Status
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Gene information
General information
TC010323 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25290024..25305431
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010323
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04809

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010323 RA
      MSKKFQKDV NKQVSSDCV KNTSNVSKS DEEIALELT 
      VGSANVKRW HLLKASFSC ETNENFTTT LLDLAQEYL 
      NRNPCKEEE EDGSSKKGD KGQISRNKR KKTETKKEK 
      NQANLITVT QVTPTSTTT KTKQPLLHA DIITQAVCG 
      KTDTANDAN LHNKKETVC KPSEDTAEK EEKTKKKKS 
      LTNLDKPIK KEKSTDTKK KLDHDIRKA PELGLQNDI 
      KLEPQKPEP ELNETVNRV ALKIKLCYN CNSQHVQDQ 
      CPLNYPNFV ISDLIDLPS WEEKLQSLQ TNNSELIKS 
      EKEDDANQF IFAYVSLPS FLQLMETNT EHGLGVWAK 
      MDIEEFTQF GPLVGKVVK EVDISEDSN MRDIWEIFV 
      DSKNVYFST ENLETSNWM RFVRPAPTR DDRNITVVA 
      KDSSLYFVS IKAIKNGEE LLYWQDSSI PSKKKMEKN 
      SCGGCNMTF AHPLYYRTH CSVFHDIRF SLTIRKYHC 
      KVCGAAVLG KENIMKHAA ELHNGQGAY QCQFCKKFF 
      LRLNYLEMH RNYGCSANP HRARPLCDF CGRKFCQPQ 
      KLKVHIKRM HSDFSEVLK EFQCKNCLK ILGSRAALQ 
      RHLKEVHQK QMDGACSCS RCGKHFQNK SNLKIHMLT 
      HSGIKPFKC VVKSCNAAF TTKQCLQFH YKKVHSFTE 
      DAMPKIERS IDYTFEAYS GLEDQEDKG KNEMNTQQS 
      DLNATPLEE ESDGDETSM DSKNVDDPS LPNSPMQPP 
      DDTSPDPNP RTSPTLESY NLNTVNATM KILSKGSKK 
      WIGEPPLQP LKPEIYQIE HQVTETQYD RPKSNLTDF 
      TRHEASNAS LLVEAALDS VCSEPNIDI DVSTTPNCT 
      DALVNNLYT LAPSDTLPD VTTYSETVC NINEPRDIN 
      LISPSVNDH ISVTDELND ELRHGQNIG MDYSGFHQE 
      DFSPGNSPE MHPRANFVR SYINTPNFG PKNLSPVPV 
      PSPPRYDFG HSVNTDHLS SDDSNGMAA QNLSLHSNK 
      HDIQLDLSI YKAPYNLQN RKEFRVKFD DLDAKSKYS 
      LIEPGKSYV EEENNALDG EQVIDQNEL SGNIVVDLK 
      EKEDEHERN KYPDDLSTD IRTNKFDID LDLRLKSYD 
      MEGENLRTR GLYESNYEK NYEIIDNIE NRKPYDPAL 
      DGDFRTDRN FEPLVLNSS ELQGLDMSA RSFHNYSNI 
      NRYHHLYPE VDRVDLRLN YSPPAPPYT HADILRVVS 
      LDLTPPGRH SVDLSLRTH PLHQIANSR LLSEHAPHR 
      LLTSDLSGR ILSDHTTNR ILSNNDQLG SNHLLGADQ 
      RLLGEESRI ISEQSRLIG DGRILAPTT PGGAVSPIP 
      FSGYSVSQS PYHPTPLAP RPHVTSPTP TPYHHYSTY 
      Y
    CDS sequence
    • >TC010323 RA
      atgtccaaa aagttccaa aaagatgta aacaagcaa 
      gtgtcaagt gattgtgtc aagaataca tcaaacgtg 
      tcaaaaagt gacgaggaa atcgcacta gagctaacg 
      gttggttct gcaaatgtc aaacgatgg catcttctc 
      aaggcttcg ttttcctgt gaaactaac gaaaacttt 
      accaccact ttgctggat ttggcacag gagtactta 
      aatcgcaat ccctgtaaa gaagaagag gaagatgga 
      agttccaag aaaggagat aagggacaa attagtaga 
      aataaacga aaaaagact gagacaaaa aaggagaag 
      aatcaagca aatttaatt actgtcaca caagtaaca 
      cctacttct acaaccaca aaaaccaaa caacctctt 
      cttcacgct gacatcata actcaagca gtttgtggt 
      aaaactgat acagcaaac gatgcaaac ttacacaac 
      aaaaaagag acagtttgt aagcctagt gaggataca 
      gccgaaaaa gaggaaaaa acaaaaaag aagaaatct 
      ttaaccaac ctcgacaaa ccaataaaa aaggaaaaa 
      tcaaccgac acaaagaag aaacttgac catgacatt 
      agaaaagca ccagaacta ggtttacaa aacgacatt 
      aaactcgaa ccccaaaaa cctgaaccc gaacttaac 
      gaaaccgtt aacagagtg gccctaaaa ataaaattg 
      tgctacaac tgcaacagc caacacgtg caggatcag 
      tgtcctttg aactacccc aactttgtc atctccgac 
      ttgattgat ttgccaagt tgggaggaa aaactccaa 
      agtttgcaa acaaataac tcagaattg atcaaatct 
      gagaaggaa gatgatgca aaccaattt atttttgcg 
      tacgtttcg ttgcccagt tttttacag cttatggaa 
      acgaacact gaacatggg ctgggagtg tgggcaaaa 
      atggacatc gaggagttc actcagttt ggccctctt 
      gtggggaaa gtggtcaag gaggtggat atttcggaa 
      gattcgaac atgagagat atttgggag atttttgtt 
      gatagcaag aatgtttat tttagtacc gaaaatttg 
      gaaacgtcg aattggatg cggtttgtt cgaccggct 
      ccaaccaga gacgataga aacattact gtggtggca 
      aaggacagt tctctctac tttgttagt attaaagcg 
      atcaaaaac ggggaagag ttgctgtac tggcaggat 
      tccagcatt ccgagcaag aaaaaaatg gagaaaaat 
      tcttgtggt ggttgtaat atgacgttt gctcatccg 
      ctctactac cgaacacat tgctcagta ttccacgac 
      attcgtttt agtcttact attaggaaa taccattgt 
      aaagtttgt ggggctgca gtgttgggt aaagaaaat 
      attatgaaa catgcagct gagttgcat aatggacag 
      ggggcctat cagtgccag ttttgtaaa aagttcttt 
      ttaagactg aattatcta gagatgcat cgtaattat 
      ggctgttct gctaatcca caccgagct aggcctttg 
      tgtgatttc tgtgggcga aaattttgt caaccccaa 
      aagttgaag gttcatatt aaaagaatg cacagtgat 
      ttttccgaa gttttgaag gaattccag tgcaaaaac 
      tgtctgaaa attctcggc tcgcgagca gctctacaa 
      aggcatttg aaggaagtt caccagaaa caaatggac 
      ggtgcttgt tcgtgttct cgttgtggc aaacatttc 
      caaaataaa tcaaattta aaaattcac atgttgact 
      cacagtggg attaaaccc ttcaagtgt gttgtaaag 
      agttgtaat gcggcgttt acaacgaaa cagtgtcta 
      cagttccac tataagaag gttcacagt tttactgag 
      gacgcgatg cctaagatt gaacgaagc attgactac 
      acttttgag gcctacagt ggattagag gaccaggag 
      gataaaggc aaaaatgag atgaatacg caacaaagt 
      gacttgaat gcgacacct cttgaggag gaaagtgac 
      ggggatgag accagtatg gacagcaaa aacgtggac 
      gacccatcc ttacccaac tccccgatg caaccaccc 
      gacgacacc tcccccgac cccaaccct cgaacctcc 
      ccaacgctg gaatcctac aacttaaac accgtaaac 
      gcaaccatg aaaatcctc agcaaaggt tcaaaaaaa 
      tggattggc gaaccacct ctccaaccc ctcaaaccc 
      gagatctac caaatcgag caccaagtc accgaaacc 
      caatacgac cgcccaaaa tcaaaccta accgacttc 
      acacgccac gaagcctca aacgccagt ctcctggtg 
      gaagcagcc ctggactcc gtctgcagc gaaccgaac 
      atagacatc gacgtgagc acaacaccg aattgcacc 
      gacgccttg gtcaacaat ttatacacg ttggctcct 
      tcggacacc cttccggac gtcaccacg tacagcgaa 
      acggtctgt aacataaac gaaccgcgc gatatcaat 
      ttaatttca ccgtcggtc aacgaccac atttccgta 
      acggatgag ttgaatgat gagttacga cacggccag 
      aacatcggc atggattat tccgggttc caccaggag 
      gacttcagt ccggggaac agcccggaa atgcacccg 
      cgcgccaat ttcgtccgg agttacatc aatacgccc 
      aactttggg cccaagaat ttatcaccg gttccggtg 
      cccagtcca ccacgttac gacttcggt cattccgtc 
      aacaccgat catctctca tcggatgat agcaacgga 
      atggccgcg caaaatcta agcctacat tccaataag 
      cacgacata cagttggat ttgtcgatt tacaaggcg 
      ccctataat ttgcaaaat cgcaaagag tttagggtg 
      aaattcgac gatttggac gcaaagtcc aagtatagt 
      ttaatcgag ccgggcaaa agttacgtg gaggaggaa 
      aataacgca ctggatggg gaacaagtg attgaccag 
      aacgaactc tccggcaat attgttgtg gatttgaag 
      gagaaagag gacgaacat gagcgtaat aaatacccc 
      gatgatctc tcgactgat attaggact aataagttt 
      gatattgat ctcgattta aggctgaaa agttacgac 
      atggagggc gaaaatttg cgaacgcgc gggttgtat 
      gaatcaaac tacgaaaaa aactacgaa attatcgac 
      aatattgag aatcgcaag ccttacgac ccagcgctt 
      gacggtgat ttccgcacc gatcgtaac tttgagcct 
      ctagtgctc aactcctcc gagctacaa ggcctggat 
      atgtcggct cggagcttc cacaactat tccaacatc 
      aaccgttac caccacctc taccccgag gttgaccgg 
      gttgatcta cggttgaat tacagtccc ccagctccg 
      ccttacacc cacgccgat atcctccgt gtggtttcg 
      ttagatttg acacctcca ggacgtcat agcgtcgat 
      ttgagtcta cggacgcat ccgttgcac cagatcgcc 
      aattcgagg ttgctttca gagcacgct ccgcaccgt 
      ctgttgacg tccgatttg agtgggagg attttgtcg 
      gatcatacg actaatcgc attttgtcg aataatgat 
      cagttgggg agtaaccat ttgttgggg gctgatcag 
      aggttgttg ggggaggag tcgcggatt atttccgag 
      caaagtcgg ctgattggg gatgggagg attttggcg 
      ccgacgacg ccaggtggg gccgtgtcg ccgattccg 
      ttcagtggg tattcggtg tcgcagagt ccgtatcat 
      ccgacaccg ttggcacct aggccgcat gttacgtca 
      ccgacacct acaccgtac catcattac tccacttat 
      tattga
    mRNA sequence
    • >TC010323 RA
      gaataattg tagactcgc caaaccaat tagataaaa 
      taaacttgc aactttcac gcatttgtc cgtgaagaa 
      agaggggaa cattgtcga tttcaagtt catgagcat 
      tttattgat cataaattt caacttggc gccatgtga 
      cgtcacgaa gtgtccttg gcttaaaga ggatagaac 
      tcttgatgg tctatcctc tttatggga atttacgca 
      atgtccaaa aagttccaa aaagatgta aacaagcaa 
      gtgtcaagt gattgtgtc aagaataca tcaaacgtg 
      tcaaaaagt gacgaggaa atcgcacta gagctaacg 
      gttggttct gcaaatgtc aaacgatgg catcttctc 
      aaggcttcg ttttcctgt gaaactaac gaaaacttt 
      accaccact ttgctggat ttggcacag gagtactta 
      aatcgcaat ccctgtaaa gaagaagag gaagatgga 
      agttccaag aaaggagat aagggacaa attagtaga 
      aataaacga aaaaagact gagacaaaa aaggagaag 
      aatcaagca aatttaatt actgtcaca caagtaaca 
      cctacttct acaaccaca aaaaccaaa caacctctt 
      cttcacgct gacatcata actcaagca gtttgtggt 
      aaaactgat acagcaaac gatgcaaac ttacacaac 
      aaaaaagag acagtttgt aagcctagt gaggataca 
      gccgaaaaa gaggaaaaa acaaaaaag aagaaatct 
      ttaaccaac ctcgacaaa ccaataaaa aaggaaaaa 
      tcaaccgac acaaagaag aaacttgac catgacatt 
      agaaaagca ccagaacta ggtttacaa aacgacatt 
      aaactcgaa ccccaaaaa cctgaaccc gaacttaac 
      gaaaccgtt aacagagtg gccctaaaa ataaaattg 
      tgctacaac tgcaacagc caacacgtg caggatcag 
      tgtcctttg aactacccc aactttgtc atctccgac 
      ttgattgat ttgccaagt tgggaggaa aaactccaa 
      agtttgcaa acaaataac tcagaattg atcaaatct 
      gagaaggaa gatgatgca aaccaattt atttttgcg 
      tacgtttcg ttgcccagt tttttacag cttatggaa 
      acgaacact gaacatggg ctgggagtg tgggcaaaa 
      atggacatc gaggagttc actcagttt ggccctctt 
      gtggggaaa gtggtcaag gaggtggat atttcggaa 
      gattcgaac atgagagat atttgggag atttttgtt 
      gatagcaag aatgtttat tttagtacc gaaaatttg 
      gaaacgtcg aattggatg cggtttgtt cgaccggct 
      ccaaccaga gacgataga aacattact gtggtggca 
      aaggacagt tctctctac tttgttagt attaaagcg 
      atcaaaaac ggggaagag ttgctgtac tggcaggat 
      tccagcatt ccgagcaag aaaaaaatg gagaaaaat 
      tcttgtggt ggttgtaat atgacgttt gctcatccg 
      ctctactac cgaacacat tgctcagta ttccacgac 
      attcgtttt agtcttact attaggaaa taccattgt 
      aaagtttgt ggggctgca gtgttgggt aaagaaaat 
      attatgaaa catgcagct gagttgcat aatggacag 
      ggggcctat cagtgccag ttttgtaaa aagttcttt 
      ttaagactg aattatcta gagatgcat cgtaattat 
      ggctgttct gctaatcca caccgagct aggcctttg 
      tgtgatttc tgtgggcga aaattttgt caaccccaa 
      aagttgaag gttcatatt aaaagaatg cacagtgat 
      ttttccgaa gttttgaag gaattccag tgcaaaaac 
      tgtctgaaa attctcggc tcgcgagca gctctacaa 
      aggcatttg aaggaagtt caccagaaa caaatggac 
      ggtgcttgt tcgtgttct cgttgtggc aaacatttc 
      caaaataaa tcaaattta aaaattcac atgttgact 
      cacagtggg attaaaccc ttcaagtgt gttgtaaag 
      agttgtaat gcggcgttt acaacgaaa cagtgtcta 
      cagttccac tataagaag gttcacagt tttactgag 
      gacgcgatg cctaagatt gaacgaagc attgactac 
      acttttgag gcctacagt ggattagag gaccaggag 
      gataaaggc aaaaatgag atgaatacg caacaaagt 
      gacttgaat gcgacacct cttgaggag gaaagtgac 
      ggggatgag accagtatg gacagcaaa aacgtggac 
      gacccatcc ttacccaac tccccgatg caaccaccc 
      gacgacacc tcccccgac cccaaccct cgaacctcc 
      ccaacgctg gaatcctac aacttaaac accgtaaac 
      gcaaccatg aaaatcctc agcaaaggt tcaaaaaaa 
      tggattggc gaaccacct ctccaaccc ctcaaaccc 
      gagatctac caaatcgag caccaagtc accgaaacc 
      caatacgac cgcccaaaa tcaaaccta accgacttc 
      acacgccac gaagcctca aacgccagt ctcctggtg 
      gaagcagcc ctggactcc gtctgcagc gaaccgaac 
      atagacatc gacgtgagc acaacaccg aattgcacc 
      gacgccttg gtcaacaat ttatacacg ttggctcct 
      tcggacacc cttccggac gtcaccacg tacagcgaa 
      acggtctgt aacataaac gaaccgcgc gatatcaat 
      ttaatttca ccgtcggtc aacgaccac atttccgta 
      acggatgag ttgaatgat gagttacga cacggccag 
      aacatcggc atggattat tccgggttc caccaggag 
      gacttcagt ccggggaac agcccggaa atgcacccg 
      cgcgccaat ttcgtccgg agttacatc aatacgccc 
      aactttggg cccaagaat ttatcaccg gttccggtg 
      cccagtcca ccacgttac gacttcggt cattccgtc 
      aacaccgat catctctca tcggatgat agcaacgga 
      atggccgcg caaaatcta agcctacat tccaataag 
      cacgacata cagttggat ttgtcgatt tacaaggcg 
      ccctataat ttgcaaaat cgcaaagag tttagggtg 
      aaattcgac gatttggac gcaaagtcc aagtatagt 
      ttaatcgag ccgggcaaa agttacgtg gaggaggaa 
      aataacgca ctggatggg gaacaagtg attgaccag 
      aacgaactc tccggcaat attgttgtg gatttgaag 
      gagaaagag gacgaacat gagcgtaat aaatacccc 
      gatgatctc tcgactgat attaggact aataagttt 
      gatattgat ctcgattta aggctgaaa agttacgac 
      atggagggc gaaaatttg cgaacgcgc gggttgtat 
      gaatcaaac tacgaaaaa aactacgaa attatcgac 
      aatattgag aatcgcaag ccttacgac ccagcgctt 
      gacggtgat ttccgcacc gatcgtaac tttgagcct 
      ctagtgctc aactcctcc gagctacaa ggcctggat 
      atgtcggct cggagcttc cacaactat tccaacatc 
      aaccgttac caccacctc taccccgag gttgaccgg 
      gttgatcta cggttgaat tacagtccc ccagctccg 
      ccttacacc cacgccgat atcctccgt gtggtttcg 
      ttagatttg acacctcca ggacgtcat agcgtcgat 
      ttgagtcta cggacgcat ccgttgcac cagatcgcc 
      aattcgagg ttgctttca gagcacgct ccgcaccgt 
      ctgttgacg tccgatttg agtgggagg attttgtcg 
      gatcatacg actaatcgc attttgtcg aataatgat 
      cagttgggg agtaaccat ttgttgggg gctgatcag 
      aggttgttg ggggaggag tcgcggatt atttccgag 
      caaagtcgg ctgattggg gatgggagg attttggcg 
      ccgacgacg ccaggtggg gccgtgtcg ccgattccg 
      ttcagtggg tattcggtg tcgcagagt ccgtatcat 
      ccgacaccg ttggcacct aggccgcat gttacgtca 
      ccgacacct acaccgtac catcattac tccacttat 
      tattgatgt ggacgggga agtatttga atgcgacgg 
      acatacagg ttgtc
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