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Gene information
General information
TC010343 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24648469..24668756
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010343
    iBeetle screen iB_04817

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010343 RA
      MDSGLGSDE DRRGRTKEQ KQLRNQQLL SGCFIDAAS 
      LSDDAEVDQ RRTDERRQG ALIFQSSIP QFSMLQMAG 
      NEAAPTYPE TSTPYNSIV HLEEDASRK SPLGFYVDL 
      NEVPDPPKT PPPATAAKK NIFSMVIDF EGPKKDKPT 
      KLSSSLVSY KKNRQSKQL TLTTRYNGN SSLSSSVSS 
      ISSVAGPSR QVEDEKEEV EGQNQMSAS SKSSSSSEN 
      FPMHKSEDE GHVEEHKPA EDDVVEKKC EETNAKNEE 
      IKEVETQER PQFVKLSDL ECQKPRLDL NFTPRMSKS 
      IPESSWVES PLLMSRSAG YRSAPHPLE PESGEFSDS 
      GSATDTSVS QPRRSVRRL GTDLLRMFL EEIGPDVTV 
      EVDGHKLKA HKCILASRC QYFAALLSG NWLEASGNI 
      ISLQGFSYE SVYFALCHI YSGAAHVPD SISLVELAS 
      LADMLGLEG LKEVVEHAL KQRHCHNFH KPCAGCCTG 
      VLEVLPLSA AYGLDDLYQ KCLQWITQH FVRVWPSRA 
      FASLPRELR EKCYQQHVV HMTADKVLE TIVSCDKVL 
      ATLPALRWA EPVTQLVLQ LSDACHLYH RQHFAAVLA 
      SPSFLALDA GLGWSVGQV EDQMVSAAG NLSVEQACR 
      SYARCCKMV DQSWSRPFN DLLFKIKVQ LEQCLVAQS 
      DRLIRSNAW LRLDSALRC RIKDLTPVA EPVSRLPRA 
      TPKRTKKSP LQSATKPHS QSPTISSSD SSRNSSPGM 
      NQAQSRLHN SGGSPSLRR SLLLAARAP QVPPSPSTH 
      RRSTLTQPT QASAAKSAP SKNIRNPPP KAATPTTTV 
      KPAQKKAPA RNAVSNPNL RKPNPTLSS KKPISRSSS 
      PLKVESRAS SRVTSPIKA DVRTPSPKK MHNGKVGSP 
      RKEVKESKE SKPVVPTMQ RSSTFLKDE PTVLGKVK
    CDS sequence
    • >TC010343 RA
      atggactcg ggtctggga agcgacgaa gaccgccga 
      ggtcgcacc aaggagcag aagcagctc cgcaaccag 
      cagctcctc agcgggtgc ttcatcgac gccgcgagc 
      ctctccgac gatgccgag gtcgaccag cggcgcacc 
      gacgagcga agacagggg gccctcatt ttccagtcg 
      tccattccg cagttttcc atgcttcag atggccgga 
      aacgaggcc gcgccgacg taccccgaa accagcacg 
      ccctacaac tccatcgtc cacctggag gaggacgcc 
      tccaggaag tcgccgctg gggttctac gtggacttg 
      aacgaggtg cccgacccg ccgaagacg ccgccgccg 
      gcgacagcc gccaagaag aacatcttc tccatggtc 
      atcgacttc gaaggcccc aagaaggac aagcccacc 
      aagctgagc tcctctctg gtctcctac aagaagaac 
      aggcagtcc aagcaactg actctcacc acgaggtac 
      aacgggaac agcagcctc tcctcgagt gtgagctcc 
      ataagttca gtggctggg cccagcaga caggtggag 
      gatgagaag gaggaagtg gagggtcag aaccaaatg 
      tcggcttcg tcgaagtcg tccagctcg agtgagaat 
      ttcccgatg cacaagagc gaggacgag ggacacgtg 
      gaggagcat aaacccgcc gaagacgac gtcgtggaa 
      aaaaaatgc gaagagact aacgctaaa aacgaagaa 
      attaaagaa gttgagact caggaacgg ccccagttc 
      gtcaaactc tccgactta gaatgtcaa aaaccaaga 
      ctggatctg aacttcact ccccggatg agcaaatct 
      atccctgag agttcatgg gtggaaagt ccacttttg 
      atgtctagg tctgccggt tatcgcagc gcaccacat 
      cctcttgaa cccgaatct ggagagttt tctgattcg 
      ggtagtgct actgatacc agcgtatca caacctagg 
      cgttcggtg cggcgttta ggaacagat ctgctccga 
      atgtttttg gaggaaatt ggaccagat gttaccgtc 
      gaagtagac gggcataaa ctgaaagca cacaaatgc 
      attctggcg tctaggtgc cagtatttc gccgcttta 
      ttgagcggg aattggttg gaagcatcc ggaaatatt 
      atctcactg caaggcttc tcgtatgaa tcagtttac 
      ttcgctctc tgtcacatc tacagtggg gccgcacac 
      gtgcccgac tcaatcagc ctcgtagaa ctcgcctca 
      ctggccgac atgttaggc ttagaaggc ctcaaagaa 
      gtagtcgag cacgcttta aaacagcgt cactgtcac 
      aactttcac aaaccatgt gccggctgc tgtaccgga 
      gtcctggaa gtccttccg ttatcagcc gcctacggc 
      ctggacgac ctctaccag aaatgcctc caatggatc 
      acccagcac ttcgtccga gtgtggcca agtcgtgct 
      tttgccagt ttgccgcgc gagttgcgc gaaaagtgc 
      taccagcaa cacgtcgtt catatgacg gccgacaag 
      gtgctagaa actatagtg agttgtgat aaggtcctg 
      gccacttta cctgccctg agatgggct gagcccgtc 
      actcagttg gtcttgcaa ctgtcggat gcttgtcat 
      ctgtaccat aggcagcat ttcgcggcg gttttagcg 
      tcgccgtcg tttctggct ttggatgct ggattggga 
      tggagtgtg ggacaggtc gaggaccag atggtgtct 
      gctgcaggg aatttgagt gtggaacag gcgtgcagg 
      agttatgcg aggtgttgc aagatggtt gatcagagc 
      tggagcagg ccgtttaat gatctgttg tttaaaatt 
      aaagtgcaa ttagaacag tgtttagta gcgcagtct 
      gataggctg attcgaagt aatgcatgg ttgaggctg 
      gattcggca ttgaggtgc cgcataaag gacttaacc 
      cccgtggcg gagcccgtg agtcgcttg ccccgggcg 
      accccaaaa cgaacgaaa aaatcgcct cttcaatca 
      gctacgaaa ccgcacagc cagagcccg acaataagc 
      tcgtccgac tcctcccgt aactcaagc cctggaatg 
      aaccaagcc cagtctcgt ctacacaat tccggcggc 
      agtccctcc ctccgcagg agtctcctt ctcgctgca 
      agggcgccc caggtgccc cccagcccg tccacccac 
      cggcgcagc actctgacc cagcccacg caagccagc 
      gccgccaaa tcggctccc tccaagaac atcaggaac 
      cctccgccc aaagctgcg acacctacc accaccgtc 
      aaaccggcg cagaaaaaa gccccagcg cgaaacgcc 
      gtctctaac cctaatctg cggaaaccg aatccgacg 
      ctttctagc aaaaaaccc atcagtagg tcgagttcg 
      cccttgaag gttgagagc agggcgagt tcgagggtc 
      acttcgccc ataaaagcc gacgttcgg acgccttcc 
      cctaagaaa atgcataac gggaaagtg gggtcgcct 
      aggaaggaa gtgaaggag agtaaggag agcaaaccg 
      gtggtacct acgatgcaa aggtcgagc actttcctg 
      aaggatgaa cctactgtt ttggggaag gttaagtaa
    mRNA sequence
    • >TC010343 RA
      ttcagagcg ttgtagaga attacttaa gtggttcaa 
      atatagaaa aaaaataaa gatttgaga cttccccat 
      aaaaatata ttggtttaa agatcactc tttgatctg 
      cgttttctt cagatgaac ctttcaaac aaacaaaaa 
      tgggttaac tgctttata ttttttgaa atcgcctaa 
      aaactttat caaacatta tcttcaaac aatacttat 
      gtcgtacac aacgtgatg tagaaaagt agcaataaa 
      gatagccgc caattggta aacatattg aaaaacacg 
      attctaaat tggtgaaag cgcaattta atccttttt 
      agattagtc ccggaaggt tatcttcta caataagtg 
      gcatcaatt aatttaaat taatggaaa atttcggtt 
      tgtgttatc tatgctaat tcaaatcca atcatcagg 
      agaaaatat ttcactaaa cgagttgtt gtttcaacc 
      aggagtgca gaaatttat gcagattcg agatttctt 
      attgaataa taaaagcga ccatttcaa agtaattta 
      ccgcgcttt attggggcc atgcgcatg cgcccttca 
      gtcaacccc aacccgaag acacatcga tctccagcc 
      gaactcagc cgacacttg acagaacct gttcattca 
      atttcacgc tcgcataaa acaaaatag tcaatagtc 
      ggagtcggt gtcatgttc cagtgttcc aaactccac 
      taaaatgcg cttttccgg ccattcctt cgttccgcc 
      tctggaagc gtgaaccca gaatcgatt tttgtgtga 
      gctatgtga agagccatg gactcgggt ctgggaagc 
      gacgaagac cgccgaggt cgcaccaag gagcagaag 
      cagctccgc aaccagcag ctcctcagc gggtgcttc 
      atcgacgcc gcgagcctc tccgacgat gccgaggtc 
      gaccagcgg cgcaccgac gagcgaaga cagggggcc 
      ctcattttc cagtcgtcc attccgcag ttttccatg 
      cttcagatg gccggaaac gaggccgcg ccgacgtac 
      cccgaaacc agcacgccc tacaactcc atcgtccac 
      ctggaggag gacgcctcc aggaagtcg ccgctgggg 
      ttctacgtg gacttgaac gaggtgccc gacccgccg 
      aagacgccg ccgccggcg acagccgcc aagaagaac 
      atcttctcc atggtcatc gacttcgaa ggccccaag 
      aaggacaag cccaccaag ctgagctcc tctctggtc 
      tcctacaag aagaacagg cagtccaag caactgact 
      ctcaccacg aggtacaac gggaacagc agcctctcc 
      tcgagtgtg agctccata agttcagtg gctgggccc 
      agcagacag gtggaggat gagaaggag gaagtggag 
      ggtcagaac caaatgtcg gcttcgtcg aagtcgtcc 
      agctcgagt gagaatttc ccgatgcac aagagcgag 
      gacgaggga cacgtggag gagcataaa cccgccgaa 
      gacgacgtc gtggaaaaa aaatgcgaa gagactaac 
      gctaaaaac gaagaaatt aaagaagtt gagactcag 
      gaacggccc cagttcgtc aaactctcc gacttagaa 
      tgtcaaaaa ccaagactg gatctgaac ttcactccc 
      cggatgagc aaatctatc cctgagagt tcatgggtg 
      gaaagtcca cttttgatg tctaggtct gccggttat 
      cgcagcgca ccacatcct cttgaaccc gaatctgga 
      gagttttct gattcgggt agtgctact gataccagc 
      gtatcacaa cctaggcgt tcggtgcgg cgtttagga 
      acagatctg ctccgaatg tttttggag gaaattgga 
      ccagatgtt accgtcgaa gtagacggg cataaactg 
      aaagcacac aaatgcatt ctggcgtct aggtgccag 
      tatttcgcc gctttattg agcgggaat tggttggaa 
      gcatccgga aatattatc tcactgcaa ggcttctcg 
      tatgaatca gtttacttc gctctctgt cacatctac 
      agtggggcc gcacacgtg cccgactca atcagcctc 
      gtagaactc gcctcactg gccgacatg ttaggctta 
      gaaggcctc aaagaagta gtcgagcac gctttaaaa 
      cagcgtcac tgtcacaac tttcacaaa ccatgtgcc 
      ggctgctgt accggagtc ctggaagtc cttccgtta 
      tcagccgcc tacggcctg gacgacctc taccagaaa 
      tgcctccaa tggatcacc cagcacttc gtccgagtg 
      tggccaagt cgtgctttt gccagtttg ccgcgcgag 
      ttgcgcgaa aagtgctac cagcaacac gtcgttcat 
      atgacggcc gacaaggtg ctagaaact atagtgagt 
      tgtgataag gtcctggcc actttacct gccctgaga 
      tgggctgag cccgtcact cagttggtc ttgcaactg 
      tcggatgct tgtcatctg taccatagg cagcatttc 
      gcggcggtt ttagcgtcg ccgtcgttt ctggctttg 
      gatgctgga ttgggatgg agtgtggga caggtcgag 
      gaccagatg gtgtctgct gcagggaat ttgagtgtg 
      gaacaggcg tgcaggagt tatgcgagg tgttgcaag 
      atggttgat cagagctgg agcaggccg tttaatgat 
      ctgttgttt aaaattaaa gtgcaatta gaacagtgt 
      ttagtagcg cagtctgat aggctgatt cgaagtaat 
      gcatggttg aggctggat tcggcattg aggtgccgc 
      ataaaggac ttaaccccc gtggcggag cccgtgagt 
      cgcttgccc cgggcgacc ccaaaacga acgaaaaaa 
      tcgcctctt caatcagct acgaaaccg cacagccag 
      agcccgaca ataagctcg tccgactcc tcccgtaac 
      tcaagccct ggaatgaac caagcccag tctcgtcta 
      cacaattcc ggcggcagt ccctccctc cgcaggagt 
      ctccttctc gctgcaagg gcgccccag gtgcccccc 
      agcccgtcc acccaccgg cgcagcact ctgacccag 
      cccacgcaa gccagcgcc gccaaatcg gctccctcc 
      aagaacatc aggaaccct ccgcccaaa gctgcgaca 
      cctaccacc accgtcaaa ccggcgcag aaaaaagcc 
      ccagcgcga aacgccgtc tctaaccct aatctgcgg 
      aaaccgaat ccgacgctt tctagcaaa aaacccatc 
      agtaggtcg agttcgccc ttgaaggtt gagagcagg 
      gcgagttcg agggtcact tcgcccata aaagccgac 
      gttcggacg ccttcccct aagaaaatg cataacggg 
      aaagtgggg tcgcctagg aaggaagtg aaggagagt 
      aaggagagc aaaccggtg gtacctacg atgcaaagg 
      tcgagcact ttcctgaag gatgaacct actgttttg 
      gggaaggtt aagtaaagg gatgtttcc agctagtcc 
      attcctagt caggcatat ttttgtttc gtttttgat 
      ctcgttatt gtattattt ttgtatgtt tgtaagaca 
      ttgtgatat atattacta tgcttcaca tgaatcaaa 
      ttttagtcg ttttaaatt attgtagct aaaagtact 
      aatttttct cagttgttt cgttgatac attccataa 
      atgtgataa tttaactga tgctaagca cgggggcac 
      tttatttct aacggaata ttctgtaaa aagtgtttg 
      gaggtgcca gctgtttat gctacccac attttattg 
      atttttgta cttttgtac gaaaaatag tcattctgc 
      tcttattct ctaaattaa attcaagac tgaatttat 
      tgaacataa caacttatg gttgtttta aagtacaaa 
      ttttgggta atcaaatta atcgacgtg ggtagtatt 
      ctgtgattt ttcgatatt ttaacgctt tttaaatta 
      attttaatc atgttcatg tgaagttcc tgatatttg 
      attatggag gagtaaata ttaggaggt gacacgtcc 
      tgatggtct aaattagat taatttaaa ccagagttc 
      tttattagg tattgcctg cgggttcct aaatactag 
      gatgcgaca cattaacct taaataacg atgtgagca 
      atagtaaaa aagttagag tgagatagt tttcaataa 
      gataacgac ataacaaga caaagtgta aagtacaaa 
      gttgtgcaa aattaaatt ttcttgaaa aaagtctac 
      gggaacatt ttgcctttt aaacttacg ttttttcta 
      tagcaaacg ttgaactat ttctttatc aactctttt 
      gagtagata aaatagcac tttattcaa ttaaattta 
      caaattcca actattaat ttgaaggat taaacttgg 
      aggtctgct atagaaaaa atatattat acaagacaa 
      taaagaccg tttatctgc ttgtgaaat aagtcgact 
      cgtaacttc gtcactgtt gaacttaat cttttcact 
      cataaataa aaaatcggt ggtggtaat agaccaaaa 
      aatctcttt atcgtcttg tagaattaa taactaatc 
      gcttttact gtccgaaaa cgatgagtt aagcaccag 
      ttttgttaa aatcagttg ttttcggac gaaatgaca 
      aaaaagttc agaaatgac aaaacacga tgtagaaaa 
      cctgaaata gttttattc tgaagctga aaacgacgc 
      gagttaagc accaatttt gcagaaatc agttaaatg 
      atataataa tagttgaat acaaaaata tttgttgct 
      ataaaaaac ttaaaaatg tggtaataa aaatattga 
      gatttataa gaatttcta aaaataacg tgttcaagt 
      ttttttttt aatgacaag atgtgaatt ttatccgtt 
      attttaaat tcgccatta attatttca cttccctca 
      tcagagcta acgatggag agggaaagg gagtggagt 
      agtttataa gtttattct gttcatgtt aaatcttgc 
      cctaattga ttgactttt accaatttt gtctctttc 
      tttgcttca gcaatttct caaaaaata tatttaagt 
      tatcagctt ttgatattt tgcaccacg atctgcact 
      agttaaatt aataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY