Status
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Gene information
General information
TC010334 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24936734..24938121
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010334
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04816

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    low validity due to technical reasons - should be repeated: cuticle phenotype not scorable,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010334 RA
      MTAKNIQDF EVVPFYFSS DSTDPHDLF IKEHKVRKF 
      DIKKPPGKT LFVLNVPPY VTVEALKDV FASAGKVQS 
      VTFQCDDSD DTNELFGFK TAFVVFTKR PSLLRALKL 
      ERLTLPNPH NILLGMSKW IHEYNSTVY NHDKLNEEV 
      NIYIKQHEK EEEEKKLAE KEVDDEGWT VVTKKGRNP 
      GLSRKESVE NKLIQKNKI KSKKKELKN FYTFQLKEQ 
      KMNNIIALR KSFQESKEK VNLMKKMRN FKPF
    CDS sequence
    • >TC010334 RA
      atgacggct aaaaatata caggacttt gaagtggta 
      cccttttac ttctcaagt gatagcacc gacccccat 
      gatttattt atcaaagaa cacaaagtc cgaaaattc 
      gacataaaa aagcccccc ggaaagaca ctttttgtg 
      ctcaacgtg cccccctat gtcactgtc gaagccctg 
      aaggacgtg ttcgcctca gctgggaaa gtacaatcc 
      gttacgttc caatgtgat gatagtgat gatacaaac 
      gaactgttt gggttcaaa acagctttt gtggttttt 
      acgaaacgt ccaagtctc ttaagagca ttgaaattg 
      gagcgcttg acgttgccc aacccgcat aacatttta 
      ttaggcatg agtaagtgg attcacgag tataattca 
      acggtttac aatcacgat aaactgaac gaagaagtg 
      aacatttat atcaaacaa cacgaaaag gaggaagaa 
      gaaaagaaa ttggctgag aaggaagtg gatgatgag 
      ggctggact gtggtgacg aaaaaaggg cgaaatccg 
      ggcctgtct cgcaaggaa agtgtcgaa aataaacta 
      atacagaag aacaaaatc aaatcaaag aagaaagaa 
      ttgaagaat ttttacaca ttccaactg aaagaacag 
      aagatgaat aacataatc gccctgcgg aagagcttc 
      caagaatca aaggaaaag gtgaatttg atgaaaaaa 
      atgcgtaat tttaaaccc ttttaa
    mRNA sequence
    • >TC010334 RA
      agacaattt ttgaataat tcaccattt cacctgaaa 
      cacgcccca aacataacc tcaaaacgt caacacgtg 
      caaaatgac ggctaaaaa tatacagga ctttgaagt 
      ggtaccctt ttacttctc aagtgatag caccgaccc 
      ccatgattt atttatcaa agaacacaa agtccgaaa 
      attcgacat aaaaaagcc ccccggaaa gacactttt 
      tgtgctcaa cgtgccccc ctatgtcac tgtcgaagc 
      cctgaagga cgtgttcgc ctcagctgg gaaagtaca 
      atccgttac gttccaatg tgatgatag tgatgatac 
      aaacgaact gtttgggtt caaaacagc ttttgtggt 
      ttttacgaa acgtccaag tctcttaag agcattgaa 
      attggagcg cttgacgtt gcccaaccc gcataacat 
      tttattagg catgagtaa gtggattca cgagtataa 
      ttcaacggt ttacaatca cgataaact gaacgaaga 
      agtgaacat ttatatcaa acaacacga aaaggagga 
      agaagaaaa gaaattggc tgagaagga agtggatga 
      tgagggctg gactgtggt gacgaaaaa agggcgaaa 
      tccgggcct gtctcgcaa ggaaagtgt cgaaaataa 
      actaataca gaagaacaa aatcaaatc aaagaagaa 
      agaattgaa gaattttta cacattcca actgaaaga 
      acagaagat gaataacat aatcgccct gcggaagag 
      cttccaaga atcaaagga aaaggtgaa tttgatgaa 
      aaaaatgcg taattttaa accctttta attaaaaaa 
      ataaaactt tatttcgac tctttgtgc gtatttatg 
      ataatactc aataattta ttgaaagga agttgttgc 
      aactggctc atgagatca tctattttc ccatagaca 
      catttagtc gcaagggca acttgcaca gcttgtgca 
      aatctgtct aacgcgatt tgtaaattg ggttgtttg 
      ccccctttg ttgctcgac aataaaatg tgtctcagg 
      gctacggtc aaatgttgg gcgctgatc gctttaggg 
      ggctgtgtt tgcggtatt tcaaggatg ctgttaagc 
      agcgctaag tgtaaatga caatacaag tccgagtct 
      tggtcgcat aagggggcc aatgtgcac catctgatg 
      agccccgcg attgcagtt gtggcctcc atggcgata 
      gcccccggg ggcagagcg gcttgtttc tgctgggcg 
      gctatgcac aaacaggca ttttgcgaa atctgaaac 
      agagttaaa cagacatta aataatata cgttattag 
      tgtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY