Status
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Gene information
General information
TC010321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451153.1: 3279..9500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010321
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04808

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010321 RA
      MTQTEPERE KSLQEYRKK LVEHKEVES RLKEMREQL 
      KDLTKQYDK SENDLKALQ SMGQIVGEV LKQLTEEKF 
      IVKATNGPR YVVGCRRQL DKTKLKAGT RVALDMTTL 
      TIMRYLPRE VDPLVYNMS HEDPGDVTY SAIGGLSEQ 
      IRELREVIE LPLMNPELF MRVGITPPK GCLLYGPPG 
      TGKTLLARA VASQLDANF LKVVSSAIV DKYIGESAR 
      LIREMFNYA KDHQPCIIF MDEIDAIGG RRFSEGTSA 
      DREIQRTLM ELLNQMDGF DSLGQVKMI MATNRPDTL 
      DPALLRPGR LDRKIEIPL PNEQARLEI LKIHAGPIA 
      KHGEIDYEA IVKLSDTFN GADLRNVCT EAGLFAIRS 
      EREYVIQED FMKAVRKVA DNKKLESKL DYKPV
    CDS sequence
    • >TC010321 RA
      atgacacaa actgagccc gaacgcgag aaaagcctc 
      caggaatac cggaaaaaa cttgtggaa cacaaagaa 
      gtcgagtct cgcttgaaa gagatgcga gaacagtta 
      aaagaccta acaaaacaa tacgacaag tcagagaat 
      gaccttaag gcgctgcaa agcatgggc caaattgtt 
      ggcgaggtt ttgaaacag ctaactgag gagaaattt 
      attgttaag gcaacgaat ggcccccgt tacgtggtg 
      ggctgtcgc cggcaactt gataagacc aagttgaaa 
      gcagggact cgtgttgct ctcgacatg accacgttg 
      acaataatg cgttatttg ccccgagaa gtcgacccc 
      cttgtttac aacatgagt catgaggac cccggcgat 
      gtcacatac tcggcaatt ggtggtttg tccgagcaa 
      attcgcgag ctccgtgaa gtcatagag ttgcccctc 
      atgaacccc gaactgttc atgagggtg ggcataacc 
      cccccgaaa ggttgtctc ctgtacggc cccccaggc 
      actggcaaa accctccta gcccgggct gtggcctca 
      cagctggac gcaaatttc ctcaaagtt gtctcaagt 
      gctatcgtt gataaatac attggagag tcggctcga 
      ctcattcga gaaatgttt aattatgcg aaagatcat 
      cagccttgc attattttc atggatgag atcgatgct 
      attgggggt cggaggttt tccgagggg acgtcagct 
      gatcgggaa attcagcgg actttgatg gagttgttg 
      aatcagatg gatgggttt gattctctt ggtcaagtt 
      aaaatgatt atggccacg aataggccc gatactttg 
      gatcctgct ttgttgcgt ccaggtcgt ctggatcgt 
      aaaattgaa atcccgctt cctaatgaa caagcccga 
      cttgaaatt ttgaaaatc catgctggg cctatagcg 
      aaacatggg gaaattgat tatgaagcg attgttaag 
      ctgtctgac acgttcaat ggcgctgac ttaagaaac 
      gtatgcact gaggctggt ttgtttgcc attcgatct 
      gaacgggaa tacgtaatc caagaagac tttatgaag 
      gctgtcagg aaggtcgcc gacaacaaa aaattagag 
      agtaaactt gattataaa ccagtttaa
    mRNA sequence
    • >TC010321 RA
      ctgctgtca aaaccaaaa gtgcggtgt tgtggtgtt 
      ctgccagtc attgtgcct ttagtgaaa taactttcg 
      aaaatcctg actaaaaag tgagtcaaa atgacacaa 
      actgagccc gaacgcgag aaaagcctc caggaatac 
      cggaaaaaa cttgtggaa cacaaagaa gtcgagtct 
      cgcttgaaa gagatgcga gaacagtta aaagaccta 
      acaaaacaa tacgacaag tcagagaat gaccttaag 
      gcgctgcaa agcatgggc caaattgtt ggcgaggtt 
      ttgaaacag ctaactgag gagaaattt attgttaag 
      gcaacgaat ggcccccgt tacgtggtg ggctgtcgc 
      cggcaactt gataagacc aagttgaaa gcagggact 
      cgtgttgct ctcgacatg accacgttg acaataatg 
      cgttatttg ccccgagaa gtcgacccc cttgtttac 
      aacatgagt catgaggac cccggcgat gtcacatac 
      tcggcaatt ggtggtttg tccgagcaa attcgcgag 
      ctccgtgaa gtcatagag ttgcccctc atgaacccc 
      gaactgttc atgagggtg ggcataacc cccccgaaa 
      ggttgtctc ctgtacggc cccccaggc actggcaaa 
      accctccta gcccgggct gtggcctca cagctggac 
      gcaaatttc ctcaaagtt gtctcaagt gctatcgtt 
      gataaatac attggagag tcggctcga ctcattcga 
      gaaatgttt aattatgcg aaagatcat cagccttgc 
      attattttc atggatgag atcgatgct attgggggt 
      cggaggttt tccgagggg acgtcagct gatcgggaa 
      attcagcgg actttgatg gagttgttg aatcagatg 
      gatgggttt gattctctt ggtcaagtt aaaatgatt 
      atggccacg aataggccc gatactttg gatcctgct 
      ttgttgcgt ccaggtcgt ctggatcgt aaaattgaa 
      atcccgctt cctaatgaa caagcccga cttgaaatt 
      ttgaaaatc catgctggg cctatagcg aaacatggg 
      gaaattgat tatgaagcg attgttaag ctgtctgac 
      acgttcaat ggcgctgac ttaagaaac gtatgcact 
      gaggctggt ttgtttgcc attcgatct gaacgggaa 
      tacgtaatc caagaagac tttatgaag gctgtcagg 
      aaggtcgcc gacaacaaa aaattagag agtaaactt 
      gattataaa ccagtttaa taatgtatt ttaaataat 
      ttaataaaa tttattact cgtggtcaa atttatctt 
      ctgtgtgcg ggtgcttag ttttagacg tctggtctc 
      tggcacttg ctattctct gattaatac tttgatttt 
      taccaacga tgcttggag atctgctac tgtgactct 
      ttccatcgg agctggcat gtgaagtgg tgtcgtttg 
      tcgcttcat gtattatac tgaagtgtc tgtcttcag 
      tactagtcc ctggaacgc tgtgttgtc gaaactttt 
      ggctgcttc caacaacag gatactcct taagactcg 
      attgttcgg atggttccg gctcttgag tgacgttca 
      cgtcttcga tttcggagt aaactgtgt cgtggactg 
      ttcttaatt tttcgcgtt gttttcgct ggatttttt 
      tgcgtatta tttgcttct gaacctctg gtaagacga 
      agtggtgga cgaggtccg ccacgattt cttcttcgt 
      cgttgatgg ttatattgg ttctgaaat atcacattt 
      tattaaata aatacaagt gtttcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY