagt 
      ttgaggata ttgtagtgg cagtgactg aaaaacatt 
      tggcgttta atagtccga attattttc agttgatgg 
      gacatttgg cacgaatgg ctcccgaga ctgtgaaaa 
      atataactc ctagagtta agcaacatt tggcgaatg 
      tggagagga tgcgagggg ttggcaatg atgcgtttc 
      tcgaccgaa ttttgcgcg atatggttg tgaaatacg 
      tgccgcatt ttgcaattg ccgactcgc caatgctca 
      aatgcctcg acaatgaaa ccaacgtgg aaataatcc 
      tagagaaat tatcaaacg ccttcttca aacaaccga 
      caaaagaag ccgaatcaa caatttcca aatcgctga 
      aattagtta acgattttg tccaaagca ctacgtccg 
      aaaagtcac taaagttgg ttgtagata aaacattgt 
      atggcctcc ttgaggtcg tgatgtttc acaatcgca 
      gaaatatgg cactggatg cgattaaaa gcataacgt 
      gttcgtcgt tgtacgcaa aagttaagg ccgagtttg 
      aggatattg tagtggcag tgactgaaa aacatttgg 
      cgtttaata gtccgaatt attttcagt tgatggggc 
      atttggcac gaatagctc ccgagactg tgaaaaata 
      taactccta gagttaagc aacatttgg cgaatgtgg 
      agaggatgc gaggggttg gcaatgatg cgtttctcg 
      accgaattt tgcgcgata tggttgtga aatacgtgc 
      cgcattttg caattgccg actcgccaa tgctcaaat 
      gcctcgaca atgaaacca acgtggaaa taatcctag 
      agaaattat caaacgcct tcttcaaac aaccgacaa 
      aagaagccg aatcaacaa tttccaaat cgctgaaat 
      tagttaacg attttgtcc aaagcacta cgtccgaaa 
      agtcactaa agttggttg tagataaaa cactgtatg 
      gcctccttg aggtcgtga tgtttcaca atcgcagaa 
      atatggcac tggatgcga ttaaaagca taacgtgtt 
      cgccgttgt acgcgaaag ttcgggccg agtttgagg 
      atactgtag tggcagtga ctgaaaaac atttggcgt 
      ttaatagtc cgaattatt ttcagttga tggggcatt 
      tggcacgaa tagctcccg agactgtga aaaatataa 
      ctcctagag ttaagcaac atttggcga atgtggaga 
      ggatgcgag gggttgggt atgataaaa atttgcggc 
      tgaatttgg aaagactgg aagcttcgc tgttacata 
      aatcttaat tgaatatta tttgtaagt aaattattg 
      taaatattt tttattggc gattttttt aattgaaaa 
      tatactatc caaaaatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY