| General information | |||
| TC032589
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 24247509..24487403 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032589 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04818 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC032589 RA
MAIENMYGT NYARLHVVV SSSKACNYH LSPTPGQSK MGSCGRGGT ASVWVTLLL TAALHPMAS TSHIKRSRH HRDSVTSDS PQTTHITLL ETNPCLVHY CTKGRECVV ENGEATCVC QKQCAVHRR LVCGSDGHV YPNHCELHR ASCMTATDI TVERGVHCI KHGKGWAPS VQHTNLYFN QFATAVPEQ ETHDATTGT YTQSIPEAV QVTPTKDAN FLLTSDVPP LPTRTEEAN YMENEIDYG REKGDGVLY DKEDCSMQE YEIMKDNLL LYNHARLMS QDNHSKDYL VSIMFSHYD SNNNGNLEA TELNEITAQ ERLDSLSSG CSLSNMIQY DDTDHDGKL SINEFYVAF SKLYSVSVV SLDKALEMN RLSARVGDN VEIKCDVTG SPVPPIVWR RNQLDLSTL NEEEIRVFS DGSLYLTRI QLQHAGNYT CHAQRNKDV VQTHILTVH TVPEVHVTP RIQSKRPGE DATMLCHVA GEPFPKVEW LKNDETLRL ENPRKYQVV GNGTALKIR NIAFADTGA YMCQATNIG GLTRDISSL VVQEQPTPT SPNEERRFF AFHDWGVSV YEPSTCRLY HRIQSTDII PGTQEYVCG DKGVKCSWG RAINVANRY VYASQPERD RVIIISKVQ MVVVDVVST DKYPVELFH VPHLDQVWI LNWRSTNNT GIKTIQVIR DAGQKRKHH TVHPESIDG HFDLVKGLF IPSPDQELS HYSFKYGYA THTNQRGLY KLDLVNLRY TRSVDLTPY NCVPAQIQF SALYGLVIM QCLEPVTSR PTGQLILDY LTDTVIGTK PNLPGIPYI SPDSRKLVT LDRTNSGAT LIVQEITEN GLVFSFDVK TTLNISDVT FYPSQTTHS YDIYASAQD KEDILFLNL LTGKVEIIT GVGKAIPMK LTKWNNPNR PISGSGLFG QYMVSPASE ALFVINGRS RTVNCEIGA VNHPNAIVW TTLKLH
- >TC032589 RA
atggcaata gagaacatg tatggaacg aattatgcc aggcttcat gtggtagtg tcttcgtcg aaggcttgt aactaccat ctaagtcct actcctgga cagagtaag atgggaagt tgcggtaga ggcggaaca gcctcggtg tgggtgact ttactccta acagcggcc cttcatcca atggccagc acttcacac attaagaga tcgaggcat cacagagat tcagtgaca tctgatagc ccccaaacg acacatatc acattactg gaaacaaat ccctgcctg gtgcattat tgtacgaag gggagagaa tgcgttgtc gaaaacggc gaagcgacg tgcgtctgt cagaagcaa tgtgccgtg catcggagg ttggtttgt ggctccgat ggccacgtt taccccaac cactgtgaa ttacacaga gcttcctgc atgaccgcc accgatatt accgtagaa agaggagtg cactgcatc aaacacgga aagggttgg gccccatcg gtgcagcac acaaactta tatttcaat cagttcgcc actgcggtc ccggaacag gaaacccat gatgccaca acgggaact tacacacaa agcattccg gaggccgtg caagtcaca ccaactaaa gatgccaac ttcctttta acttctgac gtgccacct ttgccaaca aggacggaa gaggcgaac tacatggaa aacgagatc gactacggg cgagaaaag ggagacggt gtgctctat gacaaagaa gactgctct atgcaggag tacgaaata atgaaagat aatttattg ttgtacaac catgccagg ctgatgtca caggacaac catagcaaa gattacttg gtttccatt atgttctcc cattatgac agcaacaac aatggtaac ctggaggcg accgaatta aacgagatc accgctcag gagcgtctg gatagcttg agctccggc tgttccctt tcaaatatg atccaatac gacgatacc gaccacgac ggcaaattg agcataaac gaattctac gtggctttt agcaaattg tacagtgta tcggtcgta tccctggat aaggcgctg gaaatgaac cgtttgagt gcccgagtg ggcgataac gtggaaatc aagtgtgac gttaccggt tcacctgtt ccaccgatc gtctggaga cggaatcag ttggacctg tccacttta aacgaagaa gaaatacga gtgttcagt gacggcagt ctttactta actaggata caactccag catgccgga aattacacc tgccatgct caaagaaac aaagacgtt gttcagacg cacatcctt actgttcat actgtgcca gaagtgcat gtcacgccc aggatccaa tcgaagagg ccaggggag gatgccacc atgctgtgc catgtggcg ggagagccc tttcccaag gtagagtgg ctgaaaaat gacgaaacc ctccgatta gaaaatccc cgcaaatac caagtagtg ggaaacgga accgctctg aaaataagg aacatagct ttcgctgat acgggggct tacatgtgc caagccact aatataggt ggattaaca agagacata agttctctg gtcgtccaa gagcagccg acacctacc tcgcctaac gaagagcgg cgctttttt gccttccac gactgggga gtgtcagtg tatgagcca tccacttgt cgactctac cacaggatt caatccaca gacatcatt ccaggcact caggaatac gtgtgtggc gataaagga gtaaaatgc tcctgggga cgtgccata aacgttgcc aaccgatac gtctacgcc agccaacca gaaagggac cgagtaata atcatttcc aaagtacaa atggtggta gtagacgtt gttagcacc gacaaatat cccgtggaa ctcttccac gtccctcat ttagaccaa gtgtggata cttaactgg agatcaacg aacaatacc ggaattaaa accattcag gtgattagg gacgcaggc cagaagcga aaacaccac actgtgcat ccggaatca atcgacggt cattttgat ctagttaag gggcttttt attccgtct ccggaccaa gagctttcg cattatagc ttcaaatac gggtatgca acccacacc aatcaacga ggtctctac aaactggat ctcgttaat ttgagatac accagatca gttgatctc acaccctac aactgcgtt ccagcccag atccaattt tccgcgtta tacggtttg gtgataatg cagtgtctg gagcccgtc actagcagg cccaccggt caattgatt ctggattat ctgacagat acggttatt ggaacgaaa ccaaatttg cctggaatc ccgtacata tctcccgat tctaggaaa ctcgttaca ctagatcgt acaaatagc ggagccact cttatcgtg caagagata acagagaac ggtcttgtg ttttcgttt gatgtgaaa acaacgctg aatatatcc gatgtcacg ttctatccg agtcaaaca actcacagt tatgatatt tacgcaagt gcccaagac aaggaagat attctattc ctcaatctg ttaacaggg aaagtggag atcataacg ggtgtcggg aaagcaatt cccatgaaa ttaaccaaa tggaacaat cccaacaga cctatcagc ggatcaggt ttgttcggg cagtacatg gtgtccccc gccagtgag gcgttattc gtgataaac gggcggtcg cgaacagta aattgtgaa atcggtgca gtgaaccac ccgaacgcc atcgtctgg accaccctc aagctccat tag
- >TC032589 RA
ggggtattt taccacata ggattgcat tgtcacgca gattattag atggcaata gagaacatg tatggaacg aattatgcc aggcttcat gtggtagtg tcttcgtcg aaggcttgt aactaccat ctaagtcct actcctgga cagagtaag atgggaagt tgcggtaga ggcggaaca gcctcggtg tgggtgact ttactccta acagcggcc cttcatcca atggccagc acttcacac attaagaga tcgaggcat cacagagat tcagtgaca tctgatagc ccccaaacg acacatatc acattactg gaaacaaat ccctgcctg gtgcattat tgtacgaag gggagagaa tgcgttgtc gaaaacggc gaagcgacg tgcgtctgt cagaagcaa tgtgccgtg catcggagg ttggtttgt ggctccgat ggccacgtt taccccaac cactgtgaa ttacacaga gcttcctgc atgaccgcc accgatatt accgtagaa agaggagtg cactgcatc aaacacgga aagggttgg gccccatcg gtgcagcac acaaactta tatttcaat cagttcgcc actgcggtc ccggaacag gaaacccat gatgccaca acgggaact tacacacaa agcattccg gaggccgtg caagtcaca ccaactaaa gatgccaac ttcctttta acttctgac gtgccacct ttgccaaca aggacggaa gaggcgaac tacatggaa aacgagatc gactacggg cgagaaaag ggagacggt gtgctctat gacaaagaa gactgctct atgcaggag tacgaaata atgaaagat aatttattg ttgtacaac catgccagg ctgatgtca caggacaac catagcaaa gattacttg gtttccatt atgttctcc cattatgac agcaacaac aatggtaac ctggaggcg accgaatta aacgagatc accgctcag gagcgtctg gatagcttg agctccggc tgttccctt tcaaatatg atccaatac gacgatacc gaccacgac ggcaaattg agcataaac gaattctac gtggctttt agcaaattg tacagtgta tcggtcgta tccctggat aaggcgctg gaaatgaac cgtttgagt gcccgagtg ggcgataac gtggaaatc aagtgtgac gttaccggt tcacctgtt ccaccgatc gtctggaga cggaatcag ttggacctg tccacttta aacgaagaa gaaatacga gtgttcagt gacggcagt ctttactta actaggata caactccag catgccgga aattacacc tgccatgct caaagaaac aaagacgtt gttcagacg cacatcctt actgttcat actgtgcca gaagtgcat gtcacgccc aggatccaa tcgaagagg ccaggggag gatgccacc atgctgtgc catgtggcg ggagagccc tttcccaag gtagagtgg ctgaaaaat gacgaaacc ctccgatta gaaaatccc cgcaaatac caagtagtg ggaaacgga accgctctg aaaataagg aacatagct ttcgctgat acgggggct tacatgtgc caagccact aatataggt ggattaaca agagacata agttctctg gtcgtccaa gagcagccg acacctacc tcgcctaac gaagagcgg cgctttttt gccttccac gactgggga gtgtcagtg tatgagcca tccacttgt cgactctac cacaggatt caatccaca gacatcatt ccaggcact caggaatac gtgtgtggc gataaagga gtaaaatgc tcctgggga cgtgccata aacgttgcc aaccgatac gtctacgcc agccaacca gaaagggac cgagtaata atcatttcc aaagtacaa atggtggta gtagacgtt gttagcacc gacaaatat cccgtggaa ctcttccac gtccctcat ttagaccaa gtgtggata cttaactgg agatcaacg aacaatacc ggaattaaa accattcag gtgattagg gacgcaggc cagaagcga aaacaccac actgtgcat ccggaatca atcgacggt cattttgat ctagttaag gggcttttt attccgtct ccggaccaa gagctttcg cattatagc ttcaaatac gggtatgca acccacacc aatcaacga ggtctctac aaactggat ctcgttaat ttgagatac accagatca gttgatctc acaccctac aactgcgtt ccagcccag atccaattt tccgcgtta tacggtttg gtgataatg cagtgtctg gagcccgtc actagcagg cccaccggt caattgatt ctggattat ctgacagat acggttatt ggaacgaaa ccaaatttg cctggaatc ccgtacata tctcccgat tctaggaaa ctcgttaca ctagatcgt acaaatagc ggagccact cttatcgtg caagagata acagagaac ggtcttgtg ttttcgttt gatgtgaaa acaacgctg aatatatcc gatgtcacg ttctatccg agtcaaaca actcacagt tatgatatt tacgcaagt gcccaagac aaggaagat attctattc ctcaatctg ttaacaggg aaagtggag atcataacg ggtgtcggg aaagcaatt cccatgaaa ttaaccaaa tggaacaat cccaacaga cctatcagc ggatcaggt ttgttcggg cagtacatg gtgtccccc gccagtgag gcgttattc gtgataaac gggcggtcg cgaacagta aattgtgaa atcggtgca gtgaaccac ccgaacgcc atcgtctgg accaccctc aagctccat tagtggtcg gccaaacaa atatcaact cgtttcgta ttacttaaa aattatact ttttatact aatatcaaa accgctgcg tgtcaaacc catcacagc atggctttg acacgaaga tagtttagg aaataataa ttgttaaga atgtattgt cagacctac tgttgtagc caagtctac gattatcaa tatcgagat cactacatc tgtccaatt tttggtgac tctgatttg gaactgaat attttaata ataacgttg ttttagtaa atgtagtct tgtgataac ttattgagt ccccacaac aacaccacc gccaattgt atttcattc tcgacgcat agattacgc acacgatat tttttaaac gaaatagtt tcgggagat gttacgcag ccactgtgg ggatgtaat acacttgga tgggagttt ttcgtgatt gacttgatt tcgttcgca gaacttgga cctttcttt cgaccactt tgttgtagc ttaactttt ccgtaggtt tcatgtagg atgtactct tgtttcggt ttttgctag cttgctctc tatatctcg cctttatat aagctaaaa tgttgttta tcgtatcac aaatgggct tattgtaat ataggtatt atggtacaa tattgttta tacagaaat aaactgaca acattgaa
