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Gene information
General information
TC010324 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25262115..25285080
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010324
    iBeetle screen iB_04810

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010324 RA
      MRGRSGRRS RSRDRRRSR SRDRGYGGG GGRRNTAGA 
      NLRKPRWDL SRLEPFKKD FYVPHPNVT NRPSYEVEE 
      WRREKEITL KGKCIPDLV FTFEEAGFP DYVMSEIRK 
      MGFKHPTPI QSQGWPIAL SGRDMVGIA STGSGKTLS 
      YILPAIVHI NHQPRLLRG DGPIALVLA PTRELAQQI 
      QQVATDFGR SSKIRNTCV FGGAPKGPQ ANDLMDGVE 
      IVIATPGRL IDFLESNRT NLRRCTYLV LDEADRMLD 
      MGFEPQIRK IIEQIRPDR QTLMWSATW PKEVQALAA 
      EFLKDYIQI NVGSLQLSA NHNILQIID VCQEYEKET 
      KLSTLLKEI MAEKENKTI IFIETKKRV DEITRKMKR 
      DGWPAVCIH GDKSQQERD WVLQDFRTG KAPILVATD 
      VAARGLDVE DVKFVINFD YPSNSEDYV HRIGRTGRS 
      QRTGTAYTF FTPANANKA ADLVSVLKE AKQVINPKL 
      QEMADNRNW SSNGSSGRG RSRWGRGRG HQPSHRSVS 
      RSRSRSPRR GRRDRSRSR DRNRRKKSR STSRDRSPP 
      RNSKSAPSQ QPPPQPLMS YQNIPPPNT FTYSLSNGS 
      MFFNAPPPP PPTQGVTGL YYNYTAPPP APR
    CDS sequence
    • >TC010324 RA
      atgagggga cgttctgga cgaagatca aggagtcga 
      gaccgccgt cgatcgcga agccgcgac cggggttac 
      ggtggcggt ggtggccgt cgaaacacc gccggtgct 
      aacttacga aaaccccgt tgggattta agccgtctt 
      gagccgttc aaaaaagac ttctatgta ccacatcct 
      aacgtaaca aaccgtcca agctacgaa gtggaagaa 
      tggcgaaga gagaaagaa attaccctc aaaggaaaa 
      tgcatcccc gatttagtg ttcacattc gaagaagct 
      ggttttcca gactacgtc atgtccgaa attcgcaaa 
      atgggattc aaacatccg acacctata cagtcgcaa 
      ggatggcct attgcttta tcgggacgt gacatggtt 
      ggaatcgct tcgacaggg tctggaaaa acgctttcg 
      tatattctg ccagcgatt gtgcacatt aaccatcaa 
      cccaggtta ttacgaggt gatggaccc attgcgttg 
      gtgctggcg cccacgagg gagctggca cagcagata 
      caacaggtc gcaactgat ttcggccgt tcatcaaaa 
      attcgcaac acttgcgtt tttggaggc gcccccaaa 
      ggacctcaa gctaacgat ttgatggat ggtgtagaa 
      atagttata gcaaccccg ggcagattg atcgacttt 
      ttagaaagt aatagaacg aaccttcga cgttgcacg 
      tatttagtt ttagacgag gcagatcgg atgcttgat 
      atgggtttt gaaccccaa attcgtaaa ataatcgaa 
      cagattaga ccagataga cagaccctg atgtggtca 
      gcgacttgg cctaaagaa gttcaagcc ctagcggct 
      gagtttcta aaagactac attcagata aatgtaggt 
      tctttgcag ttgtctgca aatcataac atacttcag 
      attattgat gtgtgtcag gaatatgag aaagagacg 
      aaattgagt acgttactt aaagaaatc atggccgag 
      aaggaaaat aagacgatc atatttatt gaaactaag 
      aagagagtt gatgagatt accagaaaa atgaaacgt 
      gacggatgg ccagcggtt tgcatacat ggagataag 
      tcgcaacag gagagagat tgggtttta caagacttc 
      aggactggt aaggcccct attctcgtg gcgactgac 
      gtggcggct cgaggttta gatgtagaa gacgtaaaa 
      tttgtaatc aattttgac tacccgtcc aattccgaa 
      gactacgtg caccgtatt ggtcgcacg gggcgttcg 
      caacgcacc ggaacagcc tatacattt ttcacacca 
      gcaaacgcc aacaaagcg gccgatttg gtgtccgtt 
      ctcaaagaa gccaaacaa gttatcaat ccgaaactc 
      caagaaatg gccgacaat cgcaactgg agctcgaac 
      ggcagttca ggtcgaggg cgcagtcgt tggggcaga 
      ggccgaggc caccagccc tcgcacagg tctgtctcc 
      cgctccagg tcgcggtct cctcgcaga ggaagacgc 
      gacagaagt cgatcgaga gacagaaat aggagaaag 
      aagtcgcgt agcacaagc agggaccgc agtcctccc 
      cgtaatagc aaatcagca ccctctcaa cagccccct 
      ccacaaccc ttgatgtcg tatcaaaat atcccgcca 
      ccgaataca ttcacatac tctttgtcc aatggatcg 
      atgttcttt aatgcaccg cccccaccg ccacccaca 
      cagggtgtc acagggttg tattacaac tacacagcg 
      ccgcctcca gcacctcgt tga
    mRNA sequence
    • >TC010324 RA
      aagcgcact tgtgattcg ttgtttttt attgacgcc 
      atctttgct gtttaaaga tgtctgtta ggcaatttg 
      ttgttgtat aaaattgtt gtttatgaa aatagtgaa 
      taaatttga atcgtttta cgataagtg ttaagtaat 
      tcaaggaca tgaggggac gttctggac gaagatcaa 
      ggagtcgag accgccgtc gatcgcgaa gccgcgacc 
      ggggttacg gtggcggtg gtggccgtc gaaacaccg 
      ccggtgcta acttacgaa aaccccgtt gggatttaa 
      gccgtcttg agccgttca aaaaagact tctatgtac 
      cacatccta acgtaacaa accgtccaa gctacgaag 
      tggaagaat ggcgaagag agaaagaaa ttaccctca 
      aaggaaaat gcatccccg atttagtgt tcacattcg 
      aagaagctg gttttccag actacgtca tgtccgaaa 
      ttcgcaaaa tgggattca aacatccga cacctatac 
      agtcgcaag gatggccta ttgctttat cgggacgtg 
      acatggttg gaatcgctt cgacagggt ctggaaaaa 
      cgctttcgt atattctgc cagcgattg tgcacatta 
      accatcaac ccaggttat tacgaggtg atggaccca 
      ttgcgttgg tgctggcgc ccacgaggg agctggcac 
      agcagatac aacaggtcg caactgatt tcggccgtt 
      catcaaaaa ttcgcaaca cttgcgttt ttggaggcg 
      cccccaaag gacctcaag ctaacgatt tgatggatg 
      gtgtagaaa tagttatag caaccccgg gcagattga 
      tcgactttt tagaaagta atagaacga accttcgac 
      gttgcacgt atttagttt tagacgagg cagatcgga 
      tgcttgata tgggttttg aaccccaaa ttcgtaaaa 
      taatcgaac agattagac cagatagac agaccctga 
      tgtggtcag cgacttggc ctaaagaag ttcaagccc 
      tagcggctg agtttctaa aagactaca ttcagataa 
      atgtaggtt ctttgcagt tgtctgcaa atcataaca 
      tacttcaga ttattgatg tgtgtcagg aatatgaga 
      aagagacga aattgagta cgttactta aagaaatca 
      tggccgaga aggaaaata agacgatca tatttattg 
      aaactaaga agagagttg atgagatta ccagaaaaa 
      tgaaacgtg acggatggc cagcggttt gcatacatg 
      gagataagt cgcaacagg agagagatt gggttttac 
      aagacttca ggactggta aggccccta ttctcgtgg 
      cgactgacg tggcggctc gaggtttag atgtagaag 
      acgtaaaat ttgtaatca attttgact acccgtcca 
      attccgaag actacgtgc accgtattg gtcgcacgg 
      ggcgttcgc aacgcaccg gaacagcct atacatttt 
      tcacaccag caaacgcca acaaagcgg ccgatttgg 
      tgtccgttc tcaaagaag ccaaacaag ttatcaatc 
      cgaaactcc aagaaatgg ccgacaatc gcaactgga 
      gctcgaacg gcagttcag gtcgagggc gcagtcgtt 
      ggggcagag gccgaggcc accagccct cgcacaggt 
      ctgtctccc gctccaggt cgcggtctc ctcgcagag 
      gaagacgcg acagaagtc gatcgagag acagaaata 
      ggagaaaga agtcgcgta gcacaagca gggaccgca 
      gtcctcccc gtaatagca aatcagcac cctctcaac 
      agccccctc cacaaccct tgatgtcgt atcaaaata 
      tcccgccac cgaatacat tcacatact ctttgtcca 
      atggatcga tgttcttta atgcaccgc ccccaccgc 
      cacccacac agggtgtca cagggttgt attacaact 
      acacagcgc cgcctccag cacctcgtt gatgtgagg 
      gttggttta ctgcggtac ctgacgatg gtgtggaaa 
      ttgtcctct tggttaaat aatctcggt ttttgtatt 
      atgtaaata ttacagata tttatttta acttgacta 
      ggttagatg gggtgatta ttaaaaatt gtgttcatc 
      g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY