Status
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Gene information
General information
TC010330 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24992949..24998600
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010330
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04814

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010330 RA
      MPFDINNKV ALISGAASG IGLTIAKEL LRNGLRGVT 
      IADVNKELG EKALQEIEN EFGSGRAIF VLTDVSSMK 
      SFEDAFQKT INTFKNIDI LVNGAGVLN DSIWQQEIA 
      INVNGTVHG ILLALENYI PKYKTDSEG VIVNFSSIA 
      GVAAFPAFP IYTGTKFAI FGMTKAFGD EAHYERTKV 
      RIMAICPGP TDTPLLRDI SGRNLGAPY EKLLQELLK 
      DTRGQKPEA VARGLVQIV RNAPSGGAW FDIKNKVAL 
      ISGAASGIS LAIAKAFLR NGLRGVTIA DINTELGEK 
      VLQEIINEF GKNKAIYIL TDVTCKNSY EAAFKKTIE 
      TFQNLDIVV NGAGIVNEL DWQQEVDIN LNGTLHGTI 
      LALENYIPK YKSGSEGVI VNISSAAIL DCFPPYPIY 
      TGTKFAVMG ISKSFGAEV HYKRTKVRV LTACPGVTE 
      TPFGIQASE KCLSEPYRK LKDEYFSGI YEQKPESVA 
      EGVTEIVKN APTGTAWIM VEDKKPEEL VWPSEGKNF 
      APKKKK
    CDS sequence
    • >TC010330 RA
      atgcccttc gatattaac aacaaagtg gccctaatt 
      tctggagct gcatcaggt ataggttta acaattgca 
      aaagaattg ttacgaaat ggattaagg ggcgttaca 
      atagctgac gtaaacaaa gaactggga gaaaaagct 
      ctacaggaa atagaaaat gaattcggg tcgggtaga 
      gccattttt gtgctcacc gatgtatca agcatgaag 
      agttttgaa gatgcattt cagaaaact ataaacacg 
      ttcaagaat attgacatt ttagtcaac ggtgcaggc 
      gttttaaac gattcgatt tggcaacaa gagatagca 
      ataaacgtc aacggaaca gttcatggt attctcctg 
      gctttggaa aactacatt ccaaaatac aaaactgat 
      tctgaaggt gttatcgta aacttttca tcaatcgcc 
      ggtgttgcg gcttttcca gcatttcca atttacact 
      ggaacgaaa ttcgcaatt ttcggcatg actaaagca 
      tttggagat gaggcacat tatgaaagg accaaagtc 
      aggataatg gctatttgt ccaggacct acagatacg 
      ccccttctt agggatatt tcaggtcgc aatttaggg 
      gcaccttac gaaaagtta ctacaagaa ttacttaag 
      gacacgcgt ggtcaaaag ccagaagca gtcgctaga 
      ggtttggtt caaattgtg agaaatgct ccgtctggt 
      ggtgcatgg tttgacata aaaaacaaa gtggcttta 
      atttctgga gcagcatca ggaatatct ttagccatt 
      gctaaagca tttttacgc aatggatta aggggggtt 
      acaattgct gacataaac accgaactt ggcgaaaag 
      gttttacaa gagattata aatgagttt ggaaaaaat 
      aaagctatc tatattctc accgatgta acttgtaag 
      aatagctat gaagcggca tttaaaaaa accatcgag 
      acgtttcaa aacttagac attgtggtc aatggtgca 
      ggaattgta aacgaatta gattggcaa caagaagtt 
      gacataaat ttgaatggt actttgcac ggtacaatt 
      ctcgctttg gaaaactac atcccaaaa tacaaatct 
      ggttccgaa ggtgtaatt gtcaatatt tcgtcagcg 
      gctatttta gactgtttc cctccatat ccaatctac 
      accggtaca aaatttgca gtgatggga ataagtaaa 
      tcatttggt gctgaagta cattacaaa aggaccaaa 
      gtcagagtt ttgactgca tgtccagga gttacagaa 
      acaccattt ggtatacaa gcatcagaa aaatgtttg 
      agtgaacct tatcggaaa ttaaaggat gaatatttt 
      tccggaata tatgagcaa aaaccggaa agtgtggct 
      gaaggtgtg actgaaatt gtgaaaaac gccccaact 
      ggtactgca tggattatg gtggaggat aaaaaacca 
      gaggagttg gtgtggcca tctgaagga aagaatttt 
      gctcccaag aaaaagaag tga
    mRNA sequence
    • >TC010330 RA
      tttttacac ttgcaaata gtttttttt gaaaatata 
      aatgtcatt atcataaat tattattgt tatgcgcta 
      ctatctact aaaaatgaa aataaatct ttacaccca 
      ctatgatta caataatac gttatcgtt aactggtat 
      tatatttta aaagtaacg tcataacaa aattattaa 
      tttcctttt tatcatcac caatcttaa cttactatt 
      gacctaatt atcaatcaa taatacata aaccgtaca 
      atttagtgc taaataatt cgtcgtttc aaagttaat 
      agaaattca attttgctt ttgaattat cggtaaaaa 
      cacaacaat gcccttcga tattaacaa caaagtggc 
      cctaatttc tggagctgc atcaggtat aggtttaac 
      aattgcaaa agaattgtt acgaaatgg attaagggg 
      cgttacaat agctgacgt aaacaaaga actgggaga 
      aaaagctct acaggaaat agaaaatga attcgggtc 
      gggtagagc catttttgt gctcaccga tgtatcaag 
      catgaagag ttttgaaga tgcatttca gaaaactat 
      aaacacgtt caagaatat tgacatttt agtcaacgg 
      tgcaggcgt tttaaacga ttcgatttg gcaacaaga 
      gatagcaat aaacgtcaa cggaacagt tcatggtat 
      tctcctggc tttggaaaa ctacattcc aaaatacaa 
      aactgattc tgaaggtgt tatcgtaaa cttttcatc 
      aatcgccgg tgttgcggc ttttccagc atttccaat 
      ttacactgg aacgaaatt cgcaatttt cggcatgac 
      taaagcatt tggagatga ggcacatta tgaaaggac 
      caaagtcag gataatggc tatttgtcc aggacctac 
      agatacgcc ccttcttag ggatatttc aggtcgcaa 
      tttaggggc accttacga aaagttact acaagaatt 
      acttaagga cacgcgtgg tcaaaagcc agaagcagt 
      cgctagagg tttggttca aattgtgag aaatgctcc 
      gtctggtgg tgcatggtt tgacataaa aaacaaagt 
      ggctttaat ttctggagc agcatcagg aatatcttt 
      agccattgc taaagcatt tttacgcaa tggattaag 
      gggggttac aattgctga cataaacac cgaacttgg 
      cgaaaaggt tttacaaga gattataaa tgagtttgg 
      aaaaaataa agctatcta tattctcac cgatgtaac 
      ttgtaagaa tagctatga agcggcatt taaaaaaac 
      catcgagac gtttcaaaa cttagacat tgtggtcaa 
      tggtgcagg aattgtaaa cgaattaga ttggcaaca 
      agaagttga cataaattt gaatggtac tttgcacgg 
      tacaattct cgctttgga aaactacat cccaaaata 
      caaatctgg ttccgaagg tgtaattgt caatatttc 
      gtcagcggc tattttaga ctgtttccc tccatatcc 
      aatctacac cggtacaaa atttgcagt gatgggaat 
      aagtaaatc atttggtgc tgaagtaca ttacaaaag 
      gaccaaagt cagagtttt gactgcatg tccaggagt 
      tacagaaac accatttgg tatacaagc atcagaaaa 
      atgtttgag tgaacctta tcggaaatt aaaggatga 
      atatttttc cggaatata tgagcaaaa accggaaag 
      tgtggctga aggtgtgac tgaaattgt gaaaaacgc 
      cccaactgg tactgcatg gattatggt ggaggataa 
      aaaaccaga ggagttggt gtggccatc tgaaggaaa 
      gaattttgc tcccaagaa aaagaagtg acaaactaa 
      actgttgtc ttccgcttg atttaaaaa attaaatca 
      gcttcagaa tacattgtt taaatcgtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY