Status
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Gene information
General information
TC032589
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010347
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24247509..24487403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032589
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04818

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032589 RA
      MAIENMYGT NYARLHVVV SSSKACNYH LSPTPGQSK 
      MGSCGRGGT ASVWVTLLL TAALHPMAS TSHIKRSRH 
      HRDSVTSDS PQTTHITLL ETNPCLVHY CTKGRECVV 
      ENGEATCVC QKQCAVHRR LVCGSDGHV YPNHCELHR 
      ASCMTATDI TVERGVHCI KHGKGWAPS VQHTNLYFN 
      QFATAVPEQ ETHDATTGT YTQSIPEAV QVTPTKDAN 
      FLLTSDVPP LPTRTEEAN YMENEIDYG REKGDGVLY 
      DKEDCSMQE YEIMKDNLL LYNHARLMS QDNHSKDYL 
      VSIMFSHYD SNNNGNLEA TELNEITAQ ERLDSLSSG 
      CSLSNMIQY DDTDHDGKL SINEFYVAF SKLYSVSVV 
      SLDKALEMN RLSARVGDN VEIKCDVTG SPVPPIVWR 
      RNQLDLSTL NEEEIRVFS DGSLYLTRI QLQHAGNYT 
      CHAQRNKDV VQTHILTVH TVPEVHVTP RIQSKRPGE 
      DATMLCHVA GEPFPKVEW LKNDETLRL ENPRKYQVV 
      GNGTALKIR NIAFADTGA YMCQATNIG GLTRDISSL 
      VVQEQPTPT SPNEERRFF AFHDWGVSV YEPSTCRLY 
      HRIQSTDII PGTQEYVCG DKGVKCSWG RAINVANRY 
      VYASQPERD RVIIISKVQ MVVVDVVST DKYPVELFH 
      VPHLDQVWI LNWRSTNNT GIKTIQVIR DAGQKRKHH 
      TVHPESIDG HFDLVKGLF IPSPDQELS HYSFKYGYA 
      THTNQRGLY KLDLVNLRY TRSVDLTPY NCVPAQIQF 
      SALYGLVIM QCLEPVTSR PTGQLILDY LTDTVIGTK 
      PNLPGIPYI SPDSRKLVT LDRTNSGAT LIVQEITEN 
      GLVFSFDVK TTLNISDVT FYPSQTTHS YDIYASAQD 
      KEDILFLNL LTGKVEIIT GVGKAIPMK LTKWNNPNR 
      PISGSGLFG QYMVSPASE ALFVINGRS RTVNCEIGA 
      VNHPNAIVW TTLKLH
    CDS sequence
    • >TC032589 RA
      atggcaata gagaacatg tatggaacg aattatgcc 
      aggcttcat gtggtagtg tcttcgtcg aaggcttgt 
      aactaccat ctaagtcct actcctgga cagagtaag 
      atgggaagt tgcggtaga ggcggaaca gcctcggtg 
      tgggtgact ttactccta acagcggcc cttcatcca 
      atggccagc acttcacac attaagaga tcgaggcat 
      cacagagat tcagtgaca tctgatagc ccccaaacg 
      acacatatc acattactg gaaacaaat ccctgcctg 
      gtgcattat tgtacgaag gggagagaa tgcgttgtc 
      gaaaacggc gaagcgacg tgcgtctgt cagaagcaa 
      tgtgccgtg catcggagg ttggtttgt ggctccgat 
      ggccacgtt taccccaac cactgtgaa ttacacaga 
      gcttcctgc atgaccgcc accgatatt accgtagaa 
      agaggagtg cactgcatc aaacacgga aagggttgg 
      gccccatcg gtgcagcac acaaactta tatttcaat 
      cagttcgcc actgcggtc ccggaacag gaaacccat 
      gatgccaca acgggaact tacacacaa agcattccg 
      gaggccgtg caagtcaca ccaactaaa gatgccaac 
      ttcctttta acttctgac gtgccacct ttgccaaca 
      aggacggaa gaggcgaac tacatggaa aacgagatc 
      gactacggg cgagaaaag ggagacggt gtgctctat 
      gacaaagaa gactgctct atgcaggag tacgaaata 
      atgaaagat aatttattg ttgtacaac catgccagg 
      ctgatgtca caggacaac catagcaaa gattacttg 
      gtttccatt atgttctcc cattatgac agcaacaac 
      aatggtaac ctggaggcg accgaatta aacgagatc 
      accgctcag gagcgtctg gatagcttg agctccggc 
      tgttccctt tcaaatatg atccaatac gacgatacc 
      gaccacgac ggcaaattg agcataaac gaattctac 
      gtggctttt agcaaattg tacagtgta tcggtcgta 
      tccctggat aaggcgctg gaaatgaac cgtttgagt 
      gcccgagtg ggcgataac gtggaaatc aagtgtgac 
      gttaccggt tcacctgtt ccaccgatc gtctggaga 
      cggaatcag ttggacctg tccacttta aacgaagaa 
      gaaatacga gtgttcagt gacggcagt ctttactta 
      actaggata caactccag catgccgga aattacacc 
      tgccatgct caaagaaac aaagacgtt gttcagacg 
      cacatcctt actgttcat actgtgcca gaagtgcat 
      gtcacgccc aggatccaa tcgaagagg ccaggggag 
      gatgccacc atgctgtgc catgtggcg ggagagccc 
      tttcccaag gtagagtgg ctgaaaaat gacgaaacc 
      ctccgatta gaaaatccc cgcaaatac caagtagtg 
      ggaaacgga accgctctg aaaataagg aacatagct 
      ttcgctgat acgggggct tacatgtgc caagccact 
      aatataggt ggattaaca agagacata agttctctg 
      gtcgtccaa gagcagccg acacctacc tcgcctaac 
      gaagagcgg cgctttttt gccttccac gactgggga 
      gtgtcagtg tatgagcca tccacttgt cgactctac 
      cacaggatt caatccaca gacatcatt ccaggcact 
      caggaatac gtgtgtggc gataaagga gtaaaatgc 
      tcctgggga cgtgccata aacgttgcc aaccgatac 
      gtctacgcc agccaacca gaaagggac cgagtaata 
      atcatttcc aaagtacaa atggtggta gtagacgtt 
      gttagcacc gacaaatat cccgtggaa ctcttccac 
      gtccctcat ttagaccaa gtgtggata cttaactgg 
      agatcaacg aacaatacc ggaattaaa accattcag 
      gtgattagg gacgcaggc cagaagcga aaacaccac 
      actgtgcat ccggaatca atcgacggt cattttgat 
      ctagttaag gggcttttt attccgtct ccggaccaa 
      gagctttcg cattatagc ttcaaatac gggtatgca 
      acccacacc aatcaacga ggtctctac aaactggat 
      ctcgttaat ttgagatac accagatca gttgatctc 
      acaccctac aactgcgtt ccagcccag atccaattt 
      tccgcgtta tacggtttg gtgataatg cagtgtctg 
      gagcccgtc actagcagg cccaccggt caattgatt 
      ctggattat ctgacagat acggttatt ggaacgaaa 
      ccaaatttg cctggaatc ccgtacata tctcccgat 
      tctaggaaa ctcgttaca ctagatcgt acaaatagc 
      ggagccact cttatcgtg caagagata acagagaac 
      ggtcttgtg ttttcgttt gatgtgaaa acaacgctg 
      aatatatcc gatgtcacg ttctatccg agtcaaaca 
      actcacagt tatgatatt tacgcaagt gcccaagac 
      aaggaagat attctattc ctcaatctg ttaacaggg 
      aaagtggag atcataacg ggtgtcggg aaagcaatt 
      cccatgaaa ttaaccaaa tggaacaat cccaacaga 
      cctatcagc ggatcaggt ttgttcggg cagtacatg 
      gtgtccccc gccagtgag gcgttattc gtgataaac 
      gggcggtcg cgaacagta aattgtgaa atcggtgca 
      gtgaaccac ccgaacgcc atcgtctgg accaccctc 
      aagctccat tag
    mRNA sequence
    • >TC032589 RA
      ggggtattt taccacata ggattgcat tgtcacgca 
      gattattag atggcaata gagaacatg tatggaacg 
      aattatgcc aggcttcat gtggtagtg tcttcgtcg 
      aaggcttgt aactaccat ctaagtcct actcctgga 
      cagagtaag atgggaagt tgcggtaga ggcggaaca 
      gcctcggtg tgggtgact ttactccta acagcggcc 
      cttcatcca atggccagc acttcacac attaagaga 
      tcgaggcat cacagagat tcagtgaca tctgatagc 
      ccccaaacg acacatatc acattactg gaaacaaat 
      ccctgcctg gtgcattat tgtacgaag gggagagaa 
      tgcgttgtc gaaaacggc gaagcgacg tgcgtctgt 
      cagaagcaa tgtgccgtg catcggagg ttggtttgt 
      ggctccgat ggccacgtt taccccaac cactgtgaa 
      ttacacaga gcttcctgc atgaccgcc accgatatt 
      accgtagaa agaggagtg cactgcatc aaacacgga 
      aagggttgg gccccatcg gtgcagcac acaaactta 
      tatttcaat cagttcgcc actgcggtc ccggaacag 
      gaaacccat gatgccaca acgggaact tacacacaa 
      agcattccg gaggccgtg caagtcaca ccaactaaa 
      gatgccaac ttcctttta acttctgac gtgccacct 
      ttgccaaca aggacggaa gaggcgaac tacatggaa 
      aacgagatc gactacggg cgagaaaag ggagacggt 
      gtgctctat gacaaagaa gactgctct atgcaggag 
      tacgaaata atgaaagat aatttattg ttgtacaac 
      catgccagg ctgatgtca caggacaac catagcaaa 
      gattacttg gtttccatt atgttctcc cattatgac 
      agcaacaac aatggtaac ctggaggcg accgaatta 
      aacgagatc accgctcag gagcgtctg gatagcttg 
      agctccggc tgttccctt tcaaatatg atccaatac 
      gacgatacc gaccacgac ggcaaattg agcataaac 
      gaattctac gtggctttt agcaaattg tacagtgta 
      tcggtcgta tccctggat aaggcgctg gaaatgaac 
      cgtttgagt gcccgagtg ggcgataac gtggaaatc 
      aagtgtgac gttaccggt tcacctgtt ccaccgatc 
      gtctggaga cggaatcag ttggacctg tccacttta 
      aacgaagaa gaaatacga gtgttcagt gacggcagt 
      ctttactta actaggata caactccag catgccgga 
      aattacacc tgccatgct caaagaaac aaagacgtt 
      gttcagacg cacatcctt actgttcat actgtgcca 
      gaagtgcat gtcacgccc aggatccaa tcgaagagg 
      ccaggggag gatgccacc atgctgtgc catgtggcg 
      ggagagccc tttcccaag gtagagtgg ctgaaaaat 
      gacgaaacc ctccgatta gaaaatccc cgcaaatac 
      caagtagtg ggaaacgga accgctctg aaaataagg 
      aacatagct ttcgctgat acgggggct tacatgtgc 
      caagccact aatataggt ggattaaca agagacata 
      agttctctg gtcgtccaa gagcagccg acacctacc 
      tcgcctaac gaagagcgg cgctttttt gccttccac 
      gactgggga gtgtcagtg tatgagcca tccacttgt 
      cgactctac cacaggatt caatccaca gacatcatt 
      ccaggcact caggaatac gtgtgtggc gataaagga 
      gtaaaatgc tcctgggga cgtgccata aacgttgcc 
      aaccgatac gtctacgcc agccaacca gaaagggac 
      cgagtaata atcatttcc aaagtacaa atggtggta 
      gtagacgtt gttagcacc gacaaatat cccgtggaa 
      ctcttccac gtccctcat ttagaccaa gtgtggata 
      cttaactgg agatcaacg aacaatacc ggaattaaa 
      accattcag gtgattagg gacgcaggc cagaagcga 
      aaacaccac actgtgcat ccggaatca atcgacggt 
      cattttgat ctagttaag gggcttttt attccgtct 
      ccggaccaa gagctttcg cattatagc ttcaaatac 
      gggtatgca acccacacc aatcaacga ggtctctac 
      aaactggat ctcgttaat ttgagatac accagatca 
      gttgatctc acaccctac aactgcgtt ccagcccag 
      atccaattt tccgcgtta tacggtttg gtgataatg 
      cagtgtctg gagcccgtc actagcagg cccaccggt 
      caattgatt ctggattat ctgacagat acggttatt 
      ggaacgaaa ccaaatttg cctggaatc ccgtacata 
      tctcccgat tctaggaaa ctcgttaca ctagatcgt 
      acaaatagc ggagccact cttatcgtg caagagata 
      acagagaac ggtcttgtg ttttcgttt gatgtgaaa 
      acaacgctg aatatatcc gatgtcacg ttctatccg 
      agtcaaaca actcacagt tatgatatt tacgcaagt 
      gcccaagac aaggaagat attctattc ctcaatctg 
      ttaacaggg aaagtggag atcataacg ggtgtcggg 
      aaagcaatt cccatgaaa ttaaccaaa tggaacaat 
      cccaacaga cctatcagc ggatcaggt ttgttcggg 
      cagtacatg gtgtccccc gccagtgag gcgttattc 
      gtgataaac gggcggtcg cgaacagta aattgtgaa 
      atcggtgca gtgaaccac ccgaacgcc atcgtctgg 
      accaccctc aagctccat tagtggtcg gccaaacaa 
      atatcaact cgtttcgta ttacttaaa aattatact 
      ttttatact aatatcaaa accgctgcg tgtcaaacc 
      catcacagc atggctttg acacgaaga tagtttagg 
      aaataataa ttgttaaga atgtattgt cagacctac 
      tgttgtagc caagtctac gattatcaa tatcgagat 
      cactacatc tgtccaatt tttggtgac tctgatttg 
      gaactgaat attttaata ataacgttg ttttagtaa 
      atgtagtct tgtgataac ttattgagt ccccacaac 
      aacaccacc gccaattgt atttcattc tcgacgcat 
      agattacgc acacgatat tttttaaac gaaatagtt 
      tcgggagat gttacgcag ccactgtgg ggatgtaat 
      acacttgga tgggagttt ttcgtgatt gacttgatt 
      tcgttcgca gaacttgga cctttcttt cgaccactt 
      tgttgtagc ttaactttt ccgtaggtt tcatgtagg 
      atgtactct tgtttcggt ttttgctag cttgctctc 
      tatatctcg cctttatat aagctaaaa tgttgttta 
      tcgtatcac aaatgggct tattgtaat ataggtatt 
      atggtacaa tattgttta tacagaaat aaactgaca 
      acattgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY