Status
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Gene information
General information
TC032589
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010347 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24247509..24487403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032589
    iBeetle screen iB_04818

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032589 RA
      MAIENMYGT NYARLHVVV SSSKACNYH LSPTPGQSK 
MGSCGRGGT ASVWVTLLL TAALHPMAS TSHIKRSRH 
HRDSVTSDS PQTTHITLL ETNPCLVHY CTKGRECVV 
ENGEATCVC QKQCAVHRR LVCGSDGHV YPNHCELHR 
ASCMTATDI TVERGVHCI KHGKGWAPS VQHTNLYFN 
QFATAVPEQ ETHDATTGT YTQSIPEAV QVTPTKDAN 
FLLTSDVPP LPTRTEEAN YMENEIDYG REKGDGVLY 
DKEDCSMQE YEIMKDNLL LYNHARLMS QDNHSKDYL 
VSIMFSHYD SNNNGNLEA TELNEITAQ ERLDSLSSG 
CSLSNMIQY DDTDHDGKL SINEFYVAF SKLYSVSVV 
SLDKALEMN RLSARVGDN VEIKCDVTG SPVPPIVWR 
RNQLDLSTL NEEEIRVFS DGSLYLTRI QLQHAGNYT 
CHAQRNKDV VQTHILTVH TVPEVHVTP RIQSKRPGE 
DATMLCHVA GEPFPKVEW LKNDETLRL ENPRKYQVV 
GNGTALKIR NIAFADTGA YMCQATNIG GLTRDISSL 
VVQEQPTPT SPNEERRFF AFHDWGVSV YEPSTCRLY 
HRIQSTDII PGTQEYVCG DKGVKCSWG RAINVANRY 
VYASQPERD RVIIISKVQ MVVVDVVST DKYPVELFH 
VPHLDQVWI LNWRSTNNT GIKTIQVIR DAGQKRKHH 
TVHPESIDG HFDLVKGLF IPSPDQELS HYSFKYGYA 
THTNQRGLY KLDLVNLRY TRSVDLTPY NCVPAQIQF 
SALYGLVIM QCLEPVTSR PTGQLILDY LTDTVIGTK 
PNLPGIPYI SPDSRKLVT LDRTNSGAT LIVQEITEN 
GLVFSFDVK TTLNISDVT FYPSQTTHS YDIYASAQD 
KEDILFLNL LTGKVEIIT GVGKAIPMK LTKWNNPNR 
PISGSGLFG QYMVSPASE ALFVINGRS RTVNCEIGA 
VNHPNAIVW TTLKLH
    CDS sequence
    • >TC032589 RA
      atggcaata gagaacatg tatggaacg aattatgcc 
aggcttcat gtggtagtg tcttcgtcg aaggcttgt 
aactaccat ctaagtcct actcctgga cagagtaag 
atgggaagt tgcggtaga ggcggaaca gcctcggtg 
tgggtgact ttactccta acagcggcc cttcatcca 
atggccagc acttcacac attaagaga tcgaggcat 
cacagagat tcagtgaca tctgatagc ccccaaacg 
acacatatc acattactg gaaacaaat ccctgcctg 
gtgcattat tgtacgaag gggagagaa tgcgttgtc 
gaaaacggc gaagcgacg tgcgtctgt cagaagcaa 
tgtgccgtg catcggagg ttggtttgt ggctccgat 
ggccacgtt taccccaac cactgtgaa ttacacaga 
gcttcctgc atgaccgcc accgatatt accgtagaa 
agaggagtg cactgcatc aaacacgga aagggttgg 
gccccatcg gtgcagcac acaaactta tatttcaat 
cagttcgcc actgcggtc ccggaacag gaaacccat 
gatgccaca acgggaact tacacacaa agcattccg 
gaggccgtg caagtcaca ccaactaaa gatgccaac 
ttcctttta acttctgac gtgccacct ttgccaaca 
aggacggaa gaggcgaac tacatggaa aacgagatc 
gactacggg cgagaaaag ggagacggt gtgctctat 
gacaaagaa gactgctct atgcaggag tacgaaata 
atgaaagat aatttattg ttgtacaac catgccagg 
ctgatgtca caggacaac catagcaaa gattacttg 
gtttccatt atgttctcc cattatgac agcaacaac 
aatggtaac ctggaggcg accgaatta aacgagatc 
accgctcag gagcgtctg gatagcttg agctccggc 
tgttccctt tcaaatatg atccaatac gacgatacc 
gaccacgac ggcaaattg agcataaac gaattctac 
gtggctttt agcaaattg tacagtgta tcggtcgta 
tccctggat aaggcgctg gaaatgaac cgtttgagt 
gcccgagtg ggcgataac gtggaaatc aagtgtgac 
gttaccggt tcacctgtt ccaccgatc gtctggaga 
cggaatcag ttggacctg tccacttta aacgaagaa 
gaaatacga gtgttcagt gacggcagt ctttactta 
actaggata caactccag catgccgga aattacacc 
tgccatgct caaagaaac aaagacgtt gttcagacg 
cacatcctt actgttcat actgtgcca gaagtgcat 
gtcacgccc aggatccaa tcgaagagg ccaggggag 
gatgccacc atgctgtgc catgtggcg ggagagccc 
tttcccaag gtagagtgg ctgaaaaat gacgaaacc 
ctccgatta gaaaatccc cgcaaatac caagtagtg 
ggaaacgga accgctctg aaaataagg aacatagct 
ttcgctgat acgggggct tacatgtgc caagccact 
aatataggt ggattaaca agagacata agttctctg 
gtcgtccaa gagcagccg acacctacc tcgcctaac 
gaagagcgg cgctttttt gccttccac gactgggga 
gtgtcagtg tatgagcca tccacttgt cgactctac 
cacaggatt caatccaca gacatcatt ccaggcact 
caggaatac gtgtgtggc gataaagga gtaaaatgc 
tcctgggga cgtgccata aacgttgcc aaccgatac 
gtctacgcc agccaacca gaaagggac cgagtaata 
atcatttcc aaagtacaa atggtggta gtagacgtt 
gttagcacc gacaaatat cccgtggaa ctcttccac 
gtccctcat ttagaccaa gtgtggata cttaactgg 
agatcaacg aacaatacc ggaattaaa accattcag 
gtgattagg gacgcaggc cagaagcga aaacaccac 
actgtgcat ccggaatca atcgacggt cattttgat 
ctagttaag gggcttttt attccgtct ccggaccaa 
gagctttcg cattatagc ttcaaatac gggtatgca 
acccacacc aatcaacga ggtctctac aaactggat 
ctcgttaat ttgagatac accagatca gttgatctc 
acaccctac aactgcgtt ccagcccag atccaattt 
tccgcgtta tacggtttg gtgataatg cagtgtctg 
gagcccgtc actagcagg cccaccggt caattgatt 
ctggattat ctgacagat acggttatt ggaacgaaa 
ccaaatttg cctggaatc ccgtacata tctcccgat 
tctaggaaa ctcgttaca ctagatcgt acaaatagc 
ggagccact cttatcgtg caagagata acagagaac 
ggtcttgtg ttttcgttt gatgtgaaa acaacgctg 
aatatatcc gatgtcacg ttctatccg agtcaaaca 
actcacagt tatgatatt tacgcaagt gcccaagac 
aaggaagat attctattc ctcaatctg ttaacaggg 
aaagtggag atcataacg ggtgtcggg aaagcaatt 
cccatgaaa ttaaccaaa tggaacaat cccaacaga 
cctatcagc ggatcaggt ttgttcggg cagtacatg 
gtgtccccc gccagtgag gcgttattc gtgataaac 
gggcggtcg cgaacagta aattgtgaa atcggtgca 
gtgaaccac ccgaacgcc atcgtctgg accaccctc 
aagctccat tag
    mRNA sequence
    • >TC032589 RA
      ggggtattt taccacata ggattgcat tgtcacgca 
gattattag atggcaata gagaacatg tatggaacg 
aattatgcc aggcttcat gtggtagtg tcttcgtcg 
aaggcttgt aactaccat ctaagtcct actcctgga 
cagagtaag atgggaagt tgcggtaga ggcggaaca 
gcctcggtg tgggtgact ttactccta acagcggcc 
cttcatcca atggccagc acttcacac attaagaga 
tcgaggcat cacagagat tcagtgaca tctgatagc 
ccccaaacg acacatatc acattactg gaaacaaat 
ccctgcctg gtgcattat tgtacgaag gggagagaa 
tgcgttgtc gaaaacggc gaagcgacg tgcgtctgt 
cagaagcaa tgtgccgtg catcggagg ttggtttgt 
ggctccgat ggccacgtt taccccaac cactgtgaa 
ttacacaga gcttcctgc atgaccgcc accgatatt 
accgtagaa agaggagtg cactgcatc aaacacgga 
aagggttgg gccccatcg gtgcagcac acaaactta 
tatttcaat cagttcgcc actgcggtc ccggaacag 
gaaacccat gatgccaca acgggaact tacacacaa 
agcattccg gaggccgtg caagtcaca ccaactaaa 
gatgccaac ttcctttta acttctgac gtgccacct 
ttgccaaca aggacggaa gaggcgaac tacatggaa 
aacgagatc gactacggg cgagaaaag ggagacggt 
gtgctctat gacaaagaa gactgctct atgcaggag 
tacgaaata atgaaagat aatttattg ttgtacaac 
catgccagg ctgatgtca caggacaac catagcaaa 
gattacttg gtttccatt atgttctcc cattatgac 
agcaacaac aatggtaac ctggaggcg accgaatta 
aacgagatc accgctcag gagcgtctg gatagcttg 
agctccggc tgttccctt tcaaatatg atccaatac 
gacgatacc gaccacgac ggcaaattg agcataaac 
gaattctac gtggctttt agcaaattg tacagtgta 
tcggtcgta tccctggat aaggcgctg gaaatgaac 
cgtttgagt gcccgagtg ggcgataac gtggaaatc 
aagtgtgac gttaccggt tcacctgtt ccaccgatc 
gtctggaga cggaatcag ttggacctg tccacttta 
aacgaagaa gaaatacga gtgttcagt gacggcagt 
ctttactta actaggata caactccag catgccgga 
aattacacc tgccatgct caaagaaac aaagacgtt 
gttcagacg cacatcctt actgttcat actgtgcca 
gaagtgcat gtcacgccc aggatccaa tcgaagagg 
ccaggggag gatgccacc atgctgtgc catgtggcg 
ggagagccc tttcccaag gtagagtgg ctgaaaaat 
gacgaaacc ctccgatta gaaaatccc cgcaaatac 
caagtagtg ggaaacgga accgctctg aaaataagg 
aacatagct ttcgctgat acgggggct tacatgtgc 
caagccact aatataggt ggattaaca agagacata 
agttctctg gtcgtccaa gagcagccg acacctacc 
tcgcctaac gaagagcgg cgctttttt gccttccac 
gactgggga gtgtcagtg tatgagcca tccacttgt 
cgactctac cacaggatt caatccaca gacatcatt 
ccaggcact caggaatac gtgtgtggc gataaagga 
gtaaaatgc tcctgggga cgtgccata aacgttgcc 
aaccgatac gtctacgcc agccaacca gaaagggac 
cgagtaata atcatttcc aaagtacaa atggtggta 
gtagacgtt gttagcacc gacaaatat cccgtggaa 
ctcttccac gtccctcat ttagaccaa gtgtggata 
cttaactgg agatcaacg aacaatacc ggaattaaa 
accattcag gtgattagg gacgcaggc cagaagcga 
aaacaccac actgtgcat ccggaatca atcgacggt 
cattttgat ctagttaag gggcttttt attccgtct 
ccggaccaa gagctttcg cattatagc ttcaaatac 
gggtatgca acccacacc aatcaacga ggtctctac 
aaactggat ctcgttaat ttgagatac accagatca 
gttgatctc acaccctac aactgcgtt ccagcccag 
atccaattt tccgcgtta tacggtttg gtgataatg 
cagtgtctg gagcccgtc actagcagg cccaccggt 
caattgatt ctggattat ctgacagat acggttatt 
ggaacgaaa ccaaatttg cctggaatc ccgtacata 
tctcccgat tctaggaaa ctcgttaca ctagatcgt 
acaaatagc ggagccact cttatcgtg caagagata 
acagagaac ggtcttgtg ttttcgttt gatgtgaaa 
acaacgctg aatatatcc gatgtcacg ttctatccg 
agtcaaaca actcacagt tatgatatt tacgcaagt 
gcccaagac aaggaagat attctattc ctcaatctg 
ttaacaggg aaagtggag atcataacg ggtgtcggg 
aaagcaatt cccatgaaa ttaaccaaa tggaacaat 
cccaacaga cctatcagc ggatcaggt ttgttcggg 
cagtacatg gtgtccccc gccagtgag gcgttattc 
gtgataaac gggcggtcg cgaacagta aattgtgaa 
atcggtgca gtgaaccac ccgaacgcc atcgtctgg 
accaccctc aagctccat tagtggtcg gccaaacaa 
atatcaact cgtttcgta ttacttaaa aattatact 
ttttatact aatatcaaa accgctgcg tgtcaaacc 
catcacagc atggctttg acacgaaga tagtttagg 
aaataataa ttgttaaga atgtattgt cagacctac 
tgttgtagc caagtctac gattatcaa tatcgagat 
cactacatc tgtccaatt tttggtgac tctgatttg 
gaactgaat attttaata ataacgttg ttttagtaa 
atgtagtct tgtgataac ttattgagt ccccacaac 
aacaccacc gccaattgt atttcattc tcgacgcat 
agattacgc acacgatat tttttaaac gaaatagtt 
tcgggagat gttacgcag ccactgtgg ggatgtaat 
acacttgga tgggagttt ttcgtgatt gacttgatt 
tcgttcgca gaacttgga cctttcttt cgaccactt 
tgttgtagc ttaactttt ccgtaggtt tcatgtagg 
atgtactct tgtttcggt ttttgctag cttgctctc 
tatatctcg cctttatat aagctaaaa tgttgttta 
tcgtatcac aaatgggct tattgtaat ataggtatt 
atggtacaa tattgttta tacagaaat aaactgaca 
acattgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY